RLG00000025346

Respiratory burst

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005589
Physical Location & Seq
Reverse (-)
45193064 .. 45197867
4804 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025346

Sequence Viewer

Length: 2637 bp
ATGGAGATTCAAGCAGAAAACCCGCTGCCGGACAAAGAGAGCAAACGGGTCGGGCACAGCGGCCCGCTAGTGACCAGCAGCAAGATGAGGAGCAACAGCAACAGAAGCGCAAGGTTCAAAGAAGACGATCAGTGCTATGTGGAAGTGACACTGGATGTTTGCGACGACGATACCTTTGTGCTTCGGAACATCAGAGGAGCTGAACCTGAGTCTACGTCAGCAAGGCTAGAGAGAAGACCATCTCTTGCTTCTCAACTCTCTTTTAAACTCAAGAAAGTCTCGCATGAACTGAAGCGCATGAAATCTTCATTGAGCTTCAAGATGGTCGACAGGCCCACCAAGCCCGGTGTTGCTCGTGCCCTTAAGGGGCTCAAGCTCATGACCAAAAATGTGTGCAGTGAAGGTTGGCCAGAGATCGAAATAAGGTTTGATGAATTGGCCGTCGATGGAACTCTTCCAAAATCGGTCTTCGGTCAATGCATAGGGATGAAAGAGTCAAGTGAGTTTGCGGGCGAGATGTTTGATGCATTGGCTCGTCGGAGGGGAATAACATCGTGTTGTGTGACCAAGGAAGAGTTGAGAGAATTTTGGGAACAAATTTCAGATCACAGCTTCAATGCCAAGCTTCAAACGTTCTTTGACATGGTAGATAAAAATGCAGATGGTCGAATCTCTGAAGAAGAGGTGAAAGAGATTATTACCTTAAGTGCCTCTGCTAATAAGCTGTCAAAAATTCTAGAACAAGCTGAGGAATATGCAGCATTGATTATGGAAGAGCTTGATCAAGACAACCTTGGATATATTGATTTGTACAACTTGGAAATGCTACTTCTTTCGGGCCCAACCCAATCTTCGAACCTGGAAACAGTCAGCTACATTCAGAGCCGGTTGCTAAGTGAAAAGCTGGTTCCGACCAAAGAGCACAACCCTATAAGGCGGTGGTGCCGGCGGCTGTCCTACTTTGTTGAGGATAATTGGAGGCGGATTTGGGTCATTGTGCTATGGCTTTTGATATGCATGGGCCTCTTTGCTTGGAAATTCATTCAGTACAGACATAGGGAGGTCTTCCATGTAATGGGCTATTGTGTTTGCATTGCCAAAGGTGGAGCTGAGACCCTAAAGTTTAACATGGCCCTGATTCTTCTACCAGTATGTAGAAATACAATTACTTGGCTAAGAAGCAAGACCAAGTTAGGAGGGGTAGTTCCATTTGATGACAATATCAATTTTCATAAGGTGATCGCGTTTGGAATTGCAGTTGGAGTCGGTCTCCATGCCGGTTCCCATCTTACATGCGACTTTCCCAAGCTACTACACTCTACCAATGATCAATACGACCAGATGAAGCCATTTTTCGGCCAAAAACGACCCGACAACTACTGGTGGTTTGTGAAGGGAACCGAGGGCTGGACCGGGGTGGTTATGGTGGCGCTAATGCTAGTTGCATACACATTGGCTCAACCTTGGTTCCGTCGTAATCGACTGAGAAACCTTCCTAAAACCATAAAGAAACTTACTGGGTTCAATGCGTTTTGGTACTCCCATCACCTCTTTATCATTGTGTACATCCTCTTTATTATCCATGGATACTTTCTCTACCTCTCCAAGAATTGGGACAAGAAAACAACATGGATGTATCTAGCTTTCCCAGTAATAATGTATGCATGTGAGCGCTTGCTTCGGGCTTTCAGGTCTGGATATGAATCAGTGAAGATCTTAAAGGTCGCTATTTACCCAGGAAACGTATTGGCACTCCATATGTCTAAACCACAGGGATTAAAATATACCAGTGGACAGTACATTTACATAAATTGTTCTGCTATTTCCCCATTTCAATGGCATCCGTTCTCAATTACTTCAGCTCCTGGAGACGACTATCTAAGCGTTCATATTCGCACGTTGGGTGATTGGACATCTCAGCTCAAATACATTTTCTCTCAGGTTTGCCAACGTCCTCCTATAGAACACAGTGGCCTTCTAAGAGCTGATAATGAACAATCCAATATCCAACGCAGGATGCCAAGACTTCTTATTGATGGTCCATATGGTGCACCAGCACAAGACTACAAAAACTACGAAGTTCTTCTACTTGTTGGACTTGGAATTGGAGCAACTCCATTAATAAGCATACTCAAAGATGTGCTTAACAACATTAAGCGACAAAAAGAACTAGAAGCTCAAATGTTGGTGGAAAATGGTGTTAAGAGAAAGTCTACTTTTGCAACAAAACGAGCATATTTCTACTGGATCACAAGAGAGCAAGGCTCGTTTGAGTGGTTTAGGGGTGTGATGAATGAGGTCGCAGAAAATGACCTCGACGGCGTGATTGAGCTGCACAACTACTGCACCAGTGTTTATGAAGAAGGAGATGCTAGATCAGCTCTGATTACTATGCTTCAGTCTATAAACCATGCCAAGAATGGTGTGGATGTAGTTTCAGGAACGAGGGTGAAGACGCATTTTGCTAGACCGAATTGGCGTAATGTTTTTAAGCATGTGGCAGTTAAGCACCCTGATCAAAAAGTTGGAGTGTTTTATTGTGGTGCACATGGATTAGTTGGGGACTTAAAGAGGCTAGCTCAAGACTTTTCGAGAAAAACTGAGACCAAGTTTGATTTCCATAAAGAGAACTTTTAA

Protein Analysis

879

Amino Acids

100.93

Weight (kDa)

9.07

Isoelectric Point (pI)

43.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADPH_Ox PF08414 113 - 207 3.2e-33 Respiratory burst NADPH oxidase
EF-hand_7 PF13499 208 - 270 4.9e-06 EF-hand domain pair
Ferric_reduct PF01794 376 - 526 2.8e-11 Ferric reductase like transmembrane component
FAD_binding_8 PF08022 569 - 686 8.7e-36 FAD-binding domain
NAD_binding_6 PF08030 692 - 860 4.6e-51 Ferric reductase NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 942
AccB7I CCANNNNNTGG 2 cut(s) 1075, 1611
AccI GTMKAC 3 cut(s) 212, 327, 2213
AccII CGCG 1 cut(s) 1244
AciI CCGC 7 cut(s) 23, 60, 65, 509, 937, 949, 982
AclI AACGTT 1 cut(s) 632
AclWI GGATC 1 cut(s) 2255
AcoI YGGCCR 3 cut(s) 407, 438, 1357
AcsI RAATTY 4 cut(s) 584, 597, 732, 1037
AcuI CTGAAG 4 cut(s) 311, 696, 1842, 2381
AfaI GTAC 5 cut(s) 812, 1049, 1538, 1565, 1799
AfeI AGCGCT 1 cut(s) 1673
AfiI CCNNNNNNNGG 6 cut(s) 28, 366, 1075, 1355, 1407, 1611
AflII CTTAAG 2 cut(s) 362, 703
AgsI TTSAA 7 cut(s) 11, 118, 319, 616, 629, 1525, 1835
AjnI CCWGG 3 cut(s) 858, 1735, 1864
AjuI GAANNNNNNNTTGG 4 cut(s) 835, 867, 2600, 2632
AloI GAACNNNNNNTCC 2 cut(s) 1188, 1220
Alw21I GWGCWC 3 cut(s) 924, 2053, 2548
Alw26I GTCTC 5 cut(s) 283, 1106, 1274, 1863, 2597
Alw44I GTGCAC 2 cut(s) 2049, 2544
AlwI GGATC 1 cut(s) 2255
AlwNI CAGNNNCTG 1 cut(s) 1865
Aor51HI AGCGCT 1 cut(s) 1673
AoxI GGCC 9 cut(s) 61, 332, 407, 438, 838, 1021, 1131, 1357, 1972
ApaI GGGCCC 1 cut(s) 842
ApaLI GTGCAC 2 cut(s) 2049, 2544
ApeKI GCWGC 4 cut(s) 25, 78, 758, 2332
ApoI RAATTY 4 cut(s) 584, 597, 732, 1037
ArsI GACNNNNNNTTYG 2 cut(s) 158, 190
AseI ATTAAT 1 cut(s) 2120
Asp700I GAANNNNTTC 1 cut(s) 2082
AspLEI GCGC 4 cut(s) 110, 297, 1432, 1674
AspS9I GGNCC 8 cut(s) 62, 333, 838, 839, 1021, 1132, 1410, 2039
AsuC2I CCSGG 2 cut(s) 345, 1414
AsuHPI GGTGA 5 cut(s) 697, 1249, 1538, 1916, 2461
AsuII TTCGAA 1 cut(s) 854
AsuNHI GCTAGC 1 cut(s) 2575
AvaII GGWCC 2 cut(s) 1410, 2039
BaeGI GKGCMC 5 cut(s) 57, 361, 842, 2053, 2548
BaeI ACNNNNGTAYC 2 cut(s) 162, 195
BalI TGGCCA 1 cut(s) 409
BanI GGYRCC 1 cut(s) 942
BanII GRGCYC 2 cut(s) 372, 842
BarI GAAGNNNNNNTAC 2 cut(s) 2070, 2102
BauI CACGAG 1 cut(s) 354
BbsI GAAGAC 5 cut(s) 129, 241, 460, 1057, 2459
Bbv12I GWGCWC 3 cut(s) 924, 2053, 2548
BbvCI CCTCAGC 1 cut(s) 747
BbvI GCAGC 4 cut(s) 12, 90, 770, 2319
BccI CCATC 7 cut(s) 247, 316, 440, 656, 1293, 1551, 2030
BceAI ACGGC 2 cut(s) 425, 2335
BcgI CGANNNNNNTGC 2 cut(s) 31, 65
BciT130I CCWGG 3 cut(s) 860, 1737, 1866
BciVI GTATCC 1 cut(s) 1580
BclI TGATCA 3 cut(s) 781, 1327, 2515
BcnI CCSGG 2 cut(s) 345, 1414
BcoDI GTCTC 5 cut(s) 283, 1106, 1274, 1863, 2597
BfaI CTAG 9 cut(s) 68, 227, 737, 1439, 1640, 2171, 2373, 2466, 2576
BfmI CTRYAG 1 cut(s) 1959
BfoI RGCGCY 2 cut(s) 1433, 1675
BfrI CTTAAG 2 cut(s) 362, 703
BfuI GTATCC 1 cut(s) 1580
BglII AGATCT 1 cut(s) 1713
BisI GCNGC 6 cut(s) 26, 61, 79, 759, 950, 2333
BlsI GCNGC 6 cut(s) 27, 62, 80, 760, 951, 2334
Bme1390I CCNGG 5 cut(s) 345, 860, 1414, 1737, 1866
Bme18I GGWCC 2 cut(s) 1410, 2039
BmgT120I GGNCC 8 cut(s) 62, 333, 838, 839, 1021, 1132, 1410, 2039
BmiI GGNNCC 6 cut(s) 840, 909, 944, 1282, 1399, 1469
BmrFI CCNGG 5 cut(s) 345, 860, 1414, 1737, 1866
BmrI ACTGGG 2 cut(s) 1527, 1643
BmsI GCATC 4 cut(s) 514, 1849, 2007, 2359
BmtI GCTAGC 1 cut(s) 2579
BmuI ACTGGG 2 cut(s) 1527, 1643
BpiI GAAGAC 5 cut(s) 129, 241, 460, 1057, 2459
BplI GAGNNNNNCTC 6 cut(s) 1254, 1286, 2249, 2281, 2563, 2595
BpmI CTGGAG 1 cut(s) 1887
Bpu10I CCTNAGC 1 cut(s) 747
Bpu14I TTCGAA 1 cut(s) 854
BpuEI CTTGAG 3 cut(s) 254, 356, 2565
BpuMI CCSGG 2 cut(s) 345, 1414
BsaBI GATNNNNATC 1 cut(s) 1702
BsaI GGTCTC 3 cut(s) 1106, 1274, 2597
BsaJI CCNNGG 7 cut(s) 567, 793, 1401, 1413, 1463, 1582, 1735
BsaXI ACNNNNNCTCC 4 cut(s) 1188, 1218, 1846, 1876
Bsc4I CCNNNNNNNGG 6 cut(s) 28, 366, 1075, 1355, 1407, 1611
Bse118I RCCGGY 3 cut(s) 885, 945, 1277
Bse1I ACTGG 8 cut(s) 156, 1148, 1385, 1522, 1649, 1788, 2249, 2349
Bse3DI GCAATG 1 cut(s) 1092
Bse8I GATNNNNATC 1 cut(s) 1702
BseBI CCWGG 3 cut(s) 860, 1737, 1866
BseDI CCNNGG 7 cut(s) 567, 793, 1401, 1413, 1463, 1582, 1735
BseGI GGATG 7 cut(s) 160, 492, 1566, 1638, 1840, 2022, 2434
BseJI GATNNNNATC 1 cut(s) 1702
BseLI CCNNNNNNNGG 6 cut(s) 28, 366, 1075, 1355, 1407, 1611
BseMI GCAATG 1 cut(s) 1092
BseMII CTCAG 7 cut(s) 198, 738, 1101, 1475, 1931, 1952, 2592
BseNI ACTGG 8 cut(s) 156, 1148, 1385, 1522, 1649, 1788, 2249, 2349
BseRI GAGGAG 2 cut(s) 103, 210
BseSI GKGCMC 5 cut(s) 57, 361, 842, 2053, 2548
BseXI GCAGC 4 cut(s) 12, 90, 770, 2319
BsgI GTGCAG 3 cut(s) 415, 2318, 2329
Bsh1236I CGCG 1 cut(s) 1244
BshFI GGCC 9 cut(s) 63, 334, 409, 440, 840, 1023, 1133, 1359, 1974
BshNI GGYRCC 1 cut(s) 942
BsiHKAI GWGCWC 3 cut(s) 924, 2053, 2548
BsiSI CCGG 6 cut(s) 29, 345, 886, 946, 1278, 1413
BslFI GGGAC 2 cut(s) 1628, 2576
BslI CCNNNNNNNGG 6 cut(s) 28, 366, 1075, 1355, 1407, 1611
BsmAI GTCTC 5 cut(s) 283, 1106, 1274, 1863, 2597
BsmBI CGTCTC 1 cut(s) 1863
BsmFI GGGAC 2 cut(s) 1628, 2576
BsnI GGCC 9 cut(s) 63, 334, 409, 440, 840, 1023, 1133, 1359, 1974
Bso31I GGTCTC 3 cut(s) 1106, 1274, 2597
Bsp119I TTCGAA 1 cut(s) 854
Bsp120I GGGCCC 1 cut(s) 838
Bsp1286I GDGCHC 7 cut(s) 57, 361, 372, 842, 924, 2053, 2548
Bsp1407I TGTACA 2 cut(s) 810, 1563
Bsp19I CCATGG 1 cut(s) 1582
BspACI CCGC 7 cut(s) 23, 60, 65, 509, 937, 949, 982
BspANI GGCC 9 cut(s) 63, 334, 409, 440, 840, 1023, 1133, 1359, 1974
BspCNI CTCAG 7 cut(s) 199, 739, 1102, 1476, 1930, 1951, 2593
BspFNI CGCG 1 cut(s) 1244
BspHI TCATGA 1 cut(s) 378
BspLI GGNNCC 6 cut(s) 840, 909, 944, 1282, 1399, 1469
BspOI GCTAGC 1 cut(s) 2579
BspPI GGATC 1 cut(s) 2255
BspQI GCTCTTC 1 cut(s) 768
BspT104I TTCGAA 1 cut(s) 854
BspT107I GGYRCC 1 cut(s) 942
BspTI CTTAAG 2 cut(s) 362, 703
BspTNI GGTCTC 3 cut(s) 1106, 1274, 2597
BsrDI GCAATG 1 cut(s) 1092
BsrFI RCCGGY 3 cut(s) 885, 945, 1277
BsrGI TGTACA 2 cut(s) 810, 1563
BsrI ACTGG 8 cut(s) 156, 1148, 1385, 1522, 1649, 1788, 2249, 2349
BssAI RCCGGY 3 cut(s) 885, 945, 1277
BssECI CCNNGG 7 cut(s) 567, 793, 1401, 1413, 1463, 1582, 1735
BssSI CACGAG 1 cut(s) 354
BssT1I CCWWGG 4 cut(s) 567, 793, 1463, 1582
Bst2BI CACGAG 1 cut(s) 354
Bst2UI CCWGG 3 cut(s) 860, 1737, 1866
Bst4CI ACNGT 3 cut(s) 868, 1797, 1970
Bst6I CTCTTC 4 cut(s) 459, 567, 675, 768
BstAFI CTTAAG 2 cut(s) 362, 703
BstAUI TGTACA 2 cut(s) 810, 1563
BstBI TTCGAA 1 cut(s) 854
BstC8I GCNNGC 5 cut(s) 65, 511, 947, 1676, 2577
BstDSI CCRYGG 1 cut(s) 1582
BstF5I GGATG 7 cut(s) 160, 492, 1566, 1638, 1840, 2022, 2434
BstFNI CGCG 1 cut(s) 1244
BstH2I RGCGCY 2 cut(s) 1433, 1675
BstHHI GCGC 4 cut(s) 110, 297, 1432, 1674
BstMAI GTCTC 5 cut(s) 283, 1106, 1274, 1863, 2597
BstMWI GCNNNNNNNGC 3 cut(s) 105, 340, 2378
BstNI CCWGG 3 cut(s) 860, 1737, 1866
BstNSI RCATGY 3 cut(s) 1296, 1668, 2498
BstSCI CCNGG 5 cut(s) 343, 858, 1412, 1735, 1864
BstSFI CTRYAG 1 cut(s) 1959
BstSLI GKGCMC 5 cut(s) 57, 361, 842, 2053, 2548
BstUI CGCG 1 cut(s) 1244
BstV1I GCAGC 4 cut(s) 12, 90, 770, 2319
BstV2I GAAGAC 5 cut(s) 129, 241, 460, 1057, 2459
BstX2I RGATCY 1 cut(s) 1713
BstXI CCANNNNNNTGG 1 cut(s) 1836
BstYI RGATCY 1 cut(s) 1713
BsuI GTATCC 1 cut(s) 1580
BsuRI GGCC 9 cut(s) 63, 334, 409, 440, 840, 1023, 1133, 1359, 1974
BtgI CCRYGG 1 cut(s) 1582
BtsCI GGATG 7 cut(s) 160, 492, 1566, 1638, 1840, 2022, 2434
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 7 cut(s) 137, 149, 403, 1713, 1795, 1975, 2356
Cac8I GCNNGC 5 cut(s) 65, 511, 947, 1676, 2577
CaiI CAGNNNCTG 1 cut(s) 1865
CciI TCATGA 1 cut(s) 378
CfoI GCGC 4 cut(s) 110, 297, 1432, 1674
Cfr10I RCCGGY 3 cut(s) 885, 945, 1277
Cfr13I GGNCC 8 cut(s) 62, 333, 838, 839, 1021, 1132, 1410, 2039
CseI GACGC 1 cut(s) 2464
Csp6I GTAC 5 cut(s) 811, 1048, 1537, 1564, 1798
CviQI GTAC 5 cut(s) 811, 1048, 1537, 1564, 1798
DraI TTTAAA 1 cut(s) 265
EaeI YGGCCR 3 cut(s) 407, 438, 1357
Eam1104I CTCTTC 4 cut(s) 459, 567, 675, 768
EarI CTCTTC 4 cut(s) 459, 567, 675, 768
EciI GGCGGA 1 cut(s) 997
Eco130I CCWWGG 4 cut(s) 567, 793, 1463, 1582
Eco24I GRGCYC 2 cut(s) 372, 842
Eco31I GGTCTC 3 cut(s) 1106, 1274, 2597
Eco47I GGWCC 2 cut(s) 1410, 2039
Eco47III AGCGCT 1 cut(s) 1673
Eco57I CTGAAG 4 cut(s) 311, 696, 1842, 2381
EcoRII CCWGG 3 cut(s) 858, 1735, 1864
EcoT14I CCWWGG 4 cut(s) 567, 793, 1463, 1582
EcoT22I ATGCAT 4 cut(s) 482, 529, 1019, 1666
EcoT38I GRGCYC 2 cut(s) 372, 842
ErhI CCWWGG 4 cut(s) 567, 793, 1463, 1582
Esp3I CGTCTC 1 cut(s) 1863
FalI AAGNNNNNCTT 4 cut(s) 777, 809, 2127, 2159
FaqI GGGAC 2 cut(s) 1628, 2576
FauI CCCGC 3 cut(s) 30, 72, 502
FauNDI CATATG 2 cut(s) 1758, 2044
FbaI TGATCA 3 cut(s) 781, 1327, 2515
FblI GTMKAC 3 cut(s) 212, 327, 2213
Fnu4HI GCNGC 6 cut(s) 26, 61, 79, 759, 950, 2333
FokI GGATG 7 cut(s) 167, 499, 1553, 1645, 1827, 2029, 2441
FriOI GRGCYC 2 cut(s) 372, 842
Fsp4HI GCNGC 6 cut(s) 26, 61, 79, 759, 950, 2333
FspBI CTAG 9 cut(s) 68, 227, 737, 1439, 1640, 2171, 2373, 2466, 2576
GlaI GCGC 4 cut(s) 109, 296, 1431, 1673
GluI GCNGC 6 cut(s) 26, 61, 79, 759, 950, 2333
GsuI CTGGAG 1 cut(s) 1887
HaeII RGCGCY 2 cut(s) 1433, 1675
HaeIII GGCC 9 cut(s) 63, 334, 409, 440, 840, 1023, 1133, 1359, 1974
HapII CCGG 6 cut(s) 29, 345, 886, 946, 1278, 1413
HgaI GACGC 1 cut(s) 2464
HhaI GCGC 4 cut(s) 110, 297, 1432, 1674
Hin6I GCGC 4 cut(s) 108, 295, 1430, 1672
HinP1I GCGC 4 cut(s) 108, 295, 1430, 1672
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HindIII AAGCTT 1 cut(s) 623
HinfI GANTC 7 cut(s) 7, 209, 494, 669, 1138, 1263, 1703
HpaII CCGG 6 cut(s) 29, 345, 886, 946, 1278, 1413
HphI GGTGA 5 cut(s) 697, 1249, 1538, 1916, 2461
Hpy166II GTNNAC 7 cut(s) 213, 328, 1564, 1793, 2051, 2214, 2546
Hpy188I TCNGA 8 cut(s) 186, 194, 540, 604, 676, 882, 912, 2385
Hpy188III TCNNGA 9 cut(s) 271, 319, 379, 737, 785, 1695, 2439, 2582, 2592
Hpy8I GTNNAC 7 cut(s) 213, 328, 1564, 1793, 2051, 2214, 2546
Hpy99I CGWCG 6 cut(s) 167, 170, 446, 540, 1476, 2321
HpyAV CCTTC 5 cut(s) 395, 1387, 1502, 1985, 2357
HpyCH4III ACNGT 3 cut(s) 868, 1797, 1970
HpyCH4IV ACGT 5 cut(s) 215, 632, 1743, 1898, 1951
HpyF10VI GCNNNNNNNGC 3 cut(s) 105, 340, 2378
HpySE526I ACGT 5 cut(s) 215, 632, 1743, 1898, 1951
HspAI GCGC 4 cut(s) 108, 295, 1430, 1672
KroI GCCGGC 1 cut(s) 945
KroNI GCCGGC 1 cut(s) 947
Ksp22I TGATCA 3 cut(s) 781, 1327, 2515
LguI GCTCTTC 1 cut(s) 768
LmnI GCTCC 5 cut(s) 90, 197, 1106, 1867, 2108
Lsp1109I GCAGC 4 cut(s) 12, 90, 770, 2319
LweI GCATC 4 cut(s) 514, 1849, 2007, 2359
MaeI CTAG 9 cut(s) 68, 227, 737, 1439, 1640, 2171, 2373, 2466, 2576
MaeII ACGT 5 cut(s) 215, 632, 1743, 1898, 1951
MaeIII GTNAC 3 cut(s) 70, 145, 562
MflI RGATCY 1 cut(s) 1713
MhlI GDGCHC 7 cut(s) 57, 361, 372, 842, 924, 2053, 2548
MlsI TGGCCA 1 cut(s) 409
MluNI TGGCCA 1 cut(s) 409
MlyI GAGTC 3 cut(s) 218, 503, 1272
MmeI TCCRAC 6 cut(s) 518, 935, 1240, 2032, 2074, 2506
Mox20I TGGCCA 1 cut(s) 409
Mph1103I ATGCAT 4 cut(s) 482, 529, 1019, 1666
MroNI GCCGGC 1 cut(s) 945
MroXI GAANNNNTTC 1 cut(s) 2082
MscI TGGCCA 1 cut(s) 409
MslI CAYNNNNRTG 2 cut(s) 485, 1834
Msp20I TGGCCA 1 cut(s) 409
MspA1I CMGCKG 2 cut(s) 25, 60
MspCI CTTAAG 2 cut(s) 362, 703
MspI CCGG 6 cut(s) 29, 345, 886, 946, 1278, 1413
MspR9I CCNGG 5 cut(s) 345, 860, 1414, 1737, 1866
MvaI CCWGG 3 cut(s) 860, 1737, 1866
MvnI CGCG 1 cut(s) 1244
MwoI GCNNNNNNNGC 3 cut(s) 105, 340, 2378
NaeI GCCGGC 1 cut(s) 947
NciI CCSGG 2 cut(s) 345, 1414
NcoI CCATGG 1 cut(s) 1582
NdeI CATATG 2 cut(s) 1758, 2044
NgoMIV GCCGGC 1 cut(s) 945
NheI GCTAGC 1 cut(s) 2575
NlaIV GGNNCC 6 cut(s) 840, 909, 944, 1282, 1399, 1469
NmuCI GTSAC 3 cut(s) 70, 145, 562
NsiI ATGCAT 4 cut(s) 482, 529, 1019, 1666
NspI RCATGY 3 cut(s) 1296, 1668, 2498
NspV TTCGAA 1 cut(s) 854
PagI TCATGA 1 cut(s) 378
PciSI GCTCTTC 1 cut(s) 768
PdiI GCCGGC 1 cut(s) 947
PdmI GAANNNNTTC 1 cut(s) 2082
PfeI GAWTC 4 cut(s) 7, 669, 1138, 1703
PflMI CCANNNNNTGG 2 cut(s) 1075, 1611
PfoI TCCNGGA 1 cut(s) 1864
PkrI GCNGC 6 cut(s) 27, 62, 80, 760, 951, 2334
PleI GAGTC 3 cut(s) 217, 502, 1271
PpsI GAGTC 3 cut(s) 217, 502, 1271
PshBI ATTAAT 1 cut(s) 2120
Psp1406I AACGTT 1 cut(s) 632
Psp6I CCWGG 3 cut(s) 858, 1735, 1864
PspGI CCWGG 3 cut(s) 858, 1735, 1864
PspN4I GGNNCC 6 cut(s) 840, 909, 944, 1282, 1399, 1469
PspOMI GGGCCC 1 cut(s) 838
PspPI GGNCC 8 cut(s) 62, 333, 838, 839, 1021, 1132, 1410, 2039
PstNI CAGNNNCTG 1 cut(s) 1865
PsuI RGATCY 1 cut(s) 1713
RsaI GTAC 5 cut(s) 812, 1049, 1538, 1565, 1799
RsaNI GTAC 5 cut(s) 811, 1048, 1537, 1564, 1798
RseI CAYNNNNRTG 2 cut(s) 485, 1834
SalI GTCGAC 1 cut(s) 326
SapI GCTCTTC 1 cut(s) 768
SatI GCNGC 6 cut(s) 26, 61, 79, 759, 950, 2333
Sau96I GGNCC 8 cut(s) 62, 333, 838, 839, 1021, 1132, 1410, 2039
SchI GAGTC 3 cut(s) 218, 503, 1272
ScrFI CCNGG 5 cut(s) 345, 860, 1414, 1737, 1866
SduI GDGCHC 7 cut(s) 57, 361, 372, 842, 924, 2053, 2548
SfaNI GCATC 4 cut(s) 514, 1849, 2007, 2359
SfcI CTRYAG 1 cut(s) 1959
SfuI TTCGAA 1 cut(s) 854
SinI GGWCC 2 cut(s) 1410, 2039
SmiMI CAYNNNNRTG 2 cut(s) 485, 1834
SmlI CTYRAG 5 cut(s) 269, 362, 371, 703, 2580
SmoI CTYRAG 5 cut(s) 269, 362, 371, 703, 2580
SsiI CCGC 7 cut(s) 23, 60, 65, 509, 937, 949, 982
SspMI CTAG 9 cut(s) 68, 227, 737, 1439, 1640, 2171, 2373, 2466, 2576
StyD4I CCNGG 5 cut(s) 343, 858, 1412, 1735, 1864
StyI CCWWGG 4 cut(s) 567, 793, 1463, 1582
TaaI ACNGT 3 cut(s) 868, 1797, 1970
TaiI ACGT 5 cut(s) 218, 635, 1746, 1901, 1954
TaqI TCGA 8 cut(s) 327, 417, 444, 667, 854, 1480, 2316, 2591
TaqII GACCGA 4 cut(s) 454, 461, 1256, 2485
TatI WGTACW 4 cut(s) 810, 1047, 1563, 1797
TauI GCSGC 2 cut(s) 63, 952
TfiI GAWTC 4 cut(s) 7, 669, 1138, 1703
TscAI CASTG 7 cut(s) 137, 156, 403, 1713, 1795, 1975, 2356
TseFI GTSAC 3 cut(s) 70, 145, 562
TseI GCWGC 4 cut(s) 25, 78, 758, 2332
Tsp45I GTSAC 3 cut(s) 70, 145, 562
TspGWI ACGGA 2 cut(s) 1460, 1833
TspRI CASTG 7 cut(s) 137, 156, 403, 1713, 1795, 1975, 2356
Van91I CCANNNNNTGG 2 cut(s) 1075, 1611
Vha464I CTTAAG 2 cut(s) 362, 703
VneI GTGCAC 2 cut(s) 2049, 2544
VpaK11BI GGWCC 2 cut(s) 1410, 2039
VspI ATTAAT 1 cut(s) 2120
XapI RAATTY 4 cut(s) 584, 597, 732, 1037
XbaI TCTAGA 1 cut(s) 736
XceI RCATGY 3 cut(s) 1296, 1668, 2498
XcmI CCANNNNNNNNNTGG 2 cut(s) 2417, 2422
XmiI GTMKAC 3 cut(s) 212, 327, 2213
XmnI GAANNNNTTC 1 cut(s) 2082
XspI CTAG 9 cut(s) 68, 227, 737, 1439, 1640, 2171, 2373, 2466, 2576
Zsp2I ATGCAT 4 cut(s) 482, 529, 1019, 1666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.