Rroxscaffold_7G00214380

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0012696
Physical Location & Seq
Forward (+)
65015097 .. 65016573
1477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00214380.1

Sequence Viewer

Length: 744 bp
ATGTCCACATTCATAAATGTACAAAGAGAAACCGAGATCTTCCTGAAGCTTACAATTGACCGAGAGAACAGAAAGGTATTGTTTGCAGAAGCAAACAAGGATTTTGTTGACAATCTGGTGGGATTCATGATTTTCCCTACCTCTGCCATGATCACGCTTAACCCTGAACCTTTTGGAAAGCTAAACAATGTCAAGAAGAGCATTGAGAACATGACCGAGTATTACAATCCAATGAAGGCTTCTCTCCTGAATACAAAGCCACCAGCTCACTGCAATCGGCACGAGCATGCCCTGCATCCCCTTGATCCCTCATCACTTAAGAAGATGCCCTTTTATGTATGTGAAGAGTGTATTACTACCCGAGGTAAGCCATACTATGTGGCCGACGATCCCGCAGTCTTGTGTCCTACTTGTAACCATGCCATGACTCAGGGTGCAAGTTATTGTGGTACACCTATTGAAATCGCCAATGAAGGGGGAGGTTTTGTTCGACAGCGAGCGAAATACATCATTACTGATGATTTGAATATTCACCCTCTTCTCACGGTTTCTCTCACCACAAACATAATTCGTGGAGCTAAACCTCAGCATGTTGAAGAGGTTACCGTGAACGTTTCTCATAAGAAGTTGGTGTTGATGGGGGTGGTGGTGGATGTGTTCTATAGTGGCTGCGGGTTGTCCCGATCGGTCTTTGAAGGGGGTGCTGGTAGATTTGGCATGCTTGGCGTCGACGCTCGATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

247

Amino Acids

27.43

Weight (kDa)

7.63

Isoelectric Point (pI)

40.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 10 - 211 4.2e-18 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 729
AciI CCGC 2 cut(s) 393, 672
AclI AACGTT 1 cut(s) 612
AclWI GGATC 2 cut(s) 299, 383
AcoI YGGCCR 1 cut(s) 381
AcuI CTGAAG 1 cut(s) 65
AcyI GRCGYC 1 cut(s) 726
AfaI GTAC 2 cut(s) 21, 451
AfiI CCNNNNNNNGG 1 cut(s) 474
AflII CTTAAG 1 cut(s) 317
AgsI TTSAA 4 cut(s) 461, 526, 596, 695
AjuI GAANNNNNNNTTGG 2 cut(s) 223, 255
AloI GAACNNNNNNTCC 2 cut(s) 471, 503
AluBI AGCT 4 cut(s) 49, 181, 266, 578
AluI AGCT 4 cut(s) 49, 181, 266, 578
AlwI GGATC 2 cut(s) 299, 383
Ama87I CYCGRG 1 cut(s) 360
AoxI GGCC 1 cut(s) 381
ApeKI GCWGC 1 cut(s) 669
AsuHPI GGTGA 2 cut(s) 524, 547
AvaI CYCGRG 1 cut(s) 360
BauI CACGAG 1 cut(s) 281
BbvCI CCTCAGC 1 cut(s) 585
BbvI GCAGC 1 cut(s) 656
BccI CCATC 1 cut(s) 631
BclI TGATCA 1 cut(s) 150
BfmI CTRYAG 1 cut(s) 661
BfrI CTTAAG 1 cut(s) 317
BglII AGATCT 1 cut(s) 36
BisI GCNGC 1 cut(s) 670
BlsI GCNGC 1 cut(s) 671
BmeT110I CYCGRG 1 cut(s) 360
BmsI GCATC 2 cut(s) 304, 315
Bpu10I CCTNAGC 1 cut(s) 585
BsaHI GRCGYC 1 cut(s) 726
BsaJI CCNNGG 1 cut(s) 361
BsaXI ACNNNNNCTCC 2 cut(s) 471, 501
Bsc4I CCNNNNNNNGG 1 cut(s) 474
BseDI CCNNGG 1 cut(s) 361
BseGI GGATG 2 cut(s) 295, 658
BseLI CCNNNNNNNGG 1 cut(s) 474
BseMII CTCAG 2 cut(s) 443, 599
BseXI GCAGC 1 cut(s) 656
Bsh1285I CGRYCG 2 cut(s) 686, 740
BshFI GGCC 1 cut(s) 383
BsiEI CGRYCG 2 cut(s) 686, 740
BsiHKCI CYCGRG 1 cut(s) 360
BslFI GGGAC 1 cut(s) 664
BslI CCNNNNNNNGG 1 cut(s) 474
BsmFI GGGAC 1 cut(s) 664
BsnI GGCC 1 cut(s) 383
BsoBI CYCGRG 1 cut(s) 360
Bsp1407I TGTACA 1 cut(s) 19
Bsp143I GATC 6 cut(s) 36, 150, 304, 388, 683, 737
BspACI CCGC 2 cut(s) 393, 672
BspANI GGCC 1 cut(s) 383
BspCNI CTCAG 2 cut(s) 442, 598
BspHI TCATGA 1 cut(s) 126
BspPI GGATC 2 cut(s) 299, 383
BspQI GCTCTTC 1 cut(s) 191
BspTI CTTAAG 1 cut(s) 317
BsrGI TGTACA 1 cut(s) 19
BssECI CCNNGG 1 cut(s) 361
BssMI GATC 6 cut(s) 36, 150, 304, 388, 683, 737
BssNI GRCGYC 1 cut(s) 726
BssSI CACGAG 1 cut(s) 281
Bst2BI CACGAG 1 cut(s) 281
Bst4CI ACNGT 2 cut(s) 547, 607
Bst6I CTCTTC 4 cut(s) 191, 339, 543, 591
BstACI GRCGYC 1 cut(s) 726
BstAFI CTTAAG 1 cut(s) 317
BstAPI GCANNNNNTGC 1 cut(s) 292
BstAUI TGTACA 1 cut(s) 19
BstC8I GCNNGC 3 cut(s) 288, 498, 719
BstDEI CTNAG 2 cut(s) 429, 585
BstEII GGTNACC 1 cut(s) 601
BstF5I GGATG 2 cut(s) 295, 658
BstKTI GATC 6 cut(s) 39, 153, 307, 391, 686, 740
BstMBI GATC 6 cut(s) 36, 150, 304, 388, 683, 737
BstMCI CGRYCG 2 cut(s) 686, 740
BstMWI GCNNNNNNNGC 2 cut(s) 292, 723
BstNSI RCATGY 3 cut(s) 290, 593, 721
BstPI GGTNACC 1 cut(s) 601
BstSFI CTRYAG 1 cut(s) 661
BstV1I GCAGC 1 cut(s) 656
BstX2I RGATCY 1 cut(s) 36
BstYI RGATCY 1 cut(s) 36
BsuRI GGCC 1 cut(s) 383
BtsCI GGATG 2 cut(s) 295, 658
BtsI GCAGTG 1 cut(s) 268
BtsIMutI CAGTG 1 cut(s) 268
Cac8I GCNNGC 3 cut(s) 288, 498, 719
CciI TCATGA 1 cut(s) 126
CseI GACGC 1 cut(s) 715
Csp6I GTAC 2 cut(s) 20, 450
CviAII CATG 8 cut(s) 127, 148, 211, 287, 419, 424, 590, 718
CviJI RGCY 9 cut(s) 49, 181, 239, 259, 266, 370, 383, 578, 669
CviKI_1 RGCY 9 cut(s) 49, 181, 239, 259, 266, 370, 383, 578, 669
CviQI GTAC 2 cut(s) 20, 450
DdeI CTNAG 2 cut(s) 429, 585
DpnI GATC 6 cut(s) 38, 152, 306, 390, 685, 739
DpnII GATC 6 cut(s) 36, 150, 304, 388, 683, 737
EaeI YGGCCR 1 cut(s) 381
Eam1104I CTCTTC 4 cut(s) 191, 339, 543, 591
EarI CTCTTC 4 cut(s) 191, 339, 543, 591
Eco57I CTGAAG 1 cut(s) 65
Eco88I CYCGRG 1 cut(s) 360
Eco91I GGTNACC 1 cut(s) 601
EcoO65I GGTNACC 1 cut(s) 601
FaeI CATG 8 cut(s) 130, 151, 214, 290, 422, 427, 593, 721
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FaqI GGGAC 1 cut(s) 664
FatI CATG 8 cut(s) 126, 147, 210, 286, 418, 423, 589, 717
FauI CCCGC 2 cut(s) 400, 665
FbaI TGATCA 1 cut(s) 150
FblI GTMKAC 1 cut(s) 729
Fnu4HI GCNGC 1 cut(s) 670
FokI GGATG 2 cut(s) 282, 665
Fsp4HI GCNGC 1 cut(s) 670
GluI GCNGC 1 cut(s) 670
HaeIII GGCC 1 cut(s) 383
HgaI GACGC 1 cut(s) 715
Hin1I GRCGYC 1 cut(s) 726
Hin1II CATG 8 cut(s) 130, 151, 214, 290, 422, 427, 593, 721
HincII GTYRAC 2 cut(s) 109, 730
HindII GTYRAC 2 cut(s) 109, 730
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 2 cut(s) 123, 427
HphI GGTGA 2 cut(s) 524, 547
Hpy166II GTNNAC 5 cut(s) 6, 109, 452, 610, 730
Hpy188III TCNNGA 6 cut(s) 43, 127, 193, 247, 681, 741
Hpy8I GTNNAC 5 cut(s) 6, 109, 452, 610, 730
Hpy99I CGWCG 3 cut(s) 389, 731, 734
HpyAV CCTTC 3 cut(s) 229, 467, 689
HpyCH4III ACNGT 2 cut(s) 547, 607
HpyCH4IV ACGT 1 cut(s) 612
HpyCH4V TGCA 4 cut(s) 86, 273, 295, 437
HpyF10VI GCNNNNNNNGC 2 cut(s) 292, 723
HpyF3I CTNAG 2 cut(s) 429, 585
HpySE526I ACGT 1 cut(s) 612
Hsp92I GRCGYC 1 cut(s) 726
Hsp92II CATG 8 cut(s) 130, 151, 214, 290, 422, 427, 593, 721
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 6 cut(s) 36, 150, 304, 388, 683, 737
LguI GCTCTTC 1 cut(s) 191
LmnI GCTCC 1 cut(s) 575
LpnPI CCDG 8 cut(s) 56, 101, 177, 260, 276, 305, 416, 690
Lsp1109I GCAGC 1 cut(s) 656
LweI GCATC 2 cut(s) 304, 315
MaeII ACGT 1 cut(s) 612
MaeIII GTNAC 2 cut(s) 413, 601
MalI GATC 6 cut(s) 38, 152, 306, 390, 685, 739
MboI GATC 6 cut(s) 36, 150, 304, 388, 683, 737
MboII GAAGA 6 cut(s) 31, 208, 334, 356, 530, 608
MfeI CAATTG 1 cut(s) 54
MflI RGATCY 1 cut(s) 36
MluCI AATT 2 cut(s) 54, 567
MlyI GAGTC 1 cut(s) 421
MnlI CCTC 7 cut(s) 151, 319, 356, 473, 546, 592, 594
MseI TTAA 2 cut(s) 159, 318
MslI CAYNNNNRTG 1 cut(s) 285
MspCI CTTAAG 1 cut(s) 317
MunI CAATTG 1 cut(s) 54
MwoI GCNNNNNNNGC 2 cut(s) 292, 723
NdeII GATC 6 cut(s) 36, 150, 304, 388, 683, 737
NlaIII CATG 8 cut(s) 130, 151, 214, 290, 422, 427, 593, 721
NspI RCATGY 3 cut(s) 290, 593, 721
PaeI GCATGC 2 cut(s) 290, 721
PagI TCATGA 1 cut(s) 126
PciSI GCTCTTC 1 cut(s) 191
PfeI GAWTC 1 cut(s) 123
PkrI GCNGC 1 cut(s) 671
Ple19I CGATCG 2 cut(s) 686, 740
PleI GAGTC 1 cut(s) 421
PpsI GAGTC 1 cut(s) 421
Psp1406I AACGTT 1 cut(s) 612
PspEI GGTNACC 1 cut(s) 601
PsuI RGATCY 1 cut(s) 36
PvuI CGATCG 2 cut(s) 686, 740
RsaI GTAC 2 cut(s) 21, 451
RsaNI GTAC 2 cut(s) 20, 450
RseI CAYNNNNRTG 1 cut(s) 285
SalI GTCGAC 1 cut(s) 728
SapI GCTCTTC 1 cut(s) 191
SaqAI TTAA 2 cut(s) 159, 318
SatI GCNGC 1 cut(s) 670
Sau3AI GATC 6 cut(s) 36, 150, 304, 388, 683, 737
SchI GAGTC 1 cut(s) 421
SfaNI GCATC 2 cut(s) 304, 315
SfcI CTRYAG 1 cut(s) 661
SgrDI CGTCGACG 1 cut(s) 728
SmiMI CAYNNNNRTG 1 cut(s) 285
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
SphI GCATGC 2 cut(s) 290, 721
Sse9I AATT 2 cut(s) 54, 567
SsiI CCGC 2 cut(s) 393, 672
SspI AATATT 1 cut(s) 529
TaaI ACNGT 2 cut(s) 547, 607
TaiI ACGT 1 cut(s) 615
TaqI TCGA 3 cut(s) 490, 729, 736
TaqII GACCGA 3 cut(s) 75, 230, 676
TasI AATT 2 cut(s) 54, 567
TatI WGTACW 1 cut(s) 19
TfiI GAWTC 1 cut(s) 123
Tru1I TTAA 2 cut(s) 159, 318
Tru9I TTAA 2 cut(s) 159, 318
TscAI CASTG 1 cut(s) 275
TseI GCWGC 1 cut(s) 669
TspDTI ATGAA 3 cut(s) 115, 248, 486
TspRI CASTG 1 cut(s) 275
Vha464I CTTAAG 1 cut(s) 317
XceI RCATGY 3 cut(s) 290, 593, 721
XmiI GTMKAC 1 cut(s) 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.