RLG00000014102

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009735
Physical Location & Seq
Reverse (-)
47182702 .. 47184828
2127 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014102

Sequence Viewer

Length: 687 bp
ATGGCTCCTCCTCCAGGGATTGTTGTCACCCTTTTGCTTCTTCTTGCCCTGCAGAGTTATGGGTTCGTTCTTTCTTTTGCTTCCACAAATGTTTACATTGTCTACATGGGAGAAAGAAAGTATGGAAACCCCGAGCTGGCATCTGAGTCTCACCATGAGGTTCTGTCATATGTATTTGGAAGCAAAGAAGCCACAAGGGAATCAATTATGTACAGCTACAAGCATGGATTTTCGGGGTTTGCTGCAGTTCTAACTCCATCTCAAGCCAAGCATATTGCAGATGCACCCGGTGTTGTTCGTGTCATTCCTCACAGAGTTAATCAGGCATTGGGTATATTATTGGAGTCATGGACATTGGAATTTGGCCCGAGTCTGAAAGCTTTAGAGATGAGGGTATGGGAGACATTCCATCTCGTTGGAGAGGCATATGTCAAGGAGGAGAAAAGTTCAAGCACTCTCATTGCAATAGGACATGGTACTCATACATCATTCTCTGCAGCCGGTGGTTTTATAGAAAATGCAAGTTTTATGGGACTTGCTTCAGGATTGGCAAGAGGAGGTGCTCCATCATCTTGGTTAGCTATTTACAAAGTTTGTTGGGCTACTGGTGGCTGCAGCACAGCAGACCTACTTGCTGCATTTGATGATGCAATTTTTGATGGTGTGGAGTGCTTTCGGTATCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

229

Amino Acids

24.58

Weight (kDa)

5.88

Isoelectric Point (pI)

31.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 31 - 105 2.4e-17 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 102
AcsI RAATTY 1 cut(s) 359
AcuI CTGAAG 1 cut(s) 525
AdeI CACNNNGTG 1 cut(s) 290
AfaI GTAC 2 cut(s) 212, 478
AfiI CCNNNNNNNGG 2 cut(s) 14, 136
AgsI TTSAA 1 cut(s) 450
AjnI CCWGG 1 cut(s) 13
AluBI AGCT 4 cut(s) 136, 216, 380, 581
AluI AGCT 4 cut(s) 136, 216, 380, 581
Alw21I GWGCWC 1 cut(s) 565
Alw26I GTCTC 2 cut(s) 153, 395
Ama87I CYCGRG 2 cut(s) 131, 367
AoxI GGCC 1 cut(s) 364
ApeKI GCWGC 5 cut(s) 242, 497, 612, 615, 635
ApoI RAATTY 1 cut(s) 359
AspS9I GGNCC 1 cut(s) 365
AsuC2I CCSGG 1 cut(s) 288
AsuHPI GGTGA 2 cut(s) 19, 143
AvaI CYCGRG 2 cut(s) 131, 367
Bbv12I GWGCWC 1 cut(s) 565
BbvI GCAGC 5 cut(s) 229, 509, 599, 622, 627
BccI CCATC 4 cut(s) 265, 417, 574, 653
BciT130I CCWGG 1 cut(s) 15
BcnI CCSGG 1 cut(s) 288
BcoDI GTCTC 2 cut(s) 153, 395
BfaI CTAG 1 cut(s) 685
BfmI CTRYAG 4 cut(s) 50, 243, 495, 613
BisI GCNGC 5 cut(s) 243, 498, 613, 616, 636
BlsI GCNGC 5 cut(s) 244, 499, 614, 617, 637
Bme1390I CCNGG 2 cut(s) 15, 288
BmeT110I CYCGRG 2 cut(s) 131, 367
BmgT120I GGNCC 1 cut(s) 365
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 2 cut(s) 15, 288
BmsI GCATC 3 cut(s) 149, 271, 637
BpuEI CTTGAG 1 cut(s) 246
BpuMI CCSGG 1 cut(s) 288
BsaJI CCNNGG 1 cut(s) 14
Bsc4I CCNNNNNNNGG 2 cut(s) 14, 136
Bse118I RCCGGY 1 cut(s) 500
Bse1I ACTGG 1 cut(s) 610
Bse3DI GCAATG 1 cut(s) 459
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 1 cut(s) 14
BseLI CCNNNNNNNGG 2 cut(s) 14, 136
BseMI GCAATG 1 cut(s) 459
BseMII CTCAG 1 cut(s) 135
BseNI ACTGG 1 cut(s) 610
BseRI GAGGAG 2 cut(s) 452, 570
BseXI GCAGC 5 cut(s) 229, 509, 599, 622, 627
BshFI GGCC 1 cut(s) 366
BsiHKAI GWGCWC 1 cut(s) 565
BsiHKCI CYCGRG 2 cut(s) 131, 367
BsiSI CCGG 2 cut(s) 288, 501
BslFI GGGAC 1 cut(s) 546
BslI CCNNNNNNNGG 2 cut(s) 14, 136
BsmAI GTCTC 2 cut(s) 153, 395
BsmFI GGGAC 1 cut(s) 546
BsnI GGCC 1 cut(s) 366
BsoBI CYCGRG 2 cut(s) 131, 367
Bsp1286I GDGCHC 1 cut(s) 565
Bsp1407I TGTACA 1 cut(s) 210
BspANI GGCC 1 cut(s) 366
BspCNI CTCAG 1 cut(s) 136
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 4 cut(s) 54, 247, 499, 617
BsrDI GCAATG 1 cut(s) 459
BsrFI RCCGGY 1 cut(s) 500
BsrGI TGTACA 1 cut(s) 210
BsrI ACTGG 1 cut(s) 610
BssAI RCCGGY 1 cut(s) 500
BssECI CCNNGG 1 cut(s) 14
Bst2UI CCWGG 1 cut(s) 15
BstAUI TGTACA 1 cut(s) 210
BstC8I GCNNGC 1 cut(s) 138
BstDEI CTNAG 1 cut(s) 144
BstENI CCTNNNNNAGG 1 cut(s) 12
BstMAI GTCTC 2 cut(s) 153, 395
BstNI CCWGG 1 cut(s) 15
BstSCI CCNGG 2 cut(s) 13, 286
BstSFI CTRYAG 4 cut(s) 50, 243, 495, 613
BstV1I GCAGC 5 cut(s) 229, 509, 599, 622, 627
BstXI CCANNNNNNTGG 2 cut(s) 416, 573
BsuRI GGCC 1 cut(s) 366
Cac8I GCNNGC 1 cut(s) 138
Cfr10I RCCGGY 1 cut(s) 500
Cfr13I GGNCC 1 cut(s) 365
Csp6I GTAC 2 cut(s) 211, 477
CviAII CATG 5 cut(s) 106, 155, 224, 348, 473
CviQI GTAC 2 cut(s) 211, 477
DdeI CTNAG 1 cut(s) 144
DraIII CACNNNGTG 1 cut(s) 290
Eco57I CTGAAG 1 cut(s) 525
Eco88I CYCGRG 2 cut(s) 131, 367
EcoNI CCTNNNNNAGG 1 cut(s) 12
EcoRII CCWGG 1 cut(s) 13
FaeI CATG 5 cut(s) 109, 158, 227, 351, 476
FaqI GGGAC 1 cut(s) 546
FatI CATG 5 cut(s) 105, 154, 223, 347, 472
FauNDI CATATG 2 cut(s) 169, 427
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 5 cut(s) 243, 498, 613, 616, 636
Fsp4HI GCNGC 5 cut(s) 243, 498, 613, 616, 636
FspBI CTAG 1 cut(s) 685
GluI GCNGC 5 cut(s) 243, 498, 613, 616, 636
HaeIII GGCC 1 cut(s) 366
HapII CCGG 2 cut(s) 288, 501
Hin1II CATG 5 cut(s) 109, 158, 227, 351, 476
HindIII AAGCTT 1 cut(s) 378
HinfI GANTC 4 cut(s) 146, 200, 344, 370
HpaII CCGG 2 cut(s) 288, 501
HphI GGTGA 2 cut(s) 19, 143
Hpy166II GTNNAC 2 cut(s) 94, 103
Hpy188I TCNGA 2 cut(s) 145, 375
Hpy188III TCNNGA 1 cut(s) 543
Hpy8I GTNNAC 2 cut(s) 94, 103
HpyF3I CTNAG 1 cut(s) 144
Hsp92II CATG 5 cut(s) 109, 158, 227, 351, 476
LmnI GCTCC 2 cut(s) 10, 568
LpnPI CCDG 8 cut(s) 27, 62, 122, 301, 308, 514, 528, 591
Lsp1109I GCAGC 5 cut(s) 229, 509, 599, 622, 627
LweI GCATC 3 cut(s) 149, 271, 637
MaeI CTAG 1 cut(s) 685
MaeIII GTNAC 1 cut(s) 25
MboII GAAGA 1 cut(s) 32
MhlI GDGCHC 1 cut(s) 565
MluCI AATT 3 cut(s) 204, 359, 651
MlyI GAGTC 3 cut(s) 155, 353, 379
MmeI TCCRAC 1 cut(s) 397
MnlI CCTC 9 cut(s) 18, 21, 151, 318, 384, 415, 430, 548, 551
MseI TTAA 1 cut(s) 318
MspI CCGG 2 cut(s) 288, 501
MspR9I CCNGG 2 cut(s) 15, 288
MvaI CCWGG 1 cut(s) 15
NciI CCSGG 1 cut(s) 288
NdeI CATATG 2 cut(s) 169, 427
NlaIII CATG 5 cut(s) 109, 158, 227, 351, 476
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 25
PfeI GAWTC 1 cut(s) 200
PkrI GCNGC 5 cut(s) 244, 499, 614, 617, 637
PleI GAGTC 3 cut(s) 154, 352, 378
PpsI GAGTC 3 cut(s) 154, 352, 378
Psp6I CCWGG 1 cut(s) 13
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 365
PstI CTGCAG 4 cut(s) 54, 247, 499, 617
RsaI GTAC 2 cut(s) 212, 478
RsaNI GTAC 2 cut(s) 211, 477
SaqAI TTAA 1 cut(s) 318
SatI GCNGC 5 cut(s) 243, 498, 613, 616, 636
Sau96I GGNCC 1 cut(s) 365
SchI GAGTC 3 cut(s) 155, 353, 379
ScrFI CCNGG 2 cut(s) 15, 288
SduI GDGCHC 1 cut(s) 565
SetI ASST 7 cut(s) 138, 162, 218, 382, 562, 583, 630
SfaNI GCATC 3 cut(s) 149, 271, 637
SfcI CTRYAG 4 cut(s) 50, 243, 495, 613
SmlI CTYRAG 1 cut(s) 261
SmoI CTYRAG 1 cut(s) 261
Sse9I AATT 3 cut(s) 204, 359, 651
SspMI CTAG 1 cut(s) 685
StyD4I CCNGG 2 cut(s) 13, 286
TasI AATT 3 cut(s) 204, 359, 651
TatI WGTACW 1 cut(s) 210
TfiI GAWTC 1 cut(s) 200
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TseFI GTSAC 1 cut(s) 25
TseI GCWGC 5 cut(s) 242, 497, 612, 615, 635
Tsp45I GTSAC 1 cut(s) 25
XagI CCTNNNNNAGG 1 cut(s) 12
XapI RAATTY 1 cut(s) 359
XmiI GTMKAC 1 cut(s) 102
XspI CTAG 1 cut(s) 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.