Rh6CG032900

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0012696
Physical Location & Seq
Reverse (-)
3098412 .. 3099940
1529 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG032900.1

Sequence Viewer

Length: 498 bp
ATGTCCACATTCATAAATGTACAAAGAGAAACCGAGATCTTCCTGAAGCTTACAATTGACCGAGAGAACAGAAAGGTATTGTTTGCAGAAGCAAACAAGGATTTTGTTGACAATCTGGTGGGATTCATGATTTTCCCTACCTCTGCCATGATCACGCTTAACCCTGAACCTTTTGGAAAGCTAAACAATGTCAAGAAGAGCATTGAGAACATGACCGAGTATTACAATCCAATGAAGGCTTCTCTCCTGAATACAAAGCCACCAGCTCACTGCAATCGGCACGAGCATGCCCTGCATCCCCTTGATCCCTCATCACTTAAGAAGATGCCCTTTTATGTATGTGAAGAGTGTATTACTACCCGAGTGGCTGCGGGTTGTCCCGATCGGTCTTTGAAGGGGGTGCTGGTGGATTTGGCATGCTTGGCGTCGACGCTCGTGACAGCATTTGCAGTTCGGTCGGCTGCTTTCGAGGCTGAGGCCTGGGATAAGGACCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

165

Amino Acids

18.56

Weight (kDa)

6.51

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 8 - 90 1.5e-10 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 428
AciI CCGC 1 cut(s) 371
AclWI GGATC 1 cut(s) 299
AcuI CTGAAG 1 cut(s) 65
AcyI GRCGYC 1 cut(s) 425
AfaI GTAC 1 cut(s) 21
AflII CTTAAG 1 cut(s) 317
AgsI TTSAA 1 cut(s) 394
AjnI CCWGG 1 cut(s) 479
AjuI GAANNNNNNNTTGG 2 cut(s) 223, 255
AluBI AGCT 3 cut(s) 49, 181, 266
AluI AGCT 3 cut(s) 49, 181, 266
AlwI GGATC 1 cut(s) 299
Ama87I CYCGRG 1 cut(s) 360
AoxI GGCC 1 cut(s) 477
ApeKI GCWGC 2 cut(s) 368, 461
AspS9I GGNCC 1 cut(s) 490
AvaI CYCGRG 1 cut(s) 360
AvaII GGWCC 1 cut(s) 490
BauI CACGAG 2 cut(s) 281, 434
BbvCI CCTCAGC 1 cut(s) 474
BbvI GCAGC 2 cut(s) 355, 448
BcgI CGANNNNNNTGC 2 cut(s) 438, 472
BciT130I CCWGG 1 cut(s) 481
BclI TGATCA 1 cut(s) 150
BfrI CTTAAG 1 cut(s) 317
BglII AGATCT 1 cut(s) 36
BisI GCNGC 2 cut(s) 369, 462
BlsI GCNGC 2 cut(s) 370, 463
Bme1390I CCNGG 1 cut(s) 481
Bme18I GGWCC 1 cut(s) 490
BmeT110I CYCGRG 1 cut(s) 360
BmgT120I GGNCC 1 cut(s) 490
BmrFI CCNGG 1 cut(s) 481
BmsI GCATC 2 cut(s) 304, 315
Bpu10I CCTNAGC 1 cut(s) 474
BsaHI GRCGYC 1 cut(s) 425
BsaJI CCNNGG 1 cut(s) 480
BseBI CCWGG 1 cut(s) 481
BseDI CCNNGG 1 cut(s) 480
BseGI GGATG 1 cut(s) 295
BseMII CTCAG 1 cut(s) 465
BseXI GCAGC 2 cut(s) 355, 448
Bsh1285I CGRYCG 2 cut(s) 385, 458
BshFI GGCC 1 cut(s) 479
BsiEI CGRYCG 2 cut(s) 385, 458
BsiHKCI CYCGRG 1 cut(s) 360
BslFI GGGAC 1 cut(s) 363
BsmFI GGGAC 1 cut(s) 363
BsnI GGCC 1 cut(s) 479
BsoBI CYCGRG 1 cut(s) 360
Bsp1407I TGTACA 1 cut(s) 19
Bsp143I GATC 4 cut(s) 36, 150, 304, 382
BspACI CCGC 1 cut(s) 371
BspANI GGCC 1 cut(s) 479
BspCNI CTCAG 1 cut(s) 466
BspHI TCATGA 1 cut(s) 126
BspPI GGATC 1 cut(s) 299
BspQI GCTCTTC 1 cut(s) 191
BspTI CTTAAG 1 cut(s) 317
BsrGI TGTACA 1 cut(s) 19
BssECI CCNNGG 1 cut(s) 480
BssMI GATC 4 cut(s) 36, 150, 304, 382
BssNI GRCGYC 1 cut(s) 425
BssSI CACGAG 2 cut(s) 281, 434
Bst2BI CACGAG 2 cut(s) 281, 434
Bst2UI CCWGG 1 cut(s) 481
Bst6I CTCTTC 2 cut(s) 191, 339
BstACI GRCGYC 1 cut(s) 425
BstAFI CTTAAG 1 cut(s) 317
BstAPI GCANNNNNTGC 1 cut(s) 292
BstAUI TGTACA 1 cut(s) 19
BstC8I GCNNGC 2 cut(s) 288, 418
BstDEI CTNAG 1 cut(s) 474
BstF5I GGATG 1 cut(s) 295
BstKTI GATC 4 cut(s) 39, 153, 307, 385
BstMBI GATC 4 cut(s) 36, 150, 304, 382
BstMCI CGRYCG 2 cut(s) 385, 458
BstMWI GCNNNNNNNGC 3 cut(s) 292, 422, 470
BstNI CCWGG 1 cut(s) 481
BstNSI RCATGY 2 cut(s) 290, 420
BstSCI CCNGG 1 cut(s) 479
BstV1I GCAGC 2 cut(s) 355, 448
BstX2I RGATCY 1 cut(s) 36
BstYI RGATCY 1 cut(s) 36
BsuRI GGCC 1 cut(s) 479
BtsCI GGATG 1 cut(s) 295
BtsI GCAGTG 1 cut(s) 268
BtsIMutI CAGTG 1 cut(s) 268
Cac8I GCNNGC 2 cut(s) 288, 418
CciI TCATGA 1 cut(s) 126
Cfr13I GGNCC 1 cut(s) 490
CseI GACGC 2 cut(s) 414, 439
Csp6I GTAC 1 cut(s) 20
CviAII CATG 5 cut(s) 127, 148, 211, 287, 417
CviJI RGCY 9 cut(s) 49, 181, 239, 259, 266, 368, 461, 473, 479
CviKI_1 RGCY 9 cut(s) 49, 181, 239, 259, 266, 368, 461, 473, 479
CviQI GTAC 1 cut(s) 20
DdeI CTNAG 1 cut(s) 474
DpnI GATC 4 cut(s) 38, 152, 306, 384
DpnII GATC 4 cut(s) 36, 150, 304, 382
Eam1104I CTCTTC 2 cut(s) 191, 339
EarI CTCTTC 2 cut(s) 191, 339
Eco147I AGGCCT 1 cut(s) 479
Eco47I GGWCC 1 cut(s) 490
Eco57I CTGAAG 1 cut(s) 65
Eco88I CYCGRG 1 cut(s) 360
EcoRII CCWGG 1 cut(s) 479
FaeI CATG 5 cut(s) 130, 151, 214, 290, 420
FaiI YATR 8 cut(s) 14, 128, 149, 212, 288, 336, 340, 418
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FaqI GGGAC 1 cut(s) 363
FatI CATG 5 cut(s) 126, 147, 210, 286, 416
FauI CCCGC 1 cut(s) 364
FbaI TGATCA 1 cut(s) 150
FblI GTMKAC 1 cut(s) 428
Fnu4HI GCNGC 2 cut(s) 369, 462
FokI GGATG 1 cut(s) 282
Fsp4HI GCNGC 2 cut(s) 369, 462
GluI GCNGC 2 cut(s) 369, 462
HaeIII GGCC 1 cut(s) 479
HgaI GACGC 2 cut(s) 414, 439
Hin1I GRCGYC 1 cut(s) 425
Hin1II CATG 5 cut(s) 130, 151, 214, 290, 420
HincII GTYRAC 2 cut(s) 109, 429
HindII GTYRAC 2 cut(s) 109, 429
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 1 cut(s) 123
Hpy166II GTNNAC 3 cut(s) 6, 109, 429
Hpy188III TCNNGA 6 cut(s) 43, 127, 193, 247, 380, 436
Hpy8I GTNNAC 3 cut(s) 6, 109, 429
Hpy99I CGWCG 2 cut(s) 430, 433
HpyAV CCTTC 2 cut(s) 229, 388
HpyCH4V TGCA 4 cut(s) 86, 273, 295, 449
HpyF10VI GCNNNNNNNGC 3 cut(s) 292, 422, 470
HpyF3I CTNAG 1 cut(s) 474
Hsp92I GRCGYC 1 cut(s) 425
Hsp92II CATG 5 cut(s) 130, 151, 214, 290, 420
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 4 cut(s) 36, 150, 304, 382
LguI GCTCTTC 1 cut(s) 191
LpnPI CCDG 9 cut(s) 56, 101, 177, 260, 276, 305, 389, 466, 493
Lsp1109I GCAGC 2 cut(s) 355, 448
LweI GCATC 2 cut(s) 304, 315
MaeIII GTNAC 1 cut(s) 436
MalI GATC 4 cut(s) 38, 152, 306, 384
MboI GATC 4 cut(s) 36, 150, 304, 382
MboII GAAGA 4 cut(s) 31, 208, 334, 356
MfeI CAATTG 1 cut(s) 54
MflI RGATCY 1 cut(s) 36
MluCI AATT 1 cut(s) 54
MnlI CCTC 4 cut(s) 151, 319, 463, 469
MseI TTAA 2 cut(s) 159, 318
MslI CAYNNNNRTG 1 cut(s) 285
MspCI CTTAAG 1 cut(s) 317
MspR9I CCNGG 1 cut(s) 481
MunI CAATTG 1 cut(s) 54
MvaI CCWGG 1 cut(s) 481
MwoI GCNNNNNNNGC 3 cut(s) 292, 422, 470
NdeII GATC 4 cut(s) 36, 150, 304, 382
NlaIII CATG 5 cut(s) 130, 151, 214, 290, 420
NmuCI GTSAC 1 cut(s) 436
NspI RCATGY 2 cut(s) 290, 420
PaeI GCATGC 2 cut(s) 290, 420
PagI TCATGA 1 cut(s) 126
PceI AGGCCT 1 cut(s) 479
PciSI GCTCTTC 1 cut(s) 191
PfeI GAWTC 1 cut(s) 123
PkrI GCNGC 2 cut(s) 370, 463
Ple19I CGATCG 1 cut(s) 385
Psp6I CCWGG 1 cut(s) 479
PspGI CCWGG 1 cut(s) 479
PspPI GGNCC 1 cut(s) 490
PsuI RGATCY 1 cut(s) 36
PvuI CGATCG 1 cut(s) 385
RsaI GTAC 1 cut(s) 21
RsaNI GTAC 1 cut(s) 20
RseI CAYNNNNRTG 1 cut(s) 285
SalI GTCGAC 1 cut(s) 427
SapI GCTCTTC 1 cut(s) 191
SaqAI TTAA 2 cut(s) 159, 318
SatI GCNGC 2 cut(s) 369, 462
Sau3AI GATC 4 cut(s) 36, 150, 304, 382
Sau96I GGNCC 1 cut(s) 490
ScrFI CCNGG 1 cut(s) 481
SetI ASST 6 cut(s) 51, 78, 143, 172, 183, 268
SfaNI GCATC 2 cut(s) 304, 315
SgrDI CGTCGACG 1 cut(s) 427
SinI GGWCC 1 cut(s) 490
SmiMI CAYNNNNRTG 1 cut(s) 285
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
SphI GCATGC 2 cut(s) 290, 420
Sse9I AATT 1 cut(s) 54
SseBI AGGCCT 1 cut(s) 479
SsiI CCGC 1 cut(s) 371
StuI AGGCCT 1 cut(s) 479
StyD4I CCNGG 1 cut(s) 479
TaqI TCGA 2 cut(s) 428, 468
TaqII GACCGA 4 cut(s) 75, 230, 375, 444
TasI AATT 1 cut(s) 54
TatI WGTACW 1 cut(s) 19
TfiI GAWTC 1 cut(s) 123
Tru1I TTAA 2 cut(s) 159, 318
Tru9I TTAA 2 cut(s) 159, 318
TscAI CASTG 1 cut(s) 275
TseFI GTSAC 1 cut(s) 436
TseI GCWGC 2 cut(s) 368, 461
Tsp45I GTSAC 1 cut(s) 436
TspDTI ATGAA 2 cut(s) 115, 248
TspRI CASTG 1 cut(s) 275
Vha464I CTTAAG 1 cut(s) 317
VpaK11BI GGWCC 1 cut(s) 490
XceI RCATGY 2 cut(s) 290, 420
XmiI GTMKAC 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.