Rh1DG277500

Histone H3-like centromeric protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0006978
Physical Location & Seq
Reverse (-)
49903401 .. 49907619
4219 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG277500.1

Sequence Viewer

Length: 507 bp
ATGGCGAGGGTCAAGCACACAGCTGCCAGGAAAGACCGGATTAAAACCGCTCGCCGGCAACCAGGAGGCTCGCCGGCGCCGGCGCCGTCACCGGGACCTTCTGGAAGGCCGAGGAAAAGTCCAAGGAAAAGTCCGACAAGTCCAGGATCACAGAGGCAGGGGACACCTAGGAGGAATGCCACAACAGGTCCAGAAGCACAGACACAGAGGAAGAAGAGACGTAACAGGCCAGGAACTGTAGCTCTTCGGGAGATTCGTCACTTTCAGAAGTCGGTGAAGCTGGTTATCCCAAGTGCACCTTTCATCAGAGCTGTCAGAGAGATCACTGGTACCTTGTCTAATGATGTTACGCGTTGGACTGCTGAAGCTTTACAGTGTATTCATGAGGCAGCAGAGGATTATCTAGTTCATTTGTTTGAAGATTCAATGCTCTGTGCAATTCATGCAAAACGTGTTACTCTTAGTAAGTCAGACTGGACATTTAATATCTGCTTACTAATTTTTTAG

Protein Analysis

168

Amino Acids

18.74

Weight (kDa)

11.43

Isoelectric Point (pI)

67.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 77 - 161 1.2e-22 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 329
AccB1I GGYRCC 3 cut(s) 76, 82, 329
AccBSI CCGCTC 1 cut(s) 50
AccII CGCG 1 cut(s) 352
AciI CCGC 1 cut(s) 48
AclWI GGATC 1 cut(s) 154
AcuI CTGAAG 1 cut(s) 384
AcyI GRCGYC 2 cut(s) 77, 83
AfaI GTAC 1 cut(s) 331
AfiI CCNNNNNNNGG 2 cut(s) 54, 92
AflIII ACRYGT 2 cut(s) 350, 451
AgsI TTSAA 2 cut(s) 419, 426
AjnI CCWGG 4 cut(s) 26, 61, 142, 229
AluBI AGCT 5 cut(s) 23, 242, 280, 311, 368
AluI AGCT 5 cut(s) 23, 242, 280, 311, 368
Alw21I GWGCWC 1 cut(s) 298
Alw26I GTCTC 1 cut(s) 211
Alw44I GTGCAC 1 cut(s) 294
AlwI GGATC 1 cut(s) 154
AlwNI CAGNNNCTG 1 cut(s) 236
AoxI GGCC 2 cut(s) 107, 227
ApaLI GTGCAC 1 cut(s) 294
ApeKI GCWGC 2 cut(s) 23, 389
Asp718I GGTACC 1 cut(s) 329
AspA2I CCTAGG 1 cut(s) 167
AspLEI GCGC 2 cut(s) 79, 85
AspS9I GGNCC 2 cut(s) 95, 188
AsuC2I CCSGG 1 cut(s) 93
AsuHPI GGTGA 2 cut(s) 81, 286
AvaII GGWCC 2 cut(s) 95, 188
AvrII CCTAGG 1 cut(s) 167
BaeGI GKGCMC 1 cut(s) 298
BanI GGYRCC 3 cut(s) 76, 82, 329
Bbv12I GWGCWC 1 cut(s) 298
BbvI GCAGC 2 cut(s) 10, 401
BceAI ACGGC 1 cut(s) 70
BciT130I CCWGG 4 cut(s) 28, 63, 144, 231
BcnI CCSGG 1 cut(s) 93
BcoDI GTCTC 1 cut(s) 211
BfaI CTAG 2 cut(s) 168, 404
BfmI CTRYAG 1 cut(s) 237
BfoI RGCGCY 2 cut(s) 80, 86
BisI GCNGC 2 cut(s) 24, 390
BlnI CCTAGG 1 cut(s) 167
BlsI GCNGC 2 cut(s) 25, 391
Bme1390I CCNGG 5 cut(s) 28, 63, 93, 144, 231
Bme18I GGWCC 2 cut(s) 95, 188
BmgT120I GGNCC 2 cut(s) 95, 188
BmiI GGNNCC 4 cut(s) 78, 84, 96, 331
BmrFI CCNGG 5 cut(s) 28, 63, 93, 144, 231
BpuMI CCSGG 1 cut(s) 93
BsaHI GRCGYC 2 cut(s) 77, 83
BsaJI CCNNGG 3 cut(s) 110, 122, 167
BsaWI WCCGGW 1 cut(s) 36
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 92
Bse118I RCCGGY 3 cut(s) 54, 73, 79
Bse1I ACTGG 2 cut(s) 331, 479
BseBI CCWGG 4 cut(s) 28, 63, 144, 231
BseDI CCNNGG 3 cut(s) 110, 122, 167
BseLI CCNNNNNNNGG 2 cut(s) 54, 92
BseNI ACTGG 2 cut(s) 331, 479
BseSI GKGCMC 1 cut(s) 298
BseXI GCAGC 2 cut(s) 10, 401
Bsh1236I CGCG 1 cut(s) 352
BshFI GGCC 2 cut(s) 109, 229
BshNI GGYRCC 3 cut(s) 76, 82, 329
BsiHKAI GWGCWC 1 cut(s) 298
BsiSI CCGG 5 cut(s) 37, 55, 74, 80, 92
BslFI GGGAC 2 cut(s) 108, 175
BslI CCNNNNNNNGG 2 cut(s) 54, 92
BsmAI GTCTC 1 cut(s) 211
BsmBI CGTCTC 1 cut(s) 211
BsmFI GGGAC 2 cut(s) 108, 175
BsmI GAATGC 1 cut(s) 181
BsnI GGCC 2 cut(s) 109, 229
Bsp1286I GDGCHC 1 cut(s) 298
Bsp143I GATC 2 cut(s) 146, 321
BspACI CCGC 1 cut(s) 48
BspANI GGCC 2 cut(s) 109, 229
BspFNI CGCG 1 cut(s) 352
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 4 cut(s) 78, 84, 96, 331
BspPI GGATC 1 cut(s) 154
BspQI GCTCTTC 1 cut(s) 249
BspT107I GGYRCC 3 cut(s) 76, 82, 329
BsrBI CCGCTC 1 cut(s) 50
BsrFI RCCGGY 3 cut(s) 54, 73, 79
BsrI ACTGG 2 cut(s) 331, 479
BssAI RCCGGY 3 cut(s) 54, 73, 79
BssECI CCNNGG 3 cut(s) 110, 122, 167
BssMI GATC 2 cut(s) 146, 321
BssNI GRCGYC 2 cut(s) 77, 83
BssT1I CCWWGG 2 cut(s) 122, 167
Bst2UI CCWGG 4 cut(s) 28, 63, 144, 231
Bst4CI ACNGT 2 cut(s) 238, 375
Bst6I CTCTTC 2 cut(s) 209, 249
BstACI GRCGYC 2 cut(s) 77, 83
BstAPI GCANNNNNTGC 1 cut(s) 443
BstC8I GCNNGC 5 cut(s) 52, 56, 71, 75, 81
BstDEI CTNAG 1 cut(s) 461
BstFNI CGCG 1 cut(s) 352
BstH2I RGCGCY 2 cut(s) 80, 86
BstHHI GCGC 2 cut(s) 79, 85
BstKTI GATC 2 cut(s) 149, 324
BstMAI GTCTC 1 cut(s) 211
BstMBI GATC 2 cut(s) 146, 321
BstMWI GCNNNNNNNGC 1 cut(s) 443
BstNI CCWGG 4 cut(s) 28, 63, 144, 231
BstSCI CCNGG 5 cut(s) 26, 61, 91, 142, 229
BstSFI CTRYAG 1 cut(s) 237
BstSLI GKGCMC 1 cut(s) 298
BstUI CGCG 1 cut(s) 352
BstV1I GCAGC 2 cut(s) 10, 401
BsuRI GGCC 2 cut(s) 109, 229
BtsIMutI CAGTG 2 cut(s) 324, 380
Cac8I GCNNGC 5 cut(s) 52, 56, 71, 75, 81
CaiI CAGNNNCTG 1 cut(s) 236
CciI TCATGA 1 cut(s) 382
CfoI GCGC 2 cut(s) 79, 85
Cfr10I RCCGGY 3 cut(s) 54, 73, 79
Cfr13I GGNCC 2 cut(s) 95, 188
Csp6I GTAC 1 cut(s) 330
CviAII CATG 2 cut(s) 383, 443
CviJI RGCY 8 cut(s) 23, 69, 109, 229, 242, 280, 311, 368
CviKI_1 RGCY 8 cut(s) 23, 69, 109, 229, 242, 280, 311, 368
CviQI GTAC 1 cut(s) 330
DdeI CTNAG 1 cut(s) 461
DinI GGCGCC 2 cut(s) 78, 84
DpnI GATC 2 cut(s) 148, 323
DpnII GATC 2 cut(s) 146, 321
Eam1104I CTCTTC 2 cut(s) 209, 249
EarI CTCTTC 2 cut(s) 209, 249
Eco130I CCWWGG 2 cut(s) 122, 167
Eco47I GGWCC 2 cut(s) 95, 188
Eco57I CTGAAG 1 cut(s) 384
EcoO109I RGGNCCY 1 cut(s) 95
EcoRII CCWGG 4 cut(s) 26, 61, 142, 229
EcoT14I CCWWGG 2 cut(s) 122, 167
EgeI GGCGCC 2 cut(s) 78, 84
EheI GGCGCC 2 cut(s) 78, 84
ErhI CCWWGG 2 cut(s) 122, 167
Esp3I CGTCTC 1 cut(s) 211
FaeI CATG 2 cut(s) 386, 446
FaiI YATR 2 cut(s) 384, 444
FalI AAGNNNNNCTT 2 cut(s) 283, 315
FaqI GGGAC 2 cut(s) 108, 175
FatI CATG 2 cut(s) 382, 442
Fnu4HI GCNGC 2 cut(s) 24, 390
Fsp4HI GCNGC 2 cut(s) 24, 390
FspBI CTAG 2 cut(s) 168, 404
GlaI GCGC 2 cut(s) 78, 84
GluI GCNGC 2 cut(s) 24, 390
HaeII RGCGCY 2 cut(s) 80, 86
HaeIII GGCC 2 cut(s) 109, 229
HapII CCGG 5 cut(s) 37, 55, 74, 80, 92
HhaI GCGC 2 cut(s) 79, 85
Hin1I GRCGYC 2 cut(s) 77, 83
Hin1II CATG 2 cut(s) 386, 446
Hin6I GCGC 2 cut(s) 77, 83
HinP1I GCGC 2 cut(s) 77, 83
HindIII AAGCTT 1 cut(s) 366
HinfI GANTC 2 cut(s) 253, 422
HpaII CCGG 5 cut(s) 37, 55, 74, 80, 92
HphI GGTGA 2 cut(s) 81, 286
Hpy166II GTNNAC 1 cut(s) 296
Hpy188I TCNGA 5 cut(s) 135, 267, 308, 317, 472
Hpy188III TCNNGA 4 cut(s) 102, 191, 248, 383
Hpy8I GTNNAC 1 cut(s) 296
HpyAV CCTTC 2 cut(s) 99, 108
HpyCH4III ACNGT 2 cut(s) 238, 375
HpyCH4IV ACGT 2 cut(s) 220, 451
HpyCH4V TGCA 3 cut(s) 296, 437, 446
HpyF10VI GCNNNNNNNGC 1 cut(s) 443
HpyF3I CTNAG 1 cut(s) 461
HpySE526I ACGT 2 cut(s) 220, 451
Hsp92I GRCGYC 2 cut(s) 77, 83
Hsp92II CATG 2 cut(s) 386, 446
HspAI GCGC 2 cut(s) 77, 83
KasI GGCGCC 2 cut(s) 76, 82
KpnI GGTACC 1 cut(s) 333
KroI GCCGGC 3 cut(s) 54, 73, 79
KroNI GCCGGC 3 cut(s) 56, 75, 81
Kzo9I GATC 2 cut(s) 146, 321
LguI GCTCTTC 1 cut(s) 249
Lsp1109I GCAGC 2 cut(s) 10, 401
MaeI CTAG 2 cut(s) 168, 404
MaeII ACGT 2 cut(s) 220, 451
MaeIII GTNAC 5 cut(s) 87, 221, 257, 346, 454
MalI GATC 2 cut(s) 148, 323
MbiI CCGCTC 1 cut(s) 50
MboI GATC 2 cut(s) 146, 321
MboII GAAGA 4 cut(s) 223, 226, 236, 431
MhlI GDGCHC 1 cut(s) 298
MluCI AATT 2 cut(s) 438, 498
MluI ACGCGT 1 cut(s) 350
Mly113I GGCGCC 2 cut(s) 77, 83
MmeI TCCRAC 2 cut(s) 158, 335
MnlI CCTC 7 cut(s) 59, 105, 147, 165, 201, 379, 388
MreI CGCCGGCG 2 cut(s) 73, 79
MroNI GCCGGC 3 cut(s) 54, 73, 79
MseI TTAA 2 cut(s) 42, 483
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 5 cut(s) 37, 55, 74, 80, 92
MspR9I CCNGG 5 cut(s) 28, 63, 93, 144, 231
Mva1269I GAATGC 1 cut(s) 181
MvaI CCWGG 4 cut(s) 28, 63, 144, 231
MvnI CGCG 1 cut(s) 352
MwoI GCNNNNNNNGC 1 cut(s) 443
NaeI GCCGGC 3 cut(s) 56, 75, 81
NarI GGCGCC 2 cut(s) 77, 83
NciI CCSGG 1 cut(s) 93
NdeII GATC 2 cut(s) 146, 321
NgoMIV GCCGGC 3 cut(s) 54, 73, 79
NlaIII CATG 2 cut(s) 386, 446
NlaIV GGNNCC 4 cut(s) 78, 84, 96, 331
NmeAIII GCCGAG 1 cut(s) 135
NmuCI GTSAC 2 cut(s) 87, 257
PagI TCATGA 1 cut(s) 382
PciSI GCTCTTC 1 cut(s) 249
PcsI WCGNNNNNNNCGW 1 cut(s) 253
PctI GAATGC 1 cut(s) 181
PdiI GCCGGC 3 cut(s) 56, 75, 81
PfeI GAWTC 2 cut(s) 253, 422
PfoI TCCNGGA 1 cut(s) 142
PkrI GCNGC 2 cut(s) 25, 391
PluTI GGCGCC 2 cut(s) 80, 86
PpuMI RGGWCCY 1 cut(s) 95
Psp5II RGGWCCY 1 cut(s) 95
Psp6I CCWGG 4 cut(s) 26, 61, 142, 229
PspGI CCWGG 4 cut(s) 26, 61, 142, 229
PspN4I GGNNCC 4 cut(s) 78, 84, 96, 331
PspPI GGNCC 2 cut(s) 95, 188
PspPPI RGGWCCY 1 cut(s) 95
PstNI CAGNNNCTG 1 cut(s) 236
PvuII CAGCTG 1 cut(s) 23
RsaI GTAC 1 cut(s) 331
RsaNI GTAC 1 cut(s) 330
SapI GCTCTTC 1 cut(s) 249
SaqAI TTAA 2 cut(s) 42, 483
SatI GCNGC 2 cut(s) 24, 390
Sau3AI GATC 2 cut(s) 146, 321
Sau96I GGNCC 2 cut(s) 95, 188
ScrFI CCNGG 5 cut(s) 28, 63, 93, 144, 231
SduI GDGCHC 1 cut(s) 298
SfcI CTRYAG 1 cut(s) 237
SfoI GGCGCC 2 cut(s) 78, 84
SgrAI CRCCGGYG 2 cut(s) 73, 79
SinI GGWCC 2 cut(s) 95, 188
Sse9I AATT 2 cut(s) 438, 498
SsiI CCGC 1 cut(s) 48
SspDI GGCGCC 2 cut(s) 76, 82
SspMI CTAG 2 cut(s) 168, 404
StyD4I CCNGG 5 cut(s) 26, 61, 91, 142, 229
StyI CCWWGG 2 cut(s) 122, 167
TaaI ACNGT 2 cut(s) 238, 375
TaiI ACGT 2 cut(s) 223, 454
TasI AATT 2 cut(s) 438, 498
TfiI GAWTC 2 cut(s) 253, 422
Tru1I TTAA 2 cut(s) 42, 483
Tru9I TTAA 2 cut(s) 42, 483
TscAI CASTG 2 cut(s) 331, 380
TseFI GTSAC 2 cut(s) 87, 257
TseI GCWGC 2 cut(s) 23, 389
Tsp45I GTSAC 2 cut(s) 87, 257
TspDTI ATGAA 4 cut(s) 292, 371, 398, 431
TspRI CASTG 2 cut(s) 331, 380
VneI GTGCAC 1 cut(s) 294
VpaK11BI GGWCC 2 cut(s) 95, 188
XmaJI CCTAGG 1 cut(s) 167
XspI CTAG 2 cut(s) 168, 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.