RLG00000016648

TIFY 3B-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011174
Physical Location & Seq
Forward (+)
8569286 .. 8570945
1660 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016648

Sequence Viewer

Length: 615 bp
ATGGCTGAAAAACTTGAGCTTGGTGATGTGAAACAGCCCAAAGAAGAAGCTGAGGAAATTGAGGCCTCTACTCATGATCTGCCCAACATTAATAGCAACAGAAACAAGCCATCTGCACTTAATATCCCTGCTCGTGCTCCTGCTCAGCTTACCATCTTCTACGCAGGGAGTGTTAGCGTCTTTGATGCAGTTACTGCAGAAAAGGTCCGTGAACTTCTTATCGCGGCTGCTGCAGCTGCTGCTGATAAGGTCATTGATGTAAATAATAGTGGCAACAGCTGTCCTCCAAGTCCACAGATACAAACCGGGTCGAGTTCTCTTCAAAATTCTTCTACTGCTCCTGGTTCACCAGTTGCCCAGCTCTACCCTGACCAGAAGAGCTCCATTTGCAAATTGCAAGCTGAATTCCCAATTGCAAGAAGACACTCTCTTCAGCGTTTCCTGGAGAAGCGTAGGGACAGATTGGGCAGCAAGAGTCCATACGCTACTTCTCCAGCAACACCGGTGGATGATAATGCAAAGACAAACCTCAGCAATAATGCTTCTTCCGGTTTGGGTTGTTTCAAACAATCTGCAGTGGCTCAGGAACAAATTCAACCAAGTTCTGCGGCCTAG

Protein Analysis

205

Amino Acids

21.6

Weight (kDa)

6.74

Isoelectric Point (pI)

71.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tify PF06200 46 - 76 1e-13 tify domain
Jas_motif PF09425 137 - 161 2.2e-14 Jas motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011174)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G43440 AT3G43440 AT5G20900
fragaria_vesca FvH4_1g09690
malus_domestica MD02G1105900.v1.1 MD15G1225800.v1.1
prunus_persica Prupe.7G189200_v2.0.a1
pyrus_communis pycom02g08380 pycom15g20070
rosa_chinensis RchiOBHm_Chr2g0096551
rosa_laevigata RLG00000016648
rosa_multiflora Rmu_sc0004096.1_g000006
rosa_roxburghii Rroxscaffold_2G00145540
rosa_rugosa Rorug02G0058400 Rorug02G0058400
rosa_samantha Rh2AG105600 Rh2BG108100 Rh2CG109900 Rh2DG108700
rosa_wichuraiana Rw2G008170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 224
AciI CCGC 2 cut(s) 224, 608
AcsI RAATTY 3 cut(s) 325, 404, 591
AcuI CTGAAG 1 cut(s) 416
AgeI ACCGGT 1 cut(s) 502
AgsI TTSAA 3 cut(s) 323, 565, 596
AjnI CCWGG 2 cut(s) 340, 441
AluBI AGCT 8 cut(s) 19, 50, 148, 236, 279, 361, 381, 401
AluI AGCT 8 cut(s) 19, 50, 148, 236, 279, 361, 381, 401
Alw21I GWGCWC 2 cut(s) 139, 383
AlwNI CAGNNNCTG 2 cut(s) 194, 239
AoxI GGCC 2 cut(s) 63, 609
ApeKI GCWGC 6 cut(s) 227, 230, 233, 236, 239, 468
ApoI RAATTY 3 cut(s) 325, 404, 591
AseI ATTAAT 1 cut(s) 90
AsiGI ACCGGT 1 cut(s) 502
Asp700I GAANNNNTTC 1 cut(s) 591
AspS9I GGNCC 1 cut(s) 205
AsuC2I CCSGG 1 cut(s) 307
AsuHPI GGTGA 2 cut(s) 35, 339
AvaII GGWCC 1 cut(s) 205
BanII GRGCYC 1 cut(s) 383
BauI CACGAG 1 cut(s) 132
BbsI GAAGAC 1 cut(s) 427
Bbv12I GWGCWC 2 cut(s) 139, 383
BbvCI CCTCAGC 2 cut(s) 51, 530
BbvI GCAGC 6 cut(s) 214, 217, 223, 226, 245, 480
BccI CCATC 2 cut(s) 118, 161
BciT130I CCWGG 2 cut(s) 342, 443
BcnI CCSGG 1 cut(s) 307
BfaI CTAG 1 cut(s) 613
BfmI CTRYAG 3 cut(s) 195, 231, 573
BisI GCNGC 8 cut(s) 225, 228, 231, 234, 237, 240, 469, 609
BlpI GCTNAGC 1 cut(s) 144
BlsI GCNGC 8 cut(s) 226, 229, 232, 235, 238, 241, 470, 610
Bme1390I CCNGG 3 cut(s) 307, 342, 443
Bme18I GGWCC 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 205
BmrFI CCNGG 3 cut(s) 307, 342, 443
BmsI GCATC 1 cut(s) 175
BpiI GAAGAC 1 cut(s) 427
BpmI CTGGAG 2 cut(s) 464, 477
Bpu10I CCTNAGC 3 cut(s) 51, 530, 582
Bpu1102I GCTNAGC 1 cut(s) 144
BpuEI CTTGAG 1 cut(s) 35
BpuMI CCSGG 1 cut(s) 307
BsaWI WCCGGW 2 cut(s) 502, 548
Bse118I RCCGGY 1 cut(s) 502
Bse1I ACTGG 1 cut(s) 350
BseBI CCWGG 2 cut(s) 342, 443
BseGI GGATG 1 cut(s) 514
BseMII CTCAG 4 cut(s) 42, 158, 544, 596
BseNI ACTGG 1 cut(s) 350
BseXI GCAGC 6 cut(s) 214, 217, 223, 226, 245, 480
BseYI CCCAGC 1 cut(s) 357
BsgI GTGCAG 1 cut(s) 99
Bsh1236I CGCG 1 cut(s) 224
BshFI GGCC 2 cut(s) 65, 611
BshTI ACCGGT 1 cut(s) 502
BsiHKAI GWGCWC 2 cut(s) 139, 383
BsiSI CCGG 3 cut(s) 306, 503, 549
BslFI GGGAC 1 cut(s) 470
BsmFI GGGAC 1 cut(s) 470
BsnI GGCC 2 cut(s) 65, 611
Bsp1286I GDGCHC 2 cut(s) 139, 383
Bsp143I GATC 1 cut(s) 76
Bsp1720I GCTNAGC 1 cut(s) 144
BspACI CCGC 2 cut(s) 224, 608
BspANI GGCC 2 cut(s) 65, 611
BspCNI CTCAG 4 cut(s) 43, 157, 543, 595
BspFNI CGCG 1 cut(s) 224
BspHI TCATGA 1 cut(s) 73
BspMAI CTGCAG 3 cut(s) 199, 235, 577
BspQI GCTCTTC 1 cut(s) 371
BsrFI RCCGGY 1 cut(s) 502
BsrI ACTGG 1 cut(s) 350
BssAI RCCGGY 1 cut(s) 502
BssMI GATC 1 cut(s) 76
BssSI CACGAG 1 cut(s) 132
Bst2BI CACGAG 1 cut(s) 132
Bst2UI CCWGG 2 cut(s) 342, 443
Bst6I CTCTTC 3 cut(s) 324, 371, 435
BstAPI GCANNNNNTGC 2 cut(s) 194, 239
BstC8I GCNNGC 1 cut(s) 399
BstDEI CTNAG 4 cut(s) 51, 144, 530, 582
BstF5I GGATG 1 cut(s) 514
BstFNI CGCG 1 cut(s) 224
BstKTI GATC 1 cut(s) 79
BstMBI GATC 1 cut(s) 76
BstMWI GCNNNNNNNGC 6 cut(s) 194, 230, 233, 236, 239, 387
BstNI CCWGG 2 cut(s) 342, 443
BstSCI CCNGG 3 cut(s) 305, 340, 441
BstSFI CTRYAG 3 cut(s) 195, 231, 573
BstUI CGCG 1 cut(s) 224
BstV1I GCAGC 6 cut(s) 214, 217, 223, 226, 245, 480
BstV2I GAAGAC 1 cut(s) 427
BsuRI GGCC 2 cut(s) 65, 611
BtsCI GGATG 1 cut(s) 514
BtsI GCAGTG 1 cut(s) 582
BtsIMutI CAGTG 1 cut(s) 582
Cac8I GCNNGC 1 cut(s) 399
CaiI CAGNNNCTG 2 cut(s) 194, 239
CciI TCATGA 1 cut(s) 73
Cfr10I RCCGGY 1 cut(s) 502
Cfr13I GGNCC 1 cut(s) 205
CseI GACGC 1 cut(s) 166
CspAI ACCGGT 1 cut(s) 502
CspCI CAANNNNNGTGG 2 cut(s) 486, 521
CviAII CATG 1 cut(s) 74
DdeI CTNAG 4 cut(s) 51, 144, 530, 582
DpnI GATC 1 cut(s) 78
DpnII GATC 1 cut(s) 76
Eam1104I CTCTTC 3 cut(s) 324, 371, 435
EarI CTCTTC 3 cut(s) 324, 371, 435
Ecl136II GAGCTC 1 cut(s) 381
Eco147I AGGCCT 1 cut(s) 65
Eco24I GRGCYC 1 cut(s) 383
Eco47I GGWCC 1 cut(s) 205
Eco53kI GAGCTC 1 cut(s) 381
Eco57I CTGAAG 1 cut(s) 416
EcoICRI GAGCTC 1 cut(s) 381
EcoRI GAATTC 1 cut(s) 404
EcoRII CCWGG 2 cut(s) 340, 441
EcoT38I GRGCYC 1 cut(s) 383
FaeI CATG 1 cut(s) 77
FaiI YATR 2 cut(s) 75, 481
FaqI GGGAC 1 cut(s) 470
FatI CATG 1 cut(s) 73
Fnu4HI GCNGC 8 cut(s) 225, 228, 231, 234, 237, 240, 469, 609
FokI GGATG 1 cut(s) 521
FriOI GRGCYC 1 cut(s) 383
Fsp4HI GCNGC 8 cut(s) 225, 228, 231, 234, 237, 240, 469, 609
FspBI CTAG 1 cut(s) 613
GluI GCNGC 8 cut(s) 225, 228, 231, 234, 237, 240, 469, 609
GsaI CCCAGC 1 cut(s) 361
GsuI CTGGAG 2 cut(s) 464, 477
HaeIII GGCC 2 cut(s) 65, 611
HapII CCGG 3 cut(s) 306, 503, 549
HgaI GACGC 1 cut(s) 166
Hin1II CATG 1 cut(s) 77
HinfI GANTC 1 cut(s) 475
HpaII CCGG 3 cut(s) 306, 503, 549
HphI GGTGA 2 cut(s) 35, 339
Hpy166II GTNNAC 3 cut(s) 212, 293, 347
Hpy188III TCNNGA 2 cut(s) 74, 584
Hpy8I GTNNAC 3 cut(s) 212, 293, 347
HpyCH4V TGCA 9 cut(s) 116, 188, 197, 233, 390, 397, 416, 518, 575
HpyF10VI GCNNNNNNNGC 6 cut(s) 194, 230, 233, 236, 239, 387
HpyF3I CTNAG 4 cut(s) 51, 144, 530, 582
Hsp92II CATG 1 cut(s) 77
Kzo9I GATC 1 cut(s) 76
LguI GCTCTTC 1 cut(s) 371
LmnI GCTCC 3 cut(s) 142, 343, 386
Lsp1109I GCAGC 6 cut(s) 214, 217, 223, 226, 245, 480
LweI GCATC 1 cut(s) 175
MaeI CTAG 1 cut(s) 613
MaeIII GTNAC 1 cut(s) 190
MalI GATC 1 cut(s) 78
MboI GATC 1 cut(s) 76
MboII GAAGA 8 cut(s) 56, 148, 311, 321, 388, 422, 432, 537
MfeI CAATTG 1 cut(s) 411
MhlI GDGCHC 2 cut(s) 139, 383
MluCI AATT 6 cut(s) 57, 325, 392, 404, 411, 591
MlyI GAGTC 1 cut(s) 484
MnlI CCTC 5 cut(s) 46, 55, 76, 294, 539
MroXI GAANNNNTTC 1 cut(s) 591
MseI TTAA 2 cut(s) 90, 120
MspA1I CMGCKG 2 cut(s) 236, 279
MspI CCGG 3 cut(s) 306, 503, 549
MspR9I CCNGG 3 cut(s) 307, 342, 443
MunI CAATTG 1 cut(s) 411
MvaI CCWGG 2 cut(s) 342, 443
MvnI CGCG 1 cut(s) 224
MwoI GCNNNNNNNGC 6 cut(s) 194, 230, 233, 236, 239, 387
NciI CCSGG 1 cut(s) 307
NdeII GATC 1 cut(s) 76
NlaIII CATG 1 cut(s) 77
PagI TCATGA 1 cut(s) 73
PceI AGGCCT 1 cut(s) 65
PciSI GCTCTTC 1 cut(s) 371
PdmI GAANNNNTTC 1 cut(s) 591
PfoI TCCNGGA 1 cut(s) 441
PinAI ACCGGT 1 cut(s) 502
PkrI GCNGC 8 cut(s) 226, 229, 232, 235, 238, 241, 470, 610
PleI GAGTC 1 cut(s) 483
PpsI GAGTC 1 cut(s) 483
PshBI ATTAAT 1 cut(s) 90
Psp124BI GAGCTC 1 cut(s) 383
Psp6I CCWGG 2 cut(s) 340, 441
PspFI CCCAGC 1 cut(s) 357
PspGI CCWGG 2 cut(s) 340, 441
PspPI GGNCC 1 cut(s) 205
PstI CTGCAG 3 cut(s) 199, 235, 577
PstNI CAGNNNCTG 2 cut(s) 194, 239
PvuII CAGCTG 2 cut(s) 236, 279
SacI GAGCTC 1 cut(s) 383
SapI GCTCTTC 1 cut(s) 371
SaqAI TTAA 2 cut(s) 90, 120
SatI GCNGC 8 cut(s) 225, 228, 231, 234, 237, 240, 469, 609
Sau3AI GATC 1 cut(s) 76
Sau96I GGNCC 1 cut(s) 205
SchI GAGTC 1 cut(s) 484
ScrFI CCNGG 3 cut(s) 307, 342, 443
SduI GDGCHC 2 cut(s) 139, 383
SfaNI GCATC 1 cut(s) 175
SfcI CTRYAG 3 cut(s) 195, 231, 573
SgrAI CRCCGGYG 1 cut(s) 502
SinI GGWCC 1 cut(s) 205
SmlI CTYRAG 1 cut(s) 14
SmoI CTYRAG 1 cut(s) 14
Sse9I AATT 6 cut(s) 57, 325, 392, 404, 411, 591
SseBI AGGCCT 1 cut(s) 65
SsiI CCGC 2 cut(s) 224, 608
SspMI CTAG 1 cut(s) 613
SstI GAGCTC 1 cut(s) 383
StuI AGGCCT 1 cut(s) 65
StyD4I CCNGG 3 cut(s) 305, 340, 441
TaqI TCGA 1 cut(s) 311
TasI AATT 6 cut(s) 57, 325, 392, 404, 411, 591
TauI GCSGC 2 cut(s) 227, 611
Tru1I TTAA 2 cut(s) 90, 120
Tru9I TTAA 2 cut(s) 90, 120
TscAI CASTG 1 cut(s) 582
TseI GCWGC 6 cut(s) 227, 230, 233, 236, 239, 468
TspGWI ACGGA 1 cut(s) 197
TspRI CASTG 1 cut(s) 582
VpaK11BI GGWCC 1 cut(s) 205
VspI ATTAAT 1 cut(s) 90
XapI RAATTY 3 cut(s) 325, 404, 591
XmnI GAANNNNTTC 1 cut(s) 591
XspI CTAG 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.