Rorug03G0201200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0011605
Physical Location & Seq
Reverse (-)
17717994 .. 17722633
4640 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0201200.1

Sequence Viewer

Length: 1518 bp
ATGACTGAGGTACTCCAATCCCCATCTCACTTCCCTTCACCTTCAAGCTCTTCCCCACCCACAAGTAATGATGGCCCATACCCACATGCCCTTATTACCAGTCCTTCCGTAGGTGAGAGCCTGCAAGCCCATTTGAGTTCAGAGGAGGAAGAAGAAGAAGAAGAAGAAGAAGAAGAGAGGAAGAGGAGCGAGAGAGACAGAGAGGGAGATCAGCTGTCGCTCTTGACGCTTCTTGTGGCTGCTTTTAGGAAGTCTTTGATTGGGTGCAGTACTACTGGGACTGGGGCCGAGAGACCTAAGCTTTCCAACATGGAGATTGGGTGGCCTTCCAATGTGAGGCATGTTGCCCATGTCACCTTTGATCGGTTCAATGGCTTTCTGGGTTTGCCGGTTGAGCTTGAGCCTGAGGTTCCCAGGAGGGCTCCTAGTGCTAGTGCGAATGTTTTCGGGGTTTCCACAGAGTCCATGCAGCTATCATTTGATGCTAGAGGAAACAGTGTCCCCACAATACTCATCTTGATGCAGAGACATTTATATGCGCAGGGGGGTCTGCAGGCAGAAGGAATCTTCAGAATTAATGGCGAGAACAGTCAGGAAGAGTACGTCAGGGACCAATTAAACAAGGGAATAATACCAGAGGGTGTTGACATGCACTGTTTAGCAGGTCTTATTAAGGCTTGGTTTAGAGAACTCCCAACTGCTGTATTGGACTCTCTCTCACCAGAGCAGGTAATGCAGTCGCAGACAGAAGAGGAGTGTGCTCAACTTGTTAGGCTCCTTCCGCCAACAGAAGCTGCATTATTGGATTGGGCAGTAAACCTAATGGCCGATGTTGCACAGATGGAACACCTGAACAAGATGAATGCACGCAACATTGCCATGGTCTTTGCGCCAAACATGACTCAAATGGTAGATCCTTTGACTGCATTGATGTATGCAGTCCAAGTGATGAATTTCCTCAAGACTCTTATAGTCAAGACACTAAGAGAGAGAGAAGAGTCTATGGTGGAAACAGCTCCTGTCCCACACCTTGAGCCTTCTGATGAGGATGAGCACCAAAGCACTTTTCAACCATATCTCGAAGAGACTAATAAGGAGGCTAACAAGGAAAATGAAGAGGAGAATGTATTTGTTGCCGAAGAACCTGCTTTACAGAGCCCCCCTCGCACTTTACAAGATGAACCTACAGCTGAAAGTGGATCCCAGAATTTCCTCTCTTCTATCAAATATATGATTCCCGGAGGAAATTGGTCTGTAGTTGATAATTGTCCTTGTGAGGTTGTATCCCAAGTCAGCTCTTTAACAAATGGGCTCCAAGAGGATGGCATAACAGGTCAGGGTAGAGAGGCTCAGACAAACGTTTGGAAGAGTCAACCAAGCGTTTCAAATCTGAAGATGGGATCAAAGGTCACTGAGCAGCTAATGGTTCAAATTGTCGGGCCTGGAGACAAGAGCAAGAGAACTGGGATTCTTAGCCGCATAAATTCGAGGACAGATATCGCTGAAGGTTGGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

505

Amino Acids

55.87

Weight (kDa)

4.68

Isoelectric Point (pI)

65.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 168 - 301 1.6e-22 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 540
Acc36I ACCTGC 3 cut(s) 653, 718, 1153
AciI CCGC 2 cut(s) 782, 1477
AclI AACGTT 1 cut(s) 1359
AclWI GGATC 4 cut(s) 908, 1194, 1207, 1408
AcoI YGGCCR 1 cut(s) 825
AcsI RAATTY 3 cut(s) 952, 1207, 1483
AcuI CTGAAG 2 cut(s) 553, 1412
AfaI GTAC 3 cut(s) 12, 271, 602
AfiI CCNNNNNNNGG 3 cut(s) 110, 336, 363
AgsI TTSAA 5 cut(s) 45, 370, 1070, 1386, 1430
AjnI CCWGG 2 cut(s) 413, 1441
AjuI GAANNNNNNNTTGG 2 cut(s) 1050, 1082
Alw21I GWGCWC 2 cut(s) 763, 1056
Alw26I GTCTC 5 cut(s) 189, 286, 520, 1079, 1440
AlwI GGATC 4 cut(s) 908, 1194, 1207, 1408
AlwNI CAGNNNCTG 2 cut(s) 794, 1019
AoxI GGCC 5 cut(s) 73, 285, 323, 825, 1439
ApeKI GCWGC 4 cut(s) 239, 469, 794, 1417
ApoI RAATTY 3 cut(s) 952, 1207, 1483
AseI ATTAAT 1 cut(s) 576
Asp700I GAANNNNTTC 1 cut(s) 443
AspLEI GCGC 2 cut(s) 541, 892
AspS9I GGNCC 4 cut(s) 74, 285, 610, 1439
AsuC2I CCSGG 1 cut(s) 1239
AsuHPI GGTGA 4 cut(s) 30, 125, 346, 711
AvaII GGWCC 1 cut(s) 610
AxyI CCTNAGG 1 cut(s) 405
BamHI GGATCC 1 cut(s) 1199
BanII GRGCYC 3 cut(s) 424, 1160, 1314
Bbv12I GWGCWC 2 cut(s) 763, 1056
BbvI GCAGC 4 cut(s) 226, 481, 781, 1429
BccI CCATC 5 cut(s) 31, 65, 835, 1316, 1390
BcgI CGANNNNNNTGC 2 cut(s) 1127, 1161
BciT130I CCWGG 2 cut(s) 415, 1443
BciVI GTATCC 1 cut(s) 1294
BcnI CCSGG 1 cut(s) 1239
BcoDI GTCTC 5 cut(s) 189, 286, 520, 1079, 1440
BfaI CTAG 3 cut(s) 426, 432, 486
BfmI CTRYAG 3 cut(s) 551, 1185, 1254
BfuAI ACCTGC 3 cut(s) 653, 718, 1153
BfuI GTATCC 1 cut(s) 1294
BisI GCNGC 5 cut(s) 240, 470, 795, 1418, 1477
BlsI GCNGC 5 cut(s) 241, 471, 796, 1419, 1478
BmcAI AGTACT 1 cut(s) 271
Bme1390I CCNGG 3 cut(s) 415, 1239, 1443
Bme18I GGWCC 1 cut(s) 610
BmgT120I GGNCC 4 cut(s) 74, 285, 610, 1439
BmiI GGNNCC 7 cut(s) 286, 411, 423, 611, 776, 1201, 1313
BmrFI CCNGG 3 cut(s) 415, 1239, 1443
BmrI ACTGGG 3 cut(s) 285, 291, 1473
BmsI GCATC 2 cut(s) 472, 510
BmuI ACTGGG 3 cut(s) 285, 291, 1473
BplI GAGNNNNNCTC 2 cut(s) 1147, 1179
BpmI CTGGAG 1 cut(s) 1464
Bpu10I CCTNAGC 1 cut(s) 297
BpuEI CTTGAG 3 cut(s) 419, 944, 1052
BpuMI CCSGG 1 cut(s) 1239
BsaI GGTCTC 1 cut(s) 286
BsaJI CCNNGG 2 cut(s) 413, 879
BsaXI ACNNNNNCTCC 2 cut(s) 305, 335
Bsc4I CCNNNNNNNGG 3 cut(s) 110, 336, 363
Bse118I RCCGGY 1 cut(s) 388
Bse1I ACTGG 4 cut(s) 99, 280, 286, 1468
Bse21I CCTNAGG 1 cut(s) 405
Bse3DI GCAATG 1 cut(s) 873
BseBI CCWGG 2 cut(s) 415, 1443
BseDI CCNNGG 2 cut(s) 413, 879
BseGI GGATG 2 cut(s) 1054, 1327
BseLI CCNNNNNNNGG 3 cut(s) 110, 336, 363
BseMI GCAATG 1 cut(s) 873
BseMII CTCAG 3 cut(s) 396, 1364, 1404
BseNI ACTGG 4 cut(s) 99, 280, 286, 1468
BseRI GAGGAG 4 cut(s) 158, 199, 767, 1133
BseXI GCAGC 4 cut(s) 226, 481, 781, 1429
BsgI GTGCAG 1 cut(s) 286
BshFI GGCC 5 cut(s) 75, 287, 325, 827, 1441
BsiHKAI GWGCWC 2 cut(s) 763, 1056
BsiSI CCGG 2 cut(s) 389, 1239
BslFI GGGAC 4 cut(s) 292, 485, 623, 1007
BslI CCNNNNNNNGG 3 cut(s) 110, 336, 363
BsmAI GTCTC 5 cut(s) 189, 286, 520, 1079, 1440
BsmFI GGGAC 4 cut(s) 292, 485, 623, 1007
BsmI GAATGC 1 cut(s) 868
BsnI GGCC 5 cut(s) 75, 287, 325, 827, 1441
Bso31I GGTCTC 1 cut(s) 286
Bsp1286I GDGCHC 5 cut(s) 424, 763, 1056, 1160, 1314
Bsp143I GATC 5 cut(s) 208, 361, 913, 1199, 1400
Bsp19I CCATGG 1 cut(s) 879
BspACI CCGC 2 cut(s) 782, 1477
BspANI GGCC 5 cut(s) 75, 287, 325, 827, 1441
BspCNI CTCAG 3 cut(s) 397, 1363, 1405
BspLI GGNNCC 7 cut(s) 286, 411, 423, 611, 776, 1201, 1313
BspMAI CTGCAG 1 cut(s) 555
BspMI ACCTGC 3 cut(s) 653, 718, 1153
BspPI GGATC 4 cut(s) 908, 1194, 1207, 1408
BspQI GCTCTTC 1 cut(s) 55
BspTNI GGTCTC 1 cut(s) 286
BsrDI GCAATG 1 cut(s) 873
BsrFI RCCGGY 1 cut(s) 388
BsrI ACTGG 4 cut(s) 99, 280, 286, 1468
BssAI RCCGGY 1 cut(s) 388
BssECI CCNNGG 2 cut(s) 413, 879
BssMI GATC 5 cut(s) 208, 361, 913, 1199, 1400
BssT1I CCWWGG 1 cut(s) 879
Bst2UI CCWGG 2 cut(s) 415, 1443
Bst4CI ACNGT 3 cut(s) 497, 590, 656
BstAPI GCANNNNNTGC 1 cut(s) 733
BstC8I GCNNGC 4 cut(s) 122, 126, 555, 868
BstDEI CTNAG 7 cut(s) 6, 297, 405, 983, 1350, 1413, 1472
BstDSI CCRYGG 1 cut(s) 879
BstENI CCTNNNNNAGG 1 cut(s) 108
BstF5I GGATG 2 cut(s) 1054, 1327
BstHHI GCGC 2 cut(s) 541, 892
BstKTI GATC 5 cut(s) 211, 364, 916, 1202, 1403
BstMAI GTCTC 5 cut(s) 189, 286, 520, 1079, 1440
BstMBI GATC 5 cut(s) 208, 361, 913, 1199, 1400
BstMWI GCNNNNNNNGC 7 cut(s) 226, 394, 428, 733, 781, 833, 1164
BstNI CCWGG 2 cut(s) 415, 1443
BstNSI RCATGY 3 cut(s) 89, 344, 652
BstSCI CCNGG 3 cut(s) 413, 1237, 1441
BstSFI CTRYAG 3 cut(s) 551, 1185, 1254
BstV1I GCAGC 4 cut(s) 226, 481, 781, 1429
BstX2I RGATCY 2 cut(s) 913, 1199
BstXI CCANNNNNNTGG 1 cut(s) 1322
BstYI RGATCY 2 cut(s) 913, 1199
Bsu36I CCTNAGG 1 cut(s) 405
BsuI GTATCC 1 cut(s) 1294
BsuRI GGCC 5 cut(s) 75, 287, 325, 827, 1441
BtgI CCRYGG 1 cut(s) 879
BtsCI GGATG 2 cut(s) 1054, 1327
BtsIMutI CAGTG 3 cut(s) 502, 652, 1410
BveI ACCTGC 3 cut(s) 653, 718, 1153
Cac8I GCNNGC 4 cut(s) 122, 126, 555, 868
CaiI CAGNNNCTG 2 cut(s) 794, 1019
CfoI GCGC 2 cut(s) 541, 892
Cfr10I RCCGGY 1 cut(s) 388
Cfr13I GGNCC 4 cut(s) 74, 285, 610, 1439
CseI GACGC 1 cut(s) 235
Csp6I GTAC 3 cut(s) 11, 270, 601
CviAII CATG 8 cut(s) 86, 310, 341, 350, 466, 649, 880, 898
CviQI GTAC 3 cut(s) 11, 270, 601
DdeI CTNAG 7 cut(s) 6, 297, 405, 983, 1350, 1413, 1472
DpnI GATC 5 cut(s) 210, 363, 915, 1201, 1402
DpnII GATC 5 cut(s) 208, 361, 913, 1199, 1400
EaeI YGGCCR 1 cut(s) 825
EciI GGCGGA 1 cut(s) 771
Eco130I CCWWGG 1 cut(s) 879
Eco24I GRGCYC 3 cut(s) 424, 1160, 1314
Eco31I GGTCTC 1 cut(s) 286
Eco32I GATATC 1 cut(s) 1498
Eco47I GGWCC 1 cut(s) 610
Eco57I CTGAAG 2 cut(s) 553, 1412
Eco81I CCTNAGG 1 cut(s) 405
EcoNI CCTNNNNNAGG 1 cut(s) 108
EcoRII CCWGG 2 cut(s) 413, 1441
EcoRV GATATC 1 cut(s) 1498
EcoT14I CCWWGG 1 cut(s) 879
EcoT38I GRGCYC 3 cut(s) 424, 1160, 1314
ErhI CCWWGG 1 cut(s) 879
FaeI CATG 8 cut(s) 89, 313, 344, 353, 469, 652, 883, 901
FaqI GGGAC 4 cut(s) 292, 485, 623, 1007
FatI CATG 8 cut(s) 85, 309, 340, 349, 465, 648, 879, 897
Fnu4HI GCNGC 5 cut(s) 240, 470, 795, 1418, 1477
FokI GGATG 2 cut(s) 1061, 1334
FriOI GRGCYC 3 cut(s) 424, 1160, 1314
Fsp4HI GCNGC 5 cut(s) 240, 470, 795, 1418, 1477
FspBI CTAG 3 cut(s) 426, 432, 486
FspI TGCGCA 1 cut(s) 540
GlaI GCGC 2 cut(s) 540, 891
GluI GCNGC 5 cut(s) 240, 470, 795, 1418, 1477
GsuI CTGGAG 1 cut(s) 1464
HaeIII GGCC 5 cut(s) 75, 287, 325, 827, 1441
HapII CCGG 2 cut(s) 389, 1239
HgaI GACGC 1 cut(s) 235
HhaI GCGC 2 cut(s) 541, 892
Hin1II CATG 8 cut(s) 89, 313, 344, 353, 469, 652, 883, 901
Hin6I GCGC 2 cut(s) 539, 890
HinP1I GCGC 2 cut(s) 539, 890
HincII GTYRAC 2 cut(s) 646, 1373
HindII GTYRAC 2 cut(s) 646, 1373
HindIII AAGCTT 1 cut(s) 299
HinfI GANTC 9 cut(s) 461, 564, 710, 901, 964, 998, 1234, 1369, 1468
HpaII CCGG 2 cut(s) 389, 1239
HphI GGTGA 4 cut(s) 30, 125, 346, 711
Hpy166II GTNNAC 3 cut(s) 646, 817, 1373
Hpy188I TCNGA 5 cut(s) 142, 572, 1042, 1353, 1392
Hpy188III TCNNGA 6 cut(s) 223, 517, 593, 961, 976, 1079
Hpy8I GTNNAC 3 cut(s) 646, 817, 1373
HpyAV CCTTC 8 cut(s) 45, 51, 114, 336, 554, 788, 1047, 1499
HpyCH4III ACNGT 3 cut(s) 497, 590, 656
HpyCH4IV ACGT 2 cut(s) 603, 1359
HpyF10VI GCNNNNNNNGC 7 cut(s) 226, 394, 428, 733, 781, 833, 1164
HpyF3I CTNAG 7 cut(s) 6, 297, 405, 983, 1350, 1413, 1472
HpySE526I ACGT 2 cut(s) 603, 1359
Hsp92II CATG 8 cut(s) 89, 313, 344, 353, 469, 652, 883, 901
HspAI GCGC 2 cut(s) 539, 890
Kzo9I GATC 5 cut(s) 208, 361, 913, 1199, 1400
LguI GCTCTTC 1 cut(s) 55
LmnI GCTCC 5 cut(s) 186, 427, 780, 1021, 1317
Lsp1109I GCAGC 4 cut(s) 226, 481, 781, 1429
LweI GCATC 2 cut(s) 472, 510
MaeI CTAG 3 cut(s) 426, 432, 486
MaeII ACGT 2 cut(s) 603, 1359
MaeIII GTNAC 2 cut(s) 352, 1408
MalI GATC 5 cut(s) 210, 363, 915, 1201, 1402
MboI GATC 5 cut(s) 208, 361, 913, 1199, 1400
MflI RGATCY 2 cut(s) 913, 1199
MhlI GDGCHC 5 cut(s) 424, 763, 1056, 1160, 1314
MluCI AATT 8 cut(s) 573, 614, 952, 1207, 1246, 1264, 1431, 1483
MlyI GAGTC 6 cut(s) 470, 704, 895, 958, 1007, 1378
MmeI TCCRAC 1 cut(s) 330
MroXI GAANNNNTTC 1 cut(s) 443
MseI TTAA 4 cut(s) 576, 617, 672, 1301
MslI CAYNNNNRTG 3 cut(s) 518, 534, 878
MspA1I CMGCKG 2 cut(s) 214, 1190
MspI CCGG 2 cut(s) 389, 1239
MspR9I CCNGG 3 cut(s) 415, 1239, 1443
Mva1269I GAATGC 1 cut(s) 868
MvaI CCWGG 2 cut(s) 415, 1443
MwoI GCNNNNNNNGC 7 cut(s) 226, 394, 428, 733, 781, 833, 1164
NciI CCSGG 1 cut(s) 1239
NcoI CCATGG 1 cut(s) 879
NdeII GATC 5 cut(s) 208, 361, 913, 1199, 1400
NlaIII CATG 8 cut(s) 89, 313, 344, 353, 469, 652, 883, 901
NlaIV GGNNCC 7 cut(s) 286, 411, 423, 611, 776, 1201, 1313
NmeAIII GCCGAG 1 cut(s) 313
NmuCI GTSAC 2 cut(s) 352, 1408
NsbI TGCGCA 1 cut(s) 540
NspI RCATGY 3 cut(s) 89, 344, 652
PciSI GCTCTTC 1 cut(s) 55
PctI GAATGC 1 cut(s) 868
PdmI GAANNNNTTC 1 cut(s) 443
PfeI GAWTC 3 cut(s) 564, 1234, 1468
PfoI TCCNGGA 1 cut(s) 1237
PkrI GCNGC 5 cut(s) 241, 471, 796, 1419, 1478
PleI GAGTC 6 cut(s) 469, 704, 895, 958, 1006, 1377
PpsI GAGTC 6 cut(s) 469, 704, 895, 958, 1006, 1377
PshBI ATTAAT 1 cut(s) 576
Psp1406I AACGTT 1 cut(s) 1359
Psp6I CCWGG 2 cut(s) 413, 1441
PspGI CCWGG 2 cut(s) 413, 1441
PspN4I GGNNCC 7 cut(s) 286, 411, 423, 611, 776, 1201, 1313
PspPI GGNCC 4 cut(s) 74, 285, 610, 1439
PsrI GAACNNNNNNTAC 2 cut(s) 1134, 1166
PstI CTGCAG 1 cut(s) 555
PstNI CAGNNNCTG 2 cut(s) 794, 1019
PsuI RGATCY 2 cut(s) 913, 1199
PvuII CAGCTG 2 cut(s) 214, 1190
RsaI GTAC 3 cut(s) 12, 271, 602
RsaNI GTAC 3 cut(s) 11, 270, 601
RseI CAYNNNNRTG 3 cut(s) 518, 534, 878
SapI GCTCTTC 1 cut(s) 55
SaqAI TTAA 4 cut(s) 576, 617, 672, 1301
SatI GCNGC 5 cut(s) 240, 470, 795, 1418, 1477
Sau3AI GATC 5 cut(s) 208, 361, 913, 1199, 1400
Sau96I GGNCC 4 cut(s) 74, 285, 610, 1439
ScaI AGTACT 1 cut(s) 271
SchI GAGTC 6 cut(s) 470, 704, 895, 958, 1007, 1378
ScrFI CCNGG 3 cut(s) 415, 1239, 1443
SduI GDGCHC 5 cut(s) 424, 763, 1056, 1160, 1314
SfaNI GCATC 2 cut(s) 472, 510
SfcI CTRYAG 3 cut(s) 551, 1185, 1254
SinI GGWCC 1 cut(s) 610
SmiMI CAYNNNNRTG 3 cut(s) 518, 534, 878
SmlI CTYRAG 3 cut(s) 398, 959, 1031
SmoI CTYRAG 3 cut(s) 398, 959, 1031
Sse9I AATT 8 cut(s) 573, 614, 952, 1207, 1246, 1264, 1431, 1483
SsiI CCGC 2 cut(s) 782, 1477
SspMI CTAG 3 cut(s) 426, 432, 486
StyD4I CCNGG 3 cut(s) 413, 1237, 1441
StyI CCWWGG 1 cut(s) 879
TaaI ACNGT 3 cut(s) 497, 590, 656
TaiI ACGT 2 cut(s) 606, 1362
TaqI TCGA 2 cut(s) 1080, 1487
TasI AATT 8 cut(s) 573, 614, 952, 1207, 1246, 1264, 1431, 1483
TatI WGTACW 1 cut(s) 269
TauI GCSGC 1 cut(s) 1479
TfiI GAWTC 3 cut(s) 564, 1234, 1468
Tru1I TTAA 4 cut(s) 576, 617, 672, 1301
Tru9I TTAA 4 cut(s) 576, 617, 672, 1301
TscAI CASTG 3 cut(s) 502, 659, 1417
TseFI GTSAC 2 cut(s) 352, 1408
TseI GCWGC 4 cut(s) 239, 469, 794, 1417
Tsp45I GTSAC 2 cut(s) 352, 1408
TspDTI ATGAA 4 cut(s) 875, 965, 1128, 1194
TspGWI ACGGA 1 cut(s) 97
TspRI CASTG 3 cut(s) 502, 659, 1417
VpaK11BI GGWCC 1 cut(s) 610
VspI ATTAAT 1 cut(s) 576
XagI CCTNNNNNAGG 1 cut(s) 108
XapI RAATTY 3 cut(s) 952, 1207, 1483
XceI RCATGY 3 cut(s) 89, 344, 652
XmnI GAANNNNTTC 1 cut(s) 443
XspI CTAG 3 cut(s) 426, 432, 486
ZrmI AGTACT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.