Rh3BG120400

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0008870
Physical Location & Seq
Forward (+)
9951696 .. 9954316
2621 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG120400.1

Sequence Viewer

Length: 738 bp
ATGTTTAAATTTTCCGGGCTCCCCTCGCTCAGGTACTTAGAGATACTGACGCGGATGAAGGTGGATGTCGACAATAAACTCGACAGCATCCCCGTGTCGCGTCTCAACACAGAGAAAGAGATAAGAGTGGAGACTCGCATCTGTAGAGTTCCTGAGAAACTTCGAGAACTGAAGGAGGATGCCTACAATCCCCGAGTTGTTTCAATTGGACCTTTCCATTCCTGTAAGGATTACCTTGCACCAATGTCACGACATAAATACTATTACATGTCCCTTCTCATTAACAGCAAAGATGCTGAAAACTGCTTGGATGAATGCATCTGCGCTATTACTGATTTTGACGTTGTGATTCGTCAATGCTACGTCGAAGATATCAGTGTTTCAAATGATGAGCTTAGACTAATGATGTTAGTTGATGGCTGCTTCATATTGGAACTTCTTCTCAGGTTAGCACCTAACTTGGAGTATATAATGCCACAAGAATACGAATCTGATCCATTAATAAACAATGCTTGGATGATCAGAGCTCTTTGGCACGATCTAGCATTGCTTGAAAACCAGATTCCCTTTTTTATTCTGGAAAAGTTATACGATATTATCAAGCCTCGTTTAATGAGCAAGTGCAGAGCACCAGATTCGGTCACTAGTCTTGTTTTAAGTTTCTTTAAGCCTATGATGAGCCAAAACTCAAATATAGATCGAGGAGCATTCGTACCCGGACTGCAAACATTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

245

Amino Acids

28.51

Weight (kDa)

5.37

Isoelectric Point (pI)

50.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 47 - 231 1.7e-48 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 69
AccII CGCG 2 cut(s) 52, 100
AciI CCGC 1 cut(s) 52
AclWI GGATC 1 cut(s) 488
AcsI RAATTY 1 cut(s) 8
AcuI CTGAAG 1 cut(s) 191
AfaI GTAC 2 cut(s) 35, 714
AfiI CCNNNNNNNGG 1 cut(s) 30
AflIII ACRYGT 1 cut(s) 267
AgsI TTSAA 3 cut(s) 204, 384, 554
AhlI ACTAGT 1 cut(s) 644
AluBI AGCT 2 cut(s) 394, 527
AluI AGCT 2 cut(s) 394, 527
Alw21I GWGCWC 2 cut(s) 529, 631
Alw26I GTCTC 2 cut(s) 107, 125
AlwI GGATC 1 cut(s) 488
Ama87I CYCGRG 1 cut(s) 192
ApeKI GCWGC 1 cut(s) 420
ApoI RAATTY 1 cut(s) 8
AseI ATTAAT 1 cut(s) 500
Asp700I GAANNNNTTC 1 cut(s) 438
AspLEI GCGC 1 cut(s) 326
AspS9I GGNCC 1 cut(s) 209
AsuC2I CCSGG 2 cut(s) 16, 717
AvaI CYCGRG 1 cut(s) 192
AvaII GGWCC 1 cut(s) 209
BanII GRGCYC 2 cut(s) 21, 529
Bbv12I GWGCWC 2 cut(s) 529, 631
BbvI GCAGC 1 cut(s) 407
BccI CCATC 1 cut(s) 410
BcgI CGANNNNNNTGC 2 cut(s) 618, 652
BclI TGATCA 1 cut(s) 519
BcnI CCSGG 2 cut(s) 16, 717
BcoDI GTCTC 2 cut(s) 107, 125
BcuI ACTAGT 1 cut(s) 644
BfaI CTAG 2 cut(s) 542, 645
BfmI CTRYAG 1 cut(s) 142
BisI GCNGC 1 cut(s) 421
BlsI GCNGC 1 cut(s) 422
Bme1390I CCNGG 2 cut(s) 16, 717
Bme18I GGWCC 1 cut(s) 209
BmeT110I CYCGRG 1 cut(s) 192
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 1 cut(s) 20
BmrFI CCNGG 2 cut(s) 16, 717
BmsI GCATC 5 cut(s) 96, 147, 169, 283, 327
Bpu10I CCTNAGC 1 cut(s) 29
BpuMI CCSGG 2 cut(s) 16, 717
BsaXI ACNNNNNCTCC 2 cut(s) 696, 726
Bsc4I CCNNNNNNNGG 1 cut(s) 30
Bse3DI GCAATG 1 cut(s) 545
BseGI GGATG 6 cut(s) 60, 70, 87, 184, 316, 522
BseLI CCNNNNNNNGG 1 cut(s) 30
BseMI GCAATG 1 cut(s) 545
BseMII CTCAG 3 cut(s) 43, 144, 457
BseRI GAGGAG 1 cut(s) 717
BseXI GCAGC 1 cut(s) 407
BsgI GTGCAG 1 cut(s) 643
Bsh1236I CGCG 2 cut(s) 52, 100
BsiHKAI GWGCWC 2 cut(s) 529, 631
BsiHKCI CYCGRG 1 cut(s) 192
BsiSI CCGG 2 cut(s) 15, 717
BslFI GGGAC 1 cut(s) 256
BslI CCNNNNNNNGG 1 cut(s) 30
BsmAI GTCTC 2 cut(s) 107, 125
BsmBI CGTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 256
BsmI GAATGC 2 cut(s) 320, 707
BsoBI CYCGRG 1 cut(s) 192
Bsp1286I GDGCHC 3 cut(s) 21, 529, 631
Bsp143I GATC 4 cut(s) 493, 519, 538, 697
BspACI CCGC 1 cut(s) 52
BspCNI CTCAG 3 cut(s) 42, 145, 456
BspFNI CGCG 2 cut(s) 52, 100
BspLI GGNNCC 1 cut(s) 20
BspPI GGATC 1 cut(s) 488
BsrDI GCAATG 1 cut(s) 545
BssMI GATC 4 cut(s) 493, 519, 538, 697
BstDEI CTNAG 5 cut(s) 29, 37, 153, 395, 443
BstENI CCTNNNNNAGG 1 cut(s) 28
BstF5I GGATG 6 cut(s) 60, 70, 87, 184, 316, 522
BstFNI CGCG 2 cut(s) 52, 100
BstHHI GCGC 1 cut(s) 326
BstKTI GATC 4 cut(s) 496, 522, 541, 700
BstMAI GTCTC 2 cut(s) 107, 125
BstMBI GATC 4 cut(s) 493, 519, 538, 697
BstMWI GCNNNNNNNGC 1 cut(s) 25
BstNSI RCATGY 1 cut(s) 271
BstSCI CCNGG 2 cut(s) 14, 715
BstSFI CTRYAG 1 cut(s) 142
BstUI CGCG 2 cut(s) 52, 100
BstV1I GCAGC 1 cut(s) 407
BtsCI GGATG 6 cut(s) 60, 70, 87, 184, 316, 522
BtsIMutI CAGTG 1 cut(s) 382
CfoI GCGC 1 cut(s) 326
Cfr13I GGNCC 1 cut(s) 209
CseI GACGC 2 cut(s) 58, 89
Csp6I GTAC 2 cut(s) 34, 713
CviAII CATG 1 cut(s) 268
CviJI RGCY 7 cut(s) 19, 394, 420, 527, 604, 670, 681
CviKI_1 RGCY 7 cut(s) 19, 394, 420, 527, 604, 670, 681
CviQI GTAC 2 cut(s) 34, 713
DdeI CTNAG 5 cut(s) 29, 37, 153, 395, 443
DpnI GATC 4 cut(s) 495, 521, 540, 699
DpnII GATC 4 cut(s) 493, 519, 538, 697
DraI TTTAAA 1 cut(s) 7
Ecl136II GAGCTC 1 cut(s) 527
Eco24I GRGCYC 2 cut(s) 21, 529
Eco32I GATATC 1 cut(s) 373
Eco47I GGWCC 1 cut(s) 209
Eco53kI GAGCTC 1 cut(s) 527
Eco57I CTGAAG 1 cut(s) 191
Eco88I CYCGRG 1 cut(s) 192
EcoICRI GAGCTC 1 cut(s) 527
EcoNI CCTNNNNNAGG 1 cut(s) 28
EcoRV GATATC 1 cut(s) 373
EcoT22I ATGCAT 1 cut(s) 320
EcoT38I GRGCYC 2 cut(s) 21, 529
Esp3I CGTCTC 1 cut(s) 107
FaeI CATG 1 cut(s) 271
FaiI YATR 8 cut(s) 255, 269, 428, 468, 470, 589, 674, 695
FaqI GGGAC 1 cut(s) 256
FatI CATG 1 cut(s) 267
FbaI TGATCA 1 cut(s) 519
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 1 cut(s) 421
FokI GGATG 6 cut(s) 67, 74, 77, 191, 323, 529
FriOI GRGCYC 2 cut(s) 21, 529
Fsp4HI GCNGC 1 cut(s) 421
FspBI CTAG 2 cut(s) 542, 645
GlaI GCGC 1 cut(s) 325
GluI GCNGC 1 cut(s) 421
HapII CCGG 2 cut(s) 15, 717
HgaI GACGC 2 cut(s) 58, 89
HhaI GCGC 1 cut(s) 326
Hin1II CATG 1 cut(s) 271
Hin6I GCGC 1 cut(s) 324
HinP1I GCGC 1 cut(s) 324
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 5 cut(s) 133, 349, 488, 562, 635
HpaII CCGG 2 cut(s) 15, 717
Hpy166II GTNNAC 1 cut(s) 70
Hpy188I TCNGA 2 cut(s) 493, 524
Hpy188III TCNNGA 4 cut(s) 152, 164, 249, 578
Hpy8I GTNNAC 1 cut(s) 70
Hpy99I CGWCG 1 cut(s) 368
HpyAV CCTTC 3 cut(s) 52, 166, 284
HpyCH4IV ACGT 2 cut(s) 342, 363
HpyCH4V TGCA 4 cut(s) 239, 318, 624, 724
HpyF10VI GCNNNNNNNGC 1 cut(s) 25
HpyF3I CTNAG 5 cut(s) 29, 37, 153, 395, 443
HpySE526I ACGT 2 cut(s) 342, 363
Hsp92II CATG 1 cut(s) 271
HspAI GCGC 1 cut(s) 324
Ksp22I TGATCA 1 cut(s) 519
Kzo9I GATC 4 cut(s) 493, 519, 538, 697
LmnI GCTCC 2 cut(s) 24, 704
LpnPI CCDG 9 cut(s) 16, 28, 165, 235, 430, 563, 572, 645, 730
Lsp1109I GCAGC 1 cut(s) 407
LweI GCATC 5 cut(s) 96, 147, 169, 283, 327
MaeI CTAG 2 cut(s) 542, 645
MaeII ACGT 2 cut(s) 342, 363
MaeIII GTNAC 2 cut(s) 246, 640
MalI GATC 4 cut(s) 495, 521, 540, 699
MboI GATC 4 cut(s) 493, 519, 538, 697
MboII GAAGA 2 cut(s) 380, 431
MfeI CAATTG 1 cut(s) 204
MhlI GDGCHC 3 cut(s) 21, 529, 631
MluCI AATT 2 cut(s) 8, 204
MlyI GAGTC 1 cut(s) 127
MnlI CCTC 4 cut(s) 34, 169, 615, 695
Mph1103I ATGCAT 1 cut(s) 320
MroXI GAANNNNTTC 1 cut(s) 438
MseI TTAA 6 cut(s) 6, 282, 500, 611, 656, 666
MslI CAYNNNNRTG 1 cut(s) 92
MspI CCGG 2 cut(s) 15, 717
MspR9I CCNGG 2 cut(s) 16, 717
MunI CAATTG 1 cut(s) 204
Mva1269I GAATGC 2 cut(s) 320, 707
MvnI CGCG 2 cut(s) 52, 100
MwoI GCNNNNNNNGC 1 cut(s) 25
NciI CCSGG 2 cut(s) 16, 717
NdeII GATC 4 cut(s) 493, 519, 538, 697
NlaIII CATG 1 cut(s) 271
NlaIV GGNNCC 1 cut(s) 20
NmuCI GTSAC 2 cut(s) 246, 640
NsiI ATGCAT 1 cut(s) 320
NspI RCATGY 1 cut(s) 271
PciI ACATGT 1 cut(s) 267
PctI GAATGC 2 cut(s) 320, 707
PdmI GAANNNNTTC 1 cut(s) 438
PfeI GAWTC 4 cut(s) 349, 488, 562, 635
PkrI GCNGC 1 cut(s) 422
PleI GAGTC 1 cut(s) 127
PpsI GAGTC 1 cut(s) 127
PscI ACATGT 1 cut(s) 267
PshBI ATTAAT 1 cut(s) 500
Psp124BI GAGCTC 1 cut(s) 529
PspN4I GGNNCC 1 cut(s) 20
PspPI GGNCC 1 cut(s) 209
RsaI GTAC 2 cut(s) 35, 714
RsaNI GTAC 2 cut(s) 34, 713
RseI CAYNNNNRTG 1 cut(s) 92
SacI GAGCTC 1 cut(s) 529
SalI GTCGAC 1 cut(s) 68
SaqAI TTAA 6 cut(s) 6, 282, 500, 611, 656, 666
SatI GCNGC 1 cut(s) 421
Sau3AI GATC 4 cut(s) 493, 519, 538, 697
Sau96I GGNCC 1 cut(s) 209
SchI GAGTC 1 cut(s) 127
ScrFI CCNGG 2 cut(s) 16, 717
SduI GDGCHC 3 cut(s) 21, 529, 631
SfaNI GCATC 5 cut(s) 96, 147, 169, 283, 327
SfcI CTRYAG 1 cut(s) 142
SinI GGWCC 1 cut(s) 209
SmiMI CAYNNNNRTG 1 cut(s) 92
SpeI ACTAGT 1 cut(s) 644
Sse9I AATT 2 cut(s) 8, 204
SsiI CCGC 1 cut(s) 52
SspMI CTAG 2 cut(s) 542, 645
SstI GAGCTC 1 cut(s) 529
StyD4I CCNGG 2 cut(s) 14, 715
TaiI ACGT 2 cut(s) 345, 366
TaqI TCGA 5 cut(s) 69, 81, 163, 366, 700
TaqII GACCGA 1 cut(s) 628
TasI AATT 2 cut(s) 8, 204
TfiI GAWTC 4 cut(s) 349, 488, 562, 635
Tru1I TTAA 6 cut(s) 6, 282, 500, 611, 656, 666
Tru9I TTAA 6 cut(s) 6, 282, 500, 611, 656, 666
TscAI CASTG 1 cut(s) 382
TseFI GTSAC 2 cut(s) 246, 640
TseI GCWGC 1 cut(s) 420
Tsp45I GTSAC 2 cut(s) 246, 640
TspDTI ATGAA 3 cut(s) 71, 327, 415
TspRI CASTG 1 cut(s) 382
VpaK11BI GGWCC 1 cut(s) 209
VspI ATTAAT 1 cut(s) 500
XagI CCTNNNNNAGG 1 cut(s) 28
XapI RAATTY 1 cut(s) 8
XceI RCATGY 1 cut(s) 271
XmiI GTMKAC 1 cut(s) 69
XmnI GAANNNNTTC 1 cut(s) 438
XspI CTAG 2 cut(s) 542, 645
Zsp2I ATGCAT 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.