RLG00000006531

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0009735
Physical Location & Seq
Reverse (-)
8183366 .. 8188921
5556 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006531

Sequence Viewer

Length: 444 bp
ATGGTTGGTGTTCGGGTATGTAGATCCAGCCTGACAAGCTTCAAGAGAGATAGTGTTCGCCAGTCGACCACAGCCACCGCTTCGATCTCTGCTGCTTCCATCCGCCCTCTGCTACTTCGATTCGCCCTCTGCTGCTTTGGGACTTTGATTCCCGGAGTTTCTAGCAATAGCCAGTTCATCTTTTTGTTCACTCTTCATAGTACAACACAGAGCAACCCTTCAAGTTATGGGTTCGTTCTTTCTTTTGCTTCCACAAATGTTTACATTGTCTATATGGGAGAAAGAAAGTATGGAAACCCCGAGCTGGCATCTGGGTCTCACCATGAGATTTTGTCATATGTATTGGCAAAATCAAGTTCAAGGCCTTTGATTTGGGAGGTCATCAGATTGCTCGCAGGGTCTGGAAGGCTTACTACGCAAAGTATCGTGCCAATGAGTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

148

Amino Acids

15.99

Weight (kDa)

9.87

Isoelectric Point (pI)

44.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 65
AciI CCGC 2 cut(s) 78, 103
AclWI GGATC 1 cut(s) 18
AfaI GTAC 1 cut(s) 202
AfiI CCNNNNNNNGG 1 cut(s) 304
AgsI TTSAA 3 cut(s) 43, 222, 360
AluBI AGCT 2 cut(s) 39, 304
AluI AGCT 2 cut(s) 39, 304
Alw26I GTCTC 1 cut(s) 321
AlwI GGATC 1 cut(s) 18
AlwNI CAGNNNCTG 1 cut(s) 401
Ama87I CYCGRG 1 cut(s) 299
AoxI GGCC 1 cut(s) 362
ApeKI GCWGC 2 cut(s) 92, 132
AsuC2I CCSGG 1 cut(s) 153
AsuHPI GGTGA 1 cut(s) 311
AvaI CYCGRG 1 cut(s) 299
BarI GAAGNNNNNNTAC 2 cut(s) 397, 429
BbvI GCAGC 2 cut(s) 79, 119
BccI CCATC 1 cut(s) 107
BcnI CCSGG 1 cut(s) 153
BcoDI GTCTC 1 cut(s) 321
BfaI CTAG 1 cut(s) 162
BisI GCNGC 2 cut(s) 93, 133
BlsI GCNGC 2 cut(s) 94, 134
Bme1390I CCNGG 1 cut(s) 153
BmeT110I CYCGRG 1 cut(s) 299
BmrFI CCNGG 1 cut(s) 153
BmsI GCATC 1 cut(s) 317
BpuMI CCSGG 1 cut(s) 153
BsaI GGTCTC 1 cut(s) 321
Bsc4I CCNNNNNNNGG 1 cut(s) 304
Bse1I ACTGG 2 cut(s) 61, 172
BseGI GGATG 1 cut(s) 99
BseLI CCNNNNNNNGG 1 cut(s) 304
BseNI ACTGG 2 cut(s) 61, 172
BseXI GCAGC 2 cut(s) 79, 119
BshFI GGCC 1 cut(s) 364
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 1 cut(s) 153
BslFI GGGAC 1 cut(s) 154
BslI CCNNNNNNNGG 1 cut(s) 304
BsmAI GTCTC 1 cut(s) 321
BsmFI GGGAC 1 cut(s) 154
BsnI GGCC 1 cut(s) 364
Bso31I GGTCTC 1 cut(s) 321
BsoBI CYCGRG 1 cut(s) 299
Bsp143I GATC 2 cut(s) 23, 84
BspACI CCGC 2 cut(s) 78, 103
BspANI GGCC 1 cut(s) 364
BspPI GGATC 1 cut(s) 18
BspTNI GGTCTC 1 cut(s) 321
BsrI ACTGG 2 cut(s) 61, 172
BssMI GATC 2 cut(s) 23, 84
Bst6I CTCTTC 1 cut(s) 198
BstC8I GCNNGC 2 cut(s) 306, 393
BstF5I GGATG 1 cut(s) 99
BstKTI GATC 2 cut(s) 26, 87
BstMAI GTCTC 1 cut(s) 321
BstMBI GATC 2 cut(s) 23, 84
BstMWI GCNNNNNNNGC 2 cut(s) 36, 415
BstSCI CCNGG 1 cut(s) 151
BstV1I GCAGC 2 cut(s) 79, 119
BstX2I RGATCY 1 cut(s) 23
BstYI RGATCY 1 cut(s) 23
BsuRI GGCC 1 cut(s) 364
BtsCI GGATG 1 cut(s) 99
Cac8I GCNNGC 2 cut(s) 306, 393
CaiI CAGNNNCTG 1 cut(s) 401
Csp6I GTAC 1 cut(s) 201
CviAII CATG 1 cut(s) 323
CviJI RGCY 7 cut(s) 30, 39, 74, 171, 304, 364, 409
CviKI_1 RGCY 7 cut(s) 30, 39, 74, 171, 304, 364, 409
CviQI GTAC 1 cut(s) 201
DpnI GATC 2 cut(s) 25, 86
DpnII GATC 2 cut(s) 23, 84
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
EciI GGCGGA 1 cut(s) 92
Eco147I AGGCCT 1 cut(s) 364
Eco31I GGTCTC 1 cut(s) 321
Eco88I CYCGRG 1 cut(s) 299
FaeI CATG 1 cut(s) 326
FaiI YATR 9 cut(s) 19, 198, 228, 273, 275, 291, 324, 337, 339
FaqI GGGAC 1 cut(s) 154
FatI CATG 1 cut(s) 322
FauNDI CATATG 1 cut(s) 337
FblI GTMKAC 1 cut(s) 65
Fnu4HI GCNGC 2 cut(s) 93, 133
FokI GGATG 1 cut(s) 86
Fsp4HI GCNGC 2 cut(s) 93, 133
FspBI CTAG 1 cut(s) 162
GluI GCNGC 2 cut(s) 93, 133
HaeIII GGCC 1 cut(s) 364
HapII CCGG 1 cut(s) 153
Hin1II CATG 1 cut(s) 326
HincII GTYRAC 1 cut(s) 66
HindII GTYRAC 1 cut(s) 66
HindIII AAGCTT 1 cut(s) 37
HinfI GANTC 2 cut(s) 120, 148
HpaII CCGG 1 cut(s) 153
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 3 cut(s) 66, 189, 262
Hpy188I TCNGA 1 cut(s) 386
Hpy188III TCNNGA 2 cut(s) 43, 402
Hpy8I GTNNAC 3 cut(s) 66, 189, 262
HpyAV CCTTC 2 cut(s) 228, 399
HpyF10VI GCNNNNNNNGC 2 cut(s) 36, 415
Hsp92II CATG 1 cut(s) 326
Kzo9I GATC 2 cut(s) 23, 84
LpnPI CCDG 9 cut(s) 40, 44, 74, 166, 185, 290, 297, 381, 387
Lsp1109I GCAGC 2 cut(s) 79, 119
LweI GCATC 1 cut(s) 317
MaeI CTAG 1 cut(s) 162
MalI GATC 2 cut(s) 25, 86
MboI GATC 2 cut(s) 23, 84
MboII GAAGA 1 cut(s) 185
MflI RGATCY 1 cut(s) 23
MnlI CCTC 3 cut(s) 117, 137, 370
MspI CCGG 1 cut(s) 153
MspR9I CCNGG 1 cut(s) 153
MwoI GCNNNNNNNGC 2 cut(s) 36, 415
NciI CCSGG 1 cut(s) 153
NdeI CATATG 1 cut(s) 337
NdeII GATC 2 cut(s) 23, 84
NlaIII CATG 1 cut(s) 326
PceI AGGCCT 1 cut(s) 364
PfeI GAWTC 2 cut(s) 120, 148
PfoI TCCNGGA 1 cut(s) 151
PkrI GCNGC 2 cut(s) 94, 134
PstNI CAGNNNCTG 1 cut(s) 401
PsuI RGATCY 1 cut(s) 23
RsaI GTAC 1 cut(s) 202
RsaNI GTAC 1 cut(s) 201
SalI GTCGAC 1 cut(s) 64
SatI GCNGC 2 cut(s) 93, 133
Sau3AI GATC 2 cut(s) 23, 84
ScrFI CCNGG 1 cut(s) 153
SetI ASST 3 cut(s) 41, 306, 381
SfaNI GCATC 1 cut(s) 317
SseBI AGGCCT 1 cut(s) 364
SsiI CCGC 2 cut(s) 78, 103
SspMI CTAG 1 cut(s) 162
StuI AGGCCT 1 cut(s) 364
StyD4I CCNGG 1 cut(s) 151
TaqI TCGA 3 cut(s) 65, 83, 118
TatI WGTACW 1 cut(s) 200
TfiI GAWTC 2 cut(s) 120, 148
TseI GCWGC 2 cut(s) 92, 132
TspDTI ATGAA 2 cut(s) 166, 185
XmiI GTMKAC 1 cut(s) 65
XspI CTAG 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.