Rh6DG030700

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0012696
Physical Location & Seq
Reverse (-)
3018768 .. 3019580
813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG030700.1

Sequence Viewer

Length: 717 bp
ATGTCCACATTCATAAATGTACAAAGAGAAACCGAGATCTTCCTGAAGCTTACAATTGACCGAGAGAACAGAAAGGTATTGTTTGCAGAAGCAAACAAGGATTTTGTTGACAATCTGGTGGGATTCATGATTTTCCCTACCTCTGCCATGATCACGCTTAACCCTGAACCTTTTGGAAAGCTAAACAATGTCAAGAAGAGCATTGAGAACATGACCGAGTATTACAATCCAATGAAGGCTTCTCTCCTGAATACAAAGCCACCAGCTCACTGCAATCGGCACGAGCATGCCCTGCATCCCCTTGATCCCTCATCACTTAAGAAGATGCCCTTTTATGTATGTGAAGAGTGTATTACTACCCGAGGTAAGCCATACTATGTGGCCGACGATCCCGCAGTCTTGTGTCCTACTTGTAACCATGCCATGACTCAGGGTGCAAGTTATTGTGGTACACCTATTGAAATCGCCAATGAAGGGGGAGGTTTTGTTCGACAGCTAGCGAAATACATCATTACTGATGATTTGAATATTCACCCTCTTCTCACGGTTTCTCTCACCACAAACATAATTCGTGGAGCTAAACCTCAGCATGTTGAAGAGGTTACCGTGAACGTTTCTCATAAGAAGTTGGTGATGCTGGAGTTGTTAAAAAAGGCTATGCAAAACTGCGACACGGTGTTGTCGGATGTATTTTTCGCTGATGGATGGATTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

238

Amino Acids

26.75

Weight (kDa)

6.44

Isoelectric Point (pI)

36.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 10 - 232 5.6e-20 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 393
AclI AACGTT 1 cut(s) 612
AclWI GGATC 2 cut(s) 299, 383
AcoI YGGCCR 1 cut(s) 381
AcuI CTGAAG 1 cut(s) 65
AfaI GTAC 2 cut(s) 21, 451
AfiI CCNNNNNNNGG 1 cut(s) 474
AflII CTTAAG 1 cut(s) 317
AgsI TTSAA 3 cut(s) 461, 526, 596
AjuI GAANNNNNNNTTGG 2 cut(s) 223, 255
AloI GAACNNNNNNTCC 2 cut(s) 471, 503
AluBI AGCT 5 cut(s) 49, 181, 266, 496, 578
AluI AGCT 5 cut(s) 49, 181, 266, 496, 578
AlwI GGATC 2 cut(s) 299, 383
Ama87I CYCGRG 1 cut(s) 360
AoxI GGCC 1 cut(s) 381
AsuHPI GGTGA 3 cut(s) 524, 547, 643
AsuNHI GCTAGC 1 cut(s) 496
AvaI CYCGRG 1 cut(s) 360
BauI CACGAG 1 cut(s) 281
BbvCI CCTCAGC 1 cut(s) 585
BccI CCATC 2 cut(s) 695, 699
BclI TGATCA 1 cut(s) 150
BfaI CTAG 1 cut(s) 497
BfrI CTTAAG 1 cut(s) 317
BglII AGATCT 1 cut(s) 36
BmeT110I CYCGRG 1 cut(s) 360
BmsI GCATC 3 cut(s) 304, 315, 624
BmtI GCTAGC 1 cut(s) 500
BpmI CTGGAG 1 cut(s) 659
Bpu10I CCTNAGC 1 cut(s) 585
BsaJI CCNNGG 1 cut(s) 361
BsaXI ACNNNNNCTCC 2 cut(s) 471, 501
Bsc4I CCNNNNNNNGG 1 cut(s) 474
BseDI CCNNGG 1 cut(s) 361
BseGI GGATG 3 cut(s) 295, 691, 710
BseLI CCNNNNNNNGG 1 cut(s) 474
BseMII CTCAG 2 cut(s) 443, 599
BshFI GGCC 1 cut(s) 383
BsiHKCI CYCGRG 1 cut(s) 360
BslI CCNNNNNNNGG 1 cut(s) 474
BsnI GGCC 1 cut(s) 383
BsoBI CYCGRG 1 cut(s) 360
Bsp1407I TGTACA 1 cut(s) 19
Bsp143I GATC 4 cut(s) 36, 150, 304, 388
BspACI CCGC 1 cut(s) 393
BspANI GGCC 1 cut(s) 383
BspCNI CTCAG 2 cut(s) 442, 598
BspHI TCATGA 1 cut(s) 126
BspOI GCTAGC 1 cut(s) 500
BspPI GGATC 2 cut(s) 299, 383
BspQI GCTCTTC 1 cut(s) 191
BspTI CTTAAG 1 cut(s) 317
BsrGI TGTACA 1 cut(s) 19
BssECI CCNNGG 1 cut(s) 361
BssMI GATC 4 cut(s) 36, 150, 304, 388
BssSI CACGAG 1 cut(s) 281
Bst2BI CACGAG 1 cut(s) 281
Bst4CI ACNGT 3 cut(s) 547, 607, 676
Bst6I CTCTTC 4 cut(s) 191, 339, 543, 591
BstAFI CTTAAG 1 cut(s) 317
BstAPI GCANNNNNTGC 1 cut(s) 292
BstAUI TGTACA 1 cut(s) 19
BstC8I GCNNGC 2 cut(s) 288, 498
BstDEI CTNAG 2 cut(s) 429, 585
BstEII GGTNACC 1 cut(s) 601
BstF5I GGATG 3 cut(s) 295, 691, 710
BstKTI GATC 4 cut(s) 39, 153, 307, 391
BstMBI GATC 4 cut(s) 36, 150, 304, 388
BstMWI GCNNNNNNNGC 1 cut(s) 292
BstNSI RCATGY 2 cut(s) 290, 593
BstPI GGTNACC 1 cut(s) 601
BstX2I RGATCY 1 cut(s) 36
BstYI RGATCY 1 cut(s) 36
BsuRI GGCC 1 cut(s) 383
BtsCI GGATG 3 cut(s) 295, 691, 710
BtsI GCAGTG 1 cut(s) 268
BtsIMutI CAGTG 1 cut(s) 268
Cac8I GCNNGC 2 cut(s) 288, 498
CciI TCATGA 1 cut(s) 126
Csp6I GTAC 2 cut(s) 20, 450
CviAII CATG 7 cut(s) 127, 148, 211, 287, 419, 424, 590
CviQI GTAC 2 cut(s) 20, 450
DdeI CTNAG 2 cut(s) 429, 585
DpnI GATC 4 cut(s) 38, 152, 306, 390
DpnII GATC 4 cut(s) 36, 150, 304, 388
EaeI YGGCCR 1 cut(s) 381
Eam1104I CTCTTC 4 cut(s) 191, 339, 543, 591
EarI CTCTTC 4 cut(s) 191, 339, 543, 591
Eco57I CTGAAG 1 cut(s) 65
Eco88I CYCGRG 1 cut(s) 360
Eco91I GGTNACC 1 cut(s) 601
EcoO65I GGTNACC 1 cut(s) 601
FaeI CATG 7 cut(s) 130, 151, 214, 290, 422, 427, 593
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FatI CATG 7 cut(s) 126, 147, 210, 286, 418, 423, 589
FauI CCCGC 1 cut(s) 400
FbaI TGATCA 1 cut(s) 150
FokI GGATG 2 cut(s) 282, 698
FspBI CTAG 1 cut(s) 497
GsuI CTGGAG 1 cut(s) 659
HaeIII GGCC 1 cut(s) 383
Hin1II CATG 7 cut(s) 130, 151, 214, 290, 422, 427, 593
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 2 cut(s) 123, 427
HphI GGTGA 3 cut(s) 524, 547, 643
Hpy166II GTNNAC 4 cut(s) 6, 109, 452, 610
Hpy188I TCNGA 1 cut(s) 685
Hpy188III TCNNGA 4 cut(s) 43, 127, 193, 247
Hpy8I GTNNAC 4 cut(s) 6, 109, 452, 610
Hpy99I CGWCG 1 cut(s) 389
HpyAV CCTTC 2 cut(s) 229, 467
HpyCH4III ACNGT 3 cut(s) 547, 607, 676
HpyCH4IV ACGT 1 cut(s) 612
HpyCH4V TGCA 5 cut(s) 86, 273, 295, 437, 661
HpyF10VI GCNNNNNNNGC 1 cut(s) 292
HpyF3I CTNAG 2 cut(s) 429, 585
HpySE526I ACGT 1 cut(s) 612
Hsp92II CATG 7 cut(s) 130, 151, 214, 290, 422, 427, 593
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 4 cut(s) 36, 150, 304, 388
LguI GCTCTTC 1 cut(s) 191
LmnI GCTCC 1 cut(s) 575
LpnPI CCDG 8 cut(s) 56, 101, 177, 260, 276, 305, 416, 623
LweI GCATC 3 cut(s) 304, 315, 624
MaeI CTAG 1 cut(s) 497
MaeII ACGT 1 cut(s) 612
MaeIII GTNAC 2 cut(s) 413, 601
MalI GATC 4 cut(s) 38, 152, 306, 390
MboI GATC 4 cut(s) 36, 150, 304, 388
MboII GAAGA 6 cut(s) 31, 208, 334, 356, 530, 608
MfeI CAATTG 1 cut(s) 54
MflI RGATCY 1 cut(s) 36
MluCI AATT 2 cut(s) 54, 567
MlyI GAGTC 1 cut(s) 421
MmeI TCCRAC 1 cut(s) 663
MnlI CCTC 7 cut(s) 151, 319, 356, 473, 546, 592, 594
MseI TTAA 3 cut(s) 159, 318, 647
MslI CAYNNNNRTG 1 cut(s) 285
MspCI CTTAAG 1 cut(s) 317
MunI CAATTG 1 cut(s) 54
MwoI GCNNNNNNNGC 1 cut(s) 292
NdeII GATC 4 cut(s) 36, 150, 304, 388
NheI GCTAGC 1 cut(s) 496
NlaIII CATG 7 cut(s) 130, 151, 214, 290, 422, 427, 593
NspI RCATGY 2 cut(s) 290, 593
PaeI GCATGC 1 cut(s) 290
PagI TCATGA 1 cut(s) 126
PciSI GCTCTTC 1 cut(s) 191
PfeI GAWTC 1 cut(s) 123
PleI GAGTC 1 cut(s) 421
PpsI GAGTC 1 cut(s) 421
Psp1406I AACGTT 1 cut(s) 612
PspEI GGTNACC 1 cut(s) 601
PsuI RGATCY 1 cut(s) 36
RsaI GTAC 2 cut(s) 21, 451
RsaNI GTAC 2 cut(s) 20, 450
RseI CAYNNNNRTG 1 cut(s) 285
SapI GCTCTTC 1 cut(s) 191
SaqAI TTAA 3 cut(s) 159, 318, 647
Sau3AI GATC 4 cut(s) 36, 150, 304, 388
SchI GAGTC 1 cut(s) 421
SfaNI GCATC 3 cut(s) 304, 315, 624
SmiMI CAYNNNNRTG 1 cut(s) 285
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
SphI GCATGC 1 cut(s) 290
Sse9I AATT 2 cut(s) 54, 567
SsiI CCGC 1 cut(s) 393
SspI AATATT 1 cut(s) 529
SspMI CTAG 1 cut(s) 497
TaaI ACNGT 3 cut(s) 547, 607, 676
TaiI ACGT 1 cut(s) 615
TaqI TCGA 1 cut(s) 490
TaqII GACCGA 2 cut(s) 75, 230
TasI AATT 2 cut(s) 54, 567
TatI WGTACW 1 cut(s) 19
TfiI GAWTC 1 cut(s) 123
Tru1I TTAA 3 cut(s) 159, 318, 647
Tru9I TTAA 3 cut(s) 159, 318, 647
TscAI CASTG 1 cut(s) 275
TspDTI ATGAA 3 cut(s) 115, 248, 486
TspRI CASTG 1 cut(s) 275
Vha464I CTTAAG 1 cut(s) 317
XceI RCATGY 2 cut(s) 290, 593
XspI CTAG 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.