RLG00000001485

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0011605
Physical Location & Seq
Reverse (-)
16230523 .. 16231179
657 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001485

Sequence Viewer

Length: 546 bp
ATGGCGGTAACCACCACCGCCCAGACTTCGGCTTCAACTCCCAAACTCAAGGGCTTTGCCCCAGTCCTCCTACTCCTCCTCATCCTCGTCTTATCAACCATCTTCGGCGGATCTGCGAATCGGATCCGGTTGGTTAAGAATCAGATCCCCAGCTGCTGCGAAATGTCGTCGGAAGCGCAGTGCTCCCAAAACCCCAAGTGCCGGTGGTGCTGCAGCGAGTCCCTGGAAGACATGTGCTTCCCCAAGTCTGAGGCTCTGCGATTGCCCCCTCAAACTAGTATGCGTTGTTATTTGTTCATTGAGGAGGGAAATGAGGAGGAGAAGGAGGAGCTGGAGTTGAATCTGGGGCTATCTTTGGGAGGAAGATTCGGAGGCGGCGTCATACTCAGGGCTGATGAGGGCGTCGTCGCTTCCGATGGAGATGAAGGAGGAATGGAGGAAGAGGAAGGAGCTGCAGATATTGCGGCGGTTGGCGGCGAAGTGGAGGAGATTAGGGAAGCAGAGGAACAGCAGCAGCAACAAGGAGGAAGAGCTGGCATTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

182

Amino Acids

19.4

Weight (kDa)

4.39

Isoelectric Point (pI)

60.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EAR PF07897 106 - 124 9e-07 Ethylene-responsive binding factor-associated repression
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 7 cut(s) 5, 18, 108, 375, 464, 467, 474
AclWI GGATC 4 cut(s) 118, 118, 131, 139
AcyI GRCGYC 2 cut(s) 378, 402
AfiI CCNNNNNNNGG 2 cut(s) 28, 201
AflIII ACRYGT 1 cut(s) 231
AgsI TTSAA 2 cut(s) 36, 340
AhlI ACTAGT 1 cut(s) 275
AjnI CCWGG 1 cut(s) 222
AluBI AGCT 4 cut(s) 153, 331, 452, 533
AluI AGCT 4 cut(s) 153, 331, 452, 533
Alw21I GWGCWC 1 cut(s) 185
AlwI GGATC 4 cut(s) 118, 118, 131, 139
AlwNI CAGNNNCTG 1 cut(s) 156
ApeKI GCWGC 7 cut(s) 153, 156, 210, 213, 452, 511, 514
AspLEI GCGC 1 cut(s) 178
BamHI GGATCC 1 cut(s) 123
BbsI GAAGAC 1 cut(s) 234
Bbv12I GWGCWC 1 cut(s) 185
BbvI GCAGC 7 cut(s) 140, 143, 197, 225, 439, 523, 526
BccI CCATC 2 cut(s) 107, 410
BciT130I CCWGG 1 cut(s) 224
BcuI ACTAGT 1 cut(s) 275
BfaI CTAG 2 cut(s) 276, 544
BfmI CTRYAG 2 cut(s) 211, 453
Bme1390I CCNGG 1 cut(s) 224
BmiI GGNNCC 1 cut(s) 125
BmrFI CCNGG 1 cut(s) 224
BmrI ACTGGG 1 cut(s) 56
BmuI ACTGGG 1 cut(s) 56
BpiI GAAGAC 1 cut(s) 234
BpmI CTGGAG 1 cut(s) 353
BpuEI CTTGAG 1 cut(s) 32
BsaHI GRCGYC 2 cut(s) 378, 402
BsaJI CCNNGG 1 cut(s) 222
BsaWI WCCGGW 1 cut(s) 126
BsaXI ACNNNNNCTCC 4 cut(s) 320, 350, 363, 393
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 201
Bse118I RCCGGY 1 cut(s) 201
Bse1I ACTGG 1 cut(s) 62
BseBI CCWGG 1 cut(s) 224
BseDI CCNNGG 1 cut(s) 222
BseGI GGATG 1 cut(s) 81
BseLI CCNNNNNNNGG 2 cut(s) 28, 201
BseMII CTCAG 2 cut(s) 240, 400
BseNI ACTGG 1 cut(s) 62
BseRI GAGGAG 7 cut(s) 65, 68, 317, 329, 332, 341, 500
BseXI GCAGC 7 cut(s) 140, 143, 197, 225, 439, 523, 526
BseYI CCCAGC 1 cut(s) 149
BsiHKAI GWGCWC 1 cut(s) 185
BsiSI CCGG 2 cut(s) 127, 202
BslFI GGGAC 1 cut(s) 205
BslI CCNNNNNNNGG 2 cut(s) 28, 201
BsmFI GGGAC 1 cut(s) 205
Bsp1286I GDGCHC 1 cut(s) 185
Bsp143I GATC 3 cut(s) 110, 123, 144
BspACI CCGC 7 cut(s) 5, 18, 108, 375, 464, 467, 474
BspCNI CTCAG 2 cut(s) 241, 399
BspLI GGNNCC 1 cut(s) 125
BspMAI CTGCAG 2 cut(s) 215, 457
BspPI GGATC 4 cut(s) 118, 118, 131, 139
BspQI GCTCTTC 1 cut(s) 523
BsrFI RCCGGY 1 cut(s) 201
BsrI ACTGG 1 cut(s) 62
BssAI RCCGGY 1 cut(s) 201
BssECI CCNNGG 1 cut(s) 222
BssMI GATC 3 cut(s) 110, 123, 144
BssNI GRCGYC 2 cut(s) 378, 402
Bst2UI CCWGG 1 cut(s) 224
Bst6I CTCTTC 2 cut(s) 435, 523
BstACI GRCGYC 2 cut(s) 378, 402
BstAPI GCANNNNNTGC 1 cut(s) 461
BstC8I GCNNGC 1 cut(s) 535
BstDEI CTNAG 2 cut(s) 249, 386
BstEII GGTNACC 1 cut(s) 7
BstF5I GGATG 1 cut(s) 81
BstHHI GCGC 1 cut(s) 178
BstKTI GATC 3 cut(s) 113, 126, 147
BstMBI GATC 3 cut(s) 110, 123, 144
BstMWI GCNNNNNNNGC 2 cut(s) 207, 461
BstNI CCWGG 1 cut(s) 224
BstNSI RCATGY 1 cut(s) 235
BstPI GGTNACC 1 cut(s) 7
BstSCI CCNGG 1 cut(s) 222
BstSFI CTRYAG 2 cut(s) 211, 453
BstV1I GCAGC 7 cut(s) 140, 143, 197, 225, 439, 523, 526
BstV2I GAAGAC 1 cut(s) 234
BstX2I RGATCY 3 cut(s) 110, 123, 144
BstYI RGATCY 3 cut(s) 110, 123, 144
BtsCI GGATG 1 cut(s) 81
BtsI GCAGTG 1 cut(s) 185
BtsIMutI CAGTG 1 cut(s) 185
Cac8I GCNNGC 1 cut(s) 535
CaiI CAGNNNCTG 1 cut(s) 156
CfoI GCGC 1 cut(s) 178
Cfr10I RCCGGY 1 cut(s) 201
CseI GACGC 2 cut(s) 367, 391
CviAII CATG 1 cut(s) 232
CviJI RGCY 9 cut(s) 32, 54, 153, 254, 331, 349, 392, 452, 533
CviKI_1 RGCY 9 cut(s) 32, 54, 153, 254, 331, 349, 392, 452, 533
DdeI CTNAG 2 cut(s) 249, 386
DpnI GATC 3 cut(s) 112, 125, 146
DpnII GATC 3 cut(s) 110, 123, 144
Eam1104I CTCTTC 2 cut(s) 435, 523
EarI CTCTTC 2 cut(s) 435, 523
EciI GGCGGA 1 cut(s) 123
Eco91I GGTNACC 1 cut(s) 7
EcoO65I GGTNACC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 222
FaeI CATG 1 cut(s) 235
FaiI YATR 3 cut(s) 233, 281, 383
FaqI GGGAC 1 cut(s) 205
FatI CATG 1 cut(s) 231
FokI GGATG 1 cut(s) 68
FspBI CTAG 2 cut(s) 276, 544
GlaI GCGC 1 cut(s) 177
GsaI CCCAGC 1 cut(s) 153
GsuI CTGGAG 1 cut(s) 353
HapII CCGG 2 cut(s) 127, 202
HgaI GACGC 2 cut(s) 367, 391
HhaI GCGC 1 cut(s) 178
Hin1I GRCGYC 2 cut(s) 378, 402
Hin1II CATG 1 cut(s) 235
Hin6I GCGC 1 cut(s) 176
HinP1I GCGC 1 cut(s) 176
HinfI GANTC 5 cut(s) 118, 139, 218, 340, 366
HpaII CCGG 2 cut(s) 127, 202
Hpy188I TCNGA 6 cut(s) 123, 144, 172, 250, 371, 415
Hpy99I CGWCG 3 cut(s) 172, 407, 410
HpyAV CCTTC 3 cut(s) 316, 419, 440
HpyCH4V TGCA 2 cut(s) 213, 455
HpyF10VI GCNNNNNNNGC 2 cut(s) 207, 461
HpyF3I CTNAG 2 cut(s) 249, 386
Hsp92I GRCGYC 2 cut(s) 378, 402
Hsp92II CATG 1 cut(s) 235
HspAI GCGC 1 cut(s) 176
Kzo9I GATC 3 cut(s) 110, 123, 144
LguI GCTCTTC 1 cut(s) 523
LmnI GCTCC 3 cut(s) 188, 328, 449
Lsp1109I GCAGC 7 cut(s) 140, 143, 197, 225, 439, 523, 526
MaeI CTAG 2 cut(s) 276, 544
MaeIII GTNAC 1 cut(s) 7
MalI GATC 3 cut(s) 112, 125, 146
MboI GATC 3 cut(s) 110, 123, 144
MboII GAAGA 5 cut(s) 94, 239, 375, 452, 540
MflI RGATCY 3 cut(s) 110, 123, 144
MhlI GDGCHC 1 cut(s) 185
MlyI GAGTC 1 cut(s) 227
MmeI TCCRAC 1 cut(s) 150
MseI TTAA 2 cut(s) 135, 540
MspA1I CMGCKG 1 cut(s) 153
MspI CCGG 2 cut(s) 127, 202
MspR9I CCNGG 1 cut(s) 224
MvaI CCWGG 1 cut(s) 224
MwoI GCNNNNNNNGC 2 cut(s) 207, 461
NdeII GATC 3 cut(s) 110, 123, 144
NlaIII CATG 1 cut(s) 235
NlaIV GGNNCC 1 cut(s) 125
NspI RCATGY 1 cut(s) 235
PciI ACATGT 1 cut(s) 231
PciSI GCTCTTC 1 cut(s) 523
PcsI WCGNNNNNNNCGW 2 cut(s) 375, 411
PfeI GAWTC 4 cut(s) 118, 139, 340, 366
PleI GAGTC 1 cut(s) 226
PpsI GAGTC 1 cut(s) 226
PscI ACATGT 1 cut(s) 231
Psp6I CCWGG 1 cut(s) 222
PspEI GGTNACC 1 cut(s) 7
PspFI CCCAGC 1 cut(s) 149
PspGI CCWGG 1 cut(s) 222
PspN4I GGNNCC 1 cut(s) 125
PstI CTGCAG 2 cut(s) 215, 457
PstNI CAGNNNCTG 1 cut(s) 156
PsuI RGATCY 3 cut(s) 110, 123, 144
PvuII CAGCTG 1 cut(s) 153
SapI GCTCTTC 1 cut(s) 523
SaqAI TTAA 2 cut(s) 135, 540
Sau3AI GATC 3 cut(s) 110, 123, 144
SchI GAGTC 1 cut(s) 227
ScrFI CCNGG 1 cut(s) 224
SduI GDGCHC 1 cut(s) 185
SetI ASST 4 cut(s) 155, 333, 454, 535
SfcI CTRYAG 2 cut(s) 211, 453
SmlI CTYRAG 1 cut(s) 47
SmoI CTYRAG 1 cut(s) 47
SpeI ACTAGT 1 cut(s) 275
SsiI CCGC 7 cut(s) 5, 18, 108, 375, 464, 467, 474
SspMI CTAG 2 cut(s) 276, 544
StyD4I CCNGG 1 cut(s) 222
TauI GCSGC 3 cut(s) 378, 467, 477
TfiI GAWTC 4 cut(s) 118, 139, 340, 366
Tru1I TTAA 2 cut(s) 135, 540
Tru9I TTAA 2 cut(s) 135, 540
TscAI CASTG 1 cut(s) 185
TseI GCWGC 7 cut(s) 153, 156, 210, 213, 452, 511, 514
TspDTI ATGAA 2 cut(s) 286, 438
TspRI CASTG 1 cut(s) 185
XceI RCATGY 1 cut(s) 235
XspI CTAG 2 cut(s) 276, 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.