Rroxscaffold_4G00291050

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0012696
Physical Location & Seq
Reverse (-)
11470018 .. 11471456
1439 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00291050.1

Sequence Viewer

Length: 717 bp
ATGTCCACATTCATAGATATACAAAGAGAAACCGACATCTTCTTGAAGCTTACAATTGACCGAGAGAACAGGAAGGTATTGTTTGCAGAAGCAAACAAGGACTTTGTTGACAATCTGGTGGGATTCATGACTTTTCCTACCTCTGCCATGATTAAGCTTAGCCCTGAGCCTTTTGGCAAGTTAAACAATGTTAAAAATAGCATTGAGAACATGACCGAGCATTACAATCCGATGAAGGCTTCTCTCCTGAATACAATGCCACCGGCTCACCGCAATCGTCACGAGCATGCCCTGTGTCCCCTTGATCCCTCATCACTTGAGAATATGCCCTTTTATGCATGTGAAGAGTGTATTACTACCAGAGGTAAGCCCTATTATGTGGCCAACGATCCCACAGCCTTATGCCCTACTTGTAACCATGCCATGACTCAAGGTGCAAGTTATTGCCGTACACCTTCTGAAACAGCCAATGAAGGGGGAGGTTTTGTTCTACAGCGAGTGAAATACATTATTACTGATGATTTGAATGTTCACCCTTTTCTCACGGCTTCTCTCATCAGAAGGATAATTCTTGGAGCTAATTCGCAGCATGTTGAAGAGGTTACCGTGAACGTTTCCCATAAGAAGCTGGTGATGCTGGAGTTGTTAAAAAAGGCTATGCAAAACTCCAACACGGTGTTGTCAGATGTATTTTTCTGGGATGGATGGATTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

238

Amino Acids

26.9

Weight (kDa)

6.24

Isoelectric Point (pI)

43.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 10 - 232 5.1e-25 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 271
AclI AACGTT 1 cut(s) 612
AclWI GGATC 2 cut(s) 299, 383
AcoI YGGCCR 1 cut(s) 381
AfaI GTAC 1 cut(s) 451
AfiI CCNNNNNNNGG 1 cut(s) 474
AgsI TTSAA 3 cut(s) 46, 526, 596
AloI GAACNNNNNNTCC 2 cut(s) 471, 503
AluBI AGCT 4 cut(s) 49, 157, 578, 628
AluI AGCT 4 cut(s) 49, 157, 578, 628
AlwI GGATC 2 cut(s) 299, 383
AoxI GGCC 1 cut(s) 381
ApeKI GCWGC 1 cut(s) 586
AsuHPI GGTGA 3 cut(s) 260, 524, 643
BalI TGGCCA 1 cut(s) 383
BauI CACGAG 1 cut(s) 281
BbvI GCAGC 1 cut(s) 598
BccI CCATC 2 cut(s) 695, 699
BceAI ACGGC 2 cut(s) 432, 561
BfmI CTRYAG 1 cut(s) 491
BisI GCNGC 1 cut(s) 587
BlpI GCTNAGC 1 cut(s) 158
BlsI GCNGC 1 cut(s) 588
BmsI GCATC 1 cut(s) 624
BpmI CTGGAG 1 cut(s) 659
Bpu10I CCTNAGC 1 cut(s) 165
Bpu1102I GCTNAGC 1 cut(s) 158
BpuEI CTTGAG 2 cut(s) 338, 414
BsaXI ACNNNNNCTCC 2 cut(s) 471, 501
Bsc4I CCNNNNNNNGG 1 cut(s) 474
Bse118I RCCGGY 1 cut(s) 262
BseGI GGATG 2 cut(s) 706, 710
BseLI CCNNNNNNNGG 1 cut(s) 474
BseMII CTCAG 1 cut(s) 156
BseXI GCAGC 1 cut(s) 598
BshFI GGCC 1 cut(s) 383
BsiSI CCGG 1 cut(s) 263
BslFI GGGAC 1 cut(s) 282
BslI CCNNNNNNNGG 1 cut(s) 474
BsmFI GGGAC 1 cut(s) 282
BsnI GGCC 1 cut(s) 383
Bsp143I GATC 2 cut(s) 304, 388
Bsp1720I GCTNAGC 1 cut(s) 158
BspACI CCGC 1 cut(s) 271
BspANI GGCC 1 cut(s) 383
BspCNI CTCAG 1 cut(s) 157
BspHI TCATGA 1 cut(s) 126
BspPI GGATC 2 cut(s) 299, 383
BsrFI RCCGGY 1 cut(s) 262
BssAI RCCGGY 1 cut(s) 262
BssMI GATC 2 cut(s) 304, 388
BssSI CACGAG 1 cut(s) 281
Bst2BI CACGAG 1 cut(s) 281
Bst4CI ACNGT 2 cut(s) 607, 676
Bst6I CTCTTC 2 cut(s) 339, 591
BstC8I GCNNGC 1 cut(s) 288
BstDEI CTNAG 2 cut(s) 158, 165
BstEII GGTNACC 1 cut(s) 601
BstF5I GGATG 2 cut(s) 706, 710
BstKTI GATC 2 cut(s) 307, 391
BstMBI GATC 2 cut(s) 304, 388
BstMWI GCNNNNNNNGC 1 cut(s) 634
BstNSI RCATGY 3 cut(s) 290, 342, 593
BstPI GGTNACC 1 cut(s) 601
BstSFI CTRYAG 1 cut(s) 491
BstV1I GCAGC 1 cut(s) 598
BsuRI GGCC 1 cut(s) 383
BtsCI GGATG 2 cut(s) 706, 710
Cac8I GCNNGC 1 cut(s) 288
CciI TCATGA 1 cut(s) 126
Cfr10I RCCGGY 1 cut(s) 262
Csp6I GTAC 1 cut(s) 450
CviAII CATG 8 cut(s) 127, 148, 211, 287, 339, 419, 424, 590
CviQI GTAC 1 cut(s) 450
DdeI CTNAG 2 cut(s) 158, 165
DpnI GATC 2 cut(s) 306, 390
DpnII GATC 2 cut(s) 304, 388
EaeI YGGCCR 1 cut(s) 381
Eam1104I CTCTTC 2 cut(s) 339, 591
EarI CTCTTC 2 cut(s) 339, 591
Eco91I GGTNACC 1 cut(s) 601
EcoO65I GGTNACC 1 cut(s) 601
EcoT22I ATGCAT 1 cut(s) 340
FaeI CATG 8 cut(s) 130, 151, 214, 290, 342, 422, 427, 593
FaqI GGGAC 1 cut(s) 282
FatI CATG 8 cut(s) 126, 147, 210, 286, 338, 418, 423, 589
Fnu4HI GCNGC 1 cut(s) 587
FokI GGATG 1 cut(s) 713
Fsp4HI GCNGC 1 cut(s) 587
GluI GCNGC 1 cut(s) 587
GsuI CTGGAG 1 cut(s) 659
HaeIII GGCC 1 cut(s) 383
HapII CCGG 1 cut(s) 263
Hin1II CATG 8 cut(s) 130, 151, 214, 290, 342, 422, 427, 593
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HindIII AAGCTT 2 cut(s) 47, 155
HinfI GANTC 2 cut(s) 123, 427
HpaII CCGG 1 cut(s) 263
HphI GGTGA 3 cut(s) 260, 524, 643
Hpy166II GTNNAC 5 cut(s) 6, 109, 452, 532, 610
Hpy188I TCNGA 4 cut(s) 231, 460, 560, 685
Hpy188III TCNNGA 4 cut(s) 43, 127, 247, 281
Hpy8I GTNNAC 5 cut(s) 6, 109, 452, 532, 610
HpyAV CCTTC 5 cut(s) 67, 229, 465, 467, 555
HpyCH4III ACNGT 2 cut(s) 607, 676
HpyCH4IV ACGT 1 cut(s) 612
HpyCH4V TGCA 4 cut(s) 86, 338, 437, 661
HpyF10VI GCNNNNNNNGC 1 cut(s) 634
HpyF3I CTNAG 2 cut(s) 158, 165
HpySE526I ACGT 1 cut(s) 612
Hsp92II CATG 8 cut(s) 130, 151, 214, 290, 342, 422, 427, 593
Kzo9I GATC 2 cut(s) 304, 388
LmnI GCTCC 1 cut(s) 575
Lsp1109I GCAGC 1 cut(s) 598
LweI GCATC 1 cut(s) 624
MaeII ACGT 1 cut(s) 612
MaeIII GTNAC 3 cut(s) 278, 413, 601
MalI GATC 2 cut(s) 306, 390
MboI GATC 2 cut(s) 304, 388
MboII GAAGA 3 cut(s) 31, 356, 608
MfeI CAATTG 1 cut(s) 54
MlsI TGGCCA 1 cut(s) 383
MluCI AATT 3 cut(s) 54, 567, 580
MluNI TGGCCA 1 cut(s) 383
MlyI GAGTC 1 cut(s) 421
MmeI TCCRAC 1 cut(s) 693
MnlI CCTC 5 cut(s) 151, 319, 356, 473, 592
Mox20I TGGCCA 1 cut(s) 383
Mph1103I ATGCAT 1 cut(s) 340
MscI TGGCCA 1 cut(s) 383
MseI TTAA 4 cut(s) 153, 182, 192, 647
MslI CAYNNNNRTG 1 cut(s) 285
Msp20I TGGCCA 1 cut(s) 383
MspI CCGG 1 cut(s) 263
MunI CAATTG 1 cut(s) 54
MwoI GCNNNNNNNGC 1 cut(s) 634
NdeII GATC 2 cut(s) 304, 388
NlaIII CATG 8 cut(s) 130, 151, 214, 290, 342, 422, 427, 593
NmuCI GTSAC 1 cut(s) 278
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 3 cut(s) 290, 342, 593
PaeI GCATGC 1 cut(s) 290
PagI TCATGA 1 cut(s) 126
PfeI GAWTC 1 cut(s) 123
PkrI GCNGC 1 cut(s) 588
PleI GAGTC 1 cut(s) 421
PpsI GAGTC 1 cut(s) 421
Psp1406I AACGTT 1 cut(s) 612
PspEI GGTNACC 1 cut(s) 601
RsaI GTAC 1 cut(s) 451
RsaNI GTAC 1 cut(s) 450
RseI CAYNNNNRTG 1 cut(s) 285
SaqAI TTAA 4 cut(s) 153, 182, 192, 647
SatI GCNGC 1 cut(s) 587
Sau3AI GATC 2 cut(s) 304, 388
SchI GAGTC 1 cut(s) 421
SfaNI GCATC 1 cut(s) 624
SfcI CTRYAG 1 cut(s) 491
SmiMI CAYNNNNRTG 1 cut(s) 285
SmlI CTYRAG 2 cut(s) 317, 429
SmoI CTYRAG 2 cut(s) 317, 429
SphI GCATGC 1 cut(s) 290
Sse9I AATT 3 cut(s) 54, 567, 580
SsiI CCGC 1 cut(s) 271
TaaI ACNGT 2 cut(s) 607, 676
TaiI ACGT 1 cut(s) 615
TaqII GACCGA 2 cut(s) 75, 230
TasI AATT 3 cut(s) 54, 567, 580
TfiI GAWTC 1 cut(s) 123
Tru1I TTAA 4 cut(s) 153, 182, 192, 647
Tru9I TTAA 4 cut(s) 153, 182, 192, 647
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 1 cut(s) 586
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 3 cut(s) 115, 248, 486
XceI RCATGY 3 cut(s) 290, 342, 593
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.