Rw4G018010

ACT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009735
Physical Location & Seq
Forward (+)
40195572 .. 40199778
4207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G018010.1

Sequence Viewer

Length: 684 bp
ATGAACCCAACTACATGTTCCTCGCCCCCTGCAGTTTATCTATTGAAGTTTCTTTGCTCTGACCGTAATGGATTATTGCATGATGTCACTGAAATTCTGTCTGATCTTGAGCTTTCGATTCAAAGGGTGAAAGTGACAACAACACCATATGACAGGGTCTTGGACCTGTTCTTCATAACAGATAACATGGATCTTCTGCACACAAAGGAGCGGCAAGATAAGACATTGAAACAGTTGCAAGCCGTATTGGGTGAGTCGTGTACCAGTTGCGAACTTCAGTTGGTAGGTCCTGAGTATGATCCCCACCATGGCATTCCTTCTCTGTCTCCATTAGTAGCTGAAGAGCTATTTAGGTGTGAGCTGTTGGATAAAGAAATCCGTTCTCAAGCTCTTAGTCCTGATATGACGAAATTGAAGAACATCCACTGTGCTGATCACAAGGGTCTCTTGTATGACATTATGAGAACTTTGAAAGACAGCAACATCAAGACAATGGGATTTGCAAGTACATATTGTCTGATCAAGCCTTCAGACAACAGCAAGCATATAAGCCCCTGTTGTCTGGCACATCCTTCAGACAATAGTAAGTATATAAGCTGCTGTTGTTCTTCGTGGTATTCAGACAACAGACATGATAGTAGCTGCTGTTGTACTAAACTTTATCAGACAACAGCAAGTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

227

Amino Acids

25.55

Weight (kDa)

5.96

Isoelectric Point (pI)

51.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 14 - 74 1e-08 ACT domain
ACT_ACR9_3rd PF24931 130 - 164 9.7e-10 ACR9-like, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 211
AciI CCGC 1 cut(s) 211
AclWI GGATC 2 cut(s) 198, 293
AcsI RAATTY 1 cut(s) 93
AcuI CTGAAG 4 cut(s) 260, 360, 513, 558
AfaI GTAC 3 cut(s) 262, 508, 652
AfiI CCNNNNNNNGG 1 cut(s) 308
AflIII ACRYGT 1 cut(s) 14
AgsI TTSAA 5 cut(s) 46, 122, 229, 415, 472
AjuI GAANNNNNNNTTGG 1 cut(s) 33
AluBI AGCT 7 cut(s) 112, 338, 346, 361, 389, 597, 642
AluI AGCT 7 cut(s) 112, 338, 346, 361, 389, 597, 642
Alw26I GTCTC 2 cut(s) 330, 449
AlwI GGATC 2 cut(s) 198, 293
ApeKI GCWGC 2 cut(s) 597, 642
ApoI RAATTY 1 cut(s) 93
AspS9I GGNCC 2 cut(s) 163, 287
AsuHPI GGTGA 2 cut(s) 139, 263
AvaII GGWCC 2 cut(s) 163, 287
BbvI GCAGC 2 cut(s) 584, 629
BceAI ACGGC 1 cut(s) 227
BclI TGATCA 2 cut(s) 433, 519
BcoDI GTCTC 2 cut(s) 330, 449
BfmI CTRYAG 1 cut(s) 30
BisI GCNGC 3 cut(s) 212, 598, 643
BlsI GCNGC 3 cut(s) 213, 599, 644
Bme18I GGWCC 2 cut(s) 163, 287
BmgT120I GGNCC 2 cut(s) 163, 287
BpuEI CTTGAG 2 cut(s) 128, 369
BsaI GGTCTC 1 cut(s) 449
BsaJI CCNNGG 1 cut(s) 307
Bsc4I CCNNNNNNNGG 1 cut(s) 308
Bse1I ACTGG 1 cut(s) 264
BseDI CCNNGG 1 cut(s) 307
BseGI GGATG 2 cut(s) 420, 568
BseLI CCNNNNNNNGG 1 cut(s) 308
BseMII CTCAG 1 cut(s) 282
BseNI ACTGG 1 cut(s) 264
BseXI GCAGC 2 cut(s) 584, 629
BsgI GTGCAG 1 cut(s) 182
BslI CCNNNNNNNGG 1 cut(s) 308
BsmAI GTCTC 2 cut(s) 330, 449
BsmI GAATGC 1 cut(s) 312
Bso31I GGTCTC 1 cut(s) 449
Bsp143I GATC 5 cut(s) 103, 190, 298, 433, 519
Bsp19I CCATGG 1 cut(s) 307
BspACI CCGC 1 cut(s) 211
BspCNI CTCAG 1 cut(s) 283
BspMAI CTGCAG 1 cut(s) 34
BspPI GGATC 2 cut(s) 198, 293
BspQI GCTCTTC 1 cut(s) 336
BspTNI GGTCTC 1 cut(s) 449
BsrBI CCGCTC 1 cut(s) 211
BsrI ACTGG 1 cut(s) 264
BssECI CCNNGG 1 cut(s) 307
BssMI GATC 5 cut(s) 103, 190, 298, 433, 519
BssT1I CCWWGG 1 cut(s) 307
Bst4CI ACNGT 3 cut(s) 65, 234, 428
Bst6I CTCTTC 1 cut(s) 336
BstC8I GCNNGC 2 cut(s) 240, 542
BstDEI CTNAG 2 cut(s) 291, 392
BstDSI CCRYGG 1 cut(s) 307
BstF5I GGATG 2 cut(s) 420, 568
BstKTI GATC 5 cut(s) 106, 193, 301, 436, 522
BstMAI GTCTC 2 cut(s) 330, 449
BstMBI GATC 5 cut(s) 103, 190, 298, 433, 519
BstNSI RCATGY 1 cut(s) 18
BstSFI CTRYAG 1 cut(s) 30
BstV1I GCAGC 2 cut(s) 584, 629
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
BtgI CCRYGG 1 cut(s) 307
BtsCI GGATG 2 cut(s) 420, 568
BtsIMutI CAGTG 2 cut(s) 87, 424
Cac8I GCNNGC 2 cut(s) 240, 542
Cfr13I GGNCC 2 cut(s) 163, 287
Csp6I GTAC 3 cut(s) 261, 507, 651
CviAII CATG 5 cut(s) 15, 80, 187, 308, 632
CviQI GTAC 3 cut(s) 261, 507, 651
DdeI CTNAG 2 cut(s) 291, 392
DpnI GATC 5 cut(s) 105, 192, 300, 435, 521
DpnII GATC 5 cut(s) 103, 190, 298, 433, 519
Eam1104I CTCTTC 1 cut(s) 336
EarI CTCTTC 1 cut(s) 336
Eco130I CCWWGG 1 cut(s) 307
Eco31I GGTCTC 1 cut(s) 449
Eco47I GGWCC 2 cut(s) 163, 287
Eco57I CTGAAG 4 cut(s) 260, 360, 513, 558
EcoO109I RGGNCCY 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 307
ErhI CCWWGG 1 cut(s) 307
FaeI CATG 5 cut(s) 18, 83, 190, 311, 635
FalI AAGNNNNNCTT 2 cut(s) 431, 463
FatI CATG 5 cut(s) 14, 79, 186, 307, 631
FauNDI CATATG 1 cut(s) 148
FbaI TGATCA 2 cut(s) 433, 519
Fnu4HI GCNGC 3 cut(s) 212, 598, 643
FokI GGATG 2 cut(s) 407, 555
Fsp4HI GCNGC 3 cut(s) 212, 598, 643
GluI GCNGC 3 cut(s) 212, 598, 643
Hin1II CATG 5 cut(s) 18, 83, 190, 311, 635
HinfI GANTC 2 cut(s) 118, 254
HphI GGTGA 2 cut(s) 139, 263
Hpy166II GTNNAC 1 cut(s) 261
Hpy188I TCNGA 7 cut(s) 61, 103, 519, 532, 577, 622, 666
Hpy188III TCNNGA 4 cut(s) 107, 290, 398, 487
Hpy8I GTNNAC 1 cut(s) 261
HpyAV CCTTC 3 cut(s) 327, 537, 582
HpyCH4III ACNGT 3 cut(s) 65, 234, 428
HpyCH4V TGCA 5 cut(s) 32, 79, 199, 238, 503
HpyF3I CTNAG 2 cut(s) 291, 392
Hsp92II CATG 5 cut(s) 18, 83, 190, 311, 635
Ksp22I TGATCA 2 cut(s) 433, 519
Kzo9I GATC 5 cut(s) 103, 190, 298, 433, 519
LguI GCTCTTC 1 cut(s) 336
LmnI GCTCC 1 cut(s) 208
LpnPI CCDG 8 cut(s) 42, 139, 179, 277, 303, 411, 548, 568
Lsp1109I GCAGC 2 cut(s) 584, 629
MaeIII GTNAC 2 cut(s) 85, 133
MalI GATC 5 cut(s) 105, 192, 300, 435, 521
MbiI CCGCTC 1 cut(s) 211
MboI GATC 5 cut(s) 103, 190, 298, 433, 519
MboII GAAGA 5 cut(s) 163, 185, 353, 427, 600
MflI RGATCY 1 cut(s) 190
MluCI AATT 2 cut(s) 93, 410
MlyI GAGTC 1 cut(s) 263
MmeI TCCRAC 1 cut(s) 345
MnlI CCTC 1 cut(s) 31
Mva1269I GAATGC 1 cut(s) 312
NcoI CCATGG 1 cut(s) 307
NdeI CATATG 1 cut(s) 148
NdeII GATC 5 cut(s) 103, 190, 298, 433, 519
NlaIII CATG 5 cut(s) 18, 83, 190, 311, 635
NmuCI GTSAC 2 cut(s) 85, 133
NspI RCATGY 1 cut(s) 18
PciI ACATGT 1 cut(s) 14
PciSI GCTCTTC 1 cut(s) 336
PctI GAATGC 1 cut(s) 312
PfeI GAWTC 1 cut(s) 118
PflFI GACNNNGTC 1 cut(s) 155
PkrI GCNGC 3 cut(s) 213, 599, 644
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
PpuMI RGGWCCY 1 cut(s) 287
PscI ACATGT 1 cut(s) 14
Psp5II RGGWCCY 1 cut(s) 287
PspPI GGNCC 2 cut(s) 163, 287
PspPPI RGGWCCY 1 cut(s) 287
PstI CTGCAG 1 cut(s) 34
PsuI RGATCY 1 cut(s) 190
PsyI GACNNNGTC 1 cut(s) 155
RsaI GTAC 3 cut(s) 262, 508, 652
RsaNI GTAC 3 cut(s) 261, 507, 651
SapI GCTCTTC 1 cut(s) 336
SatI GCNGC 3 cut(s) 212, 598, 643
Sau3AI GATC 5 cut(s) 103, 190, 298, 433, 519
Sau96I GGNCC 2 cut(s) 163, 287
SchI GAGTC 1 cut(s) 263
SfcI CTRYAG 1 cut(s) 30
SinI GGWCC 2 cut(s) 163, 287
SmlI CTYRAG 2 cut(s) 107, 384
SmoI CTYRAG 2 cut(s) 107, 384
Sse9I AATT 2 cut(s) 93, 410
SsiI CCGC 1 cut(s) 211
StyI CCWWGG 1 cut(s) 307
TaaI ACNGT 3 cut(s) 65, 234, 428
TaqI TCGA 1 cut(s) 116
TasI AATT 2 cut(s) 93, 410
TatI WGTACW 2 cut(s) 506, 650
TauI GCSGC 1 cut(s) 214
TfiI GAWTC 1 cut(s) 118
TscAI CASTG 2 cut(s) 94, 431
TseFI GTSAC 2 cut(s) 85, 133
TseI GCWGC 2 cut(s) 597, 642
Tsp45I GTSAC 2 cut(s) 85, 133
TspDTI ATGAA 2 cut(s) 17, 163
TspGWI ACGGA 1 cut(s) 368
TspRI CASTG 2 cut(s) 94, 431
Tth111I GACNNNGTC 1 cut(s) 155
VpaK11BI GGWCC 2 cut(s) 163, 287
XapI RAATTY 1 cut(s) 93
XceI RCATGY 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.