Rh1DG325000

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0012696
Physical Location & Seq
Forward (+)
55078153 .. 55078812
660 bp
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UTR
Exon/CDS
Intron
Rh1DG325000.1

Sequence Viewer

Length: 414 bp
ATGTCCACATTCATAGATGCACAAAGAGAAACCGACATCTTCTTGAAGCTTACAATTGACCGAGAGAACAGGAAGGTATTGTTTGCAGAAGCAAACAAGGACTTTGTTGACAATCTGGTGGGATTCATGACTTTTCCTACCTCTGCCATGATTAAGCTTAGCCCTGAGCCTTTTGGCAAGCTAAAAAATGTTAAAAATAGCATTGAGAACATGACCGAGTATTACAATCCGATGAAGGCTTCTCTCCTGAATACAATGCCACCGGCTCACCGCAATCCCTGTGTCCCCTTGATCCCTCATCACTTGAGAATATGCCCTTTTATGTATGTGAAGAGTGTATTACTACCCGAGGTAAGCCCTATTATGTGGCCAACGATCCCACAGTCTTGTGCCCTACTTGTAACCATGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

137

Amino Acids

15.64

Weight (kDa)

8.48

Isoelectric Point (pI)

48.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 10 - 73 7.5e-11 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 271
AclWI GGATC 2 cut(s) 286, 370
AcoI YGGCCR 1 cut(s) 368
AgsI TTSAA 1 cut(s) 46
AluBI AGCT 3 cut(s) 49, 157, 181
AluI AGCT 3 cut(s) 49, 157, 181
AlwI GGATC 2 cut(s) 286, 370
Ama87I CYCGRG 1 cut(s) 347
AoxI GGCC 1 cut(s) 368
AsuHPI GGTGA 1 cut(s) 260
AvaI CYCGRG 1 cut(s) 347
BaeGI GKGCMC 1 cut(s) 394
BalI TGGCCA 1 cut(s) 370
BlpI GCTNAGC 1 cut(s) 158
BmeT110I CYCGRG 1 cut(s) 347
BmsI GCATC 1 cut(s) 7
Bpu10I CCTNAGC 1 cut(s) 165
Bpu1102I GCTNAGC 1 cut(s) 158
BpuEI CTTGAG 1 cut(s) 325
BsaJI CCNNGG 1 cut(s) 348
Bse118I RCCGGY 1 cut(s) 262
BseDI CCNNGG 1 cut(s) 348
BseMII CTCAG 1 cut(s) 156
BseSI GKGCMC 1 cut(s) 394
BshFI GGCC 1 cut(s) 370
BsiHKCI CYCGRG 1 cut(s) 347
BsiSI CCGG 1 cut(s) 263
BslFI GGGAC 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 269
BsnI GGCC 1 cut(s) 370
BsoBI CYCGRG 1 cut(s) 347
Bsp1286I GDGCHC 1 cut(s) 394
Bsp143I GATC 2 cut(s) 291, 375
Bsp1720I GCTNAGC 1 cut(s) 158
BspACI CCGC 1 cut(s) 271
BspANI GGCC 1 cut(s) 370
BspCNI CTCAG 1 cut(s) 157
BspHI TCATGA 1 cut(s) 126
BspPI GGATC 2 cut(s) 286, 370
BsrFI RCCGGY 1 cut(s) 262
BssAI RCCGGY 1 cut(s) 262
BssECI CCNNGG 1 cut(s) 348
BssMI GATC 2 cut(s) 291, 375
Bst4CI ACNGT 1 cut(s) 384
Bst6I CTCTTC 1 cut(s) 326
BstC8I GCNNGC 1 cut(s) 179
BstDEI CTNAG 2 cut(s) 158, 165
BstKTI GATC 2 cut(s) 294, 378
BstMBI GATC 2 cut(s) 291, 375
BstSLI GKGCMC 1 cut(s) 394
BsuRI GGCC 1 cut(s) 370
Cac8I GCNNGC 1 cut(s) 179
CciI TCATGA 1 cut(s) 126
Cfr10I RCCGGY 1 cut(s) 262
CviAII CATG 5 cut(s) 127, 148, 211, 406, 411
CviJI RGCY 9 cut(s) 49, 157, 162, 169, 181, 239, 266, 357, 370
CviKI_1 RGCY 9 cut(s) 49, 157, 162, 169, 181, 239, 266, 357, 370
DdeI CTNAG 2 cut(s) 158, 165
DpnI GATC 2 cut(s) 293, 377
DpnII GATC 2 cut(s) 291, 375
EaeI YGGCCR 1 cut(s) 368
Eam1104I CTCTTC 1 cut(s) 326
EarI CTCTTC 1 cut(s) 326
Eco88I CYCGRG 1 cut(s) 347
FaeI CATG 5 cut(s) 130, 151, 214, 409, 414
FaqI GGGAC 1 cut(s) 269
FatI CATG 5 cut(s) 126, 147, 210, 405, 410
HaeIII GGCC 1 cut(s) 370
HapII CCGG 1 cut(s) 263
Hin1II CATG 5 cut(s) 130, 151, 214, 409, 414
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HindIII AAGCTT 2 cut(s) 47, 155
HinfI GANTC 1 cut(s) 123
HpaII CCGG 1 cut(s) 263
HphI GGTGA 1 cut(s) 260
Hpy166II GTNNAC 2 cut(s) 6, 109
Hpy188I TCNGA 1 cut(s) 231
Hpy188III TCNNGA 3 cut(s) 43, 127, 247
Hpy8I GTNNAC 2 cut(s) 6, 109
HpyAV CCTTC 2 cut(s) 67, 229
HpyCH4III ACNGT 1 cut(s) 384
HpyCH4V TGCA 2 cut(s) 20, 86
HpyF3I CTNAG 2 cut(s) 158, 165
Hsp92II CATG 5 cut(s) 130, 151, 214, 409, 414
Kzo9I GATC 2 cut(s) 291, 375
LpnPI CCDG 6 cut(s) 55, 101, 177, 260, 276, 292
LweI GCATC 1 cut(s) 7
MaeIII GTNAC 1 cut(s) 400
MalI GATC 2 cut(s) 293, 377
MboI GATC 2 cut(s) 291, 375
MboII GAAGA 2 cut(s) 31, 343
MfeI CAATTG 1 cut(s) 54
MhlI GDGCHC 1 cut(s) 394
MlsI TGGCCA 1 cut(s) 370
MluCI AATT 1 cut(s) 54
MluNI TGGCCA 1 cut(s) 370
MnlI CCTC 3 cut(s) 151, 306, 343
Mox20I TGGCCA 1 cut(s) 370
MscI TGGCCA 1 cut(s) 370
MseI TTAA 2 cut(s) 153, 192
Msp20I TGGCCA 1 cut(s) 370
MspI CCGG 1 cut(s) 263
MunI CAATTG 1 cut(s) 54
NdeII GATC 2 cut(s) 291, 375
NlaIII CATG 5 cut(s) 130, 151, 214, 409, 414
PagI TCATGA 1 cut(s) 126
PfeI GAWTC 1 cut(s) 123
SaqAI TTAA 2 cut(s) 153, 192
Sau3AI GATC 2 cut(s) 291, 375
SduI GDGCHC 1 cut(s) 394
SetI ASST 6 cut(s) 51, 78, 143, 159, 183, 354
SfaNI GCATC 1 cut(s) 7
SmlI CTYRAG 1 cut(s) 304
SmoI CTYRAG 1 cut(s) 304
Sse9I AATT 1 cut(s) 54
SsiI CCGC 1 cut(s) 271
TaaI ACNGT 1 cut(s) 384
TaqII GACCGA 2 cut(s) 75, 230
TasI AATT 1 cut(s) 54
TfiI GAWTC 1 cut(s) 123
Tru1I TTAA 2 cut(s) 153, 192
Tru9I TTAA 2 cut(s) 153, 192
TspDTI ATGAA 2 cut(s) 115, 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.