Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0005589

Physical Location & Seq

Genomic Coordinates Forward (+)

4542383 .. 4547163

Gene Structure 4781 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G005480.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2508 bp

ATGGAGATTCAAGCAGAAAACCCGCTGCCGGATAAAGAGAGCAAACGGGTCGGGCAGGGCGGCCCGCTCGGGACCAGCAGCAAGATGAGGAGCAACAGCAACAGAAGCACAATGTTCAAAGAAGACGATCAATGCTATGTGGAAGTGGCACTCGATGGTTGCGACGACAATACCTTTGTGCTTCGGAACATCAGAGGAGCTGAACCTGAGTCGACGTCAGCAAGGCTAGAGAGAAGACGATCCGTTGCTTCTCAACTCTCTTTTAAACTCAAGAAAGTCACACGTGAACTGAAACGCATGAAATCTTCCTTGAGCTTCAAGAAGGTCGACAGGCCCACCAAGCCCGGTATTGCTCGTGCCCTTAAGGGACTCAAGCTCATGACCAAAAATGTGTGCGGTGAAGGTTGGCCAGAGATCGAAATAAGGTTTGATGAATTGGCTGTCGATGGAACTCTTCCAAAATCAGTCTTTGCTCAATGCATAGGGATGAAAGAGTCGAGTGAGTTTGCTTGCGAAGTATTCGATGCATTGGCTCGTCGGAGAGGAATAACATCGTGTTGTGTGACCAAGGAAGAGTTGAGAGAATTTTGGGAACAAATTTCAGATCACAGCTTCAATGCCAGGCTTCAAACCTTCTTTGACATGGTGGATAAAAATGCAGATGGTCGAATCTCTGAAGAAGAGGTGAAAGAGATTATTACCTTAAGTGCCTCTGCTAATAAGCTGTCAAAAATTCTAGAGCATGCTGAGGAATATGCAGCATTGATTATGGAAGAGCTTGATCATGACAACCTCGGATATATTGATTTGTACAACTTGAAAATGCTACTTCTTCCGGACCCAACCCAATCTTCAAACCTGGAAACAGACAGCTACATTCAGAGCCGGTTGCTAAGTGAAAAGCTGGTTCCGACCAAAGAACACAACCCTATAAGGCGGTGGTGCCGGCGGCTGTCCTACTTTGTTGAGGATAACTGGAGGCGGATTTGGGTCATTTATAGACATAGGGAGGTCTTCCATGTAATGGGCTATTGTGTGTGCATTGCAAAAGGTGGAGCTGAGACCCTCAAGTTCAACATGGCGCTAATTCTTCTACCGGTGTGCAGAAATACAATTACTTGGCTAAGAAGCAAGACCAATCTCCATGCTGGTTCCCATCTTACATGCGACTTCCCCAGGCTACTACATTCAACCAATGATCAATACGACAAAATGAAGCCATTTTTCGGCCAAAAACGACCCGACAACTACTGGTGGTTTATGAAGGGAACCGAGGGCTGGACCGGGGTGGTTATGGTGGCATTAATGCTGGTTGCATACACATTGGCTCAACCTTGGTTCCGTCGCAATCGACTAAGAAACCTCCCTAAAACCCTAAAGAAAGTTACTGGGTTCAATGCGTTTTGGTACTCCCATCACCTCTTTATCATTGTGTACATCCTCCTTATTATCCATGGATACTTCCTCTACCTCTCCAAGGACTGGGACAAGAAAACAACATGGATGTATCTGGCTTTCCCAGTAATAATGTATGCATGTGAGCGTTTGCTTCGTGCTTTCAGGTCTGGATATGAATCAGTGAAGATCTTAAAGGTTACTGTTTACCCGGGAAACGTATTGGCACTCCATATGTCAAAACCACAGGGATTAAAATATACCAGTGGACAGTACATTTACATAAATTGTTCTGCTATTTCCTCATTTCAATGGCATCCATTCTCAATTACTTCGGCTCCTGGAGACGACTATCTAAGTGTTCATATTCGCACGTTGGGTGATTGGACGTCTCAGCTCAAATACATTTTCTCTCAGGTTTGCGAACGTCCTCCTCTAGAACACAGTGGCCTTCTAAGAGCTGATACTGAACAACCCGATATACAACTCAGGATGCCAAGACTTCTTATTGATGGTCCATATGGTGCACCAGCTCAAGACTACAAAAACTACGAAGTTCTTCTACTTGTTGGACTTGGAATTGGAGCAACTCCATTAATAAGCATACTCAAAGATGTGCTTAACAACATTAAGCGACAGAAAGAACTAGAAGCTCAAATGTTGGTGGAAAGTGGTGTGAAGAGAAAGTCTACTTTTGCAACAAAACGAGCTTATTTCTACTGGATCACAAGAGAGCAAGGCTCGTTCGAGTGGTTTAGGGGTGTGATGAATGAGGTCGCAGAAAATGACCTCGACGGCGTGATTGAACTGCACAACTACTGCACCAGTGTCTATGAAGAAGGAGATGCTAGATCTGCTCTGATTACTATGCTTCAGTCTATAAACCATGCCAAAAATGGTGTGGATGTTGTATCAGGAACGAGGGTGAAGACGCATTTTGCAAGACCAAAATGGCGTAATGTTTTTAAGCATGTGGCGGTAAAGCATCCTGATCAAAAAGTTGGAGTGTTTTATTGTGGCGCACATGGATTAGTTGGGGAGTTAAAGAGGTTAGCTAAAGACTTTTCCAGAAAAACTGAGACCGAGTTTGATTTCCACAAAGAGAACTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005509 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009628 GO:0009791 GO:0009845 GO:0016020 GO:0016174 GO:0016491 GO:0016651 GO:0032501 GO:0032502 GO:0043167 GO:0043169 GO:0043621 GO:0044464 GO:0046872 GO:0048856 GO:0050664 GO:0050896 GO:0055114 GO:0071944 GO:0090351

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

EF-hand_6 FAD_binding_8 Ferric_reduct NADPH_Ox NAD_binding_6

Protein Analysis

835

Amino Acids

96.26

Weight (kDa)

9.04

Isoelectric Point (pI)

42.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NADPH_Ox	PF08414	113 - 207	2.5e-32	Respiratory burst NADPH oxidase
EF-hand_7	PF13499	208 - 270	6.2e-06	EF-hand domain pair
FAD_binding_8	PF08022	526 - 643	3.8e-35	FAD-binding domain
NAD_binding_6	PF08030	649 - 817	2.6e-50	Ferric reductase NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005589)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09090 AT1G09090
fragaria_vesca	FvH4_6g06490
prunus_persica	Prupe.6G321500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0455621 RchiOBHm_Chr3g0455641
rosa_laevigata	RLG00000025344 RLG00000025346
rosa_multiflora	Rmu_sc0000223.1_g000005 Rmu_sc0002883.1_g000005
rosa_roxburghii	Rroxscaffold_1G00052210 Rroxscaffold_5G00351700 Rroxscaffold_6G00423640 Rroxscaffold_6G00423670 Rroxscaffold_6G00424140 Rroxscaffold_6G00424160
rosa_rugosa	Rorug03G0008800 Rorug03G0008900
rosa_samantha	Rh3AG068200 Rh3AG068400 Rh3BG070800 Rh3BG070900 Rh3CG069100 Rh3CG069200 Rh3DG070700 Rh3DG070800
rosa_wichuraiana	Rw3G005470 Rw3G005480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	218, 1787
AccB1I	GGYRCC	1 cut(s)	942
AccB7I	CCANNNNNTGG	2 cut(s)	1024, 1482
AccBSI	CCGCTC	1 cut(s)	67
AccI	GTMKAC	3 cut(s)	212, 327, 2084
AccIII	TCCGGA	1 cut(s)	835
AciI	CCGC	8 cut(s)	23, 60, 65, 396, 937, 949, 982, 2372
AclWI	GGATC	2 cut(s)	234, 2126
AcoI	YGGCCR	2 cut(s)	407, 1228
AcsI	RAATTY	3 cut(s)	584, 597, 732
AcuI	CTGAAG	2 cut(s)	696, 2252
AcvI	CACGTG	1 cut(s)	284
AcyI	GRCGYC	2 cut(s)	215, 1784
AfaI	GTAC	4 cut(s)	812, 1409, 1436, 1670
AfiI	CCNNNNNNNGG	6 cut(s)	28, 1024, 1226, 1278, 1477, 1482
AflII	CTTAAG	2 cut(s)	362, 703
AflIII	ACRYGT	1 cut(s)	281
AgeI	ACCGGT	1 cut(s)	1096
AjnI	CCWGG	4 cut(s)	620, 858, 1175, 1735
AjuI	GAANNNNNNNTTGG	2 cut(s)	835, 867
Alw21I	GWGCWC	1 cut(s)	1924
Alw26I	GTCTC	4 cut(s)	1055, 1734, 1791, 2468
Alw44I	GTGCAC	1 cut(s)	1920
AlwI	GGATC	2 cut(s)	234, 2126
Ama87I	CYCGRG	2 cut(s)	68, 1606
Aor13HI	TCCGGA	1 cut(s)	835
AoxI	GGCC	5 cut(s)	61, 332, 407, 1228, 1843
ApaLI	GTGCAC	1 cut(s)	1920
ApeKI	GCWGC	3 cut(s)	25, 78, 758
ApoI	RAATTY	3 cut(s)	584, 597, 732
ArsI	GACNNNNNNTTYG	2 cut(s)	158, 190
AseI	ATTAAT	2 cut(s)	1304, 1991
AsiGI	ACCGGT	1 cut(s)	1096
Asp700I	GAANNNNTTC	1 cut(s)	1953
AspLEI	GCGC	2 cut(s)	1084, 2417
AspS9I	GGNCC	6 cut(s)	62, 72, 333, 838, 1281, 1910
AsuC2I	CCSGG	4 cut(s)	345, 1285, 1607, 1608
AsuHPI	GGTGA	5 cut(s)	410, 697, 1409, 1787, 2332
AvaI	CYCGRG	2 cut(s)	68, 1606
AvaII	GGWCC	4 cut(s)	72, 838, 1281, 1910
BaeGI	GKGCMC	2 cut(s)	361, 1924
BalI	TGGCCA	1 cut(s)	409
BanI	GGYRCC	1 cut(s)	942
BarI	GAAGNNNNNNTAC	2 cut(s)	1941, 1973
BauI	CACGAG	1 cut(s)	354
BbrPI	CACGTG	1 cut(s)	284
BbsI	GAAGAC	4 cut(s)	129, 241, 1006, 2330
Bbv12I	GWGCWC	1 cut(s)	1924
BbvCI	CCTCAGC	1 cut(s)	747
BbvI	GCAGC	3 cut(s)	12, 90, 770
BccI	CCATC	6 cut(s)	149, 440, 656, 1164, 1422, 1901
BceAI	ACGGC	1 cut(s)	2206
BcgI	CGANNNNNNTGC	2 cut(s)	31, 65
BciT130I	CCWGG	4 cut(s)	622, 860, 1177, 1737
BciVI	GTATCC	1 cut(s)	1451
BclI	TGATCA	3 cut(s)	781, 1198, 2386
BcnI	CCSGG	4 cut(s)	345, 1285, 1607, 1608
BcoDI	GTCTC	4 cut(s)	1055, 1734, 1791, 2468
BfaI	CTAG	5 cut(s)	227, 737, 1832, 2042, 2244
BfoI	RGCGCY	1 cut(s)	1085
BfrI	CTTAAG	2 cut(s)	362, 703
BfuI	GTATCC	1 cut(s)	1451
BglII	AGATCT	2 cut(s)	1584, 2246
BisI	GCNGC	5 cut(s)	26, 61, 79, 759, 950
BlsI	GCNGC	5 cut(s)	27, 62, 80, 760, 951
Bme1390I	CCNGG	8 cut(s)	345, 622, 860, 1177, 1285, 1607, 1608, 1737
Bme18I	GGWCC	4 cut(s)	72, 838, 1281, 1910
BmeT110I	CYCGRG	2 cut(s)	68, 1606
BmgT120I	GGNCC	6 cut(s)	62, 72, 333, 838, 1281, 1910
BmiI	GGNNCC	8 cut(s)	73, 840, 909, 944, 1153, 1270, 1340, 1734
BmrFI	CCNGG	8 cut(s)	345, 622, 860, 1177, 1285, 1607, 1608, 1737
BmrI	ACTGGG	3 cut(s)	1398, 1492, 1514
BmsI	GCATC	5 cut(s)	514, 1720, 1878, 2230, 2389
BmuI	ACTGGG	3 cut(s)	1398, 1492, 1514
BpiI	GAAGAC	4 cut(s)	129, 241, 1006, 2330
BplI	GAGNNNNNCTC	2 cut(s)	2120, 2152
BpmI	CTGGAG	2 cut(s)	997, 1758
Bpu10I	CCTNAGC	1 cut(s)	747
BpuEI	CTTGAG	5 cut(s)	254, 331, 356, 1052, 1914
BpuMI	CCSGG	4 cut(s)	345, 1285, 1607, 1608
BsaAI	YACGTR	1 cut(s)	284
BsaBI	GATNNNNATC	1 cut(s)	1573
BsaHI	GRCGYC	2 cut(s)	215, 1784
BsaI	GGTCTC	2 cut(s)	1055, 2468
BsaJI	CCNNGG	9 cut(s)	567, 793, 1175, 1272, 1284, 1334, 1453, 1476, 1606
BsaWI	WCCGGW	2 cut(s)	835, 1096
BsaXI	ACNNNNNCTCC	2 cut(s)	1717, 1747
Bsc4I	CCNNNNNNNGG	6 cut(s)	28, 1024, 1226, 1278, 1477, 1482
Bse118I	RCCGGY	3 cut(s)	885, 945, 1096
Bse1I	ACTGG	8 cut(s)	980, 1256, 1393, 1487, 1520, 1659, 2120, 2220
Bse3DI	GCAATG	1 cut(s)	1041
Bse8I	GATNNNNATC	1 cut(s)	1573
BseAI	TCCGGA	1 cut(s)	835
BseBI	CCWGG	4 cut(s)	622, 860, 1177, 1737
BseDI	CCNNGG	9 cut(s)	567, 793, 1175, 1272, 1284, 1334, 1453, 1476, 1606
BseGI	GGATG	7 cut(s)	492, 1437, 1509, 1711, 1893, 2305, 2380
BseJI	GATNNNNATC	1 cut(s)	1573
BseLI	CCNNNNNNNGG	6 cut(s)	28, 1024, 1226, 1278, 1477, 1482
BseMI	GCAATG	1 cut(s)	1041
BseMII	CTCAG	7 cut(s)	198, 738, 1050, 1802, 1823, 1897, 2463
BseNI	ACTGG	8 cut(s)	980, 1256, 1393, 1487, 1520, 1659, 2120, 2220
BseRI	GAGGAG	3 cut(s)	103, 210, 1818
BseSI	GKGCMC	2 cut(s)	361, 1924
BseXI	GCAGC	3 cut(s)	12, 90, 770
BsgI	GTGCAG	3 cut(s)	1123, 2189, 2200
BshFI	GGCC	5 cut(s)	63, 334, 409, 1230, 1845
BshNI	GGYRCC	1 cut(s)	942
BshTI	ACCGGT	1 cut(s)	1096
BsiHKAI	GWGCWC	1 cut(s)	1924
BsiHKCI	CYCGRG	2 cut(s)	68, 1606
BsiSI	CCGG	8 cut(s)	29, 345, 836, 886, 946, 1097, 1284, 1607
BslFI	GGGAC	3 cut(s)	85, 381, 1499
BslI	CCNNNNNNNGG	6 cut(s)	28, 1024, 1226, 1278, 1477, 1482
BsmAI	GTCTC	4 cut(s)	1055, 1734, 1791, 2468
BsmBI	CGTCTC	2 cut(s)	1734, 1791
BsmFI	GGGAC	3 cut(s)	85, 381, 1499
BsnI	GGCC	5 cut(s)	63, 334, 409, 1230, 1845
Bso31I	GGTCTC	2 cut(s)	1055, 2468
BsoBI	CYCGRG	2 cut(s)	68, 1606
Bsp1286I	GDGCHC	2 cut(s)	361, 1924
Bsp13I	TCCGGA	1 cut(s)	835
Bsp1407I	TGTACA	2 cut(s)	810, 1434
Bsp19I	CCATGG	1 cut(s)	1453
BspACI	CCGC	8 cut(s)	23, 60, 65, 396, 937, 949, 982, 2372
BspANI	GGCC	5 cut(s)	63, 334, 409, 1230, 1845
BspCNI	CTCAG	7 cut(s)	199, 739, 1051, 1801, 1822, 1896, 2464
BspEI	TCCGGA	1 cut(s)	835
BspHI	TCATGA	2 cut(s)	378, 784
BspLI	GGNNCC	8 cut(s)	73, 840, 909, 944, 1153, 1270, 1340, 1734
BspPI	GGATC	2 cut(s)	234, 2126
BspQI	GCTCTTC	1 cut(s)	768
BspT107I	GGYRCC	1 cut(s)	942
BspTI	CTTAAG	2 cut(s)	362, 703
BspTNI	GGTCTC	2 cut(s)	1055, 2468
BsrBI	CCGCTC	1 cut(s)	67
BsrDI	GCAATG	1 cut(s)	1041
BsrFI	RCCGGY	3 cut(s)	885, 945, 1096
BsrGI	TGTACA	2 cut(s)	810, 1434
BsrI	ACTGG	8 cut(s)	980, 1256, 1393, 1487, 1520, 1659, 2120, 2220
BssAI	RCCGGY	3 cut(s)	885, 945, 1096
BssECI	CCNNGG	9 cut(s)	567, 793, 1175, 1272, 1284, 1334, 1453, 1476, 1606
BssNI	GRCGYC	2 cut(s)	215, 1784
BssSI	CACGAG	1 cut(s)	354
BssT1I	CCWWGG	4 cut(s)	567, 1334, 1453, 1476
Bst2BI	CACGAG	1 cut(s)	354
Bst2UI	CCWGG	4 cut(s)	622, 860, 1177, 1737
Bst4CI	ACNGT	3 cut(s)	1600, 1668, 1841
Bst6I	CTCTTC	5 cut(s)	459, 567, 675, 768, 2069
BstACI	GRCGYC	2 cut(s)	215, 1784
BstAFI	CTTAAG	2 cut(s)	362, 703
BstAUI	TGTACA	2 cut(s)	810, 1434
BstBAI	YACGTR	1 cut(s)	284
BstC8I	GCNNGC	4 cut(s)	65, 511, 744, 947
BstDSI	CCRYGG	1 cut(s)	1453
BstENI	CCTNNNNNAGG	1 cut(s)	1475
BstF5I	GGATG	7 cut(s)	492, 1437, 1509, 1711, 1893, 2305, 2380
BstH2I	RGCGCY	1 cut(s)	1085
BstHHI	GCGC	2 cut(s)	1084, 2417
BstMAI	GTCTC	4 cut(s)	1055, 1734, 1791, 2468
BstMWI	GCNNNNNNNGC	3 cut(s)	105, 340, 2249
BstNI	CCWGG	4 cut(s)	622, 860, 1177, 1737
BstNSI	RCATGY	4 cut(s)	746, 1167, 1539, 2369
BstSCI	CCNGG	8 cut(s)	343, 620, 858, 1175, 1283, 1605, 1606, 1735
BstSLI	GKGCMC	2 cut(s)	361, 1924
BstV1I	GCAGC	3 cut(s)	12, 90, 770
BstV2I	GAAGAC	4 cut(s)	129, 241, 1006, 2330
BstX2I	RGATCY	2 cut(s)	1584, 2246
BstYI	RGATCY	2 cut(s)	1584, 2246
BsuI	GTATCC	1 cut(s)	1451
BsuRI	GGCC	5 cut(s)	63, 334, 409, 1230, 1845
BtgI	CCRYGG	1 cut(s)	1453
BtsCI	GGATG	7 cut(s)	492, 1437, 1509, 1711, 1893, 2305, 2380
BtsIMutI	CAGTG	4 cut(s)	1584, 1666, 1846, 2227
Cac8I	GCNNGC	4 cut(s)	65, 511, 744, 947
CciI	TCATGA	2 cut(s)	378, 784
CfoI	GCGC	2 cut(s)	1084, 2417
Cfr10I	RCCGGY	3 cut(s)	885, 945, 1096
Cfr13I	GGNCC	6 cut(s)	62, 72, 333, 838, 1281, 1910
Cfr9I	CCCGGG	1 cut(s)	1606
CseI	GACGC	1 cut(s)	2335
Csp6I	GTAC	4 cut(s)	811, 1408, 1435, 1669
CspAI	ACCGGT	1 cut(s)	1096
CviQI	GTAC	4 cut(s)	811, 1408, 1435, 1669
DraI	TTTAAA	1 cut(s)	265
EaeI	YGGCCR	2 cut(s)	407, 1228
Eam1104I	CTCTTC	5 cut(s)	459, 567, 675, 768, 2069
EarI	CTCTTC	5 cut(s)	459, 567, 675, 768, 2069
EciI	GGCGGA	1 cut(s)	997
Eco130I	CCWWGG	4 cut(s)	567, 1334, 1453, 1476
Eco31I	GGTCTC	2 cut(s)	1055, 2468
Eco47I	GGWCC	4 cut(s)	72, 838, 1281, 1910
Eco57I	CTGAAG	2 cut(s)	696, 2252
Eco72I	CACGTG	1 cut(s)	284
Eco88I	CYCGRG	2 cut(s)	68, 1606
EcoNI	CCTNNNNNAGG	1 cut(s)	1475
EcoRII	CCWGG	4 cut(s)	620, 858, 1175, 1735
EcoT14I	CCWWGG	4 cut(s)	567, 1334, 1453, 1476
EcoT22I	ATGCAT	3 cut(s)	482, 529, 1537
ErhI	CCWWGG	4 cut(s)	567, 1334, 1453, 1476
Esp3I	CGTCTC	2 cut(s)	1734, 1791
FalI	AAGNNNNNCTT	2 cut(s)	1998, 2030
FaqI	GGGAC	3 cut(s)	85, 381, 1499
FauI	CCCGC	2 cut(s)	30, 72
FauNDI	CATATG	2 cut(s)	1629, 1915
FbaI	TGATCA	3 cut(s)	781, 1198, 2386
FblI	GTMKAC	3 cut(s)	212, 327, 2084
Fnu4HI	GCNGC	5 cut(s)	26, 61, 79, 759, 950
FokI	GGATG	7 cut(s)	499, 1424, 1516, 1698, 1900, 2312, 2367
Fsp4HI	GCNGC	5 cut(s)	26, 61, 79, 759, 950
FspBI	CTAG	5 cut(s)	227, 737, 1832, 2042, 2244
GlaI	GCGC	2 cut(s)	1083, 2416
GluI	GCNGC	5 cut(s)	26, 61, 79, 759, 950
GsuI	CTGGAG	2 cut(s)	997, 1758
HaeII	RGCGCY	1 cut(s)	1085
HaeIII	GGCC	5 cut(s)	63, 334, 409, 1230, 1845
HapII	CCGG	8 cut(s)	29, 345, 836, 886, 946, 1097, 1284, 1607
HgaI	GACGC	1 cut(s)	2335
HhaI	GCGC	2 cut(s)	1084, 2417
Hin1I	GRCGYC	2 cut(s)	215, 1784
Hin6I	GCGC	2 cut(s)	1082, 2415
HinP1I	GCGC	2 cut(s)	1082, 2415
HincII	GTYRAC	2 cut(s)	213, 328
HindII	GTYRAC	2 cut(s)	213, 328
HinfI	GANTC	6 cut(s)	7, 209, 369, 494, 669, 1574
HpaII	CCGG	8 cut(s)	29, 345, 836, 886, 946, 1097, 1284, 1607
HphI	GGTGA	5 cut(s)	410, 697, 1409, 1787, 2332
Hpy166II	GTNNAC	8 cut(s)	213, 287, 328, 1435, 1603, 1664, 1922, 2085
Hpy188I	TCNGA	9 cut(s)	186, 194, 540, 604, 676, 797, 882, 912, 2256
Hpy8I	GTNNAC	8 cut(s)	213, 287, 328, 1435, 1603, 1664, 1922, 2085
Hpy99I	CGWCG	5 cut(s)	167, 217, 540, 1347, 2192
HpyAV	CCTTC	6 cut(s)	316, 395, 643, 1258, 1856, 2228
HpyCH4III	ACNGT	3 cut(s)	1600, 1668, 1841
HpyCH4IV	ACGT	6 cut(s)	215, 283, 1614, 1769, 1784, 1822
HpyF10VI	GCNNNNNNNGC	3 cut(s)	105, 340, 2249
HpySE526I	ACGT	6 cut(s)	215, 283, 1614, 1769, 1784, 1822
Hsp92I	GRCGYC	2 cut(s)	215, 1784
HspAI	GCGC	2 cut(s)	1082, 2415
Kpn2I	TCCGGA	1 cut(s)	835
KroI	GCCGGC	1 cut(s)	945
KroNI	GCCGGC	1 cut(s)	947
Ksp22I	TGATCA	3 cut(s)	781, 1198, 2386
LguI	GCTCTTC	1 cut(s)	768
LmnI	GCTCC	5 cut(s)	90, 197, 1055, 1738, 1979
Lsp1109I	GCAGC	3 cut(s)	12, 90, 770
LweI	GCATC	5 cut(s)	514, 1720, 1878, 2230, 2389
MaeI	CTAG	5 cut(s)	227, 737, 1832, 2042, 2244
MaeII	ACGT	6 cut(s)	215, 283, 1614, 1769, 1784, 1822
MaeIII	GTNAC	4 cut(s)	277, 562, 1384, 1594
MbiI	CCGCTC	1 cut(s)	67
MflI	RGATCY	2 cut(s)	1584, 2246
MhlI	GDGCHC	2 cut(s)	361, 1924
MlsI	TGGCCA	1 cut(s)	409
MluCI	AATT	9 cut(s)	434, 584, 597, 732, 1086, 1113, 1681, 1722, 1974
MluNI	TGGCCA	1 cut(s)	409
MlyI	GAGTC	3 cut(s)	218, 363, 503
MmeI	TCCRAC	4 cut(s)	518, 935, 1945, 2377
Mox20I	TGGCCA	1 cut(s)	409
Mph1103I	ATGCAT	3 cut(s)	482, 529, 1537
MroI	TCCGGA	1 cut(s)	835
MroNI	GCCGGC	1 cut(s)	945
MroXI	GAANNNNTTC	1 cut(s)	1953
MscI	TGGCCA	1 cut(s)	409
MslI	CAYNNNNRTG	2 cut(s)	485, 1705
Msp20I	TGGCCA	1 cut(s)	409
MspA1I	CMGCKG	1 cut(s)	25
MspCI	CTTAAG	2 cut(s)	362, 703
MspI	CCGG	8 cut(s)	29, 345, 836, 886, 946, 1097, 1284, 1607
MspR9I	CCNGG	8 cut(s)	345, 622, 860, 1177, 1285, 1607, 1608, 1737
MvaI	CCWGG	4 cut(s)	622, 860, 1177, 1737
MwoI	GCNNNNNNNGC	3 cut(s)	105, 340, 2249
NaeI	GCCGGC	1 cut(s)	947
NciI	CCSGG	4 cut(s)	345, 1285, 1607, 1608
NcoI	CCATGG	1 cut(s)	1453
NdeI	CATATG	2 cut(s)	1629, 1915
NgoMIV	GCCGGC	1 cut(s)	945
NlaIV	GGNNCC	8 cut(s)	73, 840, 909, 944, 1153, 1270, 1340, 1734
NmuCI	GTSAC	2 cut(s)	277, 562
NsiI	ATGCAT	3 cut(s)	482, 529, 1537
NspI	RCATGY	4 cut(s)	746, 1167, 1539, 2369
PaeI	GCATGC	1 cut(s)	746
PagI	TCATGA	2 cut(s)	378, 784
PciSI	GCTCTTC	1 cut(s)	768
PcsI	WCGNNNNNNNCGW	1 cut(s)	159
PdiI	GCCGGC	1 cut(s)	947
PdmI	GAANNNNTTC	1 cut(s)	1953
PfeI	GAWTC	3 cut(s)	7, 669, 1574
PflMI	CCANNNNNTGG	2 cut(s)	1024, 1482
PfoI	TCCNGGA	1 cut(s)	1735
PinAI	ACCGGT	1 cut(s)	1096
PkrI	GCNGC	5 cut(s)	27, 62, 80, 760, 951
PleI	GAGTC	3 cut(s)	217, 363, 502
PmaCI	CACGTG	1 cut(s)	284
PmlI	CACGTG	1 cut(s)	284
PpsI	GAGTC	3 cut(s)	217, 363, 502
Ppu21I	YACGTR	1 cut(s)	284
PshBI	ATTAAT	2 cut(s)	1304, 1991
Psp6I	CCWGG	4 cut(s)	620, 858, 1175, 1735
PspCI	CACGTG	1 cut(s)	284
PspGI	CCWGG	4 cut(s)	620, 858, 1175, 1735
PspN4I	GGNNCC	8 cut(s)	73, 840, 909, 944, 1153, 1270, 1340, 1734
PspPI	GGNCC	6 cut(s)	62, 72, 333, 838, 1281, 1910
PsuI	RGATCY	2 cut(s)	1584, 2246
RsaI	GTAC	4 cut(s)	812, 1409, 1436, 1670
RsaNI	GTAC	4 cut(s)	811, 1408, 1435, 1669
RseI	CAYNNNNRTG	2 cut(s)	485, 1705
SalI	GTCGAC	2 cut(s)	211, 326
SapI	GCTCTTC	1 cut(s)	768
SatI	GCNGC	5 cut(s)	26, 61, 79, 759, 950
Sau96I	GGNCC	6 cut(s)	62, 72, 333, 838, 1281, 1910
SchI	GAGTC	3 cut(s)	218, 363, 503
ScrFI	CCNGG	8 cut(s)	345, 622, 860, 1177, 1285, 1607, 1608, 1737
SduI	GDGCHC	2 cut(s)	361, 1924
SfaNI	GCATC	5 cut(s)	514, 1720, 1878, 2230, 2389
SinI	GGWCC	4 cut(s)	72, 838, 1281, 1910
SmaI	CCCGGG	1 cut(s)	1608
SmiMI	CAYNNNNRTG	2 cut(s)	485, 1705
SmlI	CTYRAG	7 cut(s)	269, 310, 362, 371, 703, 1067, 1929
SmoI	CTYRAG	7 cut(s)	269, 310, 362, 371, 703, 1067, 1929
SphI	GCATGC	1 cut(s)	746
Sse9I	AATT	9 cut(s)	434, 584, 597, 732, 1086, 1113, 1681, 1722, 1974
SsiI	CCGC	8 cut(s)	23, 60, 65, 396, 937, 949, 982, 2372
SspMI	CTAG	5 cut(s)	227, 737, 1832, 2042, 2244
StyD4I	CCNGG	8 cut(s)	343, 620, 858, 1175, 1283, 1605, 1606, 1735
StyI	CCWWGG	4 cut(s)	567, 1334, 1453, 1476
TaaI	ACNGT	3 cut(s)	1600, 1668, 1841
TaiI	ACGT	6 cut(s)	218, 286, 1617, 1772, 1787, 1825
TaqII	GACCGA	1 cut(s)	2492
TasI	AATT	9 cut(s)	434, 584, 597, 732, 1086, 1113, 1681, 1722, 1974
TatI	WGTACW	3 cut(s)	810, 1434, 1668
TauI	GCSGC	2 cut(s)	63, 952
TfiI	GAWTC	3 cut(s)	7, 669, 1574
TscAI	CASTG	4 cut(s)	1584, 1666, 1846, 2227
TseFI	GTSAC	2 cut(s)	277, 562
TseI	GCWGC	3 cut(s)	25, 78, 758
Tsp45I	GTSAC	2 cut(s)	277, 562
TspDTI	ATGAA	9 cut(s)	314, 447, 503, 1229, 1277, 1587, 1748, 2177, 2244
TspGWI	ACGGA	2 cut(s)	232, 1331
TspMI	CCCGGG	1 cut(s)	1606
TspRI	CASTG	4 cut(s)	1584, 1666, 1846, 2227
Van91I	CCANNNNNTGG	2 cut(s)	1024, 1482
Vha464I	CTTAAG	2 cut(s)	362, 703
VneI	GTGCAC	1 cut(s)	1920
VpaK11BI	GGWCC	4 cut(s)	72, 838, 1281, 1910
VspI	ATTAAT	2 cut(s)	1304, 1991
XagI	CCTNNNNNAGG	1 cut(s)	1475
XapI	RAATTY	3 cut(s)	584, 597, 732
XbaI	TCTAGA	2 cut(s)	736, 1831
XceI	RCATGY	4 cut(s)	746, 1167, 1539, 2369
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2288, 2293
XmaI	CCCGGG	1 cut(s)	1606
XmiI	GTMKAC	3 cut(s)	212, 327, 2084
XmnI	GAANNNNTTC	1 cut(s)	1953
XspI	CTAG	5 cut(s)	227, 737, 1832, 2042, 2244
ZraI	GACGTC	2 cut(s)	216, 1785
Zsp2I	ATGCAT	3 cut(s)	482, 529, 1537

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.