RLG00000035857

ACT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0009735
Physical Location & Seq
Forward (+)
72706815 .. 72711706
4892 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035857

Sequence Viewer

Length: 681 bp
ATGATTGAAATCTGGAAATTTTGGACAGCTGTTAAAGTACTTAAGTTGACCGCCAATCGACCACAACCGCAGTGTTCACCAGTCGACCACCACCGCTTCGATCTCTGCTACTTCCATCCGCCCTCTGCTACTTCAATTCGCAGAGCCCTCTGCTGCTTTGGGACTTTGATTCCCGGAGTTTCGAGCAATATCCAGTTCATCTTTTTGGGATTATTGTCACCCTTTTGCTTCTTCTTGCTCTGCAGAGTTATGGGTTTTTTGCTTCCACAAATGTTTACATTGTCTATATGGGAGAAAGAAAGTATGGAAACCCCGAGCTGGCATCTGAGTCTCACCATGAGATTATGTCATATCTATTTGGAAGTTTATCTATTGAAGTTTCTTTGCTCTGACCGTAATGGATTATTGCATGATGTCACTAAAATTCTGTCTGATCTTGAGCTTTCGATTCAAAGGGTGAAAGTGACAACAACACCAGATGACAGGGTCTTGGACCTGTTCTTCATAACAGATAACATGGATCTTCTGCACACAAAGGAGAGGCAAGATAAGACATTGAAACAGTTACAGCAACATCAAGGTTCTTACCCTGCGAGAAACTCAATTGTTCTTACTGAGAGTAAACAAAAGGATGTACTTAAAGTTGCTGGTGCATTGTTTTCACATATTTGTGAGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

227

Amino Acids

26.15

Weight (kDa)

8.36

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 124 - 184 1.1e-09 ACT domain
ACT_ACR9_3rd PF24931 124 - 191 1e-05 ACR9-like, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 84
AciI CCGC 4 cut(s) 51, 68, 94, 119
AclWI GGATC 1 cut(s) 528
AcsI RAATTY 2 cut(s) 17, 423
AfaI GTAC 2 cut(s) 39, 636
AfiI CCNNNNNNNGG 1 cut(s) 318
AflII CTTAAG 1 cut(s) 41
AgsI TTSAA 5 cut(s) 8, 135, 376, 452, 559
AluBI AGCT 3 cut(s) 29, 318, 442
AluI AGCT 3 cut(s) 29, 318, 442
Alw26I GTCTC 1 cut(s) 335
AlwI GGATC 1 cut(s) 528
Ama87I CYCGRG 1 cut(s) 313
ApeKI GCWGC 1 cut(s) 153
ApoI RAATTY 2 cut(s) 17, 423
AspS9I GGNCC 1 cut(s) 493
AsuC2I CCSGG 1 cut(s) 174
AsuHPI GGTGA 4 cut(s) 69, 210, 325, 469
AvaI CYCGRG 1 cut(s) 313
AvaII GGWCC 1 cut(s) 493
BanII GRGCYC 1 cut(s) 148
BbvI GCAGC 1 cut(s) 140
BccI CCATC 1 cut(s) 123
BcnI CCSGG 1 cut(s) 174
BcoDI GTCTC 1 cut(s) 335
BfmI CTRYAG 1 cut(s) 241
BfrI CTTAAG 1 cut(s) 41
BisI GCNGC 1 cut(s) 154
BlsI GCNGC 1 cut(s) 155
BmcAI AGTACT 1 cut(s) 39
Bme1390I CCNGG 1 cut(s) 174
Bme18I GGWCC 1 cut(s) 493
BmeT110I CYCGRG 1 cut(s) 313
BmgT120I GGNCC 1 cut(s) 493
BmrFI CCNGG 1 cut(s) 174
BmsI GCATC 1 cut(s) 331
BpuEI CTTGAG 1 cut(s) 458
BpuMI CCSGG 1 cut(s) 174
BsaBI GATNNNNATC 1 cut(s) 8
Bsc4I CCNNNNNNNGG 1 cut(s) 318
Bse1I ACTGG 2 cut(s) 80, 193
Bse8I GATNNNNATC 1 cut(s) 8
BseGI GGATG 2 cut(s) 115, 637
BseJI GATNNNNATC 1 cut(s) 8
BseLI CCNNNNNNNGG 1 cut(s) 318
BseMII CTCAG 2 cut(s) 317, 606
BseNI ACTGG 2 cut(s) 80, 193
BseXI GCAGC 1 cut(s) 140
BsgI GTGCAG 1 cut(s) 512
BsiHKCI CYCGRG 1 cut(s) 313
BsiSI CCGG 1 cut(s) 174
BslFI GGGAC 1 cut(s) 175
BslI CCNNNNNNNGG 1 cut(s) 318
BsmAI GTCTC 1 cut(s) 335
BsmFI GGGAC 1 cut(s) 175
BsoBI CYCGRG 1 cut(s) 313
Bsp1286I GDGCHC 1 cut(s) 148
Bsp143I GATC 3 cut(s) 100, 433, 520
BspACI CCGC 4 cut(s) 51, 68, 94, 119
BspCNI CTCAG 2 cut(s) 318, 607
BspMAI CTGCAG 1 cut(s) 245
BspPI GGATC 1 cut(s) 528
BspTI CTTAAG 1 cut(s) 41
BsrI ACTGG 2 cut(s) 80, 193
BssMI GATC 3 cut(s) 100, 433, 520
Bst4CI ACNGT 2 cut(s) 395, 564
BstAFI CTTAAG 1 cut(s) 41
BstC8I GCNNGC 1 cut(s) 320
BstDEI CTNAG 2 cut(s) 326, 615
BstF5I GGATG 2 cut(s) 115, 637
BstKTI GATC 3 cut(s) 103, 436, 523
BstMAI GTCTC 1 cut(s) 335
BstMBI GATC 3 cut(s) 100, 433, 520
BstSCI CCNGG 1 cut(s) 172
BstSFI CTRYAG 1 cut(s) 241
BstV1I GCAGC 1 cut(s) 140
BstX2I RGATCY 1 cut(s) 520
BstYI RGATCY 1 cut(s) 520
BtsCI GGATG 2 cut(s) 115, 637
BtsI GCAGTG 1 cut(s) 77
BtsIMutI CAGTG 1 cut(s) 77
Cac8I GCNNGC 1 cut(s) 320
Cfr13I GGNCC 1 cut(s) 493
Csp6I GTAC 2 cut(s) 38, 635
CviAII CATG 3 cut(s) 337, 410, 517
CviJI RGCY 4 cut(s) 29, 146, 318, 442
CviKI_1 RGCY 4 cut(s) 29, 146, 318, 442
CviQI GTAC 2 cut(s) 38, 635
DdeI CTNAG 2 cut(s) 326, 615
DpnI GATC 3 cut(s) 102, 435, 522
DpnII GATC 3 cut(s) 100, 433, 520
EciI GGCGGA 1 cut(s) 108
Eco24I GRGCYC 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 493
Eco88I CYCGRG 1 cut(s) 313
EcoT38I GRGCYC 1 cut(s) 148
FaeI CATG 3 cut(s) 340, 413, 520
FaqI GGGAC 1 cut(s) 175
FatI CATG 3 cut(s) 336, 409, 516
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 1 cut(s) 154
FokI GGATG 2 cut(s) 102, 644
FriOI GRGCYC 1 cut(s) 148
Fsp4HI GCNGC 1 cut(s) 154
GluI GCNGC 1 cut(s) 154
HapII CCGG 1 cut(s) 174
Hin1II CATG 3 cut(s) 340, 413, 520
HincII GTYRAC 2 cut(s) 48, 85
HindII GTYRAC 2 cut(s) 48, 85
HinfI GANTC 3 cut(s) 169, 328, 448
HpaII CCGG 1 cut(s) 174
HphI GGTGA 4 cut(s) 69, 210, 325, 469
Hpy166II GTNNAC 5 cut(s) 48, 77, 85, 276, 623
Hpy188I TCNGA 3 cut(s) 327, 391, 433
Hpy188III TCNNGA 2 cut(s) 13, 437
Hpy8I GTNNAC 5 cut(s) 48, 77, 85, 276, 623
HpyCH4III ACNGT 2 cut(s) 395, 564
HpyCH4V TGCA 4 cut(s) 243, 409, 529, 653
HpyF3I CTNAG 2 cut(s) 326, 615
Hsp92II CATG 3 cut(s) 340, 413, 520
Kzo9I GATC 3 cut(s) 100, 433, 520
LpnPI CCDG 9 cut(s) 93, 187, 206, 304, 469, 489, 509, 603, 633
Lsp1109I GCAGC 1 cut(s) 140
LweI GCATC 1 cut(s) 331
MaeIII GTNAC 4 cut(s) 216, 415, 463, 564
MalI GATC 3 cut(s) 102, 435, 522
MboI GATC 3 cut(s) 100, 433, 520
MboII GAAGA 3 cut(s) 223, 493, 515
MfeI CAATTG 1 cut(s) 603
MflI RGATCY 1 cut(s) 520
MhlI GDGCHC 1 cut(s) 148
MluCI AATT 4 cut(s) 17, 135, 423, 603
MlyI GAGTC 1 cut(s) 337
MnlI CCTC 4 cut(s) 133, 158, 534, 667
MseI TTAA 3 cut(s) 33, 42, 639
MslI CAYNNNNRTG 1 cut(s) 669
MspA1I CMGCKG 1 cut(s) 29
MspCI CTTAAG 1 cut(s) 41
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 1 cut(s) 174
MunI CAATTG 1 cut(s) 603
NciI CCSGG 1 cut(s) 174
NdeII GATC 3 cut(s) 100, 433, 520
NlaIII CATG 3 cut(s) 340, 413, 520
NmuCI GTSAC 3 cut(s) 216, 415, 463
PfeI GAWTC 2 cut(s) 169, 448
PflFI GACNNNGTC 1 cut(s) 485
PfoI TCCNGGA 1 cut(s) 172
PkrI GCNGC 1 cut(s) 155
PleI GAGTC 1 cut(s) 336
PpsI GAGTC 1 cut(s) 336
PspPI GGNCC 1 cut(s) 493
PstI CTGCAG 1 cut(s) 245
PsuI RGATCY 1 cut(s) 520
PsyI GACNNNGTC 1 cut(s) 485
PvuII CAGCTG 1 cut(s) 29
RsaI GTAC 2 cut(s) 39, 636
RsaNI GTAC 2 cut(s) 38, 635
RseI CAYNNNNRTG 1 cut(s) 669
SalI GTCGAC 1 cut(s) 83
SaqAI TTAA 3 cut(s) 33, 42, 639
SatI GCNGC 1 cut(s) 154
Sau3AI GATC 3 cut(s) 100, 433, 520
Sau96I GGNCC 1 cut(s) 493
ScaI AGTACT 1 cut(s) 39
SchI GAGTC 1 cut(s) 337
ScrFI CCNGG 1 cut(s) 174
SduI GDGCHC 1 cut(s) 148
SetI ASST 6 cut(s) 31, 320, 444, 498, 583, 678
SfaNI GCATC 1 cut(s) 331
SfcI CTRYAG 1 cut(s) 241
SinI GGWCC 1 cut(s) 493
SmiMI CAYNNNNRTG 1 cut(s) 669
SmlI CTYRAG 2 cut(s) 41, 437
SmoI CTYRAG 2 cut(s) 41, 437
Sse9I AATT 4 cut(s) 17, 135, 423, 603
SsiI CCGC 4 cut(s) 51, 68, 94, 119
StyD4I CCNGG 1 cut(s) 172
TaaI ACNGT 2 cut(s) 395, 564
TaqI TCGA 5 cut(s) 58, 84, 99, 182, 446
TasI AATT 4 cut(s) 17, 135, 423, 603
TatI WGTACW 2 cut(s) 37, 634
TfiI GAWTC 2 cut(s) 169, 448
Tru1I TTAA 3 cut(s) 33, 42, 639
Tru9I TTAA 3 cut(s) 33, 42, 639
TscAI CASTG 1 cut(s) 77
TseFI GTSAC 3 cut(s) 216, 415, 463
TseI GCWGC 1 cut(s) 153
Tsp45I GTSAC 3 cut(s) 216, 415, 463
TspDTI ATGAA 2 cut(s) 187, 493
TspRI CASTG 1 cut(s) 77
Tth111I GACNNNGTC 1 cut(s) 485
Vha464I CTTAAG 1 cut(s) 41
VpaK11BI GGWCC 1 cut(s) 493
XapI RAATTY 2 cut(s) 17, 423
XmiI GTMKAC 1 cut(s) 84
ZrmI AGTACT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.