RorugMtG0001800

DNA-directed RNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000009
OG0004622
Physical Location & Seq
Reverse (-)
109770 .. 111719
1950 bp
Loading structure...
UTR
Exon/CDS
Intron
RorugMtG0001800.1

Sequence Viewer

Length: 1950 bp
ATGGTACTCGAATTCATTTACGATCCCGAGTTTCCAGACACATCGCACTTCCGCTCGGGTCGAGGCCGCCACTCGGCCCTAAGACGGATCAAAGAAGAGTGGGGAACCTCTCGCTGGTTTTTGGAATTCGACATCAGGAAGTGTTTTCACACCATCGACCGACATCGACTCATTCCAATCTTTAAAGAAGAGATCGACGATCCCAAGTTCTTTTACCCCATTCAGAAAGTCTTTTCCGCCGGACGCCTCGTAGGAGGTGAGAAGGGCCCTTATTCCGTCCCACACAGTGTACTACTATCGGCCCTACCAGGCAACATCTACCTACACAAGCTCGATCAGGAGATAGGGAGGATCCGACAGAAGTACGAAATTCCGGTTGTTCAGAGAATCGGATCGGTTCTATTAAGGACAGGTCGTATTGATGACCAAGAAAACTCTGGAGAAGAAGCAAGCTTCAACGTTCCCCAAGACAACAGAGCCATCATTGTGGGGAGGGTAAAGAGCATCCAACGCAAAGCGGCCTTAAATTCCCTTGTTTCGTCGTGGCACACCCCCCCCACAAGCACCCCCCGGCTCAGGGGGGACCAGAAAACGCCTTTCTTTTTCCCCCCTTCGTCGGCCCTTGCCGCCTTCCTTAACAAGCCCTCGAGCCTCCTTTGCGCCGCCTTCCTCATAGAAGCTGCCGGGTTGACCCCGAAGGCCGAATTCTATGGTAGAGAACGCTGTAATAATAATTGGGCCATGAGAGACCTTATTAAGTATTGCAAAAGAAAGGGCCTGCTGATAGAGCTGGGCGGGGAGGCGATACTAGTTATCAGGTCAGAGAGACGCCTGGCCCGTAAGCTGGCCCCCTTAAAAACCCATTACTTAATAAGGATTTGTTACGCGCGATATGCCGACGACTCACTACTGGGAATCGTGGGTGCCGTAGAGCTTCTCATAGAAATACAAAAACGTATCGCCCACTTCCTACAATCCGGCCTGAACCTTTGGGTAGGCTCTGCAGGATCAACAACAATAGCTGCACGGAGTACGGTAGAATTTCCCGGTACGGTCATTCGGGAAGTCCCTCCGAGGACGACTCCCATACAATTCTTGCGAGAGCTGGAGAAGCGTCTACGGGTAAAGCACCGTATCCATATAACTGCTTGCCACCTACGCTCCACCATCCATTCCAAGTTTAGGAACCTAGGTAATAGTATCCCGATCAAACAGCTGACGAAGGGGATGAGCGAAACAGGGAGTCTACTGGACGGGGTTCAACTAGCGGACACTCTTGGAACAGCTGGAGTAAGAAGTCCCCAAGTGAGCGTATTATGGGGGACCGTCAAGCACATCCGGCAAGAATCAAAGGGGATCCCGTTGTTGCATAGCTCAGGTCGGAGCAAGGCGCCATCGGACGTTCAACAGGCAGTCTCACGATCGGGCATGGGTGTCCGGAAGTTGTCATTGTACACTCCCCCGGGTCGAAAGGCGGCGGAGGAAGGAGGAGGACACTGGGCGGGATCTATCAGCAGCGAATTCCCTATACAGATAGAGGCGCCTATCAAAAAGATACTCCGAAGGCTTCGGGATCGAGGTATCATTAGCCGAAGAAGACCCTGGCCAATCCACGTGGCCTGCTTGACGAACGTCAGCGACGGAGACATCGTAAATTGGTCCGCGGGCATCGCGATAAGTCCTCTGTCCTACTACAGGTGCCGCGACAACCTTTACCAAGTCCGAACGATTGTCGACCACCAGATCCGCTGGTCTGCAATATTCACCCTAGCCCACAAGCACAAATCCTCGGCGCGGAATATAATCCCAAAGTACTCCAAAGACTCAAATATAGTAAATCAAGAAGGTGGTAAGACCCTTGCAGAGTTCCCCAACAGCATAGAGCTGGGGAAGCTCGGACCCGGTCAAGATCCGAACAACAAGGAGCACTCAACTACTAGTCTAGTCTAG

Protein Analysis

649

Amino Acids

72.81

Weight (kDa)

10.05

Isoelectric Point (pI)

52.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Intron_maturas2 PF01348 511 - 606 4e-11 Type II intron maturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 923, 1390, 1540, 1698
AccBSI CCGCTC 1 cut(s) 54
AccI GTMKAC 3 cut(s) 1117, 1246, 1734
AccII CGCG 6 cut(s) 887, 889, 1664, 1673, 1704, 1795
AccIII TCCGGA 1 cut(s) 1437
AclI AACGTT 1 cut(s) 459
AcoI YGGCCR 1 cut(s) 1604
AcsI RAATTY 7 cut(s) 11, 125, 369, 526, 704, 1040, 1520
AcvI CACGTG 1 cut(s) 1615
AcyI GRCGYC 4 cut(s) 244, 829, 1391, 1541
AdeI CACNNNGTG 1 cut(s) 287
AfaI GTAC 7 cut(s) 6, 291, 365, 1033, 1051, 1454, 1814
AgsI TTSAA 3 cut(s) 457, 1262, 1406
AhlI ACTAGT 2 cut(s) 808, 1937
AjnI CCWGG 3 cut(s) 307, 831, 1601
Alw21I GWGCWC 1 cut(s) 1929
Alw26I GTCTC 4 cut(s) 741, 820, 1420, 1638
Ama87I CYCGRG 4 cut(s) 26, 55, 646, 1462
Aor13HI TCCGGA 1 cut(s) 1437
ApaI GGGCCC 1 cut(s) 269
ApeKI GCWGC 3 cut(s) 680, 1022, 1515
ApoI RAATTY 7 cut(s) 11, 125, 369, 526, 704, 1040, 1520
AspA2I CCTAGG 1 cut(s) 1189
AspLEI GCGC 5 cut(s) 662, 889, 1393, 1543, 1795
AsuC2I CCSGG 6 cut(s) 571, 685, 1047, 1463, 1464, 1902
AsuHPI GGTGA 2 cut(s) 269, 1756
AvaI CYCGRG 4 cut(s) 26, 55, 646, 1462
AvaII GGWCC 4 cut(s) 583, 1323, 1659, 1898
AvrII CCTAGG 1 cut(s) 1189
BaeGI GKGCMC 1 cut(s) 269
BalI TGGCCA 1 cut(s) 1606
BamHI GGATCC 2 cut(s) 351, 1356
BanI GGYRCC 4 cut(s) 923, 1390, 1540, 1698
BanII GRGCYC 1 cut(s) 269
BbrPI CACGTG 1 cut(s) 1615
BbsI GAAGAC 1 cut(s) 1603
Bbv12I GWGCWC 1 cut(s) 1929
BbvI GCAGC 3 cut(s) 667, 1009, 1527
BccI CCATC 4 cut(s) 161, 488, 1175, 1402
BceAI ACGGC 1 cut(s) 911
BciT130I CCWGG 3 cut(s) 309, 833, 1603
BciVI GTATCC 2 cut(s) 1145, 1211
BcnI CCSGG 6 cut(s) 571, 685, 1047, 1463, 1464, 1902
BcoDI GTCTC 4 cut(s) 741, 820, 1420, 1638
BcuI ACTAGT 2 cut(s) 808, 1937
BfaI CTAG 7 cut(s) 809, 1190, 1265, 1769, 1938, 1943, 1948
BfmI CTRYAG 2 cut(s) 1002, 1693
BfoI RGCGCY 2 cut(s) 1394, 1544
BfuI GTATCC 2 cut(s) 1145, 1211
BisI GCNGC 9 cut(s) 67, 519, 627, 663, 681, 1023, 1476, 1516, 1702
BlnI CCTAGG 1 cut(s) 1189
BlsI GCNGC 9 cut(s) 68, 520, 628, 664, 682, 1024, 1477, 1517, 1703
BmcAI AGTACT 1 cut(s) 1814
Bme1390I CCNGG 9 cut(s) 309, 571, 685, 833, 1047, 1463, 1464, 1603, 1902
Bme18I GGWCC 4 cut(s) 583, 1323, 1659, 1898
BmeT110I CYCGRG 4 cut(s) 26, 55, 646, 1462
BmrFI CCNGG 9 cut(s) 309, 571, 685, 833, 1047, 1463, 1464, 1603, 1902
BmrI ACTGGG 2 cut(s) 920, 1507
BmsI GCATC 2 cut(s) 513, 1677
BmuI ACTGGG 2 cut(s) 920, 1507
BoxI GACNNNNGTC 1 cut(s) 1631
BpiI GAAGAC 1 cut(s) 1603
BplI GAGNNNNNCTC 2 cut(s) 1066, 1098
BpmI CTGGAG 3 cut(s) 459, 1127, 1308
Bpu10I CCTNAGC 2 cut(s) 575, 1375
BpuMI CCSGG 6 cut(s) 571, 685, 1047, 1463, 1464, 1902
BsaAI YACGTR 1 cut(s) 1615
BsaHI GRCGYC 4 cut(s) 244, 829, 1391, 1541
BsaI GGTCTC 1 cut(s) 741
BsaJI CCNNGG 8 cut(s) 569, 1073, 1189, 1461, 1462, 1601, 1662, 1788
BsaWI WCCGGW 2 cut(s) 373, 1437
BsaXI ACNNNNNCTCC 4 cut(s) 1145, 1175, 1635, 1665
Bse1I ACTGG 3 cut(s) 915, 1254, 1502
BseAI TCCGGA 1 cut(s) 1437
BseBI CCWGG 3 cut(s) 309, 833, 1603
BseDI CCNNGG 8 cut(s) 569, 1073, 1189, 1461, 1462, 1601, 1662, 1788
BseGI GGATG 4 cut(s) 504, 1167, 1233, 1335
BseMII CTCAG 2 cut(s) 589, 1389
BseNI ACTGG 3 cut(s) 915, 1254, 1502
BseRI GAGGAG 1 cut(s) 1503
BseSI GKGCMC 1 cut(s) 269
BseXI GCAGC 3 cut(s) 667, 1009, 1527
BseYI CCCAGC 2 cut(s) 790, 1885
BsgI GTGCAG 1 cut(s) 1008
Bsh1236I CGCG 6 cut(s) 887, 889, 1664, 1673, 1704, 1795
Bsh1285I CGRYCG 2 cut(s) 160, 1424
BshNI GGYRCC 4 cut(s) 923, 1390, 1540, 1698
BsiEI CGRYCG 2 cut(s) 160, 1424
BsiHKAI GWGCWC 1 cut(s) 1929
BsiHKCI CYCGRG 4 cut(s) 26, 55, 646, 1462
BslFI GGGAC 5 cut(s) 263, 596, 1052, 1284, 1336
BsmAI GTCTC 4 cut(s) 741, 820, 1420, 1638
BsmBI CGTCTC 1 cut(s) 820
BsmFI GGGAC 5 cut(s) 263, 596, 1052, 1284, 1336
Bso31I GGTCTC 1 cut(s) 741
BsoBI CYCGRG 4 cut(s) 26, 55, 646, 1462
Bsp120I GGGCCC 1 cut(s) 265
Bsp1286I GDGCHC 2 cut(s) 269, 1929
Bsp13I TCCGGA 1 cut(s) 1437
Bsp1407I TGTACA 1 cut(s) 1452
Bsp68I TCGCGA 1 cut(s) 1673
BspCNI CTCAG 2 cut(s) 588, 1388
BspEI TCCGGA 1 cut(s) 1437
BspFNI CGCG 6 cut(s) 887, 889, 1664, 1673, 1704, 1795
BspMAI CTGCAG 1 cut(s) 1006
BspT107I GGYRCC 4 cut(s) 923, 1390, 1540, 1698
BspTNI GGTCTC 1 cut(s) 741
BsrBI CCGCTC 1 cut(s) 54
BsrGI TGTACA 1 cut(s) 1452
BsrI ACTGG 3 cut(s) 915, 1254, 1502
BssECI CCNNGG 8 cut(s) 569, 1073, 1189, 1461, 1462, 1601, 1662, 1788
BssNI GRCGYC 4 cut(s) 244, 829, 1391, 1541
BssT1I CCWWGG 1 cut(s) 1189
Bst2UI CCWGG 3 cut(s) 309, 833, 1603
Bst4CI ACNGT 5 cut(s) 287, 1036, 1054, 1133, 1327
Bst6I CTCTTC 2 cut(s) 90, 183
BstACI GRCGYC 4 cut(s) 244, 829, 1391, 1541
BstAUI TGTACA 1 cut(s) 1452
BstBAI YACGTR 1 cut(s) 1615
BstC8I GCNNGC 6 cut(s) 451, 779, 846, 1150, 1621, 1666
BstDEI CTNAG 3 cut(s) 80, 575, 1375
BstDSI CCRYGG 1 cut(s) 1662
BstENI CCTNNNNNAGG 1 cut(s) 1693
BstF5I GGATG 4 cut(s) 504, 1167, 1233, 1335
BstFNI CGCG 6 cut(s) 887, 889, 1664, 1673, 1704, 1795
BstH2I RGCGCY 2 cut(s) 1394, 1544
BstHHI GCGC 5 cut(s) 662, 889, 1393, 1543, 1795
BstMAI GTCTC 4 cut(s) 741, 820, 1420, 1638
BstMCI CGRYCG 2 cut(s) 160, 1424
BstNI CCWGG 3 cut(s) 309, 833, 1603
BstPAI GACNNNNGTC 1 cut(s) 1631
BstSCI CCNGG 9 cut(s) 307, 569, 683, 831, 1045, 1461, 1462, 1601, 1900
BstSFI CTRYAG 2 cut(s) 1002, 1693
BstSLI GKGCMC 1 cut(s) 269
BstUI CGCG 6 cut(s) 887, 889, 1664, 1673, 1704, 1795
BstV1I GCAGC 3 cut(s) 667, 1009, 1527
BstV2I GAAGAC 1 cut(s) 1603
BstX2I RGATCY 5 cut(s) 351, 1356, 1505, 1743, 1909
BstXI CCANNNNNNTGG 1 cut(s) 487
BstYI RGATCY 5 cut(s) 351, 1356, 1505, 1743, 1909
BsuI GTATCC 2 cut(s) 1145, 1211
BtgI CCRYGG 1 cut(s) 1662
BtgZI GCGATG 2 cut(s) 27, 1654
BtsCI GGATG 4 cut(s) 504, 1167, 1233, 1335
BtsIMutI CAGTG 2 cut(s) 292, 1495
BtuMI TCGCGA 1 cut(s) 1673
Cac8I GCNNGC 6 cut(s) 451, 779, 846, 1150, 1621, 1666
CfoI GCGC 5 cut(s) 662, 889, 1393, 1543, 1795
Cfr42I CCGCGG 1 cut(s) 1665
Cfr9I CCCGGG 1 cut(s) 1462
CseI GACGC 3 cut(s) 252, 837, 1103
Csp6I GTAC 7 cut(s) 5, 290, 364, 1032, 1050, 1453, 1813
CspCI CAANNNNNGTGG 2 cut(s) 1596, 1631
CviAII CATG 2 cut(s) 742, 1429
CviQI GTAC 7 cut(s) 5, 290, 364, 1032, 1050, 1453, 1813
DdeI CTNAG 3 cut(s) 80, 575, 1375
DinI GGCGCC 2 cut(s) 1392, 1542
DraI TTTAAA 1 cut(s) 184
DraIII CACNNNGTG 1 cut(s) 287
EaeI YGGCCR 1 cut(s) 1604
Eam1104I CTCTTC 2 cut(s) 90, 183
EarI CTCTTC 2 cut(s) 90, 183
EciI GGCGGA 2 cut(s) 226, 1493
Eco130I CCWWGG 1 cut(s) 1189
Eco24I GRGCYC 1 cut(s) 269
Eco31I GGTCTC 1 cut(s) 741
Eco47I GGWCC 4 cut(s) 583, 1323, 1659, 1898
Eco72I CACGTG 1 cut(s) 1615
Eco88I CYCGRG 4 cut(s) 26, 55, 646, 1462
EcoNI CCTNNNNNAGG 1 cut(s) 1693
EcoO109I RGGNCCY 3 cut(s) 265, 266, 775
EcoRI GAATTC 4 cut(s) 11, 125, 704, 1520
EcoRII CCWGG 3 cut(s) 307, 831, 1601
EcoT14I CCWWGG 1 cut(s) 1189
EcoT38I GRGCYC 1 cut(s) 269
EgeI GGCGCC 2 cut(s) 1392, 1542
EheI GGCGCC 2 cut(s) 1392, 1542
ErhI CCWWGG 1 cut(s) 1189
Esp3I CGTCTC 1 cut(s) 820
FaeI CATG 2 cut(s) 745, 1432
FaqI GGGAC 5 cut(s) 263, 596, 1052, 1284, 1336
FatI CATG 2 cut(s) 741, 1428
FauI CCCGC 3 cut(s) 788, 1495, 1657
FblI GTMKAC 3 cut(s) 1117, 1246, 1734
Fnu4HI GCNGC 9 cut(s) 67, 519, 627, 663, 681, 1023, 1476, 1516, 1702
FokI GGATG 4 cut(s) 491, 1154, 1240, 1322
FriOI GRGCYC 1 cut(s) 269
Fsp4HI GCNGC 9 cut(s) 67, 519, 627, 663, 681, 1023, 1476, 1516, 1702
FspBI CTAG 7 cut(s) 809, 1190, 1265, 1769, 1938, 1943, 1948
GlaI GCGC 5 cut(s) 661, 888, 1392, 1542, 1794
GluI GCNGC 9 cut(s) 67, 519, 627, 663, 681, 1023, 1476, 1516, 1702
GsaI CCCAGC 2 cut(s) 794, 1889
GsuI CTGGAG 3 cut(s) 459, 1127, 1308
HaeII RGCGCY 2 cut(s) 1394, 1544
HgaI GACGC 3 cut(s) 252, 837, 1103
HhaI GCGC 5 cut(s) 662, 889, 1393, 1543, 1795
Hin1I GRCGYC 4 cut(s) 244, 829, 1391, 1541
Hin1II CATG 2 cut(s) 745, 1432
Hin6I GCGC 5 cut(s) 660, 887, 1391, 1541, 1793
HinP1I GCGC 5 cut(s) 660, 887, 1391, 1541, 1793
HincII GTYRAC 2 cut(s) 690, 1735
HindII GTYRAC 2 cut(s) 690, 1735
HindIII AAGCTT 1 cut(s) 451
HinfI GANTC 8 cut(s) 168, 387, 902, 915, 1081, 1243, 1346, 1823
HphI GGTGA 2 cut(s) 269, 1756
Hpy166II GTNNAC 6 cut(s) 290, 690, 1118, 1247, 1455, 1735
Hpy8I GTNNAC 6 cut(s) 290, 690, 1118, 1247, 1455, 1735
Hpy99I CGWCG 5 cut(s) 200, 544, 619, 902, 1643
HpyAV CCTTC 9 cut(s) 256, 621, 640, 676, 691, 1216, 1478, 1557, 1838
HpyCH4III ACNGT 5 cut(s) 287, 1036, 1054, 1133, 1327
HpyCH4IV ACGT 5 cut(s) 459, 955, 1401, 1614, 1632
HpyCH4V TGCA 6 cut(s) 765, 1004, 1025, 1369, 1757, 1862
HpyF3I CTNAG 3 cut(s) 80, 575, 1375
HpySE526I ACGT 5 cut(s) 459, 955, 1401, 1614, 1632
Hsp92I GRCGYC 4 cut(s) 244, 829, 1391, 1541
Hsp92II CATG 2 cut(s) 745, 1432
HspAI GCGC 5 cut(s) 660, 887, 1391, 1541, 1793
KasI GGCGCC 2 cut(s) 1390, 1540
Kpn2I TCCGGA 1 cut(s) 1437
KspI CCGCGG 1 cut(s) 1665
LmnI GCTCC 3 cut(s) 1166, 1383, 1924
Lsp1109I GCAGC 3 cut(s) 667, 1009, 1527
LweI GCATC 2 cut(s) 513, 1677
MaeI CTAG 7 cut(s) 809, 1190, 1265, 1769, 1938, 1943, 1948
MaeII ACGT 5 cut(s) 459, 955, 1401, 1614, 1632
MaeIII GTNAC 1 cut(s) 881
MbiI CCGCTC 1 cut(s) 54
MboII GAAGA 5 cut(s) 107, 200, 455, 1605, 1608
MflI RGATCY 5 cut(s) 351, 1356, 1505, 1743, 1909
MhlI GDGCHC 2 cut(s) 269, 1929
MlsI TGGCCA 1 cut(s) 1606
MluNI TGGCCA 1 cut(s) 1606
Mly113I GGCGCC 2 cut(s) 1391, 1541
MlyI GAGTC 5 cut(s) 162, 896, 1075, 1252, 1817
MmeI TCCRAC 3 cut(s) 379, 532, 1361
Mox20I TGGCCA 1 cut(s) 1606
MroI TCCGGA 1 cut(s) 1437
MscI TGGCCA 1 cut(s) 1606
MseI TTAA 7 cut(s) 183, 404, 524, 636, 756, 854, 869
MslI CAYNNNNRTG 1 cut(s) 485
Msp20I TGGCCA 1 cut(s) 1606
MspA1I CMGCKG 4 cut(s) 1216, 1286, 1664, 1749
MspR9I CCNGG 9 cut(s) 309, 571, 685, 833, 1047, 1463, 1464, 1603, 1902
MvaI CCWGG 3 cut(s) 309, 833, 1603
MvnI CGCG 6 cut(s) 887, 889, 1664, 1673, 1704, 1795
NarI GGCGCC 2 cut(s) 1391, 1541
NciI CCSGG 6 cut(s) 571, 685, 1047, 1463, 1464, 1902
NlaIII CATG 2 cut(s) 745, 1432
NmeAIII GCCGAG 2 cut(s) 53, 1769
NruI TCGCGA 1 cut(s) 1673
PaeR7I CTCGAG 1 cut(s) 646
PcsI WCGNNNNNNNCGW 3 cut(s) 835, 924, 1647
PfeI GAWTC 3 cut(s) 387, 915, 1346
PflFI GACNNNGTC 1 cut(s) 1902
PkrI GCNGC 9 cut(s) 68, 520, 628, 664, 682, 1024, 1477, 1517, 1703
Ple19I CGATCG 1 cut(s) 1424
PleI GAGTC 5 cut(s) 162, 896, 1075, 1251, 1817
PluTI GGCGCC 2 cut(s) 1394, 1544
PmaCI CACGTG 1 cut(s) 1615
PmlI CACGTG 1 cut(s) 1615
PpsI GAGTC 5 cut(s) 162, 896, 1075, 1251, 1817
Ppu21I YACGTR 1 cut(s) 1615
PshAI GACNNNNGTC 1 cut(s) 1631
Psp1406I AACGTT 1 cut(s) 459
Psp6I CCWGG 3 cut(s) 307, 831, 1601
PspCI CACGTG 1 cut(s) 1615
PspFI CCCAGC 2 cut(s) 790, 1885
PspGI CCWGG 3 cut(s) 307, 831, 1601
PspOMI GGGCCC 1 cut(s) 265
PspXI VCTCGAGB 1 cut(s) 646
PstI CTGCAG 1 cut(s) 1006
PsuI RGATCY 5 cut(s) 351, 1356, 1505, 1743, 1909
PsyI GACNNNGTC 1 cut(s) 1902
PvuI CGATCG 1 cut(s) 1424
PvuII CAGCTG 2 cut(s) 1216, 1286
RruI TCGCGA 1 cut(s) 1673
RsaI GTAC 7 cut(s) 6, 291, 365, 1033, 1051, 1454, 1814
RsaNI GTAC 7 cut(s) 5, 290, 364, 1032, 1050, 1453, 1813
RseI CAYNNNNRTG 1 cut(s) 485
SacII CCGCGG 1 cut(s) 1665
SalI GTCGAC 1 cut(s) 1733
SaqAI TTAA 7 cut(s) 183, 404, 524, 636, 756, 854, 869
SatI GCNGC 9 cut(s) 67, 519, 627, 663, 681, 1023, 1476, 1516, 1702
ScaI AGTACT 1 cut(s) 1814
SchI GAGTC 5 cut(s) 162, 896, 1075, 1252, 1817
ScrFI CCNGG 9 cut(s) 309, 571, 685, 833, 1047, 1463, 1464, 1603, 1902
SduI GDGCHC 2 cut(s) 269, 1929
SfaNI GCATC 2 cut(s) 513, 1677
SfcI CTRYAG 2 cut(s) 1002, 1693
SfoI GGCGCC 2 cut(s) 1392, 1542
Sfr274I CTCGAG 1 cut(s) 646
Sfr303I CCGCGG 1 cut(s) 1665
SgrBI CCGCGG 1 cut(s) 1665
SinI GGWCC 4 cut(s) 583, 1323, 1659, 1898
SlaI CTCGAG 1 cut(s) 646
SmaI CCCGGG 1 cut(s) 1464
SmiMI CAYNNNNRTG 1 cut(s) 485
SmlI CTYRAG 1 cut(s) 646
SmoI CTYRAG 1 cut(s) 646
SpeI ACTAGT 2 cut(s) 808, 1937
SspDI GGCGCC 2 cut(s) 1390, 1540
SspI AATATT 1 cut(s) 1761
SspMI CTAG 7 cut(s) 809, 1190, 1265, 1769, 1938, 1943, 1948
StyD4I CCNGG 9 cut(s) 307, 569, 683, 831, 1045, 1461, 1462, 1601, 1900
StyI CCWWGG 1 cut(s) 1189
TaaI ACNGT 5 cut(s) 287, 1036, 1054, 1133, 1327
TaiI ACGT 5 cut(s) 462, 958, 1404, 1617, 1635
TaqII GACCGA 1 cut(s) 174
TatI WGTACW 3 cut(s) 289, 1452, 1812
TauI GCSGC 6 cut(s) 69, 521, 629, 665, 1478, 1704
TfiI GAWTC 3 cut(s) 387, 915, 1346
Tru1I TTAA 7 cut(s) 183, 404, 524, 636, 756, 854, 869
Tru9I TTAA 7 cut(s) 183, 404, 524, 636, 756, 854, 869
TscAI CASTG 2 cut(s) 292, 1502
TseI GCWGC 3 cut(s) 680, 1022, 1515
TspDTI ATGAA 1 cut(s) 4
TspGWI ACGGA 4 cut(s) 100, 265, 1042, 1656
TspMI CCCGGG 1 cut(s) 1462
TspRI CASTG 2 cut(s) 292, 1502
Tth111I GACNNNGTC 1 cut(s) 1902
VpaK11BI GGWCC 4 cut(s) 583, 1323, 1659, 1898
XagI CCTNNNNNAGG 1 cut(s) 1693
XapI RAATTY 7 cut(s) 11, 125, 369, 526, 704, 1040, 1520
XcmI CCANNNNNNNNNTGG 1 cut(s) 434
XhoI CTCGAG 1 cut(s) 646
XmaI CCCGGG 1 cut(s) 1462
XmaJI CCTAGG 1 cut(s) 1189
XmiI GTMKAC 3 cut(s) 1117, 1246, 1734
XspI CTAG 7 cut(s) 809, 1190, 1265, 1769, 1938, 1943, 1948
ZrmI AGTACT 1 cut(s) 1814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.