RLG00000012244

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009735
Physical Location & Seq
Reverse (-)
16570361 .. 16576358
5998 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012244

Sequence Viewer

Length: 414 bp
ATGGCTCCTCCTCCAGGGATTGTTGTCCCCCTTTTGCTTCTTCTTGCTCTGCAGAGTTATGGGTTCGTTCTTTCTTTTGCTTCCACAAATGTTTACATTGTCTATATGGGAGAAAGAAAGTATGGAAACCCCGAGCTGGCATCTGAGACTCACCATGAGATTCTGTCATATGTATTTGGAAGCAAAGAAGCCACAAGGGAATCAATTCTGTACAGCTACAAGCATGGATTTTCGGGGTTTGCTGCAGTTCTAACTCCATCTCAAGCCAAGCATATTGCAGGTGTTACAATGTTGGTTCTTACTGAGGGTAAACAAAAGGATGTACTTAAAGCTGCTGGTGCATTGTTTTCACATATTTGTGAGGTGAAGTATGCTGCTCAGTTTCAGGAAAATCAGAAGAGTGATTCCGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

138

Amino Acids

14.97

Weight (kDa)

6.5

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 31 - 93 1e-13 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 269
Acc36I ACCTGC 1 cut(s) 269
AfaI GTAC 2 cut(s) 212, 324
AfiI CCNNNNNNNGG 2 cut(s) 14, 136
AjnI CCWGG 1 cut(s) 13
AluBI AGCT 3 cut(s) 136, 216, 332
AluI AGCT 3 cut(s) 136, 216, 332
Alw26I GTCTC 1 cut(s) 140
Ama87I CYCGRG 1 cut(s) 131
ApeKI GCWGC 3 cut(s) 242, 332, 374
Asp700I GAANNNNTTC 1 cut(s) 204
AsuHPI GGTGA 2 cut(s) 143, 376
AvaI CYCGRG 1 cut(s) 131
BbvI GCAGC 3 cut(s) 229, 319, 361
BccI CCATC 1 cut(s) 265
BciT130I CCWGG 1 cut(s) 15
BcoDI GTCTC 1 cut(s) 140
BfaI CTAG 1 cut(s) 412
BfmI CTRYAG 2 cut(s) 50, 243
BfuAI ACCTGC 1 cut(s) 269
BisI GCNGC 3 cut(s) 243, 333, 375
BlsI GCNGC 3 cut(s) 244, 334, 376
Bme1390I CCNGG 1 cut(s) 15
BmeT110I CYCGRG 1 cut(s) 131
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 15
BmsI GCATC 1 cut(s) 149
BpuEI CTTGAG 1 cut(s) 246
BsaJI CCNNGG 1 cut(s) 14
Bsc4I CCNNNNNNNGG 2 cut(s) 14, 136
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 1 cut(s) 14
BseGI GGATG 1 cut(s) 325
BseLI CCNNNNNNNGG 2 cut(s) 14, 136
BseMII CTCAG 3 cut(s) 135, 294, 392
BseXI GCAGC 3 cut(s) 229, 319, 361
BsiHKCI CYCGRG 1 cut(s) 131
BslFI GGGAC 1 cut(s) 11
BslI CCNNNNNNNGG 2 cut(s) 14, 136
BsmAI GTCTC 1 cut(s) 140
BsmFI GGGAC 1 cut(s) 11
BsoBI CYCGRG 1 cut(s) 131
Bsp1407I TGTACA 1 cut(s) 210
BspCNI CTCAG 3 cut(s) 136, 295, 391
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 2 cut(s) 54, 247
BspMI ACCTGC 1 cut(s) 269
BsrGI TGTACA 1 cut(s) 210
BssECI CCNNGG 1 cut(s) 14
Bst2UI CCWGG 1 cut(s) 15
Bst6I CTCTTC 1 cut(s) 392
BstAUI TGTACA 1 cut(s) 210
BstC8I GCNNGC 1 cut(s) 138
BstDEI CTNAG 3 cut(s) 144, 303, 378
BstENI CCTNNNNNAGG 1 cut(s) 12
BstF5I GGATG 1 cut(s) 325
BstMAI GTCTC 1 cut(s) 140
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstNI CCWGG 1 cut(s) 15
BstSCI CCNGG 1 cut(s) 13
BstSFI CTRYAG 2 cut(s) 50, 243
BstV1I GCAGC 3 cut(s) 229, 319, 361
BtsCI GGATG 1 cut(s) 325
BveI ACCTGC 1 cut(s) 269
Cac8I GCNNGC 1 cut(s) 138
Csp6I GTAC 2 cut(s) 211, 323
CviAII CATG 2 cut(s) 155, 224
CviJI RGCY 6 cut(s) 5, 136, 191, 216, 266, 332
CviKI_1 RGCY 6 cut(s) 5, 136, 191, 216, 266, 332
CviQI GTAC 2 cut(s) 211, 323
DdeI CTNAG 3 cut(s) 144, 303, 378
Eam1104I CTCTTC 1 cut(s) 392
EarI CTCTTC 1 cut(s) 392
Eco88I CYCGRG 1 cut(s) 131
EcoNI CCTNNNNNAGG 1 cut(s) 12
EcoRII CCWGG 1 cut(s) 13
FaeI CATG 2 cut(s) 158, 227
FaqI GGGAC 1 cut(s) 11
FatI CATG 2 cut(s) 154, 223
FauNDI CATATG 1 cut(s) 169
Fnu4HI GCNGC 3 cut(s) 243, 333, 375
FokI GGATG 1 cut(s) 332
Fsp4HI GCNGC 3 cut(s) 243, 333, 375
FspBI CTAG 1 cut(s) 412
GluI GCNGC 3 cut(s) 243, 333, 375
Hin1II CATG 2 cut(s) 158, 227
HinfI GANTC 4 cut(s) 148, 160, 200, 404
HphI GGTGA 2 cut(s) 143, 376
Hpy166II GTNNAC 2 cut(s) 94, 311
Hpy188I TCNGA 3 cut(s) 145, 396, 409
Hpy188III TCNNGA 1 cut(s) 386
Hpy8I GTNNAC 2 cut(s) 94, 311
HpyCH4V TGCA 4 cut(s) 52, 245, 278, 341
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpyF3I CTNAG 3 cut(s) 144, 303, 378
Hsp92II CATG 2 cut(s) 158, 227
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 5 cut(s) 27, 122, 264, 321, 371
Lsp1109I GCAGC 3 cut(s) 229, 319, 361
LweI GCATC 1 cut(s) 149
MaeI CTAG 1 cut(s) 412
MaeIII GTNAC 1 cut(s) 283
MboII GAAGA 2 cut(s) 32, 409
MluCI AATT 1 cut(s) 204
MlyI GAGTC 1 cut(s) 142
MnlI CCTC 4 cut(s) 18, 21, 298, 355
MroXI GAANNNNTTC 1 cut(s) 204
MseI TTAA 1 cut(s) 327
MslI CAYNNNNRTG 1 cut(s) 357
MspR9I CCNGG 1 cut(s) 15
MvaI CCWGG 1 cut(s) 15
MwoI GCNNNNNNNGC 1 cut(s) 338
NdeI CATATG 1 cut(s) 169
NlaIII CATG 2 cut(s) 158, 227
NlaIV GGNNCC 1 cut(s) 6
PaqCI CACCTGC 1 cut(s) 269
PdmI GAANNNNTTC 1 cut(s) 204
PfeI GAWTC 3 cut(s) 160, 200, 404
PkrI GCNGC 3 cut(s) 244, 334, 376
PleI GAGTC 1 cut(s) 142
PpsI GAGTC 1 cut(s) 142
Psp6I CCWGG 1 cut(s) 13
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 1 cut(s) 6
PstI CTGCAG 2 cut(s) 54, 247
RsaI GTAC 2 cut(s) 212, 324
RsaNI GTAC 2 cut(s) 211, 323
RseI CAYNNNNRTG 1 cut(s) 357
SaqAI TTAA 1 cut(s) 327
SatI GCNGC 3 cut(s) 243, 333, 375
SchI GAGTC 1 cut(s) 142
ScrFI CCNGG 1 cut(s) 15
SetI ASST 5 cut(s) 138, 218, 283, 334, 366
SfaNI GCATC 1 cut(s) 149
SfcI CTRYAG 2 cut(s) 50, 243
SmiMI CAYNNNNRTG 1 cut(s) 357
SmlI CTYRAG 1 cut(s) 261
SmoI CTYRAG 1 cut(s) 261
Sse9I AATT 1 cut(s) 204
SspMI CTAG 1 cut(s) 412
StyD4I CCNGG 1 cut(s) 13
TasI AATT 1 cut(s) 204
TatI WGTACW 2 cut(s) 210, 322
TfiI GAWTC 3 cut(s) 160, 200, 404
Tru1I TTAA 1 cut(s) 327
Tru9I TTAA 1 cut(s) 327
TseI GCWGC 3 cut(s) 242, 332, 374
XagI CCTNNNNNAGG 1 cut(s) 12
XmnI GAANNNNTTC 1 cut(s) 204
XspI CTAG 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.