RLG00000022502

Importin-5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0014648
Physical Location & Seq
Reverse (-)
2182602 .. 2184548
1947 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022502

Sequence Viewer

Length: 1251 bp
ATGGCGTCGTTCTCACCCTTTATCGTACCCGATTCGCCAGACCAAGTCAAAGATGCTTGTGACGAGAACGAAGCTAATTCCAAGTACGTTATCATGTCCAACAAGACCACCGAGCAGACAAAGAAGAAGAGGAAGAAGAAGAAGAAGAAGACAAGGCACGCAGAGGAGACGAAGCAAGTCGACATCAACGGACCTGAAAAAGAAAATCAGTCTGTTGATGAGGAACAAGTCCCGAAAAACAAGAGGGTGAAAGTAGCAGCTTCACCGGCTCCAGAAGAGAACAAGTCCAACACCAAATCGTCTTGTGCTGGAATAACATGGAGCAAAGACGATGAGATTGTTATTTTGGGCGGCCTGATCTCGTTTCAGGGAGACGAGAGAGAGTATAAAAGCAAGGAATTTTATGAATACATAAAGAGTCGCCTCAGCCAGGGAGATTTGAACCAAGTAAGGGACAAAGTAAAATCCCTTAAGAGAAAATATTCGGGGATGCAGAATCAGACGTTCTTAGAAACTTTCCAGAAGGGGCGTTACCAAGAGATGAAGGCGTTCAACTTGTCCAAAATTTATTGGGGTAAGTTTGATGCTGCTGATGATGACAAATTAAACAAGGAGAAGGAGGAGGAGAAGGATGAAACAATACTAGTGCAGGTGGCTGACGATGAGGAGGAGGTGGAGGAGGAAAAAGAGGAGGAAACAATACAAGTGAAGGTGGCGGACTATGAGAAGATGATTGATGATGATGATAAGAGCAAGCCAAAGATTAGTGGAGTTCTTCGTCGTGGTTTGGGAGACCATTTTCGGAGAGAGCACTTTGATCACAGGTGGGCTGAGGAGCAGGATCTCGAGGACTTGGAGAACAAGTGGAAGGCATACAAAATTGCTGAGGCCCAATCGAATTTGGCCGAAGCCCATTTTCGAAAAGCGTTCAACTGCTGCATACTCAAGGCCTACCGGATCTTTGAGCTCCAGATCTTCATTGTTGATAGACAACTCTCTTTCTTCCTCCTTTGGTTTCAAATTGAGCTCCCGATCTTCGTCGTCTACGGCTTCGACGCCATCAACGTCACAGAGCATGTGCCGGCGGCTCTCGGCGCGGCGGGATTGGCGGAGGTGGTGGCTGAGTCCTACGCGCACATCTTCTTCACGAACTCGGTGGCCACCGGCAAGGTGTACCCTCGACTGGAGTCATTGACTTCGCCGGCCCCTGCGTTGCCATCATCTGCAGCTTCGTCGCCGCTGTCGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HEAT HEAT_2 HEAT_EZ IBN_N

Protein Analysis

417

Amino Acids

47.62

Weight (kDa)

5.43

Isoelectric Point (pI)

50.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 106 - 192 3.5e-14 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 640
Acc36I ACCTGC 1 cut(s) 640
AccI GTMKAC 2 cut(s) 180, 1044
AccII CGCG 2 cut(s) 1097, 1133
AciI CCGC 7 cut(s) 351, 716, 1085, 1097, 1100, 1109, 1238
AclWI GGATC 2 cut(s) 849, 965
AcoI YGGCCR 2 cut(s) 903, 1158
AcsI RAATTY 3 cut(s) 398, 564, 898
AcyI GRCGYC 2 cut(s) 5, 1056
AfaI GTAC 3 cut(s) 27, 86, 1175
AfiI CCNNNNNNNGG 3 cut(s) 430, 451, 1183
AflII CTTAAG 1 cut(s) 470
AgsI TTSAA 4 cut(s) 442, 553, 931, 1019
AhlI ACTAGT 1 cut(s) 643
AjnI CCWGG 1 cut(s) 429
AluBI AGCT 5 cut(s) 74, 260, 967, 1027, 1229
AluI AGCT 5 cut(s) 74, 260, 967, 1027, 1229
Alw21I GWGCWC 3 cut(s) 813, 969, 1029
Alw26I GTCTC 3 cut(s) 161, 366, 786
AlwI GGATC 2 cut(s) 849, 965
Ama87I CYCGRG 1 cut(s) 845
AoxI GGCC 6 cut(s) 352, 888, 903, 948, 1158, 1203
ApeKI GCWGC 4 cut(s) 257, 587, 936, 1226
ApoI RAATTY 3 cut(s) 398, 564, 898
Asp700I GAANNNNTTC 2 cut(s) 481, 548
AspLEI GCGC 2 cut(s) 1097, 1135
AspS9I GGNCC 3 cut(s) 191, 889, 1204
AsuHPI GGTGA 3 cut(s) 6, 255, 259
AsuII TTCGAA 1 cut(s) 919
AvaI CYCGRG 1 cut(s) 845
AvaII GGWCC 1 cut(s) 191
BalI TGGCCA 1 cut(s) 1160
BanII GRGCYC 2 cut(s) 969, 1029
BarI GAAGNNNNNNTAC 2 cut(s) 515, 547
BbsI GAAGAC 1 cut(s) 155
Bbv12I GWGCWC 3 cut(s) 813, 969, 1029
BbvCI CCTCAGC 3 cut(s) 425, 831, 885
BbvI GCAGC 4 cut(s) 269, 574, 923, 1238
BccI CCATC 2 cut(s) 1067, 1225
BceAI ACGGC 1 cut(s) 1063
BcgI CGANNNNNNTGC 2 cut(s) 1215, 1249
BciT130I CCWGG 1 cut(s) 431
BclI TGATCA 1 cut(s) 817
BcoDI GTCTC 3 cut(s) 161, 366, 786
BcuI ACTAGT 1 cut(s) 643
BfaI CTAG 1 cut(s) 644
BfmI CTRYAG 1 cut(s) 1224
BfrI CTTAAG 1 cut(s) 470
BfuAI ACCTGC 1 cut(s) 640
BglII AGATCT 1 cut(s) 972
BisI GCNGC 8 cut(s) 258, 352, 588, 937, 1086, 1098, 1227, 1238
BlsI GCNGC 8 cut(s) 259, 353, 589, 938, 1087, 1099, 1228, 1239
Bme1390I CCNGG 1 cut(s) 431
Bme18I GGWCC 1 cut(s) 191
BmeT110I CYCGRG 1 cut(s) 845
BmgT120I GGNCC 3 cut(s) 191, 889, 1204
BmiI GGNNCC 2 cut(s) 270, 1206
BmrFI CCNGG 1 cut(s) 431
BmsI GCATC 3 cut(s) 43, 480, 574
BoxI GACNNNNGTC 1 cut(s) 1186
BpiI GAAGAC 1 cut(s) 155
BpmI CTGGAG 3 cut(s) 255, 953, 1205
Bpu10I CCTNAGC 3 cut(s) 425, 831, 885
Bpu14I TTCGAA 1 cut(s) 919
BpuEI CTTGAG 1 cut(s) 929
BsaHI GRCGYC 2 cut(s) 5, 1056
BsaI GGTCTC 1 cut(s) 786
BsaJI CCNNGG 1 cut(s) 430
BsaWI WCCGGW 1 cut(s) 954
BsaXI ACNNNNNCTCC 2 cut(s) 848, 878
Bsc4I CCNNNNNNNGG 3 cut(s) 430, 451, 1183
Bse118I RCCGGY 4 cut(s) 265, 1081, 1163, 1201
Bse1I ACTGG 1 cut(s) 1188
BseBI CCWGG 1 cut(s) 431
BseDI CCNNGG 1 cut(s) 430
BseGI GGATG 2 cut(s) 495, 637
BseLI CCNNNNNNNGG 3 cut(s) 430, 451, 1183
BseMII CTCAG 4 cut(s) 439, 822, 876, 1113
BseNI ACTGG 1 cut(s) 1188
BseRI GAGGAG 8 cut(s) 179, 635, 638, 680, 683, 692, 704, 848
BseXI GCAGC 4 cut(s) 269, 574, 923, 1238
BsgI GTGCAG 1 cut(s) 668
Bsh1236I CGCG 2 cut(s) 1097, 1133
BshFI GGCC 6 cut(s) 354, 890, 905, 950, 1160, 1205
BsiHKAI GWGCWC 3 cut(s) 813, 969, 1029
BsiHKCI CYCGRG 1 cut(s) 845
BsiSI CCGG 5 cut(s) 266, 955, 1082, 1164, 1202
BslFI GGGAC 2 cut(s) 215, 467
BslI CCNNNNNNNGG 3 cut(s) 430, 451, 1183
BsmAI GTCTC 3 cut(s) 161, 366, 786
BsmBI CGTCTC 2 cut(s) 161, 366
BsmFI GGGAC 2 cut(s) 215, 467
BsnI GGCC 6 cut(s) 354, 890, 905, 950, 1160, 1205
Bso31I GGTCTC 1 cut(s) 786
BsoBI CYCGRG 1 cut(s) 845
Bsp119I TTCGAA 1 cut(s) 919
Bsp1286I GDGCHC 3 cut(s) 813, 969, 1029
Bsp143I GATC 6 cut(s) 357, 817, 841, 957, 972, 1032
BspACI CCGC 7 cut(s) 351, 716, 1085, 1097, 1100, 1109, 1238
BspANI GGCC 6 cut(s) 354, 890, 905, 950, 1160, 1205
BspCNI CTCAG 4 cut(s) 438, 823, 877, 1114
BspFNI CGCG 2 cut(s) 1097, 1133
BspLI GGNNCC 2 cut(s) 270, 1206
BspMAI CTGCAG 1 cut(s) 1228
BspMI ACCTGC 1 cut(s) 640
BspPI GGATC 2 cut(s) 849, 965
BspT104I TTCGAA 1 cut(s) 919
BspTI CTTAAG 1 cut(s) 470
BspTNI GGTCTC 1 cut(s) 786
BsrFI RCCGGY 4 cut(s) 265, 1081, 1163, 1201
BsrI ACTGG 1 cut(s) 1188
BssAI RCCGGY 4 cut(s) 265, 1081, 1163, 1201
BssECI CCNNGG 1 cut(s) 430
BssMI GATC 6 cut(s) 357, 817, 841, 957, 972, 1032
BssNI GRCGYC 2 cut(s) 5, 1056
Bst2UI CCWGG 1 cut(s) 431
Bst6I CTCTTC 2 cut(s) 122, 270
BstACI GRCGYC 2 cut(s) 5, 1056
BstAFI CTTAAG 1 cut(s) 470
BstBI TTCGAA 1 cut(s) 919
BstC8I GCNNGC 4 cut(s) 159, 755, 1083, 1203
BstDEI CTNAG 6 cut(s) 425, 508, 831, 885, 1122, 1248
BstENI CCTNNNNNAGG 1 cut(s) 428
BstF5I GGATG 2 cut(s) 495, 637
BstFNI CGCG 2 cut(s) 1097, 1133
BstHHI GCGC 2 cut(s) 1097, 1135
BstKTI GATC 6 cut(s) 360, 820, 844, 960, 975, 1035
BstMAI GTCTC 3 cut(s) 161, 366, 786
BstMBI GATC 6 cut(s) 357, 817, 841, 957, 972, 1032
BstMWI GCNNNNNNNGC 3 cut(s) 266, 1094, 1106
BstNI CCWGG 1 cut(s) 431
BstNSI RCATGY 1 cut(s) 1079
BstPAI GACNNNNGTC 1 cut(s) 1186
BstSCI CCNGG 1 cut(s) 429
BstSFI CTRYAG 1 cut(s) 1224
BstUI CGCG 2 cut(s) 1097, 1133
BstV1I GCAGC 4 cut(s) 269, 574, 923, 1238
BstV2I GAAGAC 1 cut(s) 155
BstX2I RGATCY 3 cut(s) 841, 957, 972
BstYI RGATCY 3 cut(s) 841, 957, 972
BsuRI GGCC 6 cut(s) 354, 890, 905, 950, 1160, 1205
BtsCI GGATG 2 cut(s) 495, 637
BveI ACCTGC 1 cut(s) 640
Cac8I GCNNGC 4 cut(s) 159, 755, 1083, 1203
CfoI GCGC 2 cut(s) 1097, 1135
Cfr10I RCCGGY 4 cut(s) 265, 1081, 1163, 1201
Cfr13I GGNCC 3 cut(s) 191, 889, 1204
CseI GACGC 1 cut(s) 1064
Csp6I GTAC 3 cut(s) 26, 85, 1174
CviAII CATG 3 cut(s) 94, 318, 1076
CviQI GTAC 3 cut(s) 26, 85, 1174
DdeI CTNAG 6 cut(s) 425, 508, 831, 885, 1122, 1248
DpnI GATC 6 cut(s) 359, 819, 843, 959, 974, 1034
DpnII GATC 6 cut(s) 357, 817, 841, 957, 972, 1032
EaeI YGGCCR 2 cut(s) 903, 1158
Eam1104I CTCTTC 2 cut(s) 122, 270
EarI CTCTTC 2 cut(s) 122, 270
EciI GGCGGA 2 cut(s) 731, 1124
Ecl136II GAGCTC 2 cut(s) 967, 1027
Eco147I AGGCCT 1 cut(s) 950
Eco24I GRGCYC 2 cut(s) 969, 1029
Eco31I GGTCTC 1 cut(s) 786
Eco47I GGWCC 1 cut(s) 191
Eco53kI GAGCTC 2 cut(s) 967, 1027
Eco88I CYCGRG 1 cut(s) 845
EcoICRI GAGCTC 2 cut(s) 967, 1027
EcoNI CCTNNNNNAGG 1 cut(s) 428
EcoRII CCWGG 1 cut(s) 429
EcoT38I GRGCYC 2 cut(s) 969, 1029
Esp3I CGTCTC 2 cut(s) 161, 366
FaeI CATG 3 cut(s) 97, 321, 1079
FaiI YATR 9 cut(s) 95, 319, 387, 405, 413, 723, 874, 941, 1077
FaqI GGGAC 2 cut(s) 215, 467
FatI CATG 3 cut(s) 93, 317, 1075
FauI CCCGC 1 cut(s) 1093
FbaI TGATCA 1 cut(s) 817
FblI GTMKAC 2 cut(s) 180, 1044
Fnu4HI GCNGC 8 cut(s) 258, 352, 588, 937, 1086, 1098, 1227, 1238
FokI GGATG 2 cut(s) 502, 644
FriOI GRGCYC 2 cut(s) 969, 1029
Fsp4HI GCNGC 8 cut(s) 258, 352, 588, 937, 1086, 1098, 1227, 1238
FspBI CTAG 1 cut(s) 644
GlaI GCGC 2 cut(s) 1096, 1134
GluI GCNGC 8 cut(s) 258, 352, 588, 937, 1086, 1098, 1227, 1238
GsuI CTGGAG 3 cut(s) 255, 953, 1205
HaeIII GGCC 6 cut(s) 354, 890, 905, 950, 1160, 1205
HapII CCGG 5 cut(s) 266, 955, 1082, 1164, 1202
HgaI GACGC 1 cut(s) 1064
HhaI GCGC 2 cut(s) 1097, 1135
Hin1I GRCGYC 2 cut(s) 5, 1056
Hin1II CATG 3 cut(s) 97, 321, 1079
Hin6I GCGC 2 cut(s) 1095, 1133
HinP1I GCGC 2 cut(s) 1095, 1133
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 5 cut(s) 32, 418, 496, 1124, 1187
HpaII CCGG 5 cut(s) 266, 955, 1082, 1164, 1202
HphI GGTGA 3 cut(s) 6, 255, 259
Hpy166II GTNNAC 3 cut(s) 181, 1045, 1174
Hpy188I TCNGA 2 cut(s) 501, 804
Hpy188III TCNNGA 7 cut(s) 232, 272, 520, 845, 970, 1030, 1147
Hpy8I GTNNAC 3 cut(s) 181, 1045, 1174
Hpy99I CGWCG 5 cut(s) 10, 783, 1043, 1058, 1237
HpyAV CCTTC 6 cut(s) 517, 538, 610, 622, 703, 862
HpyCH4IV ACGT 3 cut(s) 87, 503, 1065
HpyCH4V TGCA 4 cut(s) 493, 649, 939, 1226
HpyF10VI GCNNNNNNNGC 3 cut(s) 266, 1094, 1106
HpyF3I CTNAG 6 cut(s) 425, 508, 831, 885, 1122, 1248
HpySE526I ACGT 3 cut(s) 87, 503, 1065
Hsp92I GRCGYC 2 cut(s) 5, 1056
Hsp92II CATG 3 cut(s) 97, 321, 1079
HspAI GCGC 2 cut(s) 1095, 1133
KroI GCCGGC 2 cut(s) 1081, 1201
KroNI GCCGGC 2 cut(s) 1083, 1203
Ksp22I TGATCA 1 cut(s) 817
Kzo9I GATC 6 cut(s) 357, 817, 841, 957, 972, 1032
LmnI GCTCC 5 cut(s) 274, 321, 835, 972, 1032
Lsp1109I GCAGC 4 cut(s) 269, 574, 923, 1238
LweI GCATC 3 cut(s) 43, 480, 574
MaeI CTAG 1 cut(s) 644
MaeII ACGT 3 cut(s) 87, 503, 1065
MaeIII GTNAC 3 cut(s) 59, 530, 1066
MalI GATC 6 cut(s) 359, 819, 843, 959, 974, 1034
MboI GATC 6 cut(s) 357, 817, 841, 957, 972, 1032
MflI RGATCY 3 cut(s) 841, 957, 972
MhlI GDGCHC 3 cut(s) 813, 969, 1029
MlsI TGGCCA 1 cut(s) 1160
MluCI AATT 7 cut(s) 76, 398, 564, 602, 879, 898, 1020
MluNI TGGCCA 1 cut(s) 1160
MlyI GAGTC 3 cut(s) 427, 1133, 1196
MmeI TCCRAC 2 cut(s) 123, 312
Mox20I TGGCCA 1 cut(s) 1160
MroNI GCCGGC 2 cut(s) 1081, 1201
MroXI GAANNNNTTC 2 cut(s) 481, 548
MscI TGGCCA 1 cut(s) 1160
MseI TTAA 2 cut(s) 471, 605
Msp20I TGGCCA 1 cut(s) 1160
MspA1I CMGCKG 1 cut(s) 1240
MspCI CTTAAG 1 cut(s) 470
MspI CCGG 5 cut(s) 266, 955, 1082, 1164, 1202
MspR9I CCNGG 1 cut(s) 431
MvaI CCWGG 1 cut(s) 431
MvnI CGCG 2 cut(s) 1097, 1133
MwoI GCNNNNNNNGC 3 cut(s) 266, 1094, 1106
NaeI GCCGGC 2 cut(s) 1083, 1203
NdeII GATC 6 cut(s) 357, 817, 841, 957, 972, 1032
NgoMIV GCCGGC 2 cut(s) 1081, 1201
NlaIII CATG 3 cut(s) 97, 321, 1079
NlaIV GGNNCC 2 cut(s) 270, 1206
NmeAIII GCCGAG 1 cut(s) 1071
NmuCI GTSAC 2 cut(s) 59, 1066
NspI RCATGY 1 cut(s) 1079
NspV TTCGAA 1 cut(s) 919
PaeR7I CTCGAG 1 cut(s) 845
PaqCI CACCTGC 1 cut(s) 640
PceI AGGCCT 1 cut(s) 950
PcsI WCGNNNNNNNCGW 2 cut(s) 1062, 1241
PdiI GCCGGC 2 cut(s) 1083, 1203
PdmI GAANNNNTTC 2 cut(s) 481, 548
PfeI GAWTC 2 cut(s) 32, 496
PflFI GACNNNGTC 1 cut(s) 44
PkrI GCNGC 8 cut(s) 259, 353, 589, 938, 1087, 1099, 1228, 1239
PleI GAGTC 3 cut(s) 426, 1132, 1195
PpsI GAGTC 3 cut(s) 426, 1132, 1195
PshAI GACNNNNGTC 1 cut(s) 1186
Psp124BI GAGCTC 2 cut(s) 969, 1029
Psp6I CCWGG 1 cut(s) 429
PspGI CCWGG 1 cut(s) 429
PspN4I GGNNCC 2 cut(s) 270, 1206
PspPI GGNCC 3 cut(s) 191, 889, 1204
PstI CTGCAG 1 cut(s) 1228
PsuI RGATCY 3 cut(s) 841, 957, 972
PsyI GACNNNGTC 1 cut(s) 44
RsaI GTAC 3 cut(s) 27, 86, 1175
RsaNI GTAC 3 cut(s) 26, 85, 1174
SacI GAGCTC 2 cut(s) 969, 1029
SalI GTCGAC 1 cut(s) 179
SaqAI TTAA 2 cut(s) 471, 605
SatI GCNGC 8 cut(s) 258, 352, 588, 937, 1086, 1098, 1227, 1238
Sau3AI GATC 6 cut(s) 357, 817, 841, 957, 972, 1032
Sau96I GGNCC 3 cut(s) 191, 889, 1204
SchI GAGTC 3 cut(s) 427, 1133, 1196
ScrFI CCNGG 1 cut(s) 431
SduI GDGCHC 3 cut(s) 813, 969, 1029
SfaNI GCATC 3 cut(s) 43, 480, 574
SfcI CTRYAG 1 cut(s) 1224
Sfr274I CTCGAG 1 cut(s) 845
SfuI TTCGAA 1 cut(s) 919
SinI GGWCC 1 cut(s) 191
SlaI CTCGAG 1 cut(s) 845
SmlI CTYRAG 3 cut(s) 470, 845, 944
SmoI CTYRAG 3 cut(s) 470, 845, 944
SpeI ACTAGT 1 cut(s) 643
Sse9I AATT 7 cut(s) 76, 398, 564, 602, 879, 898, 1020
SseBI AGGCCT 1 cut(s) 950
SsiI CCGC 7 cut(s) 351, 716, 1085, 1097, 1100, 1109, 1238
SspI AATATT 1 cut(s) 482
SspMI CTAG 1 cut(s) 644
SstI GAGCTC 2 cut(s) 969, 1029
StuI AGGCCT 1 cut(s) 950
StyD4I CCNGG 1 cut(s) 429
TaiI ACGT 3 cut(s) 90, 506, 1068
TaqI TCGA 6 cut(s) 180, 846, 896, 919, 1053, 1180
TasI AATT 7 cut(s) 76, 398, 564, 602, 879, 898, 1020
TauI GCSGC 4 cut(s) 354, 1088, 1100, 1240
TfiI GAWTC 2 cut(s) 32, 496
Tru1I TTAA 2 cut(s) 471, 605
Tru9I TTAA 2 cut(s) 471, 605
TseFI GTSAC 2 cut(s) 59, 1066
TseI GCWGC 4 cut(s) 257, 587, 936, 1226
Tsp45I GTSAC 2 cut(s) 59, 1066
TspDTI ATGAA 4 cut(s) 420, 557, 648, 967
TspGWI ACGGA 1 cut(s) 204
Tth111I GACNNNGTC 1 cut(s) 44
Vha464I CTTAAG 1 cut(s) 470
VpaK11BI GGWCC 1 cut(s) 191
XagI CCTNNNNNAGG 1 cut(s) 428
XapI RAATTY 3 cut(s) 398, 564, 898
XceI RCATGY 1 cut(s) 1079
XhoI CTCGAG 1 cut(s) 845
XmiI GTMKAC 2 cut(s) 180, 1044
XmnI GAANNNNTTC 2 cut(s) 481, 548
XspI CTAG 1 cut(s) 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.