Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0006977

Physical Location & Seq

Genomic Coordinates Forward (+)

42789594 .. 42799321

Gene Structure 9728 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025177

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3405 bp

ATGGAAAAGCACAAGCATCAGAATCCATCGAAGTCAGTGAACGACGCAGTGTACAAGCTCCAGCTGGCACTCCTCGACGGCGTCAAAACCCTAGACCACCTCTACTTAGCCGGCTCCATAATCTCCCGCTCCGACTACAACGACGTCGTGACGGAGCGATCCATCGCCGACCTATGCGGCTACCCTCTCTGCTCCAATGCCCTACCTTCCGAAGCTTCCCGTTCCCGCAAGGGTCACTATCGCATTTCGCTCAAGGAGCACAAGGTGTACGATCTCCGAGAGACGAAGCTGTACTGCTCCTCCAAGTGTGTGATCGACAGCAAGGCGTTTGCTCAGAGCTTGAGCGAGGAGAGGTGTGATATCTTGGATTTCGGAAAGGTCGAGAGGGTTTTGGGGCAATTTGGGGAAAAAGATGAAGAAGGGAAGAAGGAGATTGGGGATTTGGGGATGTCTAAGTTGAAGATTGAAGAAAAGAGTGAGACTTATAGTGGGAAATTGGAGGACTGGGTTGGTCCTTCGAATGCCATTGAAGGATATGTTCCGAAAAAAGATAGGGTTTCGAAGCCGTTGGGTTCGAAAAAGAGCAAACAAGGGTCGAAAGGGAAAGATACGAAGCCGAGTGGTGGTGGGAAACAATTAATTTTGAATGATATGGACTTCATGAGTACACTATTAGCTTGTGATGAATATAGTGTGTCCAAAATGCCACCAACTGTAGCGGGGAATAGTGTTGATACTACGTTAAAGGAACCAAAAGGAAAAGTTGGTGGTAAAGATTCAGAGAGCGGATTTAGTGTGTTGGAAACCTCGGCTGCTCCTAATAAGAGTGAAGTGGATGTGATGGATGTTGGGGATTTAGGTATGTCTAGGTTGAAGATTGAAGAGAAAGAGGAATCCTTTGTTGGTAAGGCAGAGAAATCGAGTGAGGCTAGACTGAGGTCTTCTCTTAAACATTCGGGGACAAAGAAACTTAGTCGCTCTGTTACTTGGGCTGACGAAAAGATTGATATTACGGGGCGGAGAAATCTTTGTGAGGTGAGAGATATGGAAGATGGAATAGAAAATCCTGGTGCATTTGGCAACTTGCACAAACCTTCTACGTCAAATGAAGCCAGTTCCTCTTTTAATTGGGTTGATAAGACGATTGATAGTACTAACAGTGAAAATATTTGTGAGGTTAGAGAAATGCAAGATGCAAAATATGTTCCTGAGGTATTAGGCAGTTCAGTCCTGCAAGATAATGAATGGTTTGAGTCGGCAGAAGCCTGTGCATCAGCATTGAGTCAGGCAGCTGGAGCTGTTGCAACTGGAGAATCTGATACTAATGATGCTGTGTCAAAAGCCGGGATTATTATATTGCCACGTCCGGATGGTGTGGATGAAGAAGAGTCCATAGTGGATGATGTGTATGAAGAAGATTCCATAGAGGATAGTGTGGATGAAGAAGAGTCTGCAGAGGATGTTGACATGCTTGAACCAGAACATGCTGTTTCAAAGTGGCCAAGAAAGCCCGAAAGTTCACAGTATGATTTATTTAACCCTGAGGATTCTTGGTTTGATGCTCCACCAGATGGTTTCAATTTAACTTTATCTCCTTTTGCAACAATGTGGAATGCACTCTTTACATGGATAACATCATCTACATTGGCTTATATATATGGAAAGGATGATGGTTCCCATGAAGAGTTTCTCTACGTCAATGGGAGAAGTTATCCCCATAAGATTGTGTTGGCAGATGGTCGGTCTTCAGAAATCAAGCTAACTGTGGGTGAATCTCTTTCACGGGCTCTACCAGAAGTTGTTGCTGAACTAGGGCTACCAGTTTCCAGTTTAGAGAAAGGAATGGGCTTCATGTTGAATACAATGTCCTTTATTGAGGCGCTACCAGCATTCAGAATGAAACAGTGGCAAGTGATTGCTCTTCTTTTCATTGAGGGCCTCTCTGTTTGTAGGATCCCAGCACTTACTTCTCGCATGACAAATAGAAGGGTACTGATTCAGAAGGTGCTGGATGGTGCCCGTATAAGTGTAGAAGAGTACGAGATTATGAAGGATTTCCTAATACCGCTTGGCCGAGCGCCTCAATTCTCATCCCAGAGTGCTTCTTCGGTTGATGGTTGGTTTATCAAGGGAAAAACAAGAGACAGCGGAACAATTTTGGTAGGAATGGCTTCTGTGGGTGTGGCACCTACATCAGGTTTGAGAGAACCAAGTGGTCATGCTGTTGGCGTGGATAAGTTGCCCGAGGAGATGAATGACATGAAAATTAGGGATGACAAAGAAATGGAAGCTACTGTTACAGTTGTTGATGGTAATGGAACCGAGACGGGCCATATAATTGTGACGACAATTGGTGGTAGAAATGGTCAGCCAAAGCAGACAATTAGTTACATGGCTGAGCGTGTTGTCGGGCATGGATCATTTGGAGTTGTGTTCCAAGCAAAGTGCCTAGAGACTGGTGAAACTGTTGCAATAAAGAAGGTTCTGCAAGACAAGAGGTACAAAAACCGTGAGCTGCAAACCATGCGCTTGCTTGACCACCCAAATGTTGTATCTTTGAAGCATTGCTTCTTTTCAACAACTGAAAAGGATGAACTTTATCTCAACCTGGTACTTGAGTATGTTCCTGAAACAGTTCATCGTGTGATTAAACACTACAACAAGTTGAACCAGCGGATGCCCCTCATATATGTTAAGCTCTACACATACCAGATCTTTAGGGCATTATCCTACATTCACCGCTGCATTGGTGTGTGTCATCGGGACATTAAGCCTCAAAACCTTCTGGTGAATCCACATACCCACCAAGTAAAGTTGTGTGACTTTGGAAGTGCAAAAGTTTTGGTAAAAGGAGAGCCAAATATATCTTACATCTGCTCTAGATATTATAGAGCACCAGAACTCATATTTGGAGCAACTGAGTATACTACAGCTATTGACGTCTGGTCTGTTGGATGTGTTCTTGCTGAGCTATTGCTTGGGCAGCCTTTGTTTCCTGGTGAGAGTGGAGTCGACCAGCTTGTTGAGATTATCAAGGTATTGGGCACTCCAACAAGGGAGGAAATCAAATGCATGAACCCTAACTATACGGAGTTCAAATTCCCTCAAATTAAAGCTCACCCATGGCACAAGATTTTCCACAAGCGTATGCCTCCAGAAGCTGTTGACCTGGTTTCAAGACTATTGCAATACTCTCCTAACTTGCGGTGCACAGCTCTGGATGCCTTGACCCATCCCTTTTTCGATGAACTTCGTGACTTGAACACCCGCCTGCCAAATGGACGTTTCCTTCCACCTTTGTTCAACTTTAAATCTCATGAATTGAAGGGGGTCCCTGCTGAGATTTTGATGAAATTGGTCCCAGAGCATGCAAGAAAGCAAGTCCCATTCTTTGCGTCCGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0006357 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008420 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019219 GO:0019222 GO:0019538 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0036211 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070940 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RPAP2_Rtr1

Protein Analysis

1135

Amino Acids

126.25

Weight (kDa)

5.61

Isoelectric Point (pI)

43.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPAP2_Rtr1	PF04181	40 - 115	1.3e-22	Rtr1/RPAP2 family
Pkinase	PF00069	798 - 1081	2.8e-61	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	800 - 999	8.8e-24	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006977)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G26760 AT5G26760
fragaria_vesca	FvH4_6g08180 FvH4_6g08180 FvH4_6g08180 FvH4_6g08180 FvH4_6g08180 FvH4_6g08180
malus_domestica	MD04G1189300.v1.1 MD12G1202100.v1.1
prunus_persica	Prupe.6G306900_v2.0.a1
pyrus_communis	pycom04g16770 pycom12g18860
rosa_chinensis	RchiOBHm_Chr3g0458051
rosa_laevigata	RLG00000025177
rosa_multiflora	Rmu_sc0000384.1_g000014
rosa_roxburghii	Rroxscaffold_6G00421320
rosa_rugosa	Rorug03G0024600 Rorug03G0024600
rosa_samantha	Rh3AG085100 Rh3BG087900 Rh3CG088400 Rh3DG088800
rosa_wichuraiana	Rw0G012430 Rw3G007250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	147, 2943
AccB1I	GGYRCC	2 cut(s)	2015, 2185
AccB7I	CCANNNNNTGG	1 cut(s)	1571
AccBSI	CCGCTC	2 cut(s)	129, 786
AccI	GTMKAC	2 cut(s)	2924, 3012
AccIII	TCCGGA	1 cut(s)	1366
AclWI	GGATC	4 cut(s)	153, 1948, 1961, 2425
AcoI	YGGCCR	2 cut(s)	1499, 2071
AcsI	RAATTY	1 cut(s)	3098
AcuI	CTGAAG	1 cut(s)	1731
AcyI	GRCGYC	3 cut(s)	81, 144, 2940
AdeI	CACNNNGTG	1 cut(s)	265
AfaI	GTAC	9 cut(s)	53, 269, 293, 667, 1153, 1992, 2039, 2501, 2613
AfiI	CCNNNNNNNGG	3 cut(s)	623, 1571, 2195
AhdI	GACNNNNNGTC	1 cut(s)	2944
AjiI	CACGTC	1 cut(s)	1364
AjnI	CCWGG	4 cut(s)	1066, 2607, 2995, 3168
AjuI	GAANNNNNNNTTGG	4 cut(s)	885, 917, 2892, 2924
Alw21I	GWGCWC	3 cut(s)	261, 2896, 3212
Alw26I	GTCTC	5 cut(s)	275, 473, 2136, 2318, 2447
Alw44I	GTGCAC	1 cut(s)	3208
AlwI	GGATC	4 cut(s)	153, 1948, 1961, 2425
AlwNI	CAGNNNCTG	1 cut(s)	3161
Ama87I	CYCGRG	1 cut(s)	2243
Aor13HI	TCCGGA	1 cut(s)	1366
AoxI	GGCC	4 cut(s)	1499, 1936, 2071, 2329
ApaLI	GTGCAC	1 cut(s)	3208
ApeKI	GCWGC	5 cut(s)	812, 1289, 2515, 2742, 2983
ApoI	RAATTY	1 cut(s)	3098
AseI	ATTAAT	1 cut(s)	638
Asp700I	GAANNNNTTC	4 cut(s)	1686, 2054, 2170, 2634
AspLEI	GCGC	3 cut(s)	1882, 2080, 2529
AspS9I	GGNCC	5 cut(s)	512, 1936, 2329, 3331, 3358
AsuC2I	CCSGG	1 cut(s)	1345
AsuHPI	GGTGA	7 cut(s)	1048, 1781, 2471, 2729, 2800, 3011, 3110
AsuII	TTCGAA	3 cut(s)	518, 560, 575
AvaI	CYCGRG	1 cut(s)	2243
AvaII	GGWCC	3 cut(s)	512, 3331, 3358
AxyI	CCTNAGG	2 cut(s)	1209, 1542
BaeGI	GKGCMC	3 cut(s)	2020, 3047, 3212
BaeI	ACNNNNGTAYC	2 cut(s)	2603, 2636
BalI	TGGCCA	1 cut(s)	1501
BamHI	GGATCC	1 cut(s)	1953
BanI	GGYRCC	2 cut(s)	2015, 2185
BanII	GRGCYC	1 cut(s)	1789
BbsI	GAAGAC	2 cut(s)	933, 1737
Bbv12I	GWGCWC	3 cut(s)	261, 2896, 3212
BbvI	GCAGC	5 cut(s)	799, 1301, 2502, 2729, 2995
BceAI	ACGGC	2 cut(s)	94, 550
BcgI	CGANNNNNNTGC	2 cut(s)	900, 934
BciT130I	CCWGG	4 cut(s)	1068, 2609, 2997, 3170
BcnI	CCSGG	1 cut(s)	1345
BcoDI	GTCTC	5 cut(s)	275, 473, 2136, 2318, 2447
BfaI	CTAG	7 cut(s)	92, 867, 930, 1811, 2450, 2880, 3403
BfmI	CTRYAG	3 cut(s)	714, 1452, 2928
BfoI	RGCGCY	2 cut(s)	1883, 2081
BglII	AGATCT	1 cut(s)	2712
BisI	GCNGC	6 cut(s)	178, 813, 1290, 2516, 2743, 2984
BlpI	GCTNAGC	2 cut(s)	2397, 2967
BlsI	GCNGC	6 cut(s)	179, 814, 1291, 2517, 2744, 2985
BmcAI	AGTACT	1 cut(s)	1153
Bme1390I	CCNGG	5 cut(s)	1068, 1345, 2609, 2997, 3170
Bme18I	GGWCC	3 cut(s)	512, 3331, 3358
BmeRI	GACNNNNNGTC	1 cut(s)	2944
BmeT110I	CYCGRG	1 cut(s)	2243
BmgBI	CACGTC	1 cut(s)	1364
BmgT120I	GGNCC	5 cut(s)	512, 1936, 2329, 3331, 3358
BmrFI	CCNGG	5 cut(s)	1068, 1345, 2609, 2997, 3170
BmrI	ACTGGG	1 cut(s)	514
BmsI	GCATC	7 cut(s)	25, 1183, 1280, 1318, 1549, 2667, 3211
BmuI	ACTGGG	1 cut(s)	514
BoxI	GACNNNNGTC	1 cut(s)	937
BpiI	GAAGAC	2 cut(s)	933, 1737
BplI	GAGNNNNNCTC	4 cut(s)	928, 960, 1925, 1957
BpmI	CTGGAG	4 cut(s)	44, 1314, 1329, 3138
Bpu1102I	GCTNAGC	2 cut(s)	2397, 2967
Bpu14I	TTCGAA	3 cut(s)	518, 560, 575
BpuEI	CTTGAG	3 cut(s)	236, 361, 2636
BpuMI	CCSGG	1 cut(s)	1345
BsaHI	GRCGYC	3 cut(s)	81, 144, 2940
BsaJI	CCNNGG	3 cut(s)	807, 2244, 3122
BsaWI	WCCGGW	1 cut(s)	1366
BsaXI	ACNNNNNCTCC	4 cut(s)	284, 314, 1576, 1606
Bsc4I	CCNNNNNNNGG	3 cut(s)	623, 1571, 2195
Bse118I	RCCGGY	1 cut(s)	110
Bse1I	ACTGG	6 cut(s)	509, 1113, 1312, 1820, 1827, 2461
Bse21I	CCTNAGG	2 cut(s)	1209, 1542
Bse3DI	GCAATG	1 cut(s)	2563
BseAI	TCCGGA	1 cut(s)	1366
BseBI	CCWGG	4 cut(s)	1068, 2609, 2997, 3170
BseDI	CCNNGG	3 cut(s)	807, 2244, 3122
BseLI	CCNNNNNNNGG	3 cut(s)	623, 1571, 2195
BseMI	GCAATG	1 cut(s)	2563
BseMII	CTCAG	8 cut(s)	347, 926, 1200, 1533, 2388, 2910, 2958, 3330
BseNI	ACTGG	6 cut(s)	509, 1113, 1312, 1820, 1827, 2461
BseRI	GAGGAG	4 cut(s)	62, 289, 362, 2261
BseSI	GKGCMC	3 cut(s)	2020, 3047, 3212
BseXI	GCAGC	5 cut(s)	799, 1301, 2502, 2729, 2995
BseYI	CCCAGC	1 cut(s)	1957
BshFI	GGCC	4 cut(s)	1501, 1938, 2073, 2331
BshNI	GGYRCC	2 cut(s)	2015, 2185
BsiHKAI	GWGCWC	3 cut(s)	261, 2896, 3212
BsiHKCI	CYCGRG	1 cut(s)	2243
BsiSI	CCGG	3 cut(s)	111, 1344, 1367
BslFI	GGGAC	5 cut(s)	973, 2777, 3317, 3344, 3368
BslI	CCNNNNNNNGG	3 cut(s)	623, 1571, 2195
BsmAI	GTCTC	5 cut(s)	275, 473, 2136, 2318, 2447
BsmBI	CGTCTC	2 cut(s)	275, 2318
BsmFI	GGGAC	5 cut(s)	973, 2777, 3317, 3344, 3368
BsmI	GAATGC	3 cut(s)	526, 1618, 1889
BsnI	GGCC	4 cut(s)	1501, 1938, 2073, 2331
BsoBI	CYCGRG	1 cut(s)	2243
Bsp119I	TTCGAA	3 cut(s)	518, 560, 575
Bsp1286I	GDGCHC	6 cut(s)	261, 1789, 2020, 2896, 3047, 3212
Bsp13I	TCCGGA	1 cut(s)	1366
Bsp1407I	TGTACA	1 cut(s)	51
Bsp143I	GATC	6 cut(s)	158, 271, 312, 1953, 2417, 2712
Bsp1720I	GCTNAGC	2 cut(s)	2397, 2967
Bsp19I	CCATGG	1 cut(s)	3122
BspANI	GGCC	4 cut(s)	1501, 1938, 2073, 2331
BspCNI	CTCAG	8 cut(s)	346, 927, 1201, 1534, 2389, 2911, 2959, 3331
BspEI	TCCGGA	1 cut(s)	1366
BspHI	TCATGA	2 cut(s)	660, 3316
BspMAI	CTGCAG	1 cut(s)	1456
BspPI	GGATC	4 cut(s)	153, 1948, 1961, 2425
BspQI	GCTCTTC	1 cut(s)	1926
BspT104I	TTCGAA	3 cut(s)	518, 560, 575
BspT107I	GGYRCC	2 cut(s)	2015, 2185
BsrBI	CCGCTC	2 cut(s)	129, 786
BsrDI	GCAATG	1 cut(s)	2563
BsrFI	RCCGGY	1 cut(s)	110
BsrGI	TGTACA	1 cut(s)	51
BsrI	ACTGG	6 cut(s)	509, 1113, 1312, 1820, 1827, 2461
BssAI	RCCGGY	1 cut(s)	110
BssECI	CCNNGG	3 cut(s)	807, 2244, 3122
BssMI	GATC	6 cut(s)	158, 271, 312, 1953, 2417, 2712
BssNAI	GTATAC	1 cut(s)	2925
BssNI	GRCGYC	3 cut(s)	81, 144, 2940
BssT1I	CCWWGG	1 cut(s)	3122
Bst1107I	GTATAC	1 cut(s)	2925
Bst2UI	CCWGG	4 cut(s)	1068, 2609, 2997, 3170
Bst6I	CTCTTC	6 cut(s)	876, 1380, 1440, 1677, 1926, 2028
BstACI	GRCGYC	3 cut(s)	81, 144, 2940
BstAPI	GCANNNNNTGC	1 cut(s)	2524
BstAUI	TGTACA	1 cut(s)	51
BstBI	TTCGAA	3 cut(s)	518, 560, 575
BstC8I	GCNNGC	5 cut(s)	66, 112, 2531, 3272, 3369
BstDSI	CCRYGG	1 cut(s)	3122
BstENI	CCTNNNNNAGG	1 cut(s)	2193
BstH2I	RGCGCY	2 cut(s)	1883, 2081
BstHHI	GCGC	3 cut(s)	1882, 2080, 2529
BstKTI	GATC	6 cut(s)	161, 274, 315, 1956, 2420, 2715
BstMAI	GTCTC	5 cut(s)	275, 473, 2136, 2318, 2447
BstMBI	GATC	6 cut(s)	158, 271, 312, 1953, 2417, 2712
BstMWI	GCNNNNNNNGC	7 cut(s)	256, 1295, 1507, 1886, 2524, 2983, 3221
BstNI	CCWGG	4 cut(s)	1068, 2609, 2997, 3170
BstNSI	RCATGY	3 cut(s)	1471, 1487, 3371
BstPAI	GACNNNNGTC	1 cut(s)	937
BstSCI	CCNGG	5 cut(s)	1066, 1343, 2607, 2995, 3168
BstSFI	CTRYAG	3 cut(s)	714, 1452, 2928
BstSLI	GKGCMC	3 cut(s)	2020, 3047, 3212
BstV1I	GCAGC	5 cut(s)	799, 1301, 2502, 2729, 2995
BstV2I	GAAGAC	2 cut(s)	933, 1737
BstX2I	RGATCY	2 cut(s)	1953, 2712
BstYI	RGATCY	2 cut(s)	1953, 2712
BstZ17I	GTATAC	1 cut(s)	2925
Bsu36I	CCTNAGG	2 cut(s)	1209, 1542
BsuRI	GGCC	4 cut(s)	1501, 1938, 2073, 2331
BtgI	CCRYGG	1 cut(s)	3122
BtgZI	GCGATG	1 cut(s)	148
BtrI	CACGTC	1 cut(s)	1364
BtsI	GCAGTG	1 cut(s)	54
BtsIMutI	CAGTG	4 cut(s)	42, 54, 1165, 1910
Cac8I	GCNNGC	5 cut(s)	66, 112, 2531, 3272, 3369
CaiI	CAGNNNCTG	1 cut(s)	3161
CciI	TCATGA	2 cut(s)	660, 3316
CfoI	GCGC	3 cut(s)	1882, 2080, 2529
Cfr10I	RCCGGY	1 cut(s)	110
Cfr13I	GGNCC	5 cut(s)	512, 1936, 2329, 3331, 3358
CseI	GACGC	3 cut(s)	53, 70, 3384
CsiI	ACCWGGT	2 cut(s)	2607, 3168
Csp6I	GTAC	9 cut(s)	52, 268, 292, 666, 1152, 1991, 2038, 2500, 2612
CviQI	GTAC	9 cut(s)	52, 268, 292, 666, 1152, 1991, 2038, 2500, 2612
DpnI	GATC	6 cut(s)	160, 273, 314, 1955, 2419, 2714
DpnII	GATC	6 cut(s)	158, 271, 312, 1953, 2417, 2712
DraI	TTTAAA	1 cut(s)	3310
DraIII	CACNNNGTG	1 cut(s)	265
DriI	GACNNNNNGTC	1 cut(s)	2944
EaeI	YGGCCR	2 cut(s)	1499, 2071
Eam1104I	CTCTTC	6 cut(s)	876, 1380, 1440, 1677, 1926, 2028
Eam1105I	GACNNNNNGTC	1 cut(s)	2944
EarI	CTCTTC	6 cut(s)	876, 1380, 1440, 1677, 1926, 2028
EciI	GGCGGA	1 cut(s)	1033
Eco130I	CCWWGG	1 cut(s)	3122
Eco24I	GRGCYC	1 cut(s)	1789
Eco32I	GATATC	1 cut(s)	361
Eco47I	GGWCC	3 cut(s)	512, 3331, 3358
Eco57I	CTGAAG	1 cut(s)	1731
Eco81I	CCTNAGG	2 cut(s)	1209, 1542
Eco88I	CYCGRG	1 cut(s)	2243
EcoNI	CCTNNNNNAGG	1 cut(s)	2193
EcoO109I	RGGNCCY	2 cut(s)	1936, 3331
EcoRII	CCWGG	4 cut(s)	1066, 2607, 2995, 3168
EcoRV	GATATC	1 cut(s)	361
EcoT14I	CCWWGG	1 cut(s)	3122
EcoT22I	ATGCAT	1 cut(s)	3074
EcoT38I	GRGCYC	1 cut(s)	1789
ErhI	CCWWGG	1 cut(s)	3122
Esp3I	CGTCTC	2 cut(s)	275, 2318
FalI	AAGNNNNNCTT	2 cut(s)	2552, 2584
FaqI	GGGAC	5 cut(s)	973, 2777, 3317, 3344, 3368
FauI	CCCGC	4 cut(s)	134, 233, 712, 3275
FblI	GTMKAC	2 cut(s)	2924, 3012
Fnu4HI	GCNGC	6 cut(s)	178, 813, 1290, 2516, 2743, 2984
FriOI	GRGCYC	1 cut(s)	1789
Fsp4HI	GCNGC	6 cut(s)	178, 813, 1290, 2516, 2743, 2984
FspBI	CTAG	7 cut(s)	92, 867, 930, 1811, 2450, 2880, 3403
GlaI	GCGC	3 cut(s)	1881, 2079, 2528
GluI	GCNGC	6 cut(s)	178, 813, 1290, 2516, 2743, 2984
GsaI	CCCAGC	1 cut(s)	1961
GsuI	CTGGAG	4 cut(s)	44, 1314, 1329, 3138
HaeII	RGCGCY	2 cut(s)	1883, 2081
HaeIII	GGCC	4 cut(s)	1501, 1938, 2073, 2331
HapII	CCGG	3 cut(s)	111, 1344, 1367
HgaI	GACGC	3 cut(s)	53, 70, 3384
HhaI	GCGC	3 cut(s)	1882, 2080, 2529
Hin1I	GRCGYC	3 cut(s)	81, 144, 2940
Hin6I	GCGC	3 cut(s)	1880, 2078, 2527
HinP1I	GCGC	3 cut(s)	1880, 2078, 2527
HincII	GTYRAC	3 cut(s)	1465, 3013, 3166
HindII	GTYRAC	3 cut(s)	1465, 3013, 3166
HindIII	AAGCTT	1 cut(s)	213
HpaII	CCGG	3 cut(s)	111, 1344, 1367
HphI	GGTGA	7 cut(s)	1048, 1781, 2471, 2729, 2800, 3011, 3110
Hpy99I	CGWCG	4 cut(s)	47, 80, 146, 149
HpyCH4IV	ACGT	7 cut(s)	144, 740, 1100, 1363, 1695, 2940, 3283
HpyF10VI	GCNNNNNNNGC	7 cut(s)	256, 1295, 1507, 1886, 2524, 2983, 3221
HpySE526I	ACGT	7 cut(s)	144, 740, 1100, 1363, 1695, 2940, 3283
Hsp92I	GRCGYC	3 cut(s)	81, 144, 2940
HspAI	GCGC	3 cut(s)	1880, 2078, 2527
KflI	GGGWCCC	1 cut(s)	3331
Kpn2I	TCCGGA	1 cut(s)	1366
KroI	GCCGGC	1 cut(s)	110
KroNI	GCCGGC	1 cut(s)	112
Kzo9I	GATC	6 cut(s)	158, 271, 312, 1953, 2417, 2712
LguI	GCTCTTC	1 cut(s)	1926
Lsp1109I	GCAGC	5 cut(s)	799, 1301, 2502, 2729, 2995
LweI	GCATC	7 cut(s)	25, 1183, 1280, 1318, 1549, 2667, 3211
MabI	ACCWGGT	2 cut(s)	2607, 3168
MaeI	CTAG	7 cut(s)	92, 867, 930, 1811, 2450, 2880, 3403
MaeII	ACGT	7 cut(s)	144, 740, 1100, 1363, 1695, 2940, 3283
MaeIII	GTNAC	8 cut(s)	148, 233, 982, 2296, 2341, 2387, 2819, 3254
MalI	GATC	6 cut(s)	160, 273, 314, 1955, 2419, 2714
MbiI	CCGCTC	2 cut(s)	129, 786
MboI	GATC	6 cut(s)	158, 271, 312, 1953, 2417, 2712
MfeI	CAATTG	1 cut(s)	2349
MflI	RGATCY	2 cut(s)	1953, 2712
MhlI	GDGCHC	6 cut(s)	261, 1789, 2020, 2896, 3047, 3212
MlsI	TGGCCA	1 cut(s)	1501
MluNI	TGGCCA	1 cut(s)	1501
MlyI	GAGTC	5 cut(s)	1262, 1291, 1397, 1457, 3018
MmeI	TCCRAC	4 cut(s)	156, 780, 2932, 3074
Mox20I	TGGCCA	1 cut(s)	1501
Mph1103I	ATGCAT	1 cut(s)	3074
MroI	TCCGGA	1 cut(s)	1366
MroNI	GCCGGC	1 cut(s)	110
MroXI	GAANNNNTTC	4 cut(s)	1686, 2054, 2170, 2634
MscI	TGGCCA	1 cut(s)	1501
MslI	CAYNNNNRTG	2 cut(s)	2544, 2750
Msp20I	TGGCCA	1 cut(s)	1501
MspA1I	CMGCKG	5 cut(s)	64, 1292, 2148, 2674, 2742
MspI	CCGG	3 cut(s)	111, 1344, 1367
MspR9I	CCNGG	5 cut(s)	1068, 1345, 2609, 2997, 3170
MunI	CAATTG	1 cut(s)	2349
Mva1269I	GAATGC	3 cut(s)	526, 1618, 1889
MvaI	CCWGG	4 cut(s)	1068, 2609, 2997, 3170
MwoI	GCNNNNNNNGC	7 cut(s)	256, 1295, 1507, 1886, 2524, 2983, 3221
NaeI	GCCGGC	1 cut(s)	112
NciI	CCSGG	1 cut(s)	1345
NcoI	CCATGG	1 cut(s)	3122
NdeII	GATC	6 cut(s)	158, 271, 312, 1953, 2417, 2712
NgoMIV	GCCGGC	1 cut(s)	110
NmeAIII	GCCGAG	3 cut(s)	642, 788, 2099
NmuCI	GTSAC	5 cut(s)	148, 233, 2341, 2819, 3254
NsiI	ATGCAT	1 cut(s)	3074
NspI	RCATGY	3 cut(s)	1471, 1487, 3371
NspV	TTCGAA	3 cut(s)	518, 560, 575
PaeI	GCATGC	1 cut(s)	3371
PagI	TCATGA	2 cut(s)	660, 3316
PciSI	GCTCTTC	1 cut(s)	1926
PcsI	WCGNNNNNNNCGW	1 cut(s)	378
PctI	GAATGC	3 cut(s)	526, 1618, 1889
PdiI	GCCGGC	1 cut(s)	112
PdmI	GAANNNNTTC	4 cut(s)	1686, 2054, 2170, 2634
PfeI	GAWTC	9 cut(s)	22, 776, 893, 1313, 1418, 1547, 1772, 1996, 2791
PflFI	GACNNNGTC	1 cut(s)	80
PflMI	CCANNNNNTGG	1 cut(s)	1571
PkrI	GCNGC	6 cut(s)	179, 814, 1291, 2517, 2744, 2985
PleI	GAGTC	5 cut(s)	1261, 1290, 1396, 1456, 3017
PpsI	GAGTC	5 cut(s)	1261, 1290, 1396, 1456, 3017
PpuMI	RGGWCCY	1 cut(s)	3331
PshAI	GACNNNNGTC	1 cut(s)	937
PshBI	ATTAAT	1 cut(s)	638
Psp5II	RGGWCCY	1 cut(s)	3331
Psp6I	CCWGG	4 cut(s)	1066, 2607, 2995, 3168
PspFI	CCCAGC	1 cut(s)	1957
PspGI	CCWGG	4 cut(s)	1066, 2607, 2995, 3168
PspPI	GGNCC	5 cut(s)	512, 1936, 2329, 3331, 3358
PspPPI	RGGWCCY	1 cut(s)	3331
PsrI	GAACNNNNNNTAC	2 cut(s)	1800, 1832
PstI	CTGCAG	1 cut(s)	1456
PstNI	CAGNNNCTG	1 cut(s)	3161
PsuI	RGATCY	2 cut(s)	1953, 2712
PsyI	GACNNNGTC	1 cut(s)	80
PvuII	CAGCTG	2 cut(s)	64, 1292
RsaI	GTAC	9 cut(s)	53, 269, 293, 667, 1153, 1992, 2039, 2501, 2613
RsaNI	GTAC	9 cut(s)	52, 268, 292, 666, 1152, 1991, 2038, 2500, 2612
RseI	CAYNNNNRTG	2 cut(s)	2544, 2750
SalI	GTCGAC	1 cut(s)	3011
SapI	GCTCTTC	1 cut(s)	1926
SatI	GCNGC	6 cut(s)	178, 813, 1290, 2516, 2743, 2984
Sau3AI	GATC	6 cut(s)	158, 271, 312, 1953, 2417, 2712
Sau96I	GGNCC	5 cut(s)	512, 1936, 2329, 3331, 3358
ScaI	AGTACT	1 cut(s)	1153
SchI	GAGTC	5 cut(s)	1262, 1291, 1397, 1457, 3018
ScrFI	CCNGG	5 cut(s)	1068, 1345, 2609, 2997, 3170
SduI	GDGCHC	6 cut(s)	261, 1789, 2020, 2896, 3047, 3212
SexAI	ACCWGGT	2 cut(s)	2607, 3168
SfaNI	GCATC	7 cut(s)	25, 1183, 1280, 1318, 1549, 2667, 3211
SfcI	CTRYAG	3 cut(s)	714, 1452, 2928
SfuI	TTCGAA	3 cut(s)	518, 560, 575
SinI	GGWCC	3 cut(s)	512, 3331, 3358
SmiMI	CAYNNNNRTG	2 cut(s)	2544, 2750
SmlI	CTYRAG	3 cut(s)	251, 340, 2615
SmoI	CTYRAG	3 cut(s)	251, 340, 2615
SphI	GCATGC	1 cut(s)	3371
SspI	AATATT	1 cut(s)	1168
SspMI	CTAG	7 cut(s)	92, 867, 930, 1811, 2450, 2880, 3403
StyD4I	CCNGG	5 cut(s)	1066, 1343, 2607, 2995, 3168
StyI	CCWWGG	1 cut(s)	3122
TaiI	ACGT	7 cut(s)	147, 743, 1103, 1366, 1698, 2943, 3286
TaqII	GACCGA	1 cut(s)	1731
TatI	WGTACW	4 cut(s)	51, 291, 665, 1151
TauI	GCSGC	1 cut(s)	180
TfiI	GAWTC	9 cut(s)	22, 776, 893, 1313, 1418, 1547, 1772, 1996, 2791
TscAI	CASTG	4 cut(s)	42, 54, 1165, 1910
TseFI	GTSAC	5 cut(s)	148, 233, 2341, 2819, 3254
TseI	GCWGC	5 cut(s)	812, 1289, 2515, 2742, 2983
Tsp45I	GTSAC	5 cut(s)	148, 233, 2341, 2819, 3254
TspGWI	ACGGA	2 cut(s)	167, 3104
TspRI	CASTG	4 cut(s)	42, 54, 1165, 1910
Tth111I	GACNNNGTC	1 cut(s)	80
Van91I	CCANNNNNTGG	1 cut(s)	1571
VneI	GTGCAC	1 cut(s)	3208
VpaK11BI	GGWCC	3 cut(s)	512, 3331, 3358
VspI	ATTAAT	1 cut(s)	638
XagI	CCTNNNNNAGG	1 cut(s)	2193
XapI	RAATTY	1 cut(s)	3098
XbaI	TCTAGA	1 cut(s)	2879
XceI	RCATGY	3 cut(s)	1471, 1487, 3371
XmiI	GTMKAC	2 cut(s)	2924, 3012
XmnI	GAANNNNTTC	4 cut(s)	1686, 2054, 2170, 2634
XspI	CTAG	7 cut(s)	92, 867, 930, 1811, 2450, 2880, 3403
ZraI	GACGTC	2 cut(s)	145, 2941
ZrmI	AGTACT	1 cut(s)	1153
Zsp2I	ATGCAT	1 cut(s)	3074

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.