Rroxscaffold_1G00064600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011605
Physical Location & Seq
Reverse (-)
86445965 .. 86447708
1744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00064600.1

Sequence Viewer

Length: 495 bp
ATGGCGGCAACCACCACCGCCAAGACTTCCACTTCAACTCCCAAACTCAAGAGCTTCGCCCCAGTCCTCCTCCTCCTCCTCCTCCTCCTCGTCTTATCAACCTTCTCCTGCGGATCTGCGAATCGGATCCGGTTGGTTAAGAATCAGATCCCCAGCTGCTGCGACATGTCGTCGGAAGCGCAGTGCTCCCAGAACCCCAAGTGCCGGTGGTGCCGCAGCGAGTCCCTGGACGACATGTGCTTCCCCAAGTCCGAGGCTCTGCGATTGCCCCCTCAGAACCACCTTCGGAGATCAGTTGGTGAATCAGAGCAGAAGAATGTGAGGTTGTCCATATATCCAGGGCTTCGAAGACCTATATTGCAGGAGGGGGATGTGGATTCCGTAACAAATTCACAGCTTCCTATATATAGCTATTGCAACACTTCAGCAGCATTAGTAGCGACTACTCAGTACCTGATTAGCTTCACAAACACAAAGCCTCATCCATTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

164

Amino Acids

18.2

Weight (kDa)

9.13

Isoelectric Point (pI)

59.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 210
AciI CCGC 4 cut(s) 5, 18, 111, 214
AclWI GGATC 4 cut(s) 121, 121, 134, 142
AcsI RAATTY 1 cut(s) 388
AcuI CTGAAG 1 cut(s) 408
AfaI GTAC 1 cut(s) 452
AfiI CCNNNNNNNGG 1 cut(s) 204
AflIII ACRYGT 2 cut(s) 165, 234
AgsI TTSAA 1 cut(s) 36
AhdI GACNNNNNGTC 1 cut(s) 169
AjnI CCWGG 2 cut(s) 225, 337
AluBI AGCT 5 cut(s) 54, 156, 397, 411, 462
AluI AGCT 5 cut(s) 54, 156, 397, 411, 462
Alw21I GWGCWC 1 cut(s) 188
AlwI GGATC 4 cut(s) 121, 121, 134, 142
AlwNI CAGNNNCTG 2 cut(s) 159, 454
ApeKI GCWGC 4 cut(s) 156, 159, 216, 428
ApoI RAATTY 1 cut(s) 388
AspLEI GCGC 1 cut(s) 181
AsuHPI GGTGA 1 cut(s) 311
AsuII TTCGAA 1 cut(s) 346
BamHI GGATCC 1 cut(s) 126
BanI GGYRCC 1 cut(s) 210
BbsI GAAGAC 1 cut(s) 355
Bbv12I GWGCWC 1 cut(s) 188
BbvI GCAGC 4 cut(s) 143, 146, 228, 440
BciT130I CCWGG 2 cut(s) 227, 339
BisI GCNGC 6 cut(s) 6, 157, 160, 214, 217, 429
BlsI GCNGC 6 cut(s) 7, 158, 161, 215, 218, 430
Bme1390I CCNGG 2 cut(s) 227, 339
BmeRI GACNNNNNGTC 1 cut(s) 169
BmiI GGNNCC 2 cut(s) 128, 212
BmrFI CCNGG 2 cut(s) 227, 339
BmrI ACTGGG 1 cut(s) 56
BmuI ACTGGG 1 cut(s) 56
BpiI GAAGAC 1 cut(s) 355
Bpu14I TTCGAA 1 cut(s) 346
BpuEI CTTGAG 1 cut(s) 32
BsaJI CCNNGG 3 cut(s) 225, 252, 338
BsaWI WCCGGW 1 cut(s) 129
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 1 cut(s) 204
Bse118I RCCGGY 1 cut(s) 204
Bse1I ACTGG 1 cut(s) 62
BseBI CCWGG 2 cut(s) 227, 339
BseDI CCNNGG 3 cut(s) 225, 252, 338
BseGI GGATG 2 cut(s) 376, 481
BseLI CCNNNNNNNGG 1 cut(s) 204
BseMII CTCAG 2 cut(s) 287, 461
BseNI ACTGG 1 cut(s) 62
BseRI GAGGAG 7 cut(s) 59, 62, 65, 68, 71, 74, 77
BseXI GCAGC 4 cut(s) 143, 146, 228, 440
BseYI CCCAGC 1 cut(s) 152
BshNI GGYRCC 1 cut(s) 210
BsiHKAI GWGCWC 1 cut(s) 188
BsiSI CCGG 2 cut(s) 130, 205
BslFI GGGAC 1 cut(s) 208
BslI CCNNNNNNNGG 1 cut(s) 204
BsmFI GGGAC 1 cut(s) 208
Bsp119I TTCGAA 1 cut(s) 346
Bsp1286I GDGCHC 1 cut(s) 188
Bsp143I GATC 4 cut(s) 113, 126, 147, 290
BspACI CCGC 4 cut(s) 5, 18, 111, 214
BspCNI CTCAG 2 cut(s) 286, 460
BspLI GGNNCC 2 cut(s) 128, 212
BspPI GGATC 4 cut(s) 121, 121, 134, 142
BspT104I TTCGAA 1 cut(s) 346
BspT107I GGYRCC 1 cut(s) 210
BsrFI RCCGGY 1 cut(s) 204
BsrI ACTGG 1 cut(s) 62
BssAI RCCGGY 1 cut(s) 204
BssECI CCNNGG 3 cut(s) 225, 252, 338
BssMI GATC 4 cut(s) 113, 126, 147, 290
Bst2UI CCWGG 2 cut(s) 227, 339
BstBI TTCGAA 1 cut(s) 346
BstDEI CTNAG 2 cut(s) 273, 447
BstF5I GGATG 2 cut(s) 376, 481
BstHHI GCGC 1 cut(s) 181
BstKTI GATC 4 cut(s) 116, 129, 150, 293
BstMBI GATC 4 cut(s) 113, 126, 147, 290
BstMWI GCNNNNNNNGC 2 cut(s) 210, 437
BstNI CCWGG 2 cut(s) 227, 339
BstNSI RCATGY 2 cut(s) 169, 238
BstSCI CCNGG 2 cut(s) 225, 337
BstV1I GCAGC 4 cut(s) 143, 146, 228, 440
BstV2I GAAGAC 1 cut(s) 355
BstX2I RGATCY 3 cut(s) 113, 126, 147
BstYI RGATCY 3 cut(s) 113, 126, 147
BtsCI GGATG 2 cut(s) 376, 481
BtsI GCAGTG 1 cut(s) 188
BtsIMutI CAGTG 1 cut(s) 188
CaiI CAGNNNCTG 2 cut(s) 159, 454
CfoI GCGC 1 cut(s) 181
Cfr10I RCCGGY 1 cut(s) 204
Csp6I GTAC 1 cut(s) 451
CviAII CATG 2 cut(s) 166, 235
CviJI RGCY 8 cut(s) 54, 156, 257, 343, 397, 411, 462, 478
CviKI_1 RGCY 8 cut(s) 54, 156, 257, 343, 397, 411, 462, 478
CviQI GTAC 1 cut(s) 451
DdeI CTNAG 2 cut(s) 273, 447
DpnI GATC 4 cut(s) 115, 128, 149, 292
DpnII GATC 4 cut(s) 113, 126, 147, 290
DriI GACNNNNNGTC 1 cut(s) 169
Eam1105I GACNNNNNGTC 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 408
EcoRII CCWGG 2 cut(s) 225, 337
FaeI CATG 2 cut(s) 169, 238
FaiI YATR 8 cut(s) 167, 236, 332, 334, 356, 404, 406, 408
FaqI GGGAC 1 cut(s) 208
FatI CATG 2 cut(s) 165, 234
Fnu4HI GCNGC 6 cut(s) 6, 157, 160, 214, 217, 429
FokI GGATG 2 cut(s) 383, 468
Fsp4HI GCNGC 6 cut(s) 6, 157, 160, 214, 217, 429
GlaI GCGC 1 cut(s) 180
GluI GCNGC 6 cut(s) 6, 157, 160, 214, 217, 429
GsaI CCCAGC 1 cut(s) 156
HapII CCGG 2 cut(s) 130, 205
HhaI GCGC 1 cut(s) 181
Hin1II CATG 2 cut(s) 169, 238
Hin6I GCGC 1 cut(s) 179
HinP1I GCGC 1 cut(s) 179
HinfI GANTC 5 cut(s) 121, 142, 221, 302, 377
HpaII CCGG 2 cut(s) 130, 205
HphI GGTGA 1 cut(s) 311
Hpy188I TCNGA 7 cut(s) 126, 147, 175, 253, 276, 288, 307
Hpy188III TCNNGA 1 cut(s) 49
Hpy99I CGWCG 1 cut(s) 175
HpyAV CCTTC 2 cut(s) 112, 293
HpyCH4V TGCA 2 cut(s) 361, 417
HpyF10VI GCNNNNNNNGC 2 cut(s) 210, 437
HpyF3I CTNAG 2 cut(s) 273, 447
Hsp92II CATG 2 cut(s) 169, 238
HspAI GCGC 1 cut(s) 179
Kzo9I GATC 4 cut(s) 113, 126, 147, 290
LmnI GCTCC 1 cut(s) 191
Lsp1109I GCAGC 4 cut(s) 143, 146, 228, 440
MaeIII GTNAC 1 cut(s) 382
MalI GATC 4 cut(s) 115, 128, 149, 292
MboI GATC 4 cut(s) 113, 126, 147, 290
MboII GAAGA 2 cut(s) 325, 360
MflI RGATCY 3 cut(s) 113, 126, 147
MhlI GDGCHC 1 cut(s) 188
MluCI AATT 1 cut(s) 388
MlyI GAGTC 1 cut(s) 230
MmeI TCCRAC 1 cut(s) 153
MseI TTAA 1 cut(s) 138
MspA1I CMGCKG 1 cut(s) 156
MspI CCGG 2 cut(s) 130, 205
MspR9I CCNGG 2 cut(s) 227, 339
MvaI CCWGG 2 cut(s) 227, 339
MwoI GCNNNNNNNGC 2 cut(s) 210, 437
NdeII GATC 4 cut(s) 113, 126, 147, 290
NlaIII CATG 2 cut(s) 169, 238
NlaIV GGNNCC 2 cut(s) 128, 212
NspI RCATGY 2 cut(s) 169, 238
NspV TTCGAA 1 cut(s) 346
PciI ACATGT 2 cut(s) 165, 234
PfeI GAWTC 4 cut(s) 121, 142, 302, 377
PkrI GCNGC 6 cut(s) 7, 158, 161, 215, 218, 430
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
PscI ACATGT 2 cut(s) 165, 234
Psp6I CCWGG 2 cut(s) 225, 337
PspFI CCCAGC 1 cut(s) 152
PspGI CCWGG 2 cut(s) 225, 337
PspN4I GGNNCC 2 cut(s) 128, 212
PstNI CAGNNNCTG 2 cut(s) 159, 454
PsuI RGATCY 3 cut(s) 113, 126, 147
PvuII CAGCTG 1 cut(s) 156
RsaI GTAC 1 cut(s) 452
RsaNI GTAC 1 cut(s) 451
SaqAI TTAA 1 cut(s) 138
SatI GCNGC 6 cut(s) 6, 157, 160, 214, 217, 429
Sau3AI GATC 4 cut(s) 113, 126, 147, 290
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 2 cut(s) 227, 339
SduI GDGCHC 1 cut(s) 188
SfuI TTCGAA 1 cut(s) 346
SmlI CTYRAG 1 cut(s) 47
SmoI CTYRAG 1 cut(s) 47
Sse9I AATT 1 cut(s) 388
SsiI CCGC 4 cut(s) 5, 18, 111, 214
StyD4I CCNGG 2 cut(s) 225, 337
TaqI TCGA 1 cut(s) 346
TasI AATT 1 cut(s) 388
TauI GCSGC 2 cut(s) 8, 216
TfiI GAWTC 4 cut(s) 121, 142, 302, 377
Tru1I TTAA 1 cut(s) 138
Tru9I TTAA 1 cut(s) 138
TscAI CASTG 1 cut(s) 188
TseI GCWGC 4 cut(s) 156, 159, 216, 428
TspGWI ACGGA 1 cut(s) 370
TspRI CASTG 1 cut(s) 188
XapI RAATTY 1 cut(s) 388
XceI RCATGY 2 cut(s) 169, 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.