Rorug03G0199800

ACT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0009735
Physical Location & Seq
Reverse (-)
17614517 .. 17616137
1621 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0199800.1

Sequence Viewer

Length: 1416 bp
ATGGCTGATGTTACTCGAGTGAGCAGCTTGTCCAAAAGATGTAGAGGGCTTCATCTATCAAATCCCACATTGAATTTCGATTTATTTCCCTCTGAGTCCACCCTCACATTTGATAAGAGGTTGGTGTTGTTGAACTCCTTGGATCGCTTCCTGATTCAACGAGGGAATAACAAGGTAAAATACTTTTCCATTATTTGGACTTCTGAATACCATTTTGGAGAAACCGAAATAGAAAGTCACTGTGGTCTTGAGAAATCACGAATTCTGGGTTGGATCCAATATGCAGTACGTTGTAATGTTGAAGAGCTTTATGTTTCCTTTGGAATTGCGATGCCAACTCTAGGAGATTTTCTTCAGGATGATGATATAGATTTTCCTTCTTGTGTCTTTCTTTGCGAGTCTTTGAGGTACTTAACCCTGGACATGAAGTGTGCAGTTCTTGAACCTCCCTCCTTTTCTACTTCTCCTGCCTCTAATCTTGTGTCCTTGATTTTGAGAAATGTAACTATAGAAGATGATGGAGGGTTTTGCAAATGGGTCTCCTACTGTTGTAGGTGTATTAGGAAATTGAGGCTGGAACGGGTTTATGAGTTGGAAAACATCACCATTGAAAGTTCGACTTTGGAGTCATTCTCGGTCCAGTGTCCCTGTTATTTCGACATTGACCGTCTTGTAATAGCTGGTGATAAAATTGAAAATATAGATATAGATTGGGAGTATGATTCAGACAAGCACAACAACAAAAGTTTGTTGAGTATTTCGGCCCCAAATCTGAAGTATCTGAAATGGGTTGGGAATATGTTGAACCATCAGCATCTTGGGGAAATGAATTGTTTGGAACAAGTTGAGATTTCTCTGACTTCTTCTGATGTAAATGATTTGGATAGCGTTTGTGAGCAGTTCTTGTGCAGTATAAGCAGTGTTAAAGTTCTTCTTCTAGACCAAGTGACTGCCAAGACTTTGTTCAGGGAAGGTTTCACACCTGCCCCATTGGGCAATGTTTGGTACTTGCTTATGTCTATTTGGTGCAGTATTGATGACAACCTTGTGCTAGCCGTGACCTCTCTTTTCAGGGCTGTGCCTAATTTGACTACTTTGAACATACCATCATCTCCTGAGTTTCCTTTTCATGGTGGTGGTGATAATGTATCTGGATTTGATAGGGGATATTGGGAACTCCAGGGGCTTGATTTTGTTTCTAAGCTAGAGGAGGTAACCATAGAGCTTACCACTGGGTCCAATGGATTTGAATTAGCGAGGTACATGCTTGAGCATGCTGGTAATTTGAAGAAAATGTTTGTTATTACTACACCCGATCAATCAAGTTTAGTACCGGAAATACGGAGAACCAACCGTACTTCCAATGCTAGAGTTGTCTTTCTGGTAAAACGGAAGCTGCCTCCTCGTCGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

471

Amino Acids

53.53

Weight (kDa)

5.17

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 90 - 287 5.4e-08 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 991
AasI GACNNNNNNGTC 1 cut(s) 625
Acc36I ACCTGC 1 cut(s) 991
AccB7I CCANNNNNTGG 1 cut(s) 195
AclWI GGATC 3 cut(s) 150, 268, 281
AcsI RAATTY 2 cut(s) 73, 261
AcuI CTGAAG 2 cut(s) 338, 794
AfaI GTAC 6 cut(s) 288, 410, 1007, 1262, 1332, 1357
AfiI CCNNNNNNNGG 3 cut(s) 195, 341, 1130
AjnI CCWGG 2 cut(s) 417, 1179
AjuI GAANNNNNNNTTGG 6 cut(s) 198, 230, 253, 285, 947, 979
AluBI AGCT 6 cut(s) 27, 307, 680, 1204, 1225, 1396
AluI AGCT 6 cut(s) 27, 307, 680, 1204, 1225, 1396
Alw26I GTCTC 1 cut(s) 544
AlwI GGATC 3 cut(s) 150, 268, 281
Ama87I CYCGRG 1 cut(s) 15
AoxI GGCC 1 cut(s) 762
ApeKI GCWGC 2 cut(s) 24, 1396
ApoI RAATTY 2 cut(s) 73, 261
AspS9I GGNCC 3 cut(s) 637, 763, 1236
AsuHPI GGTGA 3 cut(s) 595, 695, 1151
AsuNHI GCTAGC 1 cut(s) 1051
AvaI CYCGRG 1 cut(s) 15
AvaII GGWCC 2 cut(s) 637, 1236
BamHI GGATCC 1 cut(s) 273
BbvI GCAGC 2 cut(s) 36, 1383
BccI CCATC 3 cut(s) 512, 816, 1114
BceAI ACGGC 1 cut(s) 1040
BcgI CGANNNNNNTGC 2 cut(s) 1246, 1280
BciT130I CCWGG 2 cut(s) 419, 1181
BcoDI GTCTC 1 cut(s) 544
BfaI CTAG 5 cut(s) 341, 938, 1052, 1205, 1368
BfmI CTRYAG 1 cut(s) 507
BfuAI ACCTGC 1 cut(s) 991
BisI GCNGC 2 cut(s) 25, 1397
BlsI GCNGC 2 cut(s) 26, 1398
Bme1390I CCNGG 2 cut(s) 419, 1181
Bme18I GGWCC 2 cut(s) 637, 1236
BmeT110I CYCGRG 1 cut(s) 15
BmgT120I GGNCC 3 cut(s) 637, 763, 1236
BmiI GGNNCC 3 cut(s) 275, 765, 1237
BmrFI CCNGG 2 cut(s) 419, 1181
BmrI ACTGGG 1 cut(s) 1242
BmsI GCATC 2 cut(s) 321, 823
BmtI GCTAGC 1 cut(s) 1055
BmuI ACTGGG 1 cut(s) 1242
BplI GAGNNNNNCTC 2 cut(s) 617, 649
BpmI CTGGAG 1 cut(s) 1163
BpuEI CTTGAG 2 cut(s) 269, 1289
BsaI GGTCTC 1 cut(s) 544
BsaJI CCNNGG 3 cut(s) 138, 417, 1180
BsaWI WCCGGW 1 cut(s) 1333
Bsc4I CCNNNNNNNGG 3 cut(s) 195, 341, 1130
Bse1I ACTGG 2 cut(s) 640, 1237
Bse3DI GCAATG 1 cut(s) 1003
BseBI CCWGG 2 cut(s) 419, 1181
BseDI CCNNGG 3 cut(s) 138, 417, 1180
BseGI GGATG 1 cut(s) 364
BseLI CCNNNNNNNGG 3 cut(s) 195, 341, 1130
BseMI GCAATG 1 cut(s) 1003
BseMII CTCAG 2 cut(s) 84, 1107
BseNI ACTGG 2 cut(s) 640, 1237
BseRI GAGGAG 2 cut(s) 1223, 1392
BseXI GCAGC 2 cut(s) 36, 1383
BsgI GTGCAG 3 cut(s) 453, 928, 1048
BshFI GGCC 1 cut(s) 764
BsiHKCI CYCGRG 1 cut(s) 15
BsiSI CCGG 1 cut(s) 1334
BslFI GGGAC 1 cut(s) 630
BslI CCNNNNNNNGG 3 cut(s) 195, 341, 1130
BsmAI GTCTC 1 cut(s) 544
BsmFI GGGAC 1 cut(s) 630
BsnI GGCC 1 cut(s) 764
Bso31I GGTCTC 1 cut(s) 544
BsoBI CYCGRG 1 cut(s) 15
Bsp143I GATC 3 cut(s) 142, 273, 1315
BspANI GGCC 1 cut(s) 764
BspCNI CTCAG 2 cut(s) 85, 1108
BspLI GGNNCC 3 cut(s) 275, 765, 1237
BspMI ACCTGC 1 cut(s) 991
BspOI GCTAGC 1 cut(s) 1055
BspPI GGATC 3 cut(s) 150, 268, 281
BspQI GCTCTTC 1 cut(s) 297
BspTNI GGTCTC 1 cut(s) 544
BsrDI GCAATG 1 cut(s) 1003
BsrI ACTGG 2 cut(s) 640, 1237
BssECI CCNNGG 3 cut(s) 138, 417, 1180
BssMI GATC 3 cut(s) 142, 273, 1315
BssT1I CCWWGG 1 cut(s) 138
Bst2UI CCWGG 2 cut(s) 419, 1181
Bst4CI ACNGT 4 cut(s) 242, 548, 668, 1355
Bst6I CTCTTC 1 cut(s) 297
BstC8I GCNNGC 2 cut(s) 1053, 1275
BstDEI CTNAG 3 cut(s) 93, 1116, 1200
BstEII GGTNACC 1 cut(s) 1213
BstF5I GGATG 1 cut(s) 364
BstKTI GATC 3 cut(s) 145, 276, 1318
BstMAI GTCTC 1 cut(s) 544
BstMBI GATC 3 cut(s) 142, 273, 1315
BstMWI GCNNNNNNNGC 1 cut(s) 915
BstNI CCWGG 2 cut(s) 419, 1181
BstNSI RCATGY 2 cut(s) 1267, 1277
BstPI GGTNACC 1 cut(s) 1213
BstSCI CCNGG 2 cut(s) 417, 1179
BstSFI CTRYAG 1 cut(s) 507
BstV1I GCAGC 2 cut(s) 36, 1383
BstX2I RGATCY 1 cut(s) 273
BstYI RGATCY 1 cut(s) 273
BsuRI GGCC 1 cut(s) 764
BtgZI GCGATG 1 cut(s) 344
BtsCI GGATG 1 cut(s) 364
BtsI GCAGTG 1 cut(s) 925
BtsIMutI CAGTG 4 cut(s) 238, 647, 925, 1230
BveI ACCTGC 1 cut(s) 991
Cac8I GCNNGC 2 cut(s) 1053, 1275
Cfr13I GGNCC 3 cut(s) 637, 763, 1236
Csp6I GTAC 6 cut(s) 287, 409, 1006, 1261, 1331, 1356
CviAII CATG 4 cut(s) 424, 1130, 1264, 1274
CviQI GTAC 6 cut(s) 287, 409, 1006, 1261, 1331, 1356
DdeI CTNAG 3 cut(s) 93, 1116, 1200
DpnI GATC 3 cut(s) 144, 275, 1317
DpnII GATC 3 cut(s) 142, 273, 1315
DrdI GACNNNNNNGTC 1 cut(s) 625
DseDI GACNNNNNNGTC 1 cut(s) 625
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 138
Eco31I GGTCTC 1 cut(s) 544
Eco47I GGWCC 2 cut(s) 637, 1236
Eco57I CTGAAG 2 cut(s) 338, 794
Eco88I CYCGRG 1 cut(s) 15
Eco91I GGTNACC 1 cut(s) 1213
EcoO65I GGTNACC 1 cut(s) 1213
EcoRI GAATTC 1 cut(s) 261
EcoRII CCWGG 2 cut(s) 417, 1179
EcoT14I CCWWGG 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 138
FaeI CATG 4 cut(s) 427, 1133, 1267, 1277
FalI AAGNNNNNCTT 4 cut(s) 604, 636, 918, 950
FaqI GGGAC 1 cut(s) 630
FatI CATG 4 cut(s) 423, 1129, 1263, 1273
Fnu4HI GCNGC 2 cut(s) 25, 1397
FokI GGATG 1 cut(s) 371
Fsp4HI GCNGC 2 cut(s) 25, 1397
FspBI CTAG 5 cut(s) 341, 938, 1052, 1205, 1368
GluI GCNGC 2 cut(s) 25, 1397
GsuI CTGGAG 1 cut(s) 1163
HaeIII GGCC 1 cut(s) 764
HapII CCGG 1 cut(s) 1334
Hin1II CATG 4 cut(s) 427, 1133, 1267, 1277
HinfI GANTC 5 cut(s) 95, 154, 398, 626, 722
HpaII CCGG 1 cut(s) 1334
HphI GGTGA 3 cut(s) 595, 695, 1151
Hpy166II GTNNAC 1 cut(s) 99
Hpy188I TCNGA 7 cut(s) 94, 205, 727, 774, 783, 858, 868
Hpy188III TCNNGA 8 cut(s) 151, 248, 258, 356, 440, 938, 1115, 1152
Hpy8I GTNNAC 1 cut(s) 99
Hpy99I CGWCG 1 cut(s) 1410
HpyAV CCTTC 2 cut(s) 387, 965
HpyCH4III ACNGT 4 cut(s) 242, 548, 668, 1355
HpyCH4IV ACGT 1 cut(s) 289
HpyCH4V TGCA 5 cut(s) 284, 434, 531, 909, 1029
HpyF10VI GCNNNNNNNGC 1 cut(s) 915
HpyF3I CTNAG 3 cut(s) 93, 1116, 1200
HpySE526I ACGT 1 cut(s) 289
Hsp92II CATG 4 cut(s) 427, 1133, 1267, 1277
Kzo9I GATC 3 cut(s) 142, 273, 1315
LguI GCTCTTC 1 cut(s) 297
Lsp1109I GCAGC 2 cut(s) 36, 1383
LweI GCATC 2 cut(s) 321, 823
MaeI CTAG 5 cut(s) 341, 938, 1052, 1205, 1368
MaeII ACGT 1 cut(s) 289
MaeIII GTNAC 6 cut(s) 10, 236, 502, 946, 1057, 1213
MalI GATC 3 cut(s) 144, 275, 1317
MboI GATC 3 cut(s) 142, 273, 1315
MboII GAAGA 7 cut(s) 314, 344, 524, 855, 923, 926, 1300
MflI RGATCY 1 cut(s) 273
MluCI AATT 9 cut(s) 73, 261, 324, 566, 690, 829, 1084, 1250, 1282
MlyI GAGTC 3 cut(s) 104, 407, 635
MmeI TCCRAC 2 cut(s) 251, 573
MseI TTAA 3 cut(s) 413, 924, 1414
MslI CAYNNNNRTG 1 cut(s) 1134
MspI CCGG 1 cut(s) 1334
MspR9I CCNGG 2 cut(s) 419, 1181
MvaI CCWGG 2 cut(s) 419, 1181
MwoI GCNNNNNNNGC 1 cut(s) 915
NdeII GATC 3 cut(s) 142, 273, 1315
NheI GCTAGC 1 cut(s) 1051
NlaIII CATG 4 cut(s) 427, 1133, 1267, 1277
NlaIV GGNNCC 3 cut(s) 275, 765, 1237
NmuCI GTSAC 3 cut(s) 236, 946, 1057
NspI RCATGY 2 cut(s) 1267, 1277
PaeI GCATGC 1 cut(s) 1277
PaeR7I CTCGAG 1 cut(s) 15
PaqCI CACCTGC 1 cut(s) 991
PciSI GCTCTTC 1 cut(s) 297
PfeI GAWTC 2 cut(s) 154, 722
PflMI CCANNNNNTGG 1 cut(s) 195
PkrI GCNGC 2 cut(s) 26, 1398
PleI GAGTC 3 cut(s) 103, 406, 634
PpsI GAGTC 3 cut(s) 103, 406, 634
Psp6I CCWGG 2 cut(s) 417, 1179
PspEI GGTNACC 1 cut(s) 1213
PspGI CCWGG 2 cut(s) 417, 1179
PspN4I GGNNCC 3 cut(s) 275, 765, 1237
PspPI GGNCC 3 cut(s) 637, 763, 1236
PspXI VCTCGAGB 1 cut(s) 15
PsrI GAACNNNNNNTAC 2 cut(s) 1339, 1371
PsuI RGATCY 1 cut(s) 273
RsaI GTAC 6 cut(s) 288, 410, 1007, 1262, 1332, 1357
RsaNI GTAC 6 cut(s) 287, 409, 1006, 1261, 1331, 1356
RseI CAYNNNNRTG 1 cut(s) 1134
SapI GCTCTTC 1 cut(s) 297
SaqAI TTAA 3 cut(s) 413, 924, 1414
SatI GCNGC 2 cut(s) 25, 1397
Sau3AI GATC 3 cut(s) 142, 273, 1315
Sau96I GGNCC 3 cut(s) 637, 763, 1236
SchI GAGTC 3 cut(s) 104, 407, 635
ScrFI CCNGG 2 cut(s) 419, 1181
SfaNI GCATC 2 cut(s) 321, 823
SfcI CTRYAG 1 cut(s) 507
Sfr274I CTCGAG 1 cut(s) 15
SinI GGWCC 2 cut(s) 637, 1236
SlaI CTCGAG 1 cut(s) 15
SmiMI CAYNNNNRTG 1 cut(s) 1134
SmlI CTYRAG 3 cut(s) 15, 248, 1268
SmoI CTYRAG 3 cut(s) 15, 248, 1268
SphI GCATGC 1 cut(s) 1277
Sse9I AATT 9 cut(s) 73, 261, 324, 566, 690, 829, 1084, 1250, 1282
SspMI CTAG 5 cut(s) 341, 938, 1052, 1205, 1368
StyD4I CCNGG 2 cut(s) 417, 1179
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 4 cut(s) 242, 548, 668, 1355
TaiI ACGT 1 cut(s) 292
TaqI TCGA 5 cut(s) 16, 78, 617, 657, 1408
TaqII GACCGA 1 cut(s) 625
TasI AATT 9 cut(s) 73, 261, 324, 566, 690, 829, 1084, 1250, 1282
TfiI GAWTC 2 cut(s) 154, 722
Tru1I TTAA 3 cut(s) 413, 924, 1414
Tru9I TTAA 3 cut(s) 413, 924, 1414
TscAI CASTG 4 cut(s) 245, 647, 925, 1237
TseFI GTSAC 3 cut(s) 236, 946, 1057
TseI GCWGC 2 cut(s) 24, 1396
Tsp45I GTSAC 3 cut(s) 236, 946, 1057
TspDTI ATGAA 4 cut(s) 41, 440, 842, 1118
TspGWI ACGGA 2 cut(s) 1357, 1405
TspRI CASTG 4 cut(s) 245, 647, 925, 1237
Van91I CCANNNNNTGG 1 cut(s) 195
VpaK11BI GGWCC 2 cut(s) 637, 1236
XapI RAATTY 2 cut(s) 73, 261
XbaI TCTAGA 1 cut(s) 937
XceI RCATGY 2 cut(s) 1267, 1277
XcmI CCANNNNNNNNNTGG 1 cut(s) 815
XhoI CTCGAG 1 cut(s) 15
XspI CTAG 5 cut(s) 341, 938, 1052, 1205, 1368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.