RLG00000015225

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0012696
Physical Location & Seq
Forward (+)
63324432 .. 63326204
1773 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015225

Sequence Viewer

Length: 936 bp
ATGTCCACATTCATAAATGTACATAGAGAAACCGAGATCTTCCTGAAGCTTACAATTGACCGAGAGAACAGAAAGGTATTGTTTGCAGAAGCAAACAAGGATTTTGTTGACAATCTGGTGGGATTCATGATTTTCCCTACCTCTGCCATGATCACGCTTAACCCTGAACCTTTTGGAAAGCTAAACAATGTCAAGAAGAGCATTGAGAACACGACCGAGTATTACAATCCAATGAAGGCTTCTCTCCTGAATACAAAGCCACCAGCTCACTGCAATCGGCACGAGCATGCCCTGCATCCCCTTGATCCCTCATCACTTAAGAAGATGCCCTTTTATGTATGTGAAGAGTGTATTACTACCCGAGGTAAGCCATACTACGTGGCCGACGATCCCGCAGTCTTGTGTCGTACTTGTAACCATGCCATGACTCAGGGTGCAAGTTATTGTGGTACACCTATTGAAATCGCCAATGAAGGGGGAGGTTTTGTTCGACAGCTAGCGAAATACATCATTACTGATGATTTGAATATTCACCCTCTTCCCACGGCTTCTCTCACCACAAACATAATTCTTGGAGCTAAACCTCAGCATGTTGAAGAGGTTACCGTGAACGTTTCTCATAAGAAGTTGGTGTTGATGGGGGTGGTGGTGGTGGTGGATGTGTTCTATAGTGGCTGCGGGTTGTCCCGATCGGTCTTTGAAGGGGGTGCTGGTGGATTTGGCATGCTTGGCATCGACGCTCGTGACAGCATTGGCATGGAAGACGACCAATCTCAGGTCGGGTTTTGTAGTGGTGATACTATTGCTGGTTGTCATGTCGGATTTGCGGCTGGAATGGACGATGGAGGTGAGGTCAGTGGTGGCGACTTTACAAGGTCTGCGATCGTCAGGAGTGCTAGGGTTTCCCTAGGTTCTGGTTTTTGGGCCCTTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

312

Amino Acids

33.66

Weight (kDa)

5.8

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 10 - 210 3.2e-16 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 393, 678, 827
AclI AACGTT 1 cut(s) 612
AclWI GGATC 2 cut(s) 299, 383
AcoI YGGCCR 1 cut(s) 381
AcuI CTGAAG 1 cut(s) 65
AfaI GTAC 3 cut(s) 21, 409, 451
AfiI CCNNNNNNNGG 2 cut(s) 474, 775
AflII CTTAAG 1 cut(s) 317
AgsI TTSAA 4 cut(s) 461, 526, 596, 701
AjuI GAANNNNNNNTTGG 2 cut(s) 223, 255
AloI GAACNNNNNNTCC 2 cut(s) 471, 503
AluBI AGCT 5 cut(s) 49, 181, 266, 496, 578
AluI AGCT 5 cut(s) 49, 181, 266, 496, 578
AlwI GGATC 2 cut(s) 299, 383
Ama87I CYCGRG 1 cut(s) 360
AoxI GGCC 2 cut(s) 381, 924
ApaI GGGCCC 1 cut(s) 928
ApeKI GCWGC 1 cut(s) 675
AspA2I CCTAGG 1 cut(s) 907
AspS9I GGNCC 2 cut(s) 924, 925
AsuHPI GGTGA 4 cut(s) 524, 547, 806, 860
AsuNHI GCTAGC 1 cut(s) 496
AvaI CYCGRG 1 cut(s) 360
AvrII CCTAGG 1 cut(s) 907
BaeGI GKGCMC 1 cut(s) 928
BanII GRGCYC 1 cut(s) 928
BauI CACGAG 2 cut(s) 281, 741
BbsI GAAGAC 1 cut(s) 768
BbvCI CCTCAGC 1 cut(s) 585
BbvI GCAGC 1 cut(s) 662
BccI CCATC 2 cut(s) 631, 836
BceAI ACGGC 1 cut(s) 561
BclI TGATCA 1 cut(s) 150
BfaI CTAG 3 cut(s) 497, 897, 908
BfmI CTRYAG 1 cut(s) 667
BfrI CTTAAG 1 cut(s) 317
BglII AGATCT 1 cut(s) 36
BisI GCNGC 2 cut(s) 676, 828
BlnI CCTAGG 1 cut(s) 907
BlsI GCNGC 2 cut(s) 677, 829
BmeT110I CYCGRG 1 cut(s) 360
BmgT120I GGNCC 2 cut(s) 924, 925
BmiI GGNNCC 1 cut(s) 926
BmsI GCATC 3 cut(s) 304, 315, 741
BmtI GCTAGC 1 cut(s) 500
BpiI GAAGAC 1 cut(s) 768
Bpu10I CCTNAGC 1 cut(s) 585
BsaAI YACGTR 1 cut(s) 379
BsaJI CCNNGG 4 cut(s) 361, 543, 907, 928
BsaXI ACNNNNNCTCC 4 cut(s) 471, 501, 837, 867
Bsc4I CCNNNNNNNGG 2 cut(s) 474, 775
BseDI CCNNGG 4 cut(s) 361, 543, 907, 928
BseGI GGATG 2 cut(s) 295, 664
BseLI CCNNNNNNNGG 2 cut(s) 474, 775
BseMII CTCAG 3 cut(s) 443, 599, 788
BseSI GKGCMC 1 cut(s) 928
BseXI GCAGC 1 cut(s) 662
Bsh1285I CGRYCG 3 cut(s) 216, 692, 885
BshFI GGCC 2 cut(s) 383, 926
BsiEI CGRYCG 3 cut(s) 216, 692, 885
BsiHKCI CYCGRG 1 cut(s) 360
BslFI GGGAC 1 cut(s) 670
BslI CCNNNNNNNGG 2 cut(s) 474, 775
BsmFI GGGAC 1 cut(s) 670
BsnI GGCC 2 cut(s) 383, 926
BsoBI CYCGRG 1 cut(s) 360
Bsp120I GGGCCC 1 cut(s) 924
Bsp1286I GDGCHC 1 cut(s) 928
Bsp1407I TGTACA 1 cut(s) 19
Bsp143I GATC 6 cut(s) 36, 150, 304, 388, 689, 882
BspACI CCGC 3 cut(s) 393, 678, 827
BspANI GGCC 2 cut(s) 383, 926
BspCNI CTCAG 3 cut(s) 442, 598, 787
BspHI TCATGA 1 cut(s) 126
BspLI GGNNCC 1 cut(s) 926
BspOI GCTAGC 1 cut(s) 500
BspPI GGATC 2 cut(s) 299, 383
BspQI GCTCTTC 1 cut(s) 191
BspTI CTTAAG 1 cut(s) 317
BsrGI TGTACA 1 cut(s) 19
BssECI CCNNGG 4 cut(s) 361, 543, 907, 928
BssMI GATC 6 cut(s) 36, 150, 304, 388, 689, 882
BssSI CACGAG 2 cut(s) 281, 741
BssT1I CCWWGG 2 cut(s) 907, 928
Bst2BI CACGAG 2 cut(s) 281, 741
Bst4CI ACNGT 1 cut(s) 607
Bst6I CTCTTC 4 cut(s) 191, 339, 543, 591
BstAFI CTTAAG 1 cut(s) 317
BstAPI GCANNNNNTGC 1 cut(s) 292
BstAUI TGTACA 1 cut(s) 19
BstBAI YACGTR 1 cut(s) 379
BstC8I GCNNGC 3 cut(s) 288, 498, 725
BstDEI CTNAG 3 cut(s) 429, 585, 774
BstDSI CCRYGG 1 cut(s) 543
BstEII GGTNACC 1 cut(s) 601
BstF5I GGATG 2 cut(s) 295, 664
BstKTI GATC 6 cut(s) 39, 153, 307, 391, 692, 885
BstMBI GATC 6 cut(s) 36, 150, 304, 388, 689, 882
BstMCI CGRYCG 3 cut(s) 216, 692, 885
BstMWI GCNNNNNNNGC 2 cut(s) 292, 729
BstNSI RCATGY 3 cut(s) 290, 593, 727
BstPI GGTNACC 1 cut(s) 601
BstSFI CTRYAG 1 cut(s) 667
BstSLI GKGCMC 1 cut(s) 928
BstV1I GCAGC 1 cut(s) 662
BstV2I GAAGAC 1 cut(s) 768
BstX2I RGATCY 1 cut(s) 36
BstYI RGATCY 1 cut(s) 36
BsuRI GGCC 2 cut(s) 383, 926
BtgI CCRYGG 1 cut(s) 543
BtsCI GGATG 2 cut(s) 295, 664
BtsI GCAGTG 1 cut(s) 268
BtsIMutI CAGTG 2 cut(s) 268, 862
Cac8I GCNNGC 3 cut(s) 288, 498, 725
CciI TCATGA 1 cut(s) 126
Cfr13I GGNCC 2 cut(s) 924, 925
CseI GACGC 1 cut(s) 746
Csp6I GTAC 3 cut(s) 20, 408, 450
CviAII CATG 9 cut(s) 127, 148, 287, 419, 424, 590, 724, 757, 815
CviQI GTAC 3 cut(s) 20, 408, 450
DdeI CTNAG 3 cut(s) 429, 585, 774
DpnI GATC 6 cut(s) 38, 152, 306, 390, 691, 884
DpnII GATC 6 cut(s) 36, 150, 304, 388, 689, 882
EaeI YGGCCR 1 cut(s) 381
Eam1104I CTCTTC 4 cut(s) 191, 339, 543, 591
EarI CTCTTC 4 cut(s) 191, 339, 543, 591
Eco130I CCWWGG 2 cut(s) 907, 928
Eco24I GRGCYC 1 cut(s) 928
Eco57I CTGAAG 1 cut(s) 65
Eco88I CYCGRG 1 cut(s) 360
Eco91I GGTNACC 1 cut(s) 601
EcoO109I RGGNCCY 1 cut(s) 925
EcoO65I GGTNACC 1 cut(s) 601
EcoT14I CCWWGG 2 cut(s) 907, 928
EcoT38I GRGCYC 1 cut(s) 928
ErhI CCWWGG 2 cut(s) 907, 928
FaeI CATG 9 cut(s) 130, 151, 290, 422, 427, 593, 727, 760, 818
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FaqI GGGAC 1 cut(s) 670
FatI CATG 9 cut(s) 126, 147, 286, 418, 423, 589, 723, 756, 814
FauI CCCGC 2 cut(s) 400, 671
FbaI TGATCA 1 cut(s) 150
Fnu4HI GCNGC 2 cut(s) 676, 828
FokI GGATG 2 cut(s) 282, 671
FriOI GRGCYC 1 cut(s) 928
Fsp4HI GCNGC 2 cut(s) 676, 828
FspBI CTAG 3 cut(s) 497, 897, 908
GluI GCNGC 2 cut(s) 676, 828
HaeIII GGCC 2 cut(s) 383, 926
HgaI GACGC 1 cut(s) 746
Hin1II CATG 9 cut(s) 130, 151, 290, 422, 427, 593, 727, 760, 818
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 2 cut(s) 123, 427
HphI GGTGA 4 cut(s) 524, 547, 806, 860
Hpy166II GTNNAC 4 cut(s) 6, 109, 452, 610
Hpy188I TCNGA 1 cut(s) 821
Hpy188III TCNNGA 7 cut(s) 43, 127, 193, 247, 687, 743, 889
Hpy8I GTNNAC 4 cut(s) 6, 109, 452, 610
Hpy99I CGWCG 2 cut(s) 389, 740
HpyAV CCTTC 3 cut(s) 229, 467, 695
HpyCH4III ACNGT 1 cut(s) 607
HpyCH4IV ACGT 2 cut(s) 378, 612
HpyCH4V TGCA 4 cut(s) 86, 273, 295, 437
HpyF10VI GCNNNNNNNGC 2 cut(s) 292, 729
HpyF3I CTNAG 3 cut(s) 429, 585, 774
HpySE526I ACGT 2 cut(s) 378, 612
Hsp92II CATG 9 cut(s) 130, 151, 290, 422, 427, 593, 727, 760, 818
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 6 cut(s) 36, 150, 304, 388, 689, 882
LguI GCTCTTC 1 cut(s) 191
LmnI GCTCC 1 cut(s) 575
Lsp1109I GCAGC 1 cut(s) 662
LweI GCATC 3 cut(s) 304, 315, 741
MaeI CTAG 3 cut(s) 497, 897, 908
MaeII ACGT 2 cut(s) 378, 612
MaeIII GTNAC 3 cut(s) 413, 601, 743
MalI GATC 6 cut(s) 38, 152, 306, 390, 691, 884
MboI GATC 6 cut(s) 36, 150, 304, 388, 689, 882
MboII GAAGA 7 cut(s) 31, 208, 334, 356, 530, 608, 773
MfeI CAATTG 1 cut(s) 54
MflI RGATCY 1 cut(s) 36
MhlI GDGCHC 1 cut(s) 928
MluCI AATT 2 cut(s) 54, 567
MlyI GAGTC 1 cut(s) 421
MmeI TCCRAC 1 cut(s) 799
MnlI CCTC 9 cut(s) 151, 319, 356, 473, 546, 592, 594, 839, 844
MseI TTAA 2 cut(s) 159, 318
MslI CAYNNNNRTG 2 cut(s) 285, 755
MspCI CTTAAG 1 cut(s) 317
MunI CAATTG 1 cut(s) 54
MwoI GCNNNNNNNGC 2 cut(s) 292, 729
NdeII GATC 6 cut(s) 36, 150, 304, 388, 689, 882
NheI GCTAGC 1 cut(s) 496
NlaIII CATG 9 cut(s) 130, 151, 290, 422, 427, 593, 727, 760, 818
NlaIV GGNNCC 1 cut(s) 926
NmuCI GTSAC 1 cut(s) 743
NspI RCATGY 3 cut(s) 290, 593, 727
PaeI GCATGC 2 cut(s) 290, 727
PagI TCATGA 1 cut(s) 126
PciSI GCTCTTC 1 cut(s) 191
PcsI WCGNNNNNNNCGW 1 cut(s) 384
PfeI GAWTC 1 cut(s) 123
PkrI GCNGC 2 cut(s) 677, 829
Ple19I CGATCG 2 cut(s) 692, 885
PleI GAGTC 1 cut(s) 421
PpsI GAGTC 1 cut(s) 421
Ppu21I YACGTR 1 cut(s) 379
Psp1406I AACGTT 1 cut(s) 612
PspEI GGTNACC 1 cut(s) 601
PspN4I GGNNCC 1 cut(s) 926
PspOMI GGGCCC 1 cut(s) 924
PspPI GGNCC 2 cut(s) 924, 925
PsuI RGATCY 1 cut(s) 36
PvuI CGATCG 2 cut(s) 692, 885
RsaI GTAC 3 cut(s) 21, 409, 451
RsaNI GTAC 3 cut(s) 20, 408, 450
RseI CAYNNNNRTG 2 cut(s) 285, 755
SapI GCTCTTC 1 cut(s) 191
SaqAI TTAA 2 cut(s) 159, 318
SatI GCNGC 2 cut(s) 676, 828
Sau3AI GATC 6 cut(s) 36, 150, 304, 388, 689, 882
Sau96I GGNCC 2 cut(s) 924, 925
SchI GAGTC 1 cut(s) 421
SduI GDGCHC 1 cut(s) 928
SfaNI GCATC 3 cut(s) 304, 315, 741
SfcI CTRYAG 1 cut(s) 667
SmiMI CAYNNNNRTG 2 cut(s) 285, 755
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
SphI GCATGC 2 cut(s) 290, 727
Sse9I AATT 2 cut(s) 54, 567
SsiI CCGC 3 cut(s) 393, 678, 827
SspI AATATT 1 cut(s) 529
SspMI CTAG 3 cut(s) 497, 897, 908
StyI CCWWGG 2 cut(s) 907, 928
TaaI ACNGT 1 cut(s) 607
TaiI ACGT 2 cut(s) 381, 615
TaqI TCGA 2 cut(s) 490, 735
TaqII GACCGA 3 cut(s) 75, 230, 682
TasI AATT 2 cut(s) 54, 567
TatI WGTACW 1 cut(s) 19
TauI GCSGC 1 cut(s) 830
TfiI GAWTC 1 cut(s) 123
Tru1I TTAA 2 cut(s) 159, 318
Tru9I TTAA 2 cut(s) 159, 318
TscAI CASTG 2 cut(s) 275, 862
TseFI GTSAC 1 cut(s) 743
TseI GCWGC 1 cut(s) 675
Tsp45I GTSAC 1 cut(s) 743
TspDTI ATGAA 3 cut(s) 115, 248, 486
TspRI CASTG 2 cut(s) 275, 862
Vha464I CTTAAG 1 cut(s) 317
XceI RCATGY 3 cut(s) 290, 593, 727
XmaJI CCTAGG 1 cut(s) 907
XspI CTAG 3 cut(s) 497, 897, 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.