RLG00000020468

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005863
Physical Location & Seq
Forward (+)
65373202 .. 65378525
5324 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020468

Sequence Viewer

Length: 975 bp
ATGAAGGCGGCAGCAGCAGCAACCTTGAACCATCCCATTCGTCTCTACCCATCACCCATTTCCCGCCAAATCGACTTCCCGGCTCGACGGTCTCAACCTCTCGCACGCAACTCCGGCAAAGGGTTGAAATTGTACCGAAATGAGTCTGTGTGCTCCGTTTCTAGTGTAATTTGTGCTGTCAATGGAGGTCAGGGAGGGTATGTCTCTGCGTGGGACGAGAAACCATATGAGTTACTTCCGAATGGGAAAAGGGCTTACTTGGATGAACAAGATGTGGTCACGTTCCTTGATCCTCCTAAAGAGTTGATTCCTTTGGACTCAGCTTCTTATAATCCTGCTGCATATCTCTGGAAAAAAATTGATGATATTCCCCAGGAAAGGCACCGTCGGCTGCTGCGCTTGCTAGAGCCTAGGCTGATCTCAAGAGCTTGGGAGATAGCTGGCACACGATATGAAGAACCCAAGTTGATGAACAGAAGCTCATCTCAACTGTTGTCTAATGAAGATGGTGTCTCTCTTGAATATTATAATTGCCGAACAAGTGGAGGACCAATGCTCATTTCTTGGATAAACTTTTTCAAAACGGCAATTTTTTCTTGCAAGGATGGGAACGTATATGGAAGATTTATTGGTGAAACAATTCTTAATGGACTTGCAAATTCCTTTAGTCCACTGTACTTCGTAGTGAGCCAGCTGAAGGAAGTAATGGCAACTGAACAACCTTGTGATCTAGCATATGAGTTCAGGGATGGGCTGTTTGATCTCCATGAATACCCCGAGGGCTTTCCAAGACCAGTGAAACATCCGTATCCTTTCAACGATCAGGTGGTTGTCTATGTTCGTCATCTAGGACCAGGCGTGTCAGTAGGGCAAGCATGGCAAGAAGGAAAAGCATTACAACAAGTACCACAAAAATTATGTGGTGACATTCTAATGGTGAAAGATTATTCTGCTCTTAGAGAAGATCAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

325

Amino Acids

36.66

Weight (kDa)

6.96

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 330, 528
AccB1I GGYRCC 1 cut(s) 381
AciI CCGC 2 cut(s) 8, 64
AclWI GGATC 1 cut(s) 284
AcsI RAATTY 1 cut(s) 658
AcuI CTGAAG 1 cut(s) 716
AfaI GTAC 4 cut(s) 134, 677, 906, 971
AfiI CCNNNNNNNGG 3 cut(s) 120, 378, 697
AgsI TTSAA 5 cut(s) 28, 127, 521, 580, 817
AjnI CCWGG 2 cut(s) 372, 853
AjuI GAANNNNNNNTTGG 2 cut(s) 544, 576
AluBI AGCT 5 cut(s) 323, 428, 440, 480, 694
AluI AGCT 5 cut(s) 323, 428, 440, 480, 694
Alw21I GWGCWC 1 cut(s) 155
Alw26I GTCTC 4 cut(s) 47, 96, 208, 517
AlwI GGATC 1 cut(s) 284
Ama87I CYCGRG 1 cut(s) 776
ApeKI GCWGC 6 cut(s) 11, 14, 17, 338, 391, 394
ApoI RAATTY 1 cut(s) 658
Asp700I GAANNNNTTC 1 cut(s) 639
AspA2I CCTAGG 1 cut(s) 410
AspLEI GCGC 1 cut(s) 399
AspS9I GGNCC 2 cut(s) 548, 851
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 4 cut(s) 45, 644, 935, 949
AvaI CYCGRG 1 cut(s) 776
AvaII GGWCC 2 cut(s) 548, 851
AvrII CCTAGG 1 cut(s) 410
BaeI ACNNNNGTAYC 2 cut(s) 791, 824
BanI GGYRCC 1 cut(s) 381
Bbv12I GWGCWC 1 cut(s) 155
BbvI GCAGC 6 cut(s) 23, 26, 29, 325, 378, 381
BccI CCATC 5 cut(s) 39, 58, 500, 599, 743
BceAI ACGGC 1 cut(s) 600
BciT130I CCWGG 2 cut(s) 374, 855
BciVI GTATCC 1 cut(s) 819
BcnI CCSGG 1 cut(s) 80
BcoDI GTCTC 4 cut(s) 47, 96, 208, 517
BfaI CTAG 6 cut(s) 162, 404, 411, 731, 848, 973
BfuI GTATCC 1 cut(s) 819
BisI GCNGC 7 cut(s) 9, 12, 15, 18, 339, 392, 395
BlnI CCTAGG 1 cut(s) 410
BlsI GCNGC 7 cut(s) 10, 13, 16, 19, 340, 393, 396
BmcAI AGTACT 1 cut(s) 971
Bme1390I CCNGG 3 cut(s) 80, 374, 855
Bme18I GGWCC 2 cut(s) 548, 851
BmeT110I CYCGRG 1 cut(s) 776
BmgT120I GGNCC 2 cut(s) 548, 851
BmiI GGNNCC 1 cut(s) 383
BmrFI CCNGG 3 cut(s) 80, 374, 855
BpuEI CTTGAG 1 cut(s) 406
BpuMI CCSGG 1 cut(s) 80
BsaI GGTCTC 1 cut(s) 96
BsaJI CCNNGG 3 cut(s) 372, 410, 777
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 3 cut(s) 120, 378, 697
Bse1I ACTGG 1 cut(s) 794
BseBI CCWGG 2 cut(s) 374, 855
BseDI CCNNGG 3 cut(s) 372, 410, 777
BseGI GGATG 5 cut(s) 31, 268, 610, 754, 802
BseLI CCNNNNNNNGG 3 cut(s) 120, 378, 697
BseMII CTCAG 1 cut(s) 333
BseNI ACTGG 1 cut(s) 794
BseXI GCAGC 6 cut(s) 23, 26, 29, 325, 378, 381
BshNI GGYRCC 1 cut(s) 381
BsiHKAI GWGCWC 1 cut(s) 155
BsiHKCI CYCGRG 1 cut(s) 776
BsiSI CCGG 2 cut(s) 80, 114
BslFI GGGAC 1 cut(s) 227
BslI CCNNNNNNNGG 3 cut(s) 120, 378, 697
BsmAI GTCTC 4 cut(s) 47, 96, 208, 517
BsmBI CGTCTC 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 227
Bso31I GGTCTC 1 cut(s) 96
BsoBI CYCGRG 1 cut(s) 776
Bsp1286I GDGCHC 1 cut(s) 155
Bsp143I GATC 6 cut(s) 289, 417, 727, 760, 820, 964
BspACI CCGC 2 cut(s) 8, 64
BspCNI CTCAG 1 cut(s) 332
BspLI GGNNCC 1 cut(s) 383
BspPI GGATC 1 cut(s) 284
BspT107I GGYRCC 1 cut(s) 381
BspTNI GGTCTC 1 cut(s) 96
BsrI ACTGG 1 cut(s) 794
BssECI CCNNGG 3 cut(s) 372, 410, 777
BssMI GATC 6 cut(s) 289, 417, 727, 760, 820, 964
BssT1I CCWWGG 1 cut(s) 410
Bst2UI CCWGG 2 cut(s) 374, 855
Bst4CI ACNGT 4 cut(s) 90, 386, 492, 675
BstC8I GCNNGC 5 cut(s) 106, 401, 442, 692, 873
BstDEI CTNAG 2 cut(s) 319, 956
BstF5I GGATG 5 cut(s) 31, 268, 610, 754, 802
BstHHI GCGC 1 cut(s) 399
BstKTI GATC 6 cut(s) 292, 420, 730, 763, 823, 967
BstMAI GTCTC 4 cut(s) 47, 96, 208, 517
BstMBI GATC 6 cut(s) 289, 417, 727, 760, 820, 964
BstMWI GCNNNNNNNGC 6 cut(s) 14, 17, 114, 388, 400, 877
BstNI CCWGG 2 cut(s) 374, 855
BstSCI CCNGG 3 cut(s) 78, 372, 853
BstV1I GCAGC 6 cut(s) 23, 26, 29, 325, 378, 381
BsuI GTATCC 1 cut(s) 819
BtsCI GGATG 5 cut(s) 31, 268, 610, 754, 802
BtsIMutI CAGTG 2 cut(s) 671, 801
Cac8I GCNNGC 5 cut(s) 106, 401, 442, 692, 873
CfoI GCGC 1 cut(s) 399
Cfr13I GGNCC 2 cut(s) 548, 851
Csp6I GTAC 4 cut(s) 133, 676, 905, 970
CviAII CATG 2 cut(s) 767, 876
CviQI GTAC 4 cut(s) 133, 676, 905, 970
DdeI CTNAG 2 cut(s) 319, 956
DpnI GATC 6 cut(s) 291, 419, 729, 762, 822, 966
DpnII GATC 6 cut(s) 289, 417, 727, 760, 820, 964
Eco130I CCWWGG 1 cut(s) 410
Eco31I GGTCTC 1 cut(s) 96
Eco47I GGWCC 2 cut(s) 548, 851
Eco57I CTGAAG 1 cut(s) 716
Eco88I CYCGRG 1 cut(s) 776
EcoRII CCWGG 2 cut(s) 372, 853
EcoT14I CCWWGG 1 cut(s) 410
ErhI CCWWGG 1 cut(s) 410
Esp3I CGTCTC 1 cut(s) 47
FaeI CATG 2 cut(s) 770, 879
FaqI GGGAC 1 cut(s) 227
FatI CATG 2 cut(s) 766, 875
FauI CCCGC 1 cut(s) 71
FauNDI CATATG 2 cut(s) 226, 736
Fnu4HI GCNGC 7 cut(s) 9, 12, 15, 18, 339, 392, 395
FokI GGATG 5 cut(s) 18, 275, 617, 761, 789
Fsp4HI GCNGC 7 cut(s) 9, 12, 15, 18, 339, 392, 395
FspBI CTAG 6 cut(s) 162, 404, 411, 731, 848, 973
GlaI GCGC 1 cut(s) 398
GluI GCNGC 7 cut(s) 9, 12, 15, 18, 339, 392, 395
HapII CCGG 2 cut(s) 80, 114
HhaI GCGC 1 cut(s) 399
Hin1II CATG 2 cut(s) 770, 879
Hin6I GCGC 1 cut(s) 397
HinP1I GCGC 1 cut(s) 397
HinfI GANTC 3 cut(s) 143, 307, 317
HpaII CCGG 2 cut(s) 80, 114
HphI GGTGA 4 cut(s) 45, 644, 935, 949
Hpy166II GTNNAC 1 cut(s) 671
Hpy188I TCNGA 1 cut(s) 240
Hpy188III TCNNGA 3 cut(s) 349, 423, 518
Hpy8I GTNNAC 1 cut(s) 671
Hpy99I CGWCG 2 cut(s) 90, 390
HpyAV CCTTC 2 cut(s) 691, 878
HpyCH4III ACNGT 4 cut(s) 90, 386, 492, 675
HpyCH4IV ACGT 2 cut(s) 281, 612
HpyCH4V TGCA 3 cut(s) 341, 600, 656
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 17, 114, 388, 400, 877
HpyF3I CTNAG 2 cut(s) 319, 956
HpySE526I ACGT 2 cut(s) 281, 612
Hsp92II CATG 2 cut(s) 770, 879
HspAI GCGC 1 cut(s) 397
Kzo9I GATC 6 cut(s) 289, 417, 727, 760, 820, 964
LmnI GCTCC 1 cut(s) 158
Lsp1109I GCAGC 6 cut(s) 23, 26, 29, 325, 378, 381
MaeI CTAG 6 cut(s) 162, 404, 411, 731, 848, 973
MaeII ACGT 2 cut(s) 281, 612
MaeIII GTNAC 3 cut(s) 231, 277, 923
MalI GATC 6 cut(s) 291, 419, 729, 762, 822, 966
MboI GATC 6 cut(s) 289, 417, 727, 760, 820, 964
MboII GAAGA 4 cut(s) 467, 515, 633, 974
MhlI GDGCHC 1 cut(s) 155
MluCI AATT 8 cut(s) 128, 168, 357, 529, 588, 639, 658, 914
MlyI GAGTC 2 cut(s) 152, 311
MnlI CCTC 6 cut(s) 108, 179, 188, 303, 539, 772
MroXI GAANNNNTTC 1 cut(s) 639
MseI TTAA 1 cut(s) 645
MslI CAYNNNNRTG 1 cut(s) 932
MspA1I CMGCKG 1 cut(s) 694
MspI CCGG 2 cut(s) 80, 114
MspR9I CCNGG 3 cut(s) 80, 374, 855
MvaI CCWGG 2 cut(s) 374, 855
MwoI GCNNNNNNNGC 6 cut(s) 14, 17, 114, 388, 400, 877
NciI CCSGG 1 cut(s) 80
NdeI CATATG 2 cut(s) 226, 736
NdeII GATC 6 cut(s) 289, 417, 727, 760, 820, 964
NlaIII CATG 2 cut(s) 770, 879
NlaIV GGNNCC 1 cut(s) 383
NmuCI GTSAC 2 cut(s) 277, 923
PdmI GAANNNNTTC 1 cut(s) 639
PfeI GAWTC 1 cut(s) 307
PkrI GCNGC 7 cut(s) 10, 13, 16, 19, 340, 393, 396
PleI GAGTC 2 cut(s) 151, 311
PpsI GAGTC 2 cut(s) 151, 311
PsiI TTATAA 2 cut(s) 330, 528
Psp6I CCWGG 2 cut(s) 372, 853
PspGI CCWGG 2 cut(s) 372, 853
PspN4I GGNNCC 1 cut(s) 383
PspPI GGNCC 2 cut(s) 548, 851
PvuII CAGCTG 1 cut(s) 694
RsaI GTAC 4 cut(s) 134, 677, 906, 971
RsaNI GTAC 4 cut(s) 133, 676, 905, 970
RseI CAYNNNNRTG 1 cut(s) 932
SaqAI TTAA 1 cut(s) 645
SatI GCNGC 7 cut(s) 9, 12, 15, 18, 339, 392, 395
Sau3AI GATC 6 cut(s) 289, 417, 727, 760, 820, 964
Sau96I GGNCC 2 cut(s) 548, 851
ScaI AGTACT 1 cut(s) 971
SchI GAGTC 2 cut(s) 152, 311
ScrFI CCNGG 3 cut(s) 80, 374, 855
SduI GDGCHC 1 cut(s) 155
SinI GGWCC 2 cut(s) 548, 851
SmiMI CAYNNNNRTG 1 cut(s) 932
SmlI CTYRAG 1 cut(s) 421
SmoI CTYRAG 1 cut(s) 421
Sse9I AATT 8 cut(s) 128, 168, 357, 529, 588, 639, 658, 914
SsiI CCGC 2 cut(s) 8, 64
SspI AATATT 1 cut(s) 524
SspMI CTAG 6 cut(s) 162, 404, 411, 731, 848, 973
StyD4I CCNGG 3 cut(s) 78, 372, 853
StyI CCWWGG 1 cut(s) 410
TaaI ACNGT 4 cut(s) 90, 386, 492, 675
TaiI ACGT 2 cut(s) 284, 615
TaqI TCGA 2 cut(s) 72, 85
TasI AATT 8 cut(s) 128, 168, 357, 529, 588, 639, 658, 914
TatI WGTACW 2 cut(s) 675, 969
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 1 cut(s) 307
Tru1I TTAA 1 cut(s) 645
Tru9I TTAA 1 cut(s) 645
TscAI CASTG 2 cut(s) 678, 801
TseFI GTSAC 2 cut(s) 277, 923
TseI GCWGC 6 cut(s) 11, 14, 17, 338, 391, 394
Tsp45I GTSAC 2 cut(s) 277, 923
TspDTI ATGAA 6 cut(s) 17, 279, 468, 485, 516, 783
TspGWI ACGGA 2 cut(s) 145, 795
TspRI CASTG 2 cut(s) 678, 801
VpaK11BI GGWCC 2 cut(s) 548, 851
XapI RAATTY 1 cut(s) 658
XmaJI CCTAGG 1 cut(s) 410
XmnI GAANNNNTTC 1 cut(s) 639
XspI CTAG 6 cut(s) 162, 404, 411, 731, 848, 973
ZrmI AGTACT 1 cut(s) 971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.