Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0003533

Physical Location & Seq

Genomic Coordinates Reverse (-)

40519816 .. 40523569

Gene Structure 3754 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000013765

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1590 bp

ATGGCCGCCGTCGCCGCCATTCGTCCCTTGGTCTCAGTCCAAAGCGTCGAAGGAGACATGGCCACCGATCAGGCCCACACCGTGGCCTTAGCCGACGTCATGAAGGCCTCGATCCGACCCGACATCGTCACCTTCGTCCACTCCAACATTTCCAAGAACAGCCGCCAGGCCTACGCCGTCTCCAAGAAGGCCGGCCACCAGACCTCCGCCGAGTCCTGGGGTACCGGCCGCGCCGTCTCCCGTATCCCCCGTGTCCCCGGAGGCGGCACCCACCGCGCCGGCCAGGCTGCCTTCGGCAACATGTGCCGCGGCGGCCGCATGTTCGCCCCCACCAAGATCTGGCGCCGCTGGCACCGCAAGATCAACGTCAACCAGAAGCGGTACGCCATCGTCTCCGCCATCGCCGCTTCCTCGGTTCCGTCTCTGGTCACCGCCAGGGGTCACAAGATCGAGAATGTTCCCGAATTGCCCCTGGTCATCAGCGACACCGCCGAGGGCGTCGAGAAGACCTCCGCGGCTATCAAGCTGCTGAAGCAGATCGGGGCCTATGATGATGCTGAGAAGGCCAAGGACAGCCACTCGATTCGGCCCGGGAAGGGGAAGATGAGGAACCGCCGGTACATCAACCGCAAGGGGCCGCTCATTGTTTACGGCACCGAGGGGGCTAAGCTCGTCAATGCCTTCAGGAACATTCCCGGAGTTGATATCATCAATGTGGAGAGGCTCAATCTGCTGAAGCTTGCACCTGGTGGCCATCTCGGCCGGTTTGTTATCTGGACCAAGTGTGCTTTTGAGAAGCTGGACTCGATTTACGGGTCGTTTGATAAGCCTTCGGAGAAGAAGAAGGGGTATGTGCTGCCCAGGGCTAAGATGGTCAATGCTGACCTTGCTAGGATTATCAACTCCGATGAGGTTCAGTCTGTTGTTAGGCCCATCAAGAGGGAGGTGAAGAGGGCACCACTGAAGAAGAACCCGCTCAAGAATCTCAACACCATGCTCAGGCTTAACCCGTATGCTAAGACTGCCAAGAGAATGGCGCTGTTGGCTGAGGAGCAGAGGCTTAAGTCCAAGAAGGAGAAGCTTGACAGGAAGAGGAAGCCCATTTCAAAGGAGGAAGCTTCTACCATCAAGGCAGCTGGAAAAGCATGGTACCAAACAATGATCTCCGACAGTGATTACACCGAGTTTGACGTCTTCCAGAAGTGGCTTGGTGTATCCCAAACCACGAGACTGGGCGAGGTCGTGCTCCACAATCGCATCCAAAAAGAGGAGCATGGAGTTGACGCTAGACTGGGCGAGGTCGAGCTCGCGGACCTTAGCGCTGAAGTGGTCGGTGAGGTCGCAAGTGGAGGAGACGTTGGAGAGGACGTGGTCGGCGTCGGACTTGATGATGTCGAGGACATGGGAGGATTTATGGAGATTGGAGAGGTCGCGGATCTTGGCGCTGACGTGGTTGGCGAGGTCGCAGGTGAAGGAGACGTTGGAGAGGATGTGGTCGGCGTCGGACTTGACGATGTCGAGGACCTGGGAGGATTTCTGGAGATTGGAGAGGTGCTTGTCGAGGTCGGAGCGCTAGAAGAGAGGAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002181 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0005911 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009507 GO:0009536 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010467 GO:0015934 GO:0016020 GO:0019538 GO:0022625 GO:0022626 GO:0030054 GO:0030312 GO:0031090 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0034645 GO:0042221 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0050896 GO:0055044 GO:0070013 GO:0071704 GO:0071944 GO:0097159 GO:0098588 GO:0098805 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03010 map03010

Pfam Domains

Protein Families

Ribos_L4_asso_C Ribosomal_L4

Protein Analysis

530

Amino Acids

57.61

Weight (kDa)

9.23

Isoelectric Point (pI)

30.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ribosomal_L4	PF00573	30 - 269	5e-34	Ribosomal protein L4/L1 family
Ribos_L4_asso_C	PF14374	282 - 355	6.6e-29	60S ribosomal protein L4 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003533)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09630 AT3G09630 AT5G02870 AT5G02870
fragaria_vesca	FvH4_4g07470 FvH4_6g15170 FvH4_6g15170 FvH4_6g15170
malus_domestica	MD12G1126000.v1.1 MD16G1251600.v1.1
prunus_persica	Prupe.1G078100_v2.0.a1 Prupe.1G078100_v2.0.a1 Prupe.6G242600_v2.0.a1
pyrus_communis	pycom04g10060 pycom12g12420 pycom16g21150
rosa_chinensis	RchiOBHm_Chr3g0468471 RchiOBHm_Chr6g0270851
rosa_laevigata	RLG00000013765 RLG00000024426
rosa_multiflora	Rmu_sc0000642.1_g000029 Rmu_sc0008735.1_g000002 Rmu_sc0008735.1_g000003
rosa_roxburghii	Rroxscaffold_6G00412730 Rroxscaffold_7G00197580
rosa_rugosa	Rorug03G0095700 Rorug06G0056900
rosa_samantha	Rh3AG143500 Rh3BG166000 Rh6AG176200 Rh6BG179400 Rh6CG175600 Rh6DG167900
rosa_wichuraiana	Rw3G013590 Rw6G015010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1457
AatII	GACGTC	2 cut(s)	99, 1194
Acc36I	ACCTGC	1 cut(s)	1457
Acc65I	GGTACC	2 cut(s)	221, 1149
AccB1I	GGYRCC	7 cut(s)	221, 266, 342, 351, 653, 955, 1149
AccB7I	CCANNNNNTGG	2 cut(s)	82, 339
AccBSI	CCGCTC	2 cut(s)	640, 976
AccII	CGCG	6 cut(s)	231, 276, 309, 515, 1310, 1433
AclWI	GGATC	2 cut(s)	106, 1443
AcoI	YGGCCR	8 cut(s)	3, 60, 193, 226, 280, 313, 751, 760
AcuI	CTGAAG	5 cut(s)	551, 667, 755, 983, 1344
AcyI	GRCGYC	6 cut(s)	96, 343, 498, 1191, 1377, 1500
AdeI	CACNNNGTG	2 cut(s)	82, 749
AfaI	GTAC	4 cut(s)	223, 383, 620, 1151
AfeI	AGCGCT	2 cut(s)	1321, 1572
AfiI	CCNNNNNNNGG	9 cut(s)	82, 216, 263, 339, 596, 597, 939, 999, 1267
AflII	CTTAAG	1 cut(s)	1061
AflIII	ACRYGT	1 cut(s)	300
AgsI	TTSAA	1 cut(s)	1107
AjiI	CACGTC	2 cut(s)	1369, 1450
AjnI	CCWGG	8 cut(s)	165, 215, 282, 434, 471, 745, 860, 1524
AjuI	GAANNNNNNNTTGG	2 cut(s)	1464, 1496
AluBI	AGCT	8 cut(s)	526, 670, 739, 799, 1081, 1118, 1136, 1306
AluI	AGCT	8 cut(s)	526, 670, 739, 799, 1081, 1118, 1136, 1306
Alw21I	GWGCWC	2 cut(s)	1248, 1308
Alw26I	GTCTC	9 cut(s)	37, 48, 184, 241, 397, 426, 1222, 1347, 1470
AlwI	GGATC	2 cut(s)	106, 1443
Ama87I	CYCGRG	1 cut(s)	590
Aor51HI	AGCGCT	2 cut(s)	1321, 1572
ApeKI	GCWGC	4 cut(s)	287, 526, 856, 1133
Asp718I	GGTACC	2 cut(s)	221, 1149
AspLEI	GCGC	7 cut(s)	233, 278, 345, 1039, 1322, 1445, 1573
AspS9I	GGNCC	8 cut(s)	73, 543, 588, 635, 777, 930, 1312, 1522
AsuC2I	CCSGG	4 cut(s)	258, 591, 592, 696
AsuHPI	GGTGA	5 cut(s)	121, 421, 958, 1346, 1481
AvaI	CYCGRG	1 cut(s)	590
AvaII	GGWCC	3 cut(s)	777, 1312, 1522
BaeGI	GKGCMC	1 cut(s)	958
BalI	TGGCCA	2 cut(s)	62, 753
BanI	GGYRCC	7 cut(s)	221, 266, 342, 351, 653, 955, 1149
BanII	GRGCYC	1 cut(s)	1308
BauI	CACGAG	1 cut(s)	1225
BbsI	GAAGAC	2 cut(s)	512, 1186
Bbv12I	GWGCWC	2 cut(s)	1248, 1308
BbvCI	CCTCAGC	1 cut(s)	1047
BbvI	GCAGC	4 cut(s)	274, 513, 843, 1145
BccI	CCATC	6 cut(s)	395, 407, 762, 865, 941, 1133
BceAI	ACGGC	3 cut(s)	161, 218, 667
BciT130I	CCWGG	8 cut(s)	167, 217, 284, 436, 473, 747, 862, 1526
BciVI	GTATCC	2 cut(s)	254, 1225
BcnI	CCSGG	4 cut(s)	258, 591, 592, 696
BcoDI	GTCTC	9 cut(s)	37, 48, 184, 241, 397, 426, 1222, 1347, 1470
BfaI	CTAG	3 cut(s)	891, 1287, 1574
BfoI	RGCGCY	5 cut(s)	346, 1040, 1323, 1446, 1574
BfrI	CTTAAG	1 cut(s)	1061
BfuAI	ACCTGC	1 cut(s)	1457
BfuI	GTATCC	2 cut(s)	254, 1225
BglI	GCCNNNNNGGC	3 cut(s)	284, 312, 759
BglII	AGATCT	1 cut(s)	336
BlpI	GCTNAGC	1 cut(s)	666
Bme18I	GGWCC	3 cut(s)	777, 1312, 1522
BmeT110I	CYCGRG	1 cut(s)	590
BmgBI	CACGTC	2 cut(s)	1369, 1450
BmgT120I	GGNCC	8 cut(s)	73, 543, 588, 635, 777, 930, 1312, 1522
BmrI	ACTGGG	2 cut(s)	1241, 1301
BmsI	GCATC	2 cut(s)	544, 1266
BmuI	ACTGGG	2 cut(s)	1241, 1301
BpiI	GAAGAC	2 cut(s)	512, 1186
BplI	GAGNNNNNCTC	2 cut(s)	494, 526
BpmI	CTGGAG	1 cut(s)	1559
Bpu10I	CCTNAGC	4 cut(s)	88, 998, 1047, 1316
Bpu1102I	GCTNAGC	1 cut(s)	666
BpuEI	CTTGAG	1 cut(s)	962
BpuMI	CCSGG	4 cut(s)	258, 591, 592, 696
BsaHI	GRCGYC	6 cut(s)	96, 343, 498, 1191, 1377, 1500
BsaI	GGTCTC	1 cut(s)	37
Bsc4I	CCNNNNNNNGG	9 cut(s)	82, 216, 263, 339, 596, 597, 939, 999, 1267
Bse118I	RCCGGY	5 cut(s)	191, 224, 278, 615, 762
Bse1I	ACTGG	2 cut(s)	1236, 1296
BseBI	CCWGG	8 cut(s)	167, 217, 284, 436, 473, 747, 862, 1526
BseGI	GGATG	2 cut(s)	1257, 1495
BseLI	CCNNNNNNNGG	9 cut(s)	82, 216, 263, 339, 596, 597, 939, 999, 1267
BseMII	CTCAG	4 cut(s)	48, 549, 1012, 1038
BseNI	ACTGG	2 cut(s)	1236, 1296
BseRI	GAGGAG	3 cut(s)	1064, 1283, 1365
BseSI	GKGCMC	1 cut(s)	958
BseX3I	CGGCCG	3 cut(s)	226, 313, 760
BseXI	GCAGC	4 cut(s)	274, 513, 843, 1145
Bsh1236I	CGCG	6 cut(s)	231, 276, 309, 515, 1310, 1433
Bsh1285I	CGRYCG	3 cut(s)	229, 316, 763
BshNI	GGYRCC	7 cut(s)	221, 266, 342, 351, 653, 955, 1149
BsiEI	CGRYCG	3 cut(s)	229, 316, 763
BsiHKAI	GWGCWC	2 cut(s)	1248, 1308
BsiHKCI	CYCGRG	1 cut(s)	590
BsiSI	CCGG	8 cut(s)	192, 225, 258, 279, 591, 616, 696, 763
BslFI	GGGAC	2 cut(s)	9, 239
BslI	CCNNNNNNNGG	9 cut(s)	82, 216, 263, 339, 596, 597, 939, 999, 1267
BsmAI	GTCTC	9 cut(s)	37, 48, 184, 241, 397, 426, 1222, 1347, 1470
BsmBI	CGTCTC	6 cut(s)	184, 241, 397, 426, 1347, 1470
BsmFI	GGGAC	2 cut(s)	9, 239
Bso31I	GGTCTC	1 cut(s)	37
BsoBI	CYCGRG	1 cut(s)	590
Bsp1286I	GDGCHC	3 cut(s)	958, 1248, 1308
Bsp143I	GATC	8 cut(s)	67, 111, 336, 360, 447, 537, 1161, 1435
Bsp1720I	GCTNAGC	1 cut(s)	666
BspCNI	CTCAG	4 cut(s)	47, 550, 1011, 1039
BspFNI	CGCG	6 cut(s)	231, 276, 309, 515, 1310, 1433
BspHI	TCATGA	1 cut(s)	99
BspMI	ACCTGC	1 cut(s)	1457
BspPI	GGATC	2 cut(s)	106, 1443
BspT107I	GGYRCC	7 cut(s)	221, 266, 342, 351, 653, 955, 1149
BspTI	CTTAAG	1 cut(s)	1061
BspTNI	GGTCTC	1 cut(s)	37
BsrBI	CCGCTC	2 cut(s)	640, 976
BsrFI	RCCGGY	5 cut(s)	191, 224, 278, 615, 762
BsrI	ACTGG	2 cut(s)	1236, 1296
BssAI	RCCGGY	5 cut(s)	191, 224, 278, 615, 762
BssMI	GATC	8 cut(s)	67, 111, 336, 360, 447, 537, 1161, 1435
BssNI	GRCGYC	6 cut(s)	96, 343, 498, 1191, 1377, 1500
BssSI	CACGAG	1 cut(s)	1225
BssT1I	CCWWGG	2 cut(s)	27, 567
Bst2BI	CACGAG	1 cut(s)	1225
Bst2UI	CCWGG	8 cut(s)	167, 217, 284, 436, 473, 747, 862, 1526
Bst4CI	ACNGT	2 cut(s)	82, 1172
Bst6I	CTCTTC	3 cut(s)	944, 1085, 1572
BstACI	GRCGYC	6 cut(s)	96, 343, 498, 1191, 1377, 1500
BstAFI	CTTAAG	1 cut(s)	1061
BstAPI	GCANNNNNTGC	1 cut(s)	303
BstC8I	GCNNGC	5 cut(s)	193, 280, 350, 741, 1308
BstDEI	CTNAG	9 cut(s)	34, 88, 558, 666, 867, 998, 1017, 1047, 1316
BstDSI	CCRYGG	3 cut(s)	81, 307, 513
BstEII	GGTNACC	1 cut(s)	427
BstF5I	GGATG	2 cut(s)	1257, 1495
BstFNI	CGCG	6 cut(s)	231, 276, 309, 515, 1310, 1433
BstH2I	RGCGCY	5 cut(s)	346, 1040, 1323, 1446, 1574
BstHHI	GCGC	7 cut(s)	233, 278, 345, 1039, 1322, 1445, 1573
BstKTI	GATC	8 cut(s)	70, 114, 339, 363, 450, 540, 1164, 1438
BstMAI	GTCTC	9 cut(s)	37, 48, 184, 241, 397, 426, 1222, 1347, 1470
BstMBI	GATC	8 cut(s)	67, 111, 336, 360, 447, 537, 1161, 1435
BstMCI	CGRYCG	3 cut(s)	229, 316, 763
BstNI	CCWGG	8 cut(s)	167, 217, 284, 436, 473, 747, 862, 1526
BstNSI	RCATGY	2 cut(s)	304, 322
BstPI	GGTNACC	1 cut(s)	427
BstSLI	GKGCMC	1 cut(s)	958
BstUI	CGCG	6 cut(s)	231, 276, 309, 515, 1310, 1433
BstV1I	GCAGC	4 cut(s)	274, 513, 843, 1145
BstV2I	GAAGAC	2 cut(s)	512, 1186
BstX2I	RGATCY	2 cut(s)	336, 1435
BstXI	CCANNNNNNTGG	2 cut(s)	1033, 1231
BstYI	RGATCY	2 cut(s)	336, 1435
BstZI	CGGCCG	3 cut(s)	226, 313, 760
BsuI	GTATCC	2 cut(s)	254, 1225
BtgI	CCRYGG	3 cut(s)	81, 307, 513
BtgZI	GCGATG	1 cut(s)	385
BtrI	CACGTC	2 cut(s)	1369, 1450
BtsCI	GGATG	2 cut(s)	1257, 1495
BtsIMutI	CAGTG	2 cut(s)	959, 1177
BveI	ACCTGC	1 cut(s)	1457
Cac8I	GCNNGC	5 cut(s)	193, 280, 350, 741, 1308
CciI	TCATGA	1 cut(s)	99
CciNI	GCGGCCGC	1 cut(s)	313
CfoI	GCGC	7 cut(s)	233, 278, 345, 1039, 1322, 1445, 1573
Cfr10I	RCCGGY	5 cut(s)	191, 224, 278, 615, 762
Cfr13I	GGNCC	8 cut(s)	73, 543, 588, 635, 777, 930, 1312, 1522
Cfr42I	CCGCGG	2 cut(s)	310, 516
Cfr9I	CCCGGG	1 cut(s)	590
CseI	GACGC	5 cut(s)	34, 487, 1292, 1366, 1489
CsiI	ACCWGGT	1 cut(s)	745
Csp6I	GTAC	4 cut(s)	222, 382, 619, 1150
CviAII	CATG	8 cut(s)	58, 100, 301, 319, 994, 1146, 1274, 1402
CviQI	GTAC	4 cut(s)	222, 382, 619, 1150
DdeI	CTNAG	9 cut(s)	34, 88, 558, 666, 867, 998, 1017, 1047, 1316
DinI	GGCGCC	1 cut(s)	344
DpnI	GATC	8 cut(s)	69, 113, 338, 362, 449, 539, 1163, 1437
DpnII	GATC	8 cut(s)	67, 111, 336, 360, 447, 537, 1161, 1435
DraIII	CACNNNGTG	2 cut(s)	82, 749
EaeI	YGGCCR	8 cut(s)	3, 60, 193, 226, 280, 313, 751, 760
EagI	CGGCCG	3 cut(s)	226, 313, 760
Eam1104I	CTCTTC	3 cut(s)	944, 1085, 1572
EarI	CTCTTC	3 cut(s)	944, 1085, 1572
EciI	GGCGGA	2 cut(s)	196, 385
Ecl136II	GAGCTC	1 cut(s)	1306
EclXI	CGGCCG	3 cut(s)	226, 313, 760
Eco130I	CCWWGG	2 cut(s)	27, 567
Eco147I	AGGCCT	2 cut(s)	107, 170
Eco24I	GRGCYC	1 cut(s)	1308
Eco31I	GGTCTC	1 cut(s)	37
Eco32I	GATATC	1 cut(s)	706
Eco47I	GGWCC	3 cut(s)	777, 1312, 1522
Eco47III	AGCGCT	2 cut(s)	1321, 1572
Eco52I	CGGCCG	3 cut(s)	226, 313, 760
Eco53kI	GAGCTC	1 cut(s)	1306
Eco57I	CTGAAG	5 cut(s)	551, 667, 755, 983, 1344
Eco88I	CYCGRG	1 cut(s)	590
Eco91I	GGTNACC	1 cut(s)	427
EcoICRI	GAGCTC	1 cut(s)	1306
EcoO109I	RGGNCCY	2 cut(s)	543, 1522
EcoO65I	GGTNACC	1 cut(s)	427
EcoRII	CCWGG	8 cut(s)	165, 215, 282, 434, 471, 745, 860, 1524
EcoRV	GATATC	1 cut(s)	706
EcoT14I	CCWWGG	2 cut(s)	27, 567
EcoT38I	GRGCYC	1 cut(s)	1308
EgeI	GGCGCC	1 cut(s)	344
EheI	GGCGCC	1 cut(s)	344
ErhI	CCWWGG	2 cut(s)	27, 567
Esp3I	CGTCTC	6 cut(s)	184, 241, 397, 426, 1347, 1470
FaeI	CATG	8 cut(s)	61, 103, 304, 322, 997, 1149, 1277, 1405
FaqI	GGGAC	2 cut(s)	9, 239
FatI	CATG	8 cut(s)	57, 99, 300, 318, 993, 1145, 1273, 1401
FauI	CCCGC	1 cut(s)	981
FokI	GGATG	2 cut(s)	1244, 1502
FriOI	GRGCYC	1 cut(s)	1308
FseI	GGCCGGCC	1 cut(s)	195
FspBI	CTAG	3 cut(s)	891, 1287, 1574
GlaI	GCGC	7 cut(s)	232, 277, 344, 1038, 1321, 1444, 1572
GsuI	CTGGAG	1 cut(s)	1559
HaeII	RGCGCY	5 cut(s)	346, 1040, 1323, 1446, 1574
HapII	CCGG	8 cut(s)	192, 225, 258, 279, 591, 616, 696, 763
HgaI	GACGC	5 cut(s)	34, 487, 1292, 1366, 1489
HhaI	GCGC	7 cut(s)	233, 278, 345, 1039, 1322, 1445, 1573
Hin1I	GRCGYC	6 cut(s)	96, 343, 498, 1191, 1377, 1500
Hin1II	CATG	8 cut(s)	61, 103, 304, 322, 997, 1149, 1277, 1405
Hin6I	GCGC	7 cut(s)	231, 276, 343, 1037, 1320, 1443, 1571
HinP1I	GCGC	7 cut(s)	231, 276, 343, 1037, 1320, 1443, 1571
HincII	GTYRAC	2 cut(s)	370, 1282
HindII	GTYRAC	2 cut(s)	370, 1282
HindIII	AAGCTT	3 cut(s)	737, 1079, 1116
HinfI	GANTC	4 cut(s)	212, 583, 803, 982
HpaII	CCGG	8 cut(s)	192, 225, 258, 279, 591, 616, 696, 763
HphI	GGTGA	5 cut(s)	121, 421, 958, 1346, 1481
Hpy166II	GTNNAC	4 cut(s)	139, 370, 649, 1282
Hpy188I	TCNGA	7 cut(s)	116, 835, 907, 1168, 1382, 1505, 1568
Hpy8I	GTNNAC	4 cut(s)	139, 370, 649, 1282
Hpy99I	CGWCG	6 cut(s)	14, 50, 98, 503, 1382, 1505
HpyCH4III	ACNGT	2 cut(s)	82, 1172
HpyCH4IV	ACGT	7 cut(s)	96, 366, 1191, 1356, 1368, 1449, 1479
HpyCH4V	TGCA	1 cut(s)	743
HpyF3I	CTNAG	9 cut(s)	34, 88, 558, 666, 867, 998, 1017, 1047, 1316
HpySE526I	ACGT	7 cut(s)	96, 366, 1191, 1356, 1368, 1449, 1479
Hsp92I	GRCGYC	6 cut(s)	96, 343, 498, 1191, 1377, 1500
Hsp92II	CATG	8 cut(s)	61, 103, 304, 322, 997, 1149, 1277, 1405
HspAI	GCGC	7 cut(s)	231, 276, 343, 1037, 1320, 1443, 1571
KasI	GGCGCC	1 cut(s)	342
KpnI	GGTACC	2 cut(s)	225, 1153
KroI	GCCGGC	2 cut(s)	191, 278
KroNI	GCCGGC	2 cut(s)	193, 280
KspI	CCGCGG	2 cut(s)	310, 516
Kzo9I	GATC	8 cut(s)	67, 111, 336, 360, 447, 537, 1161, 1435
LmnI	GCTCC	4 cut(s)	1051, 1251, 1270, 1568
Lsp1109I	GCAGC	4 cut(s)	274, 513, 843, 1145
LweI	GCATC	2 cut(s)	544, 1266
MabI	ACCWGGT	1 cut(s)	745
MaeI	CTAG	3 cut(s)	891, 1287, 1574
MaeII	ACGT	7 cut(s)	96, 366, 1191, 1356, 1368, 1449, 1479
MaeIII	GTNAC	3 cut(s)	127, 427, 440
MalI	GATC	8 cut(s)	69, 113, 338, 362, 449, 539, 1163, 1437
MbiI	CCGCTC	2 cut(s)	640, 976
MboI	GATC	8 cut(s)	67, 111, 336, 360, 447, 537, 1161, 1435
MflI	RGATCY	2 cut(s)	336, 1435
MhlI	GDGCHC	3 cut(s)	958, 1248, 1308
MlsI	TGGCCA	2 cut(s)	62, 753
MluCI	AATT	1 cut(s)	464
MluNI	TGGCCA	2 cut(s)	62, 753
Mly113I	GGCGCC	1 cut(s)	343
MlyI	GAGTC	2 cut(s)	221, 797
MmeI	TCCRAC	8 cut(s)	139, 168, 1191, 1339, 1360, 1462, 1483, 1546
Mox20I	TGGCCA	2 cut(s)	62, 753
MroNI	GCCGGC	2 cut(s)	191, 278
MscI	TGGCCA	2 cut(s)	62, 753
MseI	TTAA	2 cut(s)	1005, 1062
MslI	CAYNNNNRTG	1 cut(s)	713
Msp20I	TGGCCA	2 cut(s)	62, 753
MspA1I	CMGCKG	4 cut(s)	309, 348, 515, 1136
MspCI	CTTAAG	1 cut(s)	1061
MspI	CCGG	8 cut(s)	192, 225, 258, 279, 591, 616, 696, 763
MvaI	CCWGG	8 cut(s)	167, 217, 284, 436, 473, 747, 862, 1526
MvnI	CGCG	6 cut(s)	231, 276, 309, 515, 1310, 1433
NaeI	GCCGGC	2 cut(s)	193, 280
NarI	GGCGCC	1 cut(s)	343
NciI	CCSGG	4 cut(s)	258, 591, 592, 696
NdeII	GATC	8 cut(s)	67, 111, 336, 360, 447, 537, 1161, 1435
NgoMIV	GCCGGC	2 cut(s)	191, 278
NlaIII	CATG	8 cut(s)	61, 103, 304, 322, 997, 1149, 1277, 1405
NmeAIII	GCCGAG	3 cut(s)	235, 517, 738
NmuCI	GTSAC	3 cut(s)	127, 427, 440
NotI	GCGGCCGC	1 cut(s)	313
NspI	RCATGY	2 cut(s)	304, 322
PagI	TCATGA	1 cut(s)	99
PaqCI	CACCTGC	1 cut(s)	1457
PasI	CCCWGGG	1 cut(s)	861
PceI	AGGCCT	2 cut(s)	107, 170
PciI	ACATGT	1 cut(s)	300
PcsI	WCGNNNNNNNCGW	4 cut(s)	132, 1374, 1455, 1509
PdiI	GCCGGC	2 cut(s)	193, 280
PfeI	GAWTC	2 cut(s)	583, 982
PflFI	GACNNNGTC	3 cut(s)	125, 1370, 1514
PflMI	CCANNNNNTGG	2 cut(s)	82, 339
PfoI	TCCNGGA	1 cut(s)	694
PleI	GAGTC	2 cut(s)	220, 797
PluTI	GGCGCC	1 cut(s)	346
PpsI	GAGTC	2 cut(s)	220, 797
PpuMI	RGGWCCY	1 cut(s)	1522
PscI	ACATGT	1 cut(s)	300
Psp124BI	GAGCTC	1 cut(s)	1308
Psp5II	RGGWCCY	1 cut(s)	1522
Psp6I	CCWGG	8 cut(s)	165, 215, 282, 434, 471, 745, 860, 1524
PspEI	GGTNACC	1 cut(s)	427
PspGI	CCWGG	8 cut(s)	165, 215, 282, 434, 471, 745, 860, 1524
PspPI	GGNCC	8 cut(s)	73, 543, 588, 635, 777, 930, 1312, 1522
PspPPI	RGGWCCY	1 cut(s)	1522
PsrI	GAACNNNNNNTAC	2 cut(s)	602, 634
PsuI	RGATCY	2 cut(s)	336, 1435
PsyI	GACNNNGTC	3 cut(s)	125, 1370, 1514
PvuII	CAGCTG	1 cut(s)	1136
RigI	GGCCGGCC	1 cut(s)	195
RsaI	GTAC	4 cut(s)	223, 383, 620, 1151
RsaNI	GTAC	4 cut(s)	222, 382, 619, 1150
RseI	CAYNNNNRTG	1 cut(s)	713
SacI	GAGCTC	1 cut(s)	1308
SacII	CCGCGG	2 cut(s)	310, 516
SaqAI	TTAA	2 cut(s)	1005, 1062
Sau3AI	GATC	8 cut(s)	67, 111, 336, 360, 447, 537, 1161, 1435
Sau96I	GGNCC	8 cut(s)	73, 543, 588, 635, 777, 930, 1312, 1522
SchI	GAGTC	2 cut(s)	221, 797
SduI	GDGCHC	3 cut(s)	958, 1248, 1308
SexAI	ACCWGGT	1 cut(s)	745
SfaNI	GCATC	2 cut(s)	544, 1266
SfiI	GGCCNNNNNGGCC	1 cut(s)	759
SfoI	GGCGCC	1 cut(s)	344
Sfr303I	CCGCGG	2 cut(s)	310, 516
SgrBI	CCGCGG	2 cut(s)	310, 516
SinI	GGWCC	3 cut(s)	777, 1312, 1522
SmaI	CCCGGG	1 cut(s)	592
SmiMI	CAYNNNNRTG	1 cut(s)	713
SmlI	CTYRAG	2 cut(s)	977, 1061
SmoI	CTYRAG	2 cut(s)	977, 1061
Sse9I	AATT	1 cut(s)	464
SseBI	AGGCCT	2 cut(s)	107, 170
SspDI	GGCGCC	1 cut(s)	342
SspMI	CTAG	3 cut(s)	891, 1287, 1574
SstI	GAGCTC	1 cut(s)	1308
StuI	AGGCCT	2 cut(s)	107, 170
StyI	CCWWGG	2 cut(s)	27, 567
TaaI	ACNGT	2 cut(s)	82, 1172
TaiI	ACGT	7 cut(s)	99, 369, 1194, 1359, 1371, 1452, 1482
TasI	AATT	1 cut(s)	464
TfiI	GAWTC	2 cut(s)	583, 982
Tru1I	TTAA	2 cut(s)	1005, 1062
Tru9I	TTAA	2 cut(s)	1005, 1062
TscAI	CASTG	2 cut(s)	966, 1177
TseFI	GTSAC	3 cut(s)	127, 427, 440
TseI	GCWGC	4 cut(s)	287, 526, 856, 1133
Tsp45I	GTSAC	3 cut(s)	127, 427, 440
TspDTI	ATGAA	1 cut(s)	116
TspGWI	ACGGA	1 cut(s)	408
TspMI	CCCGGG	1 cut(s)	590
TspRI	CASTG	2 cut(s)	966, 1177
Tth111I	GACNNNGTC	3 cut(s)	125, 1370, 1514
Van91I	CCANNNNNTGG	2 cut(s)	82, 339
Vha464I	CTTAAG	1 cut(s)	1061
VpaK11BI	GGWCC	3 cut(s)	777, 1312, 1522
XceI	RCATGY	2 cut(s)	304, 322
XcmI	CCANNNNNNNNNTGG	3 cut(s)	25, 868, 1205
XmaI	CCCGGG	1 cut(s)	590
XspI	CTAG	3 cut(s)	891, 1287, 1574
ZraI	GACGTC	2 cut(s)	97, 1192

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.