RLG00000002511

Male gamete fusion factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006978
Physical Location & Seq
Forward (+)
35484614 .. 35487177
2564 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002511

Sequence Viewer

Length: 591 bp
ATGTCTCTCTTTACTTCATTAGCTTGCCGGCTTCACTTCCATGGCTTCTCCTCCTTCCTCTACTCAAACCCTCAGCCACAGATCTCATTTCAAGCCAATCGAACGATTGGTGGTCCAGGATCACAAAGGCATGGGACACCTAGGAGGAATGCCACAACAGGTCCAGAAGCACAGACACAGAGCAAGAAGACACGTAACTGGCCAAGTACTGTAGCTCTTAGAGAGATTCATCACTTTCAGAAGTCGGAGAAGCTGGTTGTCCCAAGTGCACCTTTCATCAGAGTTCCAATGTGCTGTCCTTGTGGGCCTCAGCGGCAGGTACCTTCATCATGTGGAAATGTTTTTGACAAGTTGGTGAAAGAAAAACCCAATACAGCTCATTGTGTCCGCTTTCCAGTTGATTGCCTATGGATTCTGCCATTCTTATCTCCTTGTTTTGAAGGACAAAAAGGTCACAGAAAGATTTTTAAGGCTCCCTCACTGCCCATTTGCCACTCCCAAAGAAGAGCACTCGAAAGTTTCCAGAATTTCGTGCGAGTCTGGTTTAATTCATATGGATCATCTGCTGTTAGTTCTGGAGCACTCCTCTAG

Protein Analysis

197

Amino Acids

21.93

Weight (kDa)

10.08

Isoelectric Point (pI)

53.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAP2-GCS1 PF10699 96 - 132 1.1e-07 Male gamete fusion factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 450
Acc36I ACCTGC 1 cut(s) 307
Acc65I GGTACC 1 cut(s) 319
AccB1I GGYRCC 1 cut(s) 319
AciI CCGC 2 cut(s) 313, 388
AclWI GGATC 2 cut(s) 127, 565
AcoI YGGCCR 1 cut(s) 200
AcsI RAATTY 1 cut(s) 526
AfaI GTAC 2 cut(s) 208, 321
AflIII ACRYGT 1 cut(s) 191
AgsI TTSAA 2 cut(s) 92, 440
AjnI CCWGG 1 cut(s) 115
AluBI AGCT 4 cut(s) 23, 215, 253, 377
AluI AGCT 4 cut(s) 23, 215, 253, 377
Alw21I GWGCWC 3 cut(s) 271, 511, 583
Alw26I GTCTC 1 cut(s) 9
Alw44I GTGCAC 1 cut(s) 267
AlwI GGATC 2 cut(s) 127, 565
AoxI GGCC 2 cut(s) 200, 305
ApaLI GTGCAC 1 cut(s) 267
ApoI RAATTY 1 cut(s) 526
Asp718I GGTACC 1 cut(s) 319
AspA2I CCTAGG 1 cut(s) 140
AspS9I GGNCC 3 cut(s) 113, 161, 305
AsuHPI GGTGA 1 cut(s) 367
AvaII GGWCC 2 cut(s) 113, 161
AvrII CCTAGG 1 cut(s) 140
BaeGI GKGCMC 1 cut(s) 271
BalI TGGCCA 1 cut(s) 202
BanI GGYRCC 1 cut(s) 319
BbsI GAAGAC 1 cut(s) 194
Bbv12I GWGCWC 3 cut(s) 271, 511, 583
BbvCI CCTCAGC 2 cut(s) 72, 309
BciT130I CCWGG 1 cut(s) 117
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 2 cut(s) 141, 589
BfmI CTRYAG 1 cut(s) 210
BfuAI ACCTGC 1 cut(s) 307
BglI GCCNNNNNGGC 1 cut(s) 313
BglII AGATCT 1 cut(s) 81
BisI GCNGC 1 cut(s) 314
BlnI CCTAGG 1 cut(s) 140
BlsI GCNGC 1 cut(s) 315
BmcAI AGTACT 1 cut(s) 208
Bme1390I CCNGG 1 cut(s) 117
Bme18I GGWCC 2 cut(s) 113, 161
BmgT120I GGNCC 3 cut(s) 113, 161, 305
BmiI GGNNCC 2 cut(s) 321, 474
BmrFI CCNGG 1 cut(s) 117
BpiI GAAGAC 1 cut(s) 194
BplI GAGNNNNNCTC 1 cut(s) 570
Bpu10I CCTNAGC 2 cut(s) 72, 309
BsaAI YACGTR 1 cut(s) 194
BsaJI CCNNGG 2 cut(s) 40, 140
Bse118I RCCGGY 1 cut(s) 27
Bse1I ACTGG 2 cut(s) 203, 395
BseBI CCWGG 1 cut(s) 117
BseDI CCNNGG 2 cut(s) 40, 140
BseMII CTCAG 2 cut(s) 86, 323
BseNI ACTGG 2 cut(s) 203, 395
BseRI GAGGAG 2 cut(s) 40, 575
BseSI GKGCMC 1 cut(s) 271
BshFI GGCC 2 cut(s) 202, 307
BshNI GGYRCC 1 cut(s) 319
BsiHKAI GWGCWC 3 cut(s) 271, 511, 583
BsiSI CCGG 1 cut(s) 28
BslFI GGGAC 2 cut(s) 148, 245
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 2 cut(s) 148, 245
BsmI GAATGC 1 cut(s) 154
BsnI GGCC 2 cut(s) 202, 307
Bsp1286I GDGCHC 3 cut(s) 271, 511, 583
Bsp143I GATC 3 cut(s) 81, 119, 557
Bsp19I CCATGG 1 cut(s) 40
BspACI CCGC 2 cut(s) 313, 388
BspANI GGCC 2 cut(s) 202, 307
BspCNI CTCAG 2 cut(s) 85, 322
BspLI GGNNCC 2 cut(s) 321, 474
BspMI ACCTGC 1 cut(s) 307
BspPI GGATC 2 cut(s) 127, 565
BspQI GCTCTTC 1 cut(s) 499
BspT107I GGYRCC 1 cut(s) 319
BsrFI RCCGGY 1 cut(s) 27
BsrI ACTGG 2 cut(s) 203, 395
BssAI RCCGGY 1 cut(s) 27
BssECI CCNNGG 2 cut(s) 40, 140
BssMI GATC 3 cut(s) 81, 119, 557
BssT1I CCWWGG 2 cut(s) 40, 140
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 1 cut(s) 211
Bst6I CTCTTC 1 cut(s) 499
BstBAI YACGTR 1 cut(s) 194
BstC8I GCNNGC 2 cut(s) 25, 29
BstDEI CTNAG 3 cut(s) 72, 218, 309
BstDSI CCRYGG 1 cut(s) 40
BstKTI GATC 3 cut(s) 84, 122, 560
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 3 cut(s) 81, 119, 557
BstMWI GCNNNNNNNGC 1 cut(s) 313
BstNI CCWGG 1 cut(s) 117
BstSCI CCNGG 1 cut(s) 115
BstSFI CTRYAG 1 cut(s) 210
BstSLI GKGCMC 1 cut(s) 271
BstV2I GAAGAC 1 cut(s) 194
BstX2I RGATCY 1 cut(s) 81
BstYI RGATCY 1 cut(s) 81
BsuRI GGCC 2 cut(s) 202, 307
BtgI CCRYGG 1 cut(s) 40
BtsI GCAGTG 1 cut(s) 479
BtsIMutI CAGTG 1 cut(s) 479
BveI ACCTGC 1 cut(s) 307
Cac8I GCNNGC 2 cut(s) 25, 29
Cfr10I RCCGGY 1 cut(s) 27
Cfr13I GGNCC 3 cut(s) 113, 161, 305
Csp6I GTAC 2 cut(s) 207, 320
CviAII CATG 3 cut(s) 41, 131, 330
CviQI GTAC 2 cut(s) 207, 320
DdeI CTNAG 3 cut(s) 72, 218, 309
DpnI GATC 3 cut(s) 83, 121, 559
DpnII GATC 3 cut(s) 81, 119, 557
DrdI GACNNNNNNGTC 1 cut(s) 450
DseDI GACNNNNNNGTC 1 cut(s) 450
EaeI YGGCCR 1 cut(s) 200
Eam1104I CTCTTC 1 cut(s) 499
EarI CTCTTC 1 cut(s) 499
Eco130I CCWWGG 2 cut(s) 40, 140
Eco47I GGWCC 2 cut(s) 113, 161
EcoRII CCWGG 1 cut(s) 115
EcoT14I CCWWGG 2 cut(s) 40, 140
ErhI CCWWGG 2 cut(s) 40, 140
FaeI CATG 3 cut(s) 44, 134, 333
FaiI YATR 6 cut(s) 42, 132, 331, 409, 553, 555
FalI AAGNNNNNCTT 2 cut(s) 256, 288
FaqI GGGAC 2 cut(s) 148, 245
FatI CATG 3 cut(s) 40, 130, 329
FauNDI CATATG 1 cut(s) 553
Fnu4HI GCNGC 1 cut(s) 314
Fsp4HI GCNGC 1 cut(s) 314
FspBI CTAG 2 cut(s) 141, 589
GluI GCNGC 1 cut(s) 314
HaeIII GGCC 2 cut(s) 202, 307
HapII CCGG 1 cut(s) 28
Hin1II CATG 3 cut(s) 44, 134, 333
HinfI GANTC 3 cut(s) 226, 412, 537
HpaII CCGG 1 cut(s) 28
HphI GGTGA 1 cut(s) 367
Hpy166II GTNNAC 1 cut(s) 269
Hpy188I TCNGA 3 cut(s) 240, 247, 281
Hpy188III TCNNGA 3 cut(s) 164, 523, 576
Hpy8I GTNNAC 1 cut(s) 269
HpyAV CCTTC 3 cut(s) 64, 333, 434
HpyCH4III ACNGT 1 cut(s) 211
HpyCH4IV ACGT 1 cut(s) 193
HpyCH4V TGCA 1 cut(s) 269
HpyF10VI GCNNNNNNNGC 1 cut(s) 313
HpyF3I CTNAG 3 cut(s) 72, 218, 309
HpySE526I ACGT 1 cut(s) 193
Hsp92II CATG 3 cut(s) 44, 134, 333
KpnI GGTACC 1 cut(s) 323
KroI GCCGGC 1 cut(s) 27
KroNI GCCGGC 1 cut(s) 29
Kzo9I GATC 3 cut(s) 81, 119, 557
LguI GCTCTTC 1 cut(s) 499
LmnI GCTCC 2 cut(s) 478, 578
MaeI CTAG 2 cut(s) 141, 589
MaeII ACGT 1 cut(s) 193
MaeIII GTNAC 2 cut(s) 194, 452
MalI GATC 3 cut(s) 83, 121, 559
MboI GATC 3 cut(s) 81, 119, 557
MboII GAAGA 2 cut(s) 199, 516
MflI RGATCY 1 cut(s) 81
MhlI GDGCHC 3 cut(s) 271, 511, 583
MlsI TGGCCA 1 cut(s) 202
MluCI AATT 2 cut(s) 526, 547
MluNI TGGCCA 1 cut(s) 202
MlyI GAGTC 1 cut(s) 546
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 6 cut(s) 61, 68, 81, 138, 318, 487
Mox20I TGGCCA 1 cut(s) 202
MroNI GCCGGC 1 cut(s) 27
MscI TGGCCA 1 cut(s) 202
MseI TTAA 2 cut(s) 468, 546
MslI CAYNNNNRTG 1 cut(s) 39
Msp20I TGGCCA 1 cut(s) 202
MspA1I CMGCKG 1 cut(s) 313
MspI CCGG 1 cut(s) 28
MspR9I CCNGG 1 cut(s) 117
Mva1269I GAATGC 1 cut(s) 154
MvaI CCWGG 1 cut(s) 117
MwoI GCNNNNNNNGC 1 cut(s) 313
NaeI GCCGGC 1 cut(s) 29
NcoI CCATGG 1 cut(s) 40
NdeI CATATG 1 cut(s) 553
NdeII GATC 3 cut(s) 81, 119, 557
NgoMIV GCCGGC 1 cut(s) 27
NlaIII CATG 3 cut(s) 44, 134, 333
NlaIV GGNNCC 2 cut(s) 321, 474
NmuCI GTSAC 1 cut(s) 452
PciSI GCTCTTC 1 cut(s) 499
PctI GAATGC 1 cut(s) 154
PdiI GCCGGC 1 cut(s) 29
PfeI GAWTC 2 cut(s) 226, 412
PfoI TCCNGGA 1 cut(s) 115
PkrI GCNGC 1 cut(s) 315
PleI GAGTC 1 cut(s) 545
PpsI GAGTC 1 cut(s) 545
Ppu21I YACGTR 1 cut(s) 194
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 2 cut(s) 321, 474
PspPI GGNCC 3 cut(s) 113, 161, 305
PsuI RGATCY 1 cut(s) 81
RsaI GTAC 2 cut(s) 208, 321
RsaNI GTAC 2 cut(s) 207, 320
RseI CAYNNNNRTG 1 cut(s) 39
SapI GCTCTTC 1 cut(s) 499
SaqAI TTAA 2 cut(s) 468, 546
SatI GCNGC 1 cut(s) 314
Sau3AI GATC 3 cut(s) 81, 119, 557
Sau96I GGNCC 3 cut(s) 113, 161, 305
ScaI AGTACT 1 cut(s) 208
SchI GAGTC 1 cut(s) 546
ScrFI CCNGG 1 cut(s) 117
SduI GDGCHC 3 cut(s) 271, 511, 583
SfcI CTRYAG 1 cut(s) 210
SinI GGWCC 2 cut(s) 113, 161
SmiMI CAYNNNNRTG 1 cut(s) 39
Sse9I AATT 2 cut(s) 526, 547
SsiI CCGC 2 cut(s) 313, 388
SspMI CTAG 2 cut(s) 141, 589
StyD4I CCNGG 1 cut(s) 115
StyI CCWWGG 2 cut(s) 40, 140
TaaI ACNGT 1 cut(s) 211
TaiI ACGT 1 cut(s) 196
TaqI TCGA 2 cut(s) 100, 513
TasI AATT 2 cut(s) 526, 547
TatI WGTACW 1 cut(s) 206
TauI GCSGC 1 cut(s) 316
TfiI GAWTC 2 cut(s) 226, 412
Tru1I TTAA 2 cut(s) 468, 546
Tru9I TTAA 2 cut(s) 468, 546
TscAI CASTG 1 cut(s) 486
TseFI GTSAC 1 cut(s) 452
Tsp45I GTSAC 1 cut(s) 452
TspDTI ATGAA 5 cut(s) 6, 218, 265, 315, 540
TspRI CASTG 1 cut(s) 486
VneI GTGCAC 1 cut(s) 267
VpaK11BI GGWCC 2 cut(s) 113, 161
XapI RAATTY 1 cut(s) 526
XmaJI CCTAGG 1 cut(s) 140
XspI CTAG 2 cut(s) 141, 589
ZrmI AGTACT 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.