RLG00000015226

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0012696
Physical Location & Seq
Forward (+)
63358364 .. 63360449
2086 bp
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UTR
Exon/CDS
Intron
RLM00000015226

Sequence Viewer

Length: 486 bp
ATGGAGATCACCGGTGTTGGAGTCGTCAATGGTCTTGGTGTACCAATCAAGGCCGTTGGAGCAAAACTACTTGCTAGAGGTTCTCAACGCAGCCATCAACTCATCATGTCCACATTTTATGACCCAGAGGAAGAAGCTAAGATCTTCCTTAGGCTTACGATTCTGAGAGAGAACAAAAAGGTCTTGTTTGCCAAAGCAAACAAAGATTTCGTCGACAACCTGCTCGGATTAATGACTTTCCCCGTCTCTTCAATGATTCCTTTTATCCCCGAAGCTTTTGGGAGTCTAACTAATGTGAGGAAGAGCATCGAGAAGATGGCAAAATATCACAATCCGATGAAGGAATTGCTTCTGAATACTAAGCCACCAGCTCATCGAAACTGTAAACAGGGGTGCAAATATAAATCATCGGTGATAATATATTTGGATTCGTACAAGACGAAACCTCCTTCTTTATTAGCTCTTTCACTTGCGGTGCCACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

162

Amino Acids

17.91

Weight (kDa)

9.78

Isoelectric Point (pI)

49.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 45 - 130 9.8e-10 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 228
AccB1I GGYRCC 1 cut(s) 475
AccI GTMKAC 1 cut(s) 213
AciI CCGC 1 cut(s) 473
AfaI GTAC 2 cut(s) 42, 434
AgeI ACCGGT 1 cut(s) 11
AgsI TTSAA 1 cut(s) 252
AluBI AGCT 4 cut(s) 137, 275, 371, 461
AluI AGCT 4 cut(s) 137, 275, 371, 461
Alw26I GTCTC 1 cut(s) 250
AoxI GGCC 1 cut(s) 51
ApeKI GCWGC 1 cut(s) 90
ArsI GACNNNNNNTTYG 2 cut(s) 195, 227
AseI ATTAAT 1 cut(s) 230
AsiGI ACCGGT 1 cut(s) 11
Asp700I GAANNNNTTC 1 cut(s) 348
AsuHPI GGTGA 1 cut(s) 424
AxyI CCTNAGG 1 cut(s) 149
BanI GGYRCC 1 cut(s) 475
BbvI GCAGC 1 cut(s) 102
BccI CCATC 2 cut(s) 102, 310
BceAI ACGGC 1 cut(s) 38
BcoDI GTCTC 1 cut(s) 250
BfaI CTAG 1 cut(s) 75
BfuAI ACCTGC 1 cut(s) 228
BglII AGATCT 1 cut(s) 141
BisI GCNGC 1 cut(s) 91
BlsI GCNGC 1 cut(s) 92
BmiI GGNNCC 1 cut(s) 477
BmsI GCATC 1 cut(s) 315
BsaWI WCCGGW 1 cut(s) 11
BsaXI ACNNNNNCTCC 2 cut(s) 430, 460
Bse118I RCCGGY 1 cut(s) 11
Bse21I CCTNAGG 1 cut(s) 149
BseMII CTCAG 1 cut(s) 155
BseXI GCAGC 1 cut(s) 102
BshFI GGCC 1 cut(s) 53
BshNI GGYRCC 1 cut(s) 475
BshTI ACCGGT 1 cut(s) 11
BsiSI CCGG 1 cut(s) 12
BsmAI GTCTC 1 cut(s) 250
BsmBI CGTCTC 1 cut(s) 250
BsnI GGCC 1 cut(s) 53
Bsp143I GATC 2 cut(s) 6, 141
BspACI CCGC 1 cut(s) 473
BspANI GGCC 1 cut(s) 53
BspCNI CTCAG 1 cut(s) 156
BspLI GGNNCC 1 cut(s) 477
BspMI ACCTGC 1 cut(s) 228
BspQI GCTCTTC 1 cut(s) 296
BspT107I GGYRCC 1 cut(s) 475
BsrFI RCCGGY 1 cut(s) 11
BssAI RCCGGY 1 cut(s) 11
BssMI GATC 2 cut(s) 6, 141
Bst4CI ACNGT 1 cut(s) 383
Bst6I CTCTTC 2 cut(s) 253, 296
BstDEI CTNAG 4 cut(s) 138, 149, 164, 360
BstKTI GATC 2 cut(s) 9, 144
BstMAI GTCTC 1 cut(s) 250
BstMBI GATC 2 cut(s) 6, 141
BstMWI GCNNNNNNNGC 1 cut(s) 59
BstV1I GCAGC 1 cut(s) 102
BstX2I RGATCY 1 cut(s) 141
BstYI RGATCY 1 cut(s) 141
Bsu36I CCTNAGG 1 cut(s) 149
BsuRI GGCC 1 cut(s) 53
BveI ACCTGC 1 cut(s) 228
Cfr10I RCCGGY 1 cut(s) 11
Csp6I GTAC 2 cut(s) 41, 433
CspAI ACCGGT 1 cut(s) 11
CviAII CATG 1 cut(s) 106
CviJI RGCY 8 cut(s) 53, 93, 137, 154, 275, 364, 371, 461
CviKI_1 RGCY 8 cut(s) 53, 93, 137, 154, 275, 364, 371, 461
CviQI GTAC 2 cut(s) 41, 433
DdeI CTNAG 4 cut(s) 138, 149, 164, 360
DpnI GATC 2 cut(s) 8, 143
DpnII GATC 2 cut(s) 6, 141
Eam1104I CTCTTC 2 cut(s) 253, 296
EarI CTCTTC 2 cut(s) 253, 296
Eco81I CCTNAGG 1 cut(s) 149
Esp3I CGTCTC 1 cut(s) 250
FaeI CATG 1 cut(s) 109
FaiI YATR 4 cut(s) 107, 120, 402, 421
FatI CATG 1 cut(s) 105
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 1 cut(s) 91
Fsp4HI GCNGC 1 cut(s) 91
FspBI CTAG 1 cut(s) 75
GluI GCNGC 1 cut(s) 91
HaeIII GGCC 1 cut(s) 53
HapII CCGG 1 cut(s) 12
Hin1II CATG 1 cut(s) 109
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HindIII AAGCTT 1 cut(s) 273
HinfI GANTC 5 cut(s) 21, 160, 256, 283, 428
HpaII CCGG 1 cut(s) 12
HphI GGTGA 1 cut(s) 424
Hpy166II GTNNAC 4 cut(s) 41, 111, 214, 386
Hpy188I TCNGA 4 cut(s) 165, 227, 336, 354
Hpy188III TCNNGA 1 cut(s) 310
Hpy8I GTNNAC 4 cut(s) 41, 111, 214, 386
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 2 cut(s) 334, 459
HpyCH4III ACNGT 1 cut(s) 383
HpyCH4V TGCA 1 cut(s) 396
HpyF10VI GCNNNNNNNGC 1 cut(s) 59
HpyF3I CTNAG 4 cut(s) 138, 149, 164, 360
Hsp92II CATG 1 cut(s) 109
Kzo9I GATC 2 cut(s) 6, 141
LguI GCTCTTC 1 cut(s) 296
LmnI GCTCC 1 cut(s) 59
LpnPI CCDG 5 cut(s) 25, 138, 233, 374, 381
Lsp1109I GCAGC 1 cut(s) 102
LweI GCATC 1 cut(s) 315
MaeI CTAG 1 cut(s) 75
MalI GATC 2 cut(s) 8, 143
MboI GATC 2 cut(s) 6, 141
MboII GAAGA 5 cut(s) 136, 143, 240, 313, 325
MflI RGATCY 1 cut(s) 141
MluCI AATT 1 cut(s) 344
MlyI GAGTC 2 cut(s) 30, 292
MmeI TCCRAC 1 cut(s) 37
MnlI CCTC 4 cut(s) 71, 121, 291, 456
MroXI GAANNNNTTC 1 cut(s) 348
MseI TTAA 1 cut(s) 230
MspI CCGG 1 cut(s) 12
MwoI GCNNNNNNNGC 1 cut(s) 59
NdeII GATC 2 cut(s) 6, 141
NlaIII CATG 1 cut(s) 109
NlaIV GGNNCC 1 cut(s) 477
PciSI GCTCTTC 1 cut(s) 296
PcsI WCGNNNNNNNCGW 1 cut(s) 437
PdmI GAANNNNTTC 1 cut(s) 348
PfeI GAWTC 3 cut(s) 160, 256, 428
PinAI ACCGGT 1 cut(s) 11
PkrI GCNGC 1 cut(s) 92
PleI GAGTC 2 cut(s) 29, 291
PpsI GAGTC 2 cut(s) 29, 291
PshBI ATTAAT 1 cut(s) 230
PspN4I GGNNCC 1 cut(s) 477
PsuI RGATCY 1 cut(s) 141
RsaI GTAC 2 cut(s) 42, 434
RsaNI GTAC 2 cut(s) 41, 433
SalI GTCGAC 1 cut(s) 212
SapI GCTCTTC 1 cut(s) 296
SaqAI TTAA 1 cut(s) 230
SatI GCNGC 1 cut(s) 91
Sau3AI GATC 2 cut(s) 6, 141
SchI GAGTC 2 cut(s) 30, 292
SetI ASST 8 cut(s) 82, 139, 183, 222, 277, 373, 448, 463
SfaNI GCATC 1 cut(s) 315
SgrAI CRCCGGYG 1 cut(s) 11
Sse9I AATT 1 cut(s) 344
SsiI CCGC 1 cut(s) 473
SspMI CTAG 1 cut(s) 75
TaaI ACNGT 1 cut(s) 383
TaqI TCGA 3 cut(s) 213, 309, 376
TasI AATT 1 cut(s) 344
TfiI GAWTC 3 cut(s) 160, 256, 428
Tru1I TTAA 1 cut(s) 230
Tru9I TTAA 1 cut(s) 230
TseI GCWGC 1 cut(s) 90
TspDTI ATGAA 1 cut(s) 353
VspI ATTAAT 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 213
XmnI GAANNNNTTC 1 cut(s) 348
XspI CTAG 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.