RLG00000024837

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0008870
Physical Location & Seq
Reverse (-)
39513375 .. 39514739
1365 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024837

Sequence Viewer

Length: 1365 bp
ATGGATATCAACAATAAACTCGACGGCATCCCCGTGTTGCGTCTCAACACAGCAGAGAAAGAGATAAGAGTGGAGACTCGCATCTGTAGAGTTCCGGAGAAACTTCGAGAACTGAAGGAGGACGCCTACAATCCCCGACTTGTTTCAATTGGACCTTTCCATTCCTGTAAGGATTACCTTGCACCAATGTCACGACATAAATACTATTACATGTCCCTTCTCATTCACAGCAAAGATGCTGAAAACTGCTTGGATGAATGCATCTGTGCTATTATTGATTTTGACGATGTGATTCGTCAATGCTACGTCGAAGATATCAGAGTTTCAAATGATGAGCTTAGACTAATGATGTTAGTTGATGGCTGCTTCATATTGGAACTTCTTCTCAGGTTAGCACCTAACTTGGAGTATATAATGCCTCAAGAATACGAATCTGATCCATTAATAAACAATGCTTGGATGATCAGAGCTCTTTGGCACGATCTAGCATTGCTTGAAAACCAGATTCCCTTCTTTATTCTGGAAAAGTTATACGATATTATCAAGCCTCGTTTAATGAGCAAGTGCAGAGCACCAGACTCGGTCACTAGTCTTGCTTTAAGTTTCTTTAAGCCTATGATGAGCCAAAACTCAAATATAGAGGAGCATTCGTACCCGGACTGCAAACATTTGCTTGATCTCTTGCACAAATGCCCCCTGCTCGCAGCTTCTGGAGTAGATACTGCCTTCACAGCCTACGACGTCAGAGATGGGAGATCTAGAATTGCTTTAAACCATGCTGCAGCCGCTAATTTGACTCCAGGGAATCAATATTGGGGATTGAAGTATTGCGCTGAAGAACTTTTACGGTCCGGAATTGAGCTTTTTAAAGAGTCAGAGGCTGACTCTTTGGTGAACATAACCACCACGAGACACGGTCAGCTGATCAAAATCCCACCGCTGTTCATTGATGAGGTAAGGGATTCACTCTTCAGGAACCTGATTGCCTACGAGCAAAGCAGTATTAAAATTAGTCATCATGTTACATCTTATGTTATCCTCATGAAAAGTCTTATACGTTCTCCACGAGATGTCAAGCTTCTTAAGGAAAAAGGCATCATATCTCAGTATTGGATTGACGACCAAGAATATTCAACTTACTTCAGAAGTCTTCTAGATGAAGTTTTTCCCACGGACTTCTATTTCGGTTCTCTGTGTGGCCAAGTGGATGCCTACGCGAATAAGTTCTGGTTTCGTAGAAAGATGAGCGCTCTTTATCACACTTATTTCTCAACAGCATGGAGTATGACATCATTCATTGCTGCCATTTGCCTTTTCATTCTCACGGTCTTACAAACATACTTTACCATCAGCCCAGCTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

455

Amino Acids

52.63

Weight (kDa)

5.88

Isoelectric Point (pI)

48.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 28 - 441 1.4e-100 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 744
AccII CGCG 1 cut(s) 1217
AccIII TCCGGA 2 cut(s) 94, 851
AciI CCGC 2 cut(s) 786, 938
AclWI GGATC 1 cut(s) 431
AcoI YGGCCR 1 cut(s) 1198
AcuI CTGAAG 4 cut(s) 134, 855, 955, 1126
AcyI GRCGYC 2 cut(s) 123, 741
AfaI GTAC 1 cut(s) 653
AfeI AGCGCT 1 cut(s) 1249
AflII CTTAAG 1 cut(s) 1082
AflIII ACRYGT 1 cut(s) 210
AgsI TTSAA 5 cut(s) 147, 327, 497, 823, 1134
AhlI ACTAGT 1 cut(s) 587
AjnI CCWGG 1 cut(s) 799
AluBI AGCT 7 cut(s) 337, 470, 707, 862, 922, 1078, 1358
AluI AGCT 7 cut(s) 337, 470, 707, 862, 922, 1078, 1358
Alw21I GWGCWC 2 cut(s) 472, 574
Alw26I GTCTC 3 cut(s) 47, 68, 904
AlwI GGATC 1 cut(s) 431
AlwNI CAGNNNCTG 2 cut(s) 710, 881
Aor13HI TCCGGA 2 cut(s) 94, 851
Aor51HI AGCGCT 1 cut(s) 1249
AoxI GGCC 1 cut(s) 1198
ApeKI GCWGC 5 cut(s) 363, 704, 779, 782, 1301
ArsI GACNNNNNNTTYG 2 cut(s) 1166, 1198
AseI ATTAAT 1 cut(s) 443
Asp700I GAANNNNTTC 3 cut(s) 381, 1164, 1223
AspLEI GCGC 2 cut(s) 833, 1250
AspS9I GGNCC 2 cut(s) 152, 849
AsuC2I CCSGG 1 cut(s) 656
AsuHPI GGTGA 1 cut(s) 904
AvaII GGWCC 2 cut(s) 152, 849
BalI TGGCCA 1 cut(s) 1200
BanII GRGCYC 1 cut(s) 472
BarI GAAGNNNNNNTAC 2 cut(s) 828, 860
BauI CACGAG 2 cut(s) 907, 1065
BbsI GAAGAC 1 cut(s) 1142
Bbv12I GWGCWC 2 cut(s) 472, 574
BbvI GCAGC 5 cut(s) 350, 716, 766, 794, 1288
BccI CCATC 3 cut(s) 353, 743, 1355
BceAI ACGGC 1 cut(s) 40
BcgI CGANNNNNNTGC 2 cut(s) 561, 595
BciT130I CCWGG 1 cut(s) 801
BclI TGATCA 2 cut(s) 462, 924
BcnI CCSGG 1 cut(s) 656
BcoDI GTCTC 3 cut(s) 47, 68, 904
BcuI ACTAGT 1 cut(s) 587
BfaI CTAG 4 cut(s) 485, 588, 759, 1154
BfmI CTRYAG 2 cut(s) 85, 780
BfoI RGCGCY 1 cut(s) 1251
BfrI CTTAAG 1 cut(s) 1082
BglII AGATCT 1 cut(s) 755
BisI GCNGC 6 cut(s) 364, 705, 780, 783, 786, 1302
BlsI GCNGC 6 cut(s) 365, 706, 781, 784, 787, 1303
Bme1390I CCNGG 2 cut(s) 656, 801
Bme18I GGWCC 2 cut(s) 152, 849
BmgT120I GGNCC 2 cut(s) 152, 849
BmiI GGNNCC 1 cut(s) 977
BmrFI CCNGG 2 cut(s) 656, 801
BmsI GCATC 6 cut(s) 36, 90, 226, 270, 1104, 1198
BpiI GAAGAC 1 cut(s) 1142
BplI GAGNNNNNCTC 2 cut(s) 869, 901
BpmI CTGGAG 2 cut(s) 732, 783
BpuEI CTTGAG 1 cut(s) 405
BpuMI CCSGG 1 cut(s) 656
BsaBI GATNNNNATC 1 cut(s) 929
BsaHI GRCGYC 2 cut(s) 123, 741
BsaJI CCNNGG 2 cut(s) 800, 1170
BsaWI WCCGGW 2 cut(s) 94, 851
BsaXI ACNNNNNCTCC 2 cut(s) 635, 665
Bse3DI GCAATG 2 cut(s) 488, 1296
Bse8I GATNNNNATC 1 cut(s) 929
BseAI TCCGGA 2 cut(s) 94, 851
BseBI CCWGG 1 cut(s) 801
BseDI CCNNGG 2 cut(s) 800, 1170
BseGI GGATG 4 cut(s) 27, 259, 465, 1213
BseJI GATNNNNATC 1 cut(s) 929
BseMI GCAATG 2 cut(s) 488, 1296
BseMII CTCAG 2 cut(s) 400, 1118
BseRI GAGGAG 1 cut(s) 656
BseXI GCAGC 5 cut(s) 350, 716, 766, 794, 1288
BseYI CCCAGC 1 cut(s) 1354
BsgI GTGCAG 1 cut(s) 586
Bsh1236I CGCG 1 cut(s) 1217
BshFI GGCC 1 cut(s) 1200
BsiHKAI GWGCWC 2 cut(s) 472, 574
BsiSI CCGG 3 cut(s) 95, 656, 852
BslFI GGGAC 1 cut(s) 199
BsmAI GTCTC 3 cut(s) 47, 68, 904
BsmBI CGTCTC 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 199
BsmI GAATGC 2 cut(s) 263, 646
BsnI GGCC 1 cut(s) 1200
Bsp1286I GDGCHC 2 cut(s) 472, 574
Bsp13I TCCGGA 2 cut(s) 94, 851
Bsp143I GATC 6 cut(s) 436, 462, 481, 676, 755, 924
BspACI CCGC 2 cut(s) 786, 938
BspANI GGCC 1 cut(s) 1200
BspCNI CTCAG 2 cut(s) 399, 1117
BspEI TCCGGA 2 cut(s) 94, 851
BspFNI CGCG 1 cut(s) 1217
BspHI TCATGA 1 cut(s) 1041
BspLI GGNNCC 1 cut(s) 977
BspMAI CTGCAG 1 cut(s) 784
BspPI GGATC 1 cut(s) 431
BspTI CTTAAG 1 cut(s) 1082
BsrDI GCAATG 2 cut(s) 488, 1296
BssECI CCNNGG 2 cut(s) 800, 1170
BssMI GATC 6 cut(s) 436, 462, 481, 676, 755, 924
BssNI GRCGYC 2 cut(s) 123, 741
BssSI CACGAG 2 cut(s) 907, 1065
Bst2BI CACGAG 2 cut(s) 907, 1065
Bst2UI CCWGG 1 cut(s) 801
Bst4CI ACNGT 3 cut(s) 849, 917, 1327
Bst6I CTCTTC 1 cut(s) 974
BstACI GRCGYC 2 cut(s) 123, 741
BstAFI CTTAAG 1 cut(s) 1082
BstC8I GCNNGC 2 cut(s) 702, 1360
BstDEI CTNAG 3 cut(s) 338, 386, 1104
BstDSI CCRYGG 1 cut(s) 1170
BstF5I GGATG 4 cut(s) 27, 259, 465, 1213
BstFNI CGCG 1 cut(s) 1217
BstH2I RGCGCY 1 cut(s) 1251
BstHHI GCGC 2 cut(s) 833, 1250
BstKTI GATC 6 cut(s) 439, 465, 484, 679, 758, 927
BstMAI GTCTC 3 cut(s) 47, 68, 904
BstMBI GATC 6 cut(s) 436, 462, 481, 676, 755, 924
BstMWI GCNNNNNNNGC 3 cut(s) 731, 785, 1359
BstNI CCWGG 1 cut(s) 801
BstNSI RCATGY 1 cut(s) 214
BstSCI CCNGG 2 cut(s) 654, 799
BstSFI CTRYAG 2 cut(s) 85, 780
BstUI CGCG 1 cut(s) 1217
BstV1I GCAGC 5 cut(s) 350, 716, 766, 794, 1288
BstV2I GAAGAC 1 cut(s) 1142
BstX2I RGATCY 1 cut(s) 755
BstYI RGATCY 1 cut(s) 755
BsuRI GGCC 1 cut(s) 1200
BtgI CCRYGG 1 cut(s) 1170
BtsCI GGATG 4 cut(s) 27, 259, 465, 1213
Cac8I GCNNGC 2 cut(s) 702, 1360
CaiI CAGNNNCTG 2 cut(s) 710, 881
CciI TCATGA 1 cut(s) 1041
CfoI GCGC 2 cut(s) 833, 1250
Cfr13I GGNCC 2 cut(s) 152, 849
CpoI CGGWCCG 1 cut(s) 849
CseI GACGC 2 cut(s) 29, 131
Csp6I GTAC 1 cut(s) 652
CspI CGGWCCG 1 cut(s) 849
CviAII CATG 5 cut(s) 211, 776, 1019, 1042, 1278
CviQI GTAC 1 cut(s) 652
DdeI CTNAG 3 cut(s) 338, 386, 1104
DpnI GATC 6 cut(s) 438, 464, 483, 678, 757, 926
DpnII GATC 6 cut(s) 436, 462, 481, 676, 755, 924
DraI TTTAAA 2 cut(s) 771, 868
EaeI YGGCCR 1 cut(s) 1198
Eam1104I CTCTTC 1 cut(s) 974
EarI CTCTTC 1 cut(s) 974
Ecl136II GAGCTC 1 cut(s) 470
Eco24I GRGCYC 1 cut(s) 472
Eco32I GATATC 2 cut(s) 7, 316
Eco47I GGWCC 2 cut(s) 152, 849
Eco47III AGCGCT 1 cut(s) 1249
Eco53kI GAGCTC 1 cut(s) 470
Eco57I CTGAAG 4 cut(s) 134, 855, 955, 1126
EcoICRI GAGCTC 1 cut(s) 470
EcoRII CCWGG 1 cut(s) 799
EcoRV GATATC 2 cut(s) 7, 316
EcoT22I ATGCAT 1 cut(s) 263
EcoT38I GRGCYC 1 cut(s) 472
Esp3I CGTCTC 1 cut(s) 47
FaeI CATG 5 cut(s) 214, 779, 1022, 1045, 1281
FaqI GGGAC 1 cut(s) 199
FatI CATG 5 cut(s) 210, 775, 1018, 1041, 1277
FbaI TGATCA 2 cut(s) 462, 924
Fnu4HI GCNGC 6 cut(s) 364, 705, 780, 783, 786, 1302
FokI GGATG 4 cut(s) 14, 266, 472, 1220
FriOI GRGCYC 1 cut(s) 472
Fsp4HI GCNGC 6 cut(s) 364, 705, 780, 783, 786, 1302
FspBI CTAG 4 cut(s) 485, 588, 759, 1154
GlaI GCGC 2 cut(s) 832, 1249
GluI GCNGC 6 cut(s) 364, 705, 780, 783, 786, 1302
GsaI CCCAGC 1 cut(s) 1358
GsuI CTGGAG 2 cut(s) 732, 783
HaeII RGCGCY 1 cut(s) 1251
HaeIII GGCC 1 cut(s) 1200
HapII CCGG 3 cut(s) 95, 656, 852
HgaI GACGC 2 cut(s) 29, 131
HhaI GCGC 2 cut(s) 833, 1250
Hin1I GRCGYC 2 cut(s) 123, 741
Hin1II CATG 5 cut(s) 214, 779, 1022, 1045, 1281
Hin6I GCGC 2 cut(s) 831, 1248
HinP1I GCGC 2 cut(s) 831, 1248
HindIII AAGCTT 1 cut(s) 1076
HpaII CCGG 3 cut(s) 95, 656, 852
HphI GGTGA 1 cut(s) 904
Hpy166II GTNNAC 1 cut(s) 895
Hpy188I TCNGA 6 cut(s) 320, 436, 467, 746, 877, 1145
Hpy8I GTNNAC 1 cut(s) 895
Hpy99I CGWCG 3 cut(s) 26, 311, 743
HpyAV CCTTC 4 cut(s) 109, 227, 520, 736
HpyCH4III ACNGT 3 cut(s) 849, 917, 1327
HpyCH4IV ACGT 3 cut(s) 306, 741, 1057
HpyCH4V TGCA 6 cut(s) 182, 261, 567, 663, 685, 782
HpyF10VI GCNNNNNNNGC 3 cut(s) 731, 785, 1359
HpyF3I CTNAG 3 cut(s) 338, 386, 1104
HpySE526I ACGT 3 cut(s) 306, 741, 1057
Hsp92I GRCGYC 2 cut(s) 123, 741
Hsp92II CATG 5 cut(s) 214, 779, 1022, 1045, 1281
HspAI GCGC 2 cut(s) 831, 1248
Kpn2I TCCGGA 2 cut(s) 94, 851
Ksp22I TGATCA 2 cut(s) 462, 924
Kzo9I GATC 6 cut(s) 436, 462, 481, 676, 755, 924
LmnI GCTCC 1 cut(s) 643
Lsp1109I GCAGC 5 cut(s) 350, 716, 766, 794, 1288
LweI GCATC 6 cut(s) 36, 90, 226, 270, 1104, 1198
MaeI CTAG 4 cut(s) 485, 588, 759, 1154
MaeII ACGT 3 cut(s) 306, 741, 1057
MaeIII GTNAC 3 cut(s) 189, 583, 1021
MalI GATC 6 cut(s) 438, 464, 483, 678, 757, 926
MboI GATC 6 cut(s) 436, 462, 481, 676, 755, 924
MboII GAAGA 5 cut(s) 323, 374, 848, 961, 1142
MfeI CAATTG 1 cut(s) 147
MflI RGATCY 1 cut(s) 755
MhlI GDGCHC 2 cut(s) 472, 574
MlsI TGGCCA 1 cut(s) 1200
MluCI AATT 5 cut(s) 147, 762, 790, 855, 1008
MluNI TGGCCA 1 cut(s) 1200
MlyI GAGTC 5 cut(s) 70, 572, 790, 878, 881
MnlI CCTC 7 cut(s) 112, 429, 558, 634, 871, 946, 1049
Mox20I TGGCCA 1 cut(s) 1200
Mph1103I ATGCAT 1 cut(s) 263
MroI TCCGGA 2 cut(s) 94, 851
MroXI GAANNNNTTC 3 cut(s) 381, 1164, 1223
MscI TGGCCA 1 cut(s) 1200
MseI TTAA 8 cut(s) 443, 554, 599, 609, 770, 867, 1005, 1083
MslI CAYNNNNRTG 1 cut(s) 32
Msp20I TGGCCA 1 cut(s) 1200
MspA1I CMGCKG 2 cut(s) 922, 940
MspCI CTTAAG 1 cut(s) 1082
MspI CCGG 3 cut(s) 95, 656, 852
MspR9I CCNGG 2 cut(s) 656, 801
MunI CAATTG 1 cut(s) 147
Mva1269I GAATGC 2 cut(s) 263, 646
MvaI CCWGG 1 cut(s) 801
MvnI CGCG 1 cut(s) 1217
MwoI GCNNNNNNNGC 3 cut(s) 731, 785, 1359
NciI CCSGG 1 cut(s) 656
NdeII GATC 6 cut(s) 436, 462, 481, 676, 755, 924
NlaIII CATG 5 cut(s) 214, 779, 1022, 1045, 1281
NlaIV GGNNCC 1 cut(s) 977
NmuCI GTSAC 2 cut(s) 189, 583
NsiI ATGCAT 1 cut(s) 263
NspI RCATGY 1 cut(s) 214
PagI TCATGA 1 cut(s) 1041
PciI ACATGT 1 cut(s) 210
PcsI WCGNNNNNNNCGW 2 cut(s) 30, 1063
PctI GAATGC 2 cut(s) 263, 646
PdmI GAANNNNTTC 3 cut(s) 381, 1164, 1223
PfeI GAWTC 5 cut(s) 292, 431, 505, 805, 962
PflFI GACNNNGTC 2 cut(s) 581, 915
PkrI GCNGC 6 cut(s) 365, 706, 781, 784, 787, 1303
PleI GAGTC 5 cut(s) 70, 572, 790, 878, 880
PpsI GAGTC 5 cut(s) 70, 572, 790, 878, 880
PscI ACATGT 1 cut(s) 210
PshBI ATTAAT 1 cut(s) 443
Psp124BI GAGCTC 1 cut(s) 472
Psp6I CCWGG 1 cut(s) 799
PspFI CCCAGC 1 cut(s) 1354
PspGI CCWGG 1 cut(s) 799
PspN4I GGNNCC 1 cut(s) 977
PspPI GGNCC 2 cut(s) 152, 849
PstI CTGCAG 1 cut(s) 784
PstNI CAGNNNCTG 2 cut(s) 710, 881
PsuI RGATCY 1 cut(s) 755
PsyI GACNNNGTC 2 cut(s) 581, 915
PvuII CAGCTG 1 cut(s) 922
RsaI GTAC 1 cut(s) 653
RsaNI GTAC 1 cut(s) 652
RseI CAYNNNNRTG 1 cut(s) 32
Rsr2I CGGWCCG 1 cut(s) 849
RsrII CGGWCCG 1 cut(s) 849
SacI GAGCTC 1 cut(s) 472
SaqAI TTAA 8 cut(s) 443, 554, 599, 609, 770, 867, 1005, 1083
SatI GCNGC 6 cut(s) 364, 705, 780, 783, 786, 1302
Sau3AI GATC 6 cut(s) 436, 462, 481, 676, 755, 924
Sau96I GGNCC 2 cut(s) 152, 849
SchI GAGTC 5 cut(s) 70, 572, 790, 878, 881
ScrFI CCNGG 2 cut(s) 656, 801
SduI GDGCHC 2 cut(s) 472, 574
SfaNI GCATC 6 cut(s) 36, 90, 226, 270, 1104, 1198
SfcI CTRYAG 2 cut(s) 85, 780
SinI GGWCC 2 cut(s) 152, 849
SmiMI CAYNNNNRTG 1 cut(s) 32
SmlI CTYRAG 2 cut(s) 420, 1082
SmoI CTYRAG 2 cut(s) 420, 1082
SpeI ACTAGT 1 cut(s) 587
Sse9I AATT 5 cut(s) 147, 762, 790, 855, 1008
SsiI CCGC 2 cut(s) 786, 938
SspI AATATT 2 cut(s) 812, 1130
SspMI CTAG 4 cut(s) 485, 588, 759, 1154
SstI GAGCTC 1 cut(s) 472
StyD4I CCNGG 2 cut(s) 654, 799
TaaI ACNGT 3 cut(s) 849, 917, 1327
TaiI ACGT 3 cut(s) 309, 744, 1060
TaqI TCGA 3 cut(s) 21, 106, 309
TaqII GACCGA 1 cut(s) 571
TasI AATT 5 cut(s) 147, 762, 790, 855, 1008
TauI GCSGC 1 cut(s) 788
TfiI GAWTC 5 cut(s) 292, 431, 505, 805, 962
Tru1I TTAA 8 cut(s) 443, 554, 599, 609, 770, 867, 1005, 1083
Tru9I TTAA 8 cut(s) 443, 554, 599, 609, 770, 867, 1005, 1083
TseFI GTSAC 2 cut(s) 189, 583
TseI GCWGC 5 cut(s) 363, 704, 779, 782, 1301
Tsp45I GTSAC 2 cut(s) 189, 583
TspDTI ATGAA 7 cut(s) 270, 358, 934, 1058, 1173, 1285, 1306
TspGWI ACGGA 1 cut(s) 1187
Tth111I GACNNNGTC 2 cut(s) 581, 915
Vha464I CTTAAG 1 cut(s) 1082
VpaK11BI GGWCC 2 cut(s) 152, 849
VspI ATTAAT 1 cut(s) 443
XbaI TCTAGA 2 cut(s) 758, 1153
XceI RCATGY 1 cut(s) 214
XmnI GAANNNNTTC 3 cut(s) 381, 1164, 1223
XspI CTAG 4 cut(s) 485, 588, 759, 1154
ZraI GACGTC 1 cut(s) 742
Zsp2I ATGCAT 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.