Rorug03G0287300

Importin-5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0014648
Physical Location & Seq
Forward (+)
29130836 .. 29131324
489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0287300.1

Sequence Viewer

Length: 489 bp
ATGGAAACTATTGACAGTGCACCATTTGCGGAAGTAGTTGATTTTAAAGAGTCCAAGCCATATGGAACAAAGCAATATACAATCAAGGTTGATTGTTGGCGAAACAGGTTCAGTGAACGTGGCAACGAACCATACAAAACTTTGCCTGGGGATCTTTTTATTTTAGCAAATGCTAAACCTGAAACTGTTTCTGAATTACAACTGACTGGGAGGTCATGGTCTTTTGTATCTGTCACTCATCTTGTGTCTAACATCTCACAAATTCAGAAAAAAGATGAGAATGGGGATGAGGTTGAGGATGTGGAAGAGGAAACTTCTCTTTGTTTTAAAATCGAGTCTTCAAAAGAGTTTGAAGTCGATGATAGCAGATCACTGTTTGTTGTTTTCTTGTTGAATTTAATCCCCAGTGGAAGAATATACAAAGCATTGCACATGCTGGGAAATTTAACAATTATCAAAGAAGTTCTATGCTCTGATATTCTGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HEAT HEAT_2 HEAT_EZ IBN_N

Protein Analysis

162

Amino Acids

18.52

Weight (kDa)

4.77

Isoelectric Point (pI)

39.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 8 - 149 6.9e-51 Domain of unknown function (DUF6469)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 211
AciI CCGC 1 cut(s) 29
AclWI GGATC 1 cut(s) 159
AcsI RAATTY 3 cut(s) 261, 394, 442
AgsI TTSAA 3 cut(s) 342, 353, 394
AjnI CCWGG 1 cut(s) 145
Alw21I GWGCWC 1 cut(s) 22
Alw44I GTGCAC 1 cut(s) 18
AlwI GGATC 1 cut(s) 159
ApaLI GTGCAC 1 cut(s) 18
ApoI RAATTY 3 cut(s) 261, 394, 442
BaeGI GKGCMC 1 cut(s) 22
BbsI GAAGAC 1 cut(s) 330
Bbv12I GWGCWC 1 cut(s) 22
BciT130I CCWGG 1 cut(s) 147
Bme1390I CCNGG 1 cut(s) 147
BmrFI CCNGG 1 cut(s) 147
BmrI ACTGGG 2 cut(s) 216, 399
BmuI ACTGGG 2 cut(s) 216, 399
BpiI GAAGAC 1 cut(s) 330
BsaJI CCNNGG 1 cut(s) 146
Bse1I ACTGG 2 cut(s) 211, 405
Bse3DI GCAATG 1 cut(s) 425
BseBI CCWGG 1 cut(s) 147
BseDI CCNNGG 1 cut(s) 146
BseGI GGATG 2 cut(s) 292, 304
BseMI GCAATG 1 cut(s) 425
BseNI ACTGG 2 cut(s) 211, 405
BseSI GKGCMC 1 cut(s) 22
BseYI CCCAGC 1 cut(s) 436
BsiHKAI GWGCWC 1 cut(s) 22
Bsp1286I GDGCHC 1 cut(s) 22
Bsp143I GATC 2 cut(s) 151, 368
BspACI CCGC 1 cut(s) 29
BspPI GGATC 1 cut(s) 159
BsrDI GCAATG 1 cut(s) 425
BsrI ACTGG 2 cut(s) 211, 405
BssECI CCNNGG 1 cut(s) 146
BssMI GATC 2 cut(s) 151, 368
Bst2UI CCWGG 1 cut(s) 147
Bst4CI ACNGT 3 cut(s) 17, 187, 375
Bst6I CTCTTC 1 cut(s) 300
BstAPI GCANNNNNTGC 1 cut(s) 26
BstF5I GGATG 2 cut(s) 292, 304
BstKTI GATC 2 cut(s) 154, 371
BstMBI GATC 2 cut(s) 151, 368
BstMWI GCNNNNNNNGC 1 cut(s) 26
BstNI CCWGG 1 cut(s) 147
BstNSI RCATGY 1 cut(s) 436
BstSCI CCNGG 1 cut(s) 145
BstSLI GKGCMC 1 cut(s) 22
BstV2I GAAGAC 1 cut(s) 330
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BtsCI GGATG 2 cut(s) 292, 304
BtsIMutI CAGTG 4 cut(s) 22, 118, 371, 412
CviAII CATG 2 cut(s) 216, 433
CviJI RGCY 1 cut(s) 58
CviKI_1 RGCY 1 cut(s) 58
DpnI GATC 2 cut(s) 153, 370
DpnII GATC 2 cut(s) 151, 368
DraI TTTAAA 2 cut(s) 46, 328
DrdI GACNNNNNNGTC 1 cut(s) 211
DseDI GACNNNNNNGTC 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
EcoRII CCWGG 1 cut(s) 145
FaeI CATG 2 cut(s) 219, 436
FaiI YATR 8 cut(s) 61, 63, 78, 133, 217, 418, 434, 469
FatI CATG 2 cut(s) 215, 432
FauNDI CATATG 1 cut(s) 61
FokI GGATG 2 cut(s) 299, 311
GsaI CCCAGC 1 cut(s) 440
Hin1II CATG 2 cut(s) 219, 436
HinfI GANTC 2 cut(s) 50, 335
Hpy166II GTNNAC 2 cut(s) 20, 116
Hpy188I TCNGA 3 cut(s) 193, 267, 475
Hpy8I GTNNAC 2 cut(s) 20, 116
HpyCH4III ACNGT 3 cut(s) 17, 187, 375
HpyCH4IV ACGT 1 cut(s) 118
HpyCH4V TGCA 2 cut(s) 20, 430
HpyF10VI GCNNNNNNNGC 1 cut(s) 26
HpySE526I ACGT 1 cut(s) 118
Hsp92II CATG 2 cut(s) 219, 436
Kzo9I GATC 2 cut(s) 151, 368
LpnPI CCDG 7 cut(s) 91, 132, 159, 192, 192, 418, 422
MaeII ACGT 1 cut(s) 118
MaeIII GTNAC 1 cut(s) 232
MalI GATC 2 cut(s) 153, 370
MboI GATC 2 cut(s) 151, 368
MboII GAAGA 3 cut(s) 317, 330, 423
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 1 cut(s) 22
MluCI AATT 5 cut(s) 194, 261, 394, 442, 450
MlyI GAGTC 2 cut(s) 59, 344
MnlI CCTC 4 cut(s) 204, 283, 289, 301
MseI TTAA 4 cut(s) 45, 327, 398, 446
MspR9I CCNGG 1 cut(s) 147
MvaI CCWGG 1 cut(s) 147
MwoI GCNNNNNNNGC 1 cut(s) 26
NdeI CATATG 1 cut(s) 61
NdeII GATC 2 cut(s) 151, 368
NlaIII CATG 2 cut(s) 219, 436
NmuCI GTSAC 1 cut(s) 232
NspI RCATGY 1 cut(s) 436
PleI GAGTC 2 cut(s) 58, 343
PpsI GAGTC 2 cut(s) 58, 343
Psp6I CCWGG 1 cut(s) 145
PspFI CCCAGC 1 cut(s) 436
PspGI CCWGG 1 cut(s) 145
PsuI RGATCY 1 cut(s) 151
SaqAI TTAA 4 cut(s) 45, 327, 398, 446
Sau3AI GATC 2 cut(s) 151, 368
SchI GAGTC 2 cut(s) 59, 344
ScrFI CCNGG 1 cut(s) 147
SduI GDGCHC 1 cut(s) 22
SetI ASST 6 cut(s) 90, 110, 121, 181, 215, 294
Sse9I AATT 5 cut(s) 194, 261, 394, 442, 450
SsiI CCGC 1 cut(s) 29
StyD4I CCNGG 1 cut(s) 145
TaaI ACNGT 3 cut(s) 17, 187, 375
TaiI ACGT 1 cut(s) 121
TaqI TCGA 2 cut(s) 333, 357
TasI AATT 5 cut(s) 194, 261, 394, 442, 450
Tru1I TTAA 4 cut(s) 45, 327, 398, 446
Tru9I TTAA 4 cut(s) 45, 327, 398, 446
TscAI CASTG 4 cut(s) 22, 118, 378, 412
TseFI GTSAC 1 cut(s) 232
Tsp45I GTSAC 1 cut(s) 232
TspRI CASTG 4 cut(s) 22, 118, 378, 412
VneI GTGCAC 1 cut(s) 18
XapI RAATTY 3 cut(s) 261, 394, 442
XceI RCATGY 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.