RLG00000020219

Non-lysosomal glucosylceramidase that catalyzes the conversion of glucosylceramide to free glucose and ceramide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010553
Physical Location & Seq
Forward (+)
62587321 .. 62589929
2609 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020219

Sequence Viewer

Length: 369 bp
ATGAATTCAAATTTGTGGAAGAATTCATGGGCGCCTCAGGAGTATATTCCCAGGAACACTTTACAATTGAGGAGGAAGTTCAACAGCCATGCTAATCTTCTTAAAGAATTTAGTATTACATTTAGGGAAGCAATAAAGGTGGGTAGGTTAGGTGTAAGCTTATGGTCGTACATATGTCAGAGAAGAGGCTTCTCATGGAAGGGTGTAGATCGATTGCAAACAACAACAACTCTTGCTTTGCAAAAGAAATTAAAGGACAAGATGGCTGAATTTCAGACTCTAAGAGAAACCATTCATCAAGAGTATTGGGAGGTTGTCGAGAGGTACCATTTCTCCTTTTTTCAAGACTCTGAGCAGAGATGCTACTGA

Protein Analysis

123

Amino Acids

14.83

Weight (kDa)

9.77

Isoelectric Point (pI)

40.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Syntaxin PF00804 68 - 108 7.3e-09 Syntaxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 324
AccB1I GGYRCC 2 cut(s) 31, 324
AcsI RAATTY 5 cut(s) 4, 10, 22, 107, 269
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 2 cut(s) 170, 326
AgsI TTSAA 3 cut(s) 9, 82, 344
AjnI CCWGG 1 cut(s) 50
AluBI AGCT 1 cut(s) 159
AluI AGCT 1 cut(s) 159
ApoI RAATTY 5 cut(s) 4, 10, 22, 107, 269
Asp700I GAANNNNTTC 1 cut(s) 291
Asp718I GGTACC 1 cut(s) 324
AspLEI GCGC 1 cut(s) 34
AxyI CCTNAGG 1 cut(s) 36
BanI GGYRCC 2 cut(s) 31, 324
BccI CCATC 1 cut(s) 256
BciT130I CCWGG 1 cut(s) 52
BfoI RGCGCY 1 cut(s) 35
Bme1390I CCNGG 1 cut(s) 52
BmiI GGNNCC 2 cut(s) 33, 326
BmrFI CCNGG 1 cut(s) 52
BmsI GCATC 1 cut(s) 350
Bsa29I ATCGAT 1 cut(s) 211
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 50
BsaXI ACNNNNNCTCC 2 cut(s) 317, 347
Bse21I CCTNAGG 1 cut(s) 36
BseBI CCWGG 1 cut(s) 52
BseCI ATCGAT 1 cut(s) 211
BseDI CCNNGG 1 cut(s) 50
BseMII CTCAG 2 cut(s) 50, 342
BseRI GAGGAG 1 cut(s) 85
BshNI GGYRCC 2 cut(s) 31, 324
BshVI ATCGAT 1 cut(s) 211
Bsp143I GATC 1 cut(s) 208
BspCNI CTCAG 2 cut(s) 49, 343
BspDI ATCGAT 1 cut(s) 211
BspLI GGNNCC 2 cut(s) 33, 326
BspT107I GGYRCC 2 cut(s) 31, 324
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 1 cut(s) 208
BssNI GRCGYC 1 cut(s) 32
Bst2UI CCWGG 1 cut(s) 52
Bst6I CTCTTC 1 cut(s) 178
BstACI GRCGYC 1 cut(s) 32
BstDEI CTNAG 3 cut(s) 36, 281, 351
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 1 cut(s) 211
BstMBI GATC 1 cut(s) 208
BstNI CCWGG 1 cut(s) 52
BstSCI CCNGG 1 cut(s) 50
Bsu15I ATCGAT 1 cut(s) 211
Bsu36I CCTNAGG 1 cut(s) 36
BsuTUI ATCGAT 1 cut(s) 211
CfoI GCGC 1 cut(s) 34
ClaI ATCGAT 1 cut(s) 211
Csp6I GTAC 2 cut(s) 169, 325
CspCI CAANNNNNGTGG 2 cut(s) 120, 155
CviAII CATG 3 cut(s) 27, 89, 195
CviJI RGCY 4 cut(s) 87, 159, 189, 266
CviKI_1 RGCY 4 cut(s) 87, 159, 189, 266
CviQI GTAC 2 cut(s) 169, 325
DdeI CTNAG 3 cut(s) 36, 281, 351
DinI GGCGCC 1 cut(s) 33
DpnI GATC 1 cut(s) 210
DpnII GATC 1 cut(s) 208
Eam1104I CTCTTC 1 cut(s) 178
EarI CTCTTC 1 cut(s) 178
Eco81I CCTNAGG 1 cut(s) 36
EcoRI GAATTC 2 cut(s) 4, 22
EcoRII CCWGG 1 cut(s) 50
EgeI GGCGCC 1 cut(s) 33
EheI GGCGCC 1 cut(s) 33
FaeI CATG 3 cut(s) 30, 92, 198
FaiI YATR 7 cut(s) 28, 45, 90, 163, 173, 175, 196
FatI CATG 3 cut(s) 26, 88, 194
FauNDI CATATG 1 cut(s) 173
GlaI GCGC 1 cut(s) 33
HaeII RGCGCY 1 cut(s) 35
HhaI GCGC 1 cut(s) 34
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 3 cut(s) 30, 92, 198
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HindIII AAGCTT 1 cut(s) 157
HinfI GANTC 2 cut(s) 277, 347
Hpy188I TCNGA 3 cut(s) 180, 276, 352
Hpy188III TCNNGA 4 cut(s) 38, 299, 319, 344
HpyAV CCTTC 1 cut(s) 193
HpyCH4V TGCA 2 cut(s) 217, 241
HpyF3I CTNAG 3 cut(s) 36, 281, 351
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 3 cut(s) 30, 92, 198
HspAI GCGC 1 cut(s) 32
KasI GGCGCC 1 cut(s) 31
KpnI GGTACC 1 cut(s) 328
Kzo9I GATC 1 cut(s) 208
LpnPI CCDG 3 cut(s) 23, 37, 64
LweI GCATC 1 cut(s) 350
MalI GATC 1 cut(s) 210
MboI GATC 1 cut(s) 208
MboII GAAGA 3 cut(s) 31, 89, 195
MfeI CAATTG 1 cut(s) 65
MluCI AATT 7 cut(s) 4, 10, 22, 65, 107, 248, 269
Mly113I GGCGCC 1 cut(s) 32
MlyI GAGTC 2 cut(s) 271, 341
MnlI CCTC 6 cut(s) 45, 63, 66, 179, 304, 315
MroXI GAANNNNTTC 1 cut(s) 291
MseI TTAA 2 cut(s) 102, 251
MspR9I CCNGG 1 cut(s) 52
MunI CAATTG 1 cut(s) 65
MvaI CCWGG 1 cut(s) 52
NarI GGCGCC 1 cut(s) 32
NdeI CATATG 1 cut(s) 173
NdeII GATC 1 cut(s) 208
NlaIII CATG 3 cut(s) 30, 92, 198
NlaIV GGNNCC 2 cut(s) 33, 326
PdmI GAANNNNTTC 1 cut(s) 291
PleI GAGTC 2 cut(s) 271, 341
PluTI GGCGCC 1 cut(s) 35
PpsI GAGTC 2 cut(s) 271, 341
Psp6I CCWGG 1 cut(s) 50
PspGI CCWGG 1 cut(s) 50
PspN4I GGNNCC 2 cut(s) 33, 326
RsaI GTAC 2 cut(s) 170, 326
RsaNI GTAC 2 cut(s) 169, 325
SaqAI TTAA 2 cut(s) 102, 251
Sau3AI GATC 1 cut(s) 208
SchI GAGTC 2 cut(s) 271, 341
ScrFI CCNGG 1 cut(s) 52
SetI ASST 6 cut(s) 141, 149, 154, 161, 315, 326
SfaNI GCATC 1 cut(s) 350
SfoI GGCGCC 1 cut(s) 33
Sse9I AATT 7 cut(s) 4, 10, 22, 65, 107, 248, 269
SspDI GGCGCC 1 cut(s) 31
StyD4I CCNGG 1 cut(s) 50
TaqI TCGA 2 cut(s) 211, 318
TasI AATT 7 cut(s) 4, 10, 22, 65, 107, 248, 269
Tru1I TTAA 2 cut(s) 102, 251
Tru9I TTAA 2 cut(s) 102, 251
TspDTI ATGAA 3 cut(s) 15, 17, 284
XapI RAATTY 5 cut(s) 4, 10, 22, 107, 269
XmnI GAANNNNTTC 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.