Rh3DG070800

Respiratory burst

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0005589
Physical Location & Seq
Forward (+)
4797914 .. 4802836
4923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG070800.1

Sequence Viewer

Length: 2625 bp
ATGGAGATTCAAGCAGAAAACCCGCTGCCGGATAAAGAGAGCAAACGGGTCGGGCGGGGCAGCCCGCTAGGGACCAGCAGCAAGATGAGGAGCAACAGAAGCACAATGTTCAAAGATGACGATCAATGCTATGTGGAAGTGGCACTCGATGTTTGCGACAACAATACCTTTGGGCTTCAGAACATCAGAGGAGCTGAACCTGAGTCAACGTCAGCAAGGCTAGAGAGAAGACTTGCTTCTCAACTCTCTTTTAAACTCAAGAAAGTCTCACGTGAACTGAAACGCATGAAATCTTCCTTGAGCTTCAAGAAGGTCGACAGACCCACCAAGCCCGGTATTGCTCGTGCCCTTAAGGGACTCAAGCTCATGACCAAAAATGTGTGCAGTGAAGGTTGGCCAGAGATAGAAATAAGGTTTGATGAATTGGCTGTCGATGGAACTCTTCCAAAATCAGTCTTTGCTCAATGCATAGGGATGAAAGAGTCGAGTGAGTTTGCTTGCGAAGTATTCGATGCATTGGCTCGTCGGAGAGGAATAACATCGTGTTGTGTGACGAAGGAAGAGTTGAGAGAATTTTGGGAACAAATTTCAGATCACAGCTTCAATGCCAGGCTTCAAACCTTCTTTGACATGGTGGATAAAAATGCAGATGGTCGAATCTCTGAAGAAGAGGTGAAAGAGATTATTACCTTAAGTGCCTCTGCTAATAAGCTGTCAAAAATTCTAGAACATGCTGAGGAATATGCAGCATTGATTATGGAAGAGCTTGATCATGACAACCTTGGATATATTGATTTGTACAACTTGAAAATGCTACTTCTTCCGGGCCCAACCCAATCTTCGAACCTGGAAACAGACAGCTACATTCAGAGCCGGTTGCTAAGTGAAAAGCTGGTTCCGACCAAAGAGCACAACCCTATAAGACGGTGGTGCCGGCGGCTGTCCTACTTTGTTGAGGATAATTGGAGGCGGATATGGGTTGTTCTGCTATGGCTTTTTATATGCATGGGCCTCTTTGCTTGGAAATTCATTCAGTATAGACATAGGGAGGTCTTCCATGTAATGGGCTATTGTGTGTGCATTGCCAAAGGTGGAGCTGAGACCCTAAAGTTTAACATGGCCCTGATTCTTCTTCCGGTGTGTAGAAATACAATTACTTGGCTAAGGAGCAAGACCAAGTTAGGAGGTGTAGTTCCATTTGATGACAATATCAATTTTCATAAGGTGATCGCGTTTGGAATTGCAGTTGGGGTCAGTCTCCATGCCGGTTCCCATCTTACATGCGACTTCCCCAGGCTACTACACTCAACCAATGATCAATACGACAAGATGAAGCCATTTTTCGGCCAAAAACGACCCGACAACTACTGGTGGTTTTTGAAGGGAACCGAGGGCTGGACCGGGGTGGTTATGGTGGCACTAATGCTAGTTGCATACACATTGGCTCAACCTTGGTTCCGTCGCAATCGACTAAGAAACCTCCCTAAAACCTTAAAGAAAATTACAGGGTTCAATGCATTTTGGTACTCCCATCACCTCTTTATCATTGTGTACATCCTCCTTATTATCCATGGATACTTCCTGTACCTCTCCAAGGACTGGGACAAGAAAACAACATGGATGTATCTGGCTTTCCCAGTAATAATATATGCATGTGAGCGTTTGCTTCGTGCTTTCAGGTCTGGATATGAATCAGTGAAGATCTTAAAGGTTACTGTTTATCCGGGAAACGTATTGGCACTCCATATGTCTAAACCACAGGGATTAAAATATACCAGTGGACAGTACATTTACATAAATTGTCCTACTATTTCCTCATTTCAATGGCATCCATTCTCAATTACTTCAGCTCCTGGAGACGACTATCTAAGTGTTCATATTCGCACGTTGGGTGATTGGACATCTCAGCTCAAATACATTTTCTCTCAGGTTTGCCAACGTCCTCCTCTAGAGCTCAGTGGCCTTCTAAGAGCTGATACCGAACAACCCGATATACAACTCAGGATGCCAAGACTTCTTATTGATGGTCCATATGGTGCACCAGCTCAAGGCTACAAAAACTACGAAGTTCTTCTACTTGTTGGACTTGGAATTGGAGCAACTCCATTAATAAGCATACTCAAAGATGTGCTTAACAACATTAAGCGACAGAAAGAACTAGAAGCTCAAATGTTGGTGGAAAGTGGTGTTAAGAGAAAGTCTACTTTTGCAACAAAACGAGCTTATTTCTACTGGATCACAAGAGAGCAAGGCTCGTTCGAGTGGTTTAGGGGTGTGATGAATGAGGTCGCAGAAAATGACCTCGACGGCGTGATTGAGCTGCACAACTACTGCACCAGTGTCTATGAAGAAGGAGATGCTAGATCTGCTCTGATTACTATGCTTCAGTCTATAAACCATGCCAAAAATGGTGTGGATGTTGTATCAGGAACGAGGGTGAAGACGCATTTTGCAAGACCAAAATGGCGTAATGTTTTTAAGCATGTGGCAGTAAAGCATCCTGATAAAAAAGTTGGAGTGTTTTATTGTGGCGCACATGGATTAGTTGGGGAGTTAAAGAGGTTAGCTAAAGACTTTTCCAGAAAAACTGAGACCGAGTTTGATTTCCACAAAGAGAACTTTTAG

Protein Analysis

874

Amino Acids

100.52

Weight (kDa)

9.17

Isoelectric Point (pI)

42.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADPH_Ox PF08414 109 - 203 2e-32 Respiratory burst NADPH oxidase
EF-hand_7 PF13499 204 - 266 6.4e-06 EF-hand domain pair
Ferric_reduct PF01794 372 - 522 9.3e-12 Ferric reductase like transmembrane component
FAD_binding_8 PF08022 565 - 682 2.4e-35 FAD-binding domain
NAD_binding_6 PF08030 688 - 856 4.1e-50 Ferric reductase NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 930
AccB7I CCANNNNNTGG 2 cut(s) 1063, 1599
AccI GTMKAC 2 cut(s) 315, 2201
AccII CGCG 1 cut(s) 1232
AciI CCGC 5 cut(s) 23, 55, 65, 937, 970
AclWI GGATC 1 cut(s) 2243
AcoI YGGCCR 2 cut(s) 395, 1345
AcsI RAATTY 4 cut(s) 572, 585, 720, 1025
AcuI CTGAAG 4 cut(s) 161, 684, 1830, 2369
AcvI CACGTG 1 cut(s) 272
AfaI GTAC 5 cut(s) 800, 1526, 1553, 1586, 1787
AfiI CCNNNNNNNGG 7 cut(s) 28, 1063, 1343, 1395, 1594, 1599, 2048
AflII CTTAAG 2 cut(s) 350, 691
AgsI TTSAA 9 cut(s) 11, 112, 307, 604, 617, 808, 1381, 1513, 1823
AjnI CCWGG 4 cut(s) 608, 846, 1292, 1852
AjuI GAANNNNNNNTTGG 2 cut(s) 823, 855
AloI GAACNNNNNNTCC 2 cut(s) 1176, 1208
Alw21I GWGCWC 3 cut(s) 912, 1956, 2041
Alw26I GTCTC 5 cut(s) 271, 1094, 1262, 1851, 2585
Alw44I GTGCAC 1 cut(s) 2037
AlwI GGATC 1 cut(s) 2243
AlwNI CAGNNNCTG 1 cut(s) 1853
AoxI GGCC 6 cut(s) 395, 826, 1009, 1119, 1345, 1960
ApaI GGGCCC 1 cut(s) 830
ApaLI GTGCAC 1 cut(s) 2037
ApeKI GCWGC 5 cut(s) 25, 60, 78, 746, 2320
ApoI RAATTY 4 cut(s) 572, 585, 720, 1025
AseI ATTAAT 1 cut(s) 2108
Asp700I GAANNNNTTC 1 cut(s) 2070
AspLEI GCGC 1 cut(s) 2534
AspS9I GGNCC 7 cut(s) 72, 826, 827, 1009, 1120, 1398, 2027
AsuC2I CCSGG 4 cut(s) 333, 825, 1402, 1725
AsuHPI GGTGA 5 cut(s) 685, 1237, 1526, 1904, 2449
AsuII TTCGAA 1 cut(s) 842
AvaII GGWCC 3 cut(s) 72, 1398, 2027
BaeGI GKGCMC 3 cut(s) 349, 830, 2041
BalI TGGCCA 1 cut(s) 397
BanI GGYRCC 1 cut(s) 930
BanII GRGCYC 2 cut(s) 830, 1956
BarI GAAGNNNNNNTAC 2 cut(s) 2058, 2090
BauI CACGAG 1 cut(s) 342
BbrPI CACGTG 1 cut(s) 272
BbsI GAAGAC 3 cut(s) 235, 1045, 2447
Bbv12I GWGCWC 3 cut(s) 912, 1956, 2041
BbvCI CCTCAGC 1 cut(s) 735
BbvI GCAGC 5 cut(s) 12, 72, 90, 758, 2307
BccI CCATC 5 cut(s) 428, 644, 1281, 1539, 2018
BceAI ACGGC 1 cut(s) 2323
BcgI CGANNNNNNTGC 2 cut(s) 31, 65
BciT130I CCWGG 4 cut(s) 610, 848, 1294, 1854
BciVI GTATCC 1 cut(s) 1568
BclI TGATCA 2 cut(s) 769, 1315
BcnI CCSGG 4 cut(s) 333, 825, 1402, 1725
BcoDI GTCTC 5 cut(s) 271, 1094, 1262, 1851, 2585
BfaI CTAG 7 cut(s) 68, 221, 725, 1427, 1949, 2159, 2361
BfrI CTTAAG 2 cut(s) 350, 691
BfuI GTATCC 1 cut(s) 1568
BglII AGATCT 2 cut(s) 1701, 2363
BisI GCNGC 6 cut(s) 26, 61, 79, 747, 938, 2321
BlsI GCNGC 6 cut(s) 27, 62, 80, 748, 939, 2322
Bme1390I CCNGG 8 cut(s) 333, 610, 825, 848, 1294, 1402, 1725, 1854
Bme18I GGWCC 3 cut(s) 72, 1398, 2027
BmgT120I GGNCC 7 cut(s) 72, 826, 827, 1009, 1120, 1398, 2027
BmiI GGNNCC 7 cut(s) 73, 828, 897, 932, 1270, 1387, 1457
BmrFI CCNGG 8 cut(s) 333, 610, 825, 848, 1294, 1402, 1725, 1854
BmrI ACTGGG 2 cut(s) 1609, 1631
BmsI GCATC 5 cut(s) 502, 1837, 1995, 2347, 2506
BmuI ACTGGG 2 cut(s) 1609, 1631
BpiI GAAGAC 3 cut(s) 235, 1045, 2447
BplI GAGNNNNNCTC 2 cut(s) 2237, 2269
BpmI CTGGAG 1 cut(s) 1875
Bpu10I CCTNAGC 2 cut(s) 735, 1163
Bpu14I TTCGAA 1 cut(s) 842
BpuEI CTTGAG 4 cut(s) 242, 319, 344, 2031
BpuMI CCSGG 4 cut(s) 333, 825, 1402, 1725
BsaAI YACGTR 1 cut(s) 272
BsaBI GATNNNNATC 2 cut(s) 120, 1690
BsaI GGTCTC 2 cut(s) 1094, 2585
BsaJI CCNNGG 7 cut(s) 781, 1292, 1389, 1401, 1451, 1570, 1593
BsaWI WCCGGW 1 cut(s) 1135
BsaXI ACNNNNNCTCC 4 cut(s) 1176, 1206, 1834, 1864
Bsc4I CCNNNNNNNGG 7 cut(s) 28, 1063, 1343, 1395, 1594, 1599, 2048
Bse118I RCCGGY 3 cut(s) 873, 933, 1265
Bse1I ACTGG 6 cut(s) 1373, 1604, 1637, 1776, 2237, 2337
Bse3DI GCAATG 1 cut(s) 1080
Bse8I GATNNNNATC 2 cut(s) 120, 1690
BseBI CCWGG 4 cut(s) 610, 848, 1294, 1854
BseDI CCNNGG 7 cut(s) 781, 1292, 1389, 1401, 1451, 1570, 1593
BseGI GGATG 7 cut(s) 480, 1554, 1626, 1828, 2010, 2422, 2497
BseJI GATNNNNATC 2 cut(s) 120, 1690
BseLI CCNNNNNNNGG 7 cut(s) 28, 1063, 1343, 1395, 1594, 1599, 2048
BseMI GCAATG 1 cut(s) 1080
BseMII CTCAG 8 cut(s) 192, 726, 1089, 1919, 1940, 1969, 2014, 2580
BseNI ACTGG 6 cut(s) 1373, 1604, 1637, 1776, 2237, 2337
BseRI GAGGAG 3 cut(s) 103, 204, 1935
BseSI GKGCMC 3 cut(s) 349, 830, 2041
BseXI GCAGC 5 cut(s) 12, 72, 90, 758, 2307
BsgI GTGCAG 3 cut(s) 403, 2306, 2317
Bsh1236I CGCG 1 cut(s) 1232
BshFI GGCC 6 cut(s) 397, 828, 1011, 1121, 1347, 1962
BshNI GGYRCC 1 cut(s) 930
BsiHKAI GWGCWC 3 cut(s) 912, 1956, 2041
BsiSI CCGG 9 cut(s) 29, 333, 824, 874, 934, 1136, 1266, 1401, 1724
BslFI GGGAC 3 cut(s) 85, 369, 1616
BslI CCNNNNNNNGG 7 cut(s) 28, 1063, 1343, 1395, 1594, 1599, 2048
BsmAI GTCTC 5 cut(s) 271, 1094, 1262, 1851, 2585
BsmBI CGTCTC 1 cut(s) 1851
BsmFI GGGAC 3 cut(s) 85, 369, 1616
BsnI GGCC 6 cut(s) 397, 828, 1011, 1121, 1347, 1962
Bso31I GGTCTC 2 cut(s) 1094, 2585
Bsp119I TTCGAA 1 cut(s) 842
Bsp120I GGGCCC 1 cut(s) 826
Bsp1286I GDGCHC 5 cut(s) 349, 830, 912, 1956, 2041
Bsp1407I TGTACA 2 cut(s) 798, 1551
Bsp143I GATC 8 cut(s) 121, 592, 769, 1227, 1315, 1701, 2235, 2363
Bsp19I CCATGG 1 cut(s) 1570
BspACI CCGC 5 cut(s) 23, 55, 65, 937, 970
BspANI GGCC 6 cut(s) 397, 828, 1011, 1121, 1347, 1962
BspCNI CTCAG 8 cut(s) 193, 727, 1090, 1918, 1939, 1968, 2013, 2581
BspFNI CGCG 1 cut(s) 1232
BspHI TCATGA 2 cut(s) 366, 772
BspLI GGNNCC 7 cut(s) 73, 828, 897, 932, 1270, 1387, 1457
BspPI GGATC 1 cut(s) 2243
BspQI GCTCTTC 1 cut(s) 756
BspT104I TTCGAA 1 cut(s) 842
BspT107I GGYRCC 1 cut(s) 930
BspTI CTTAAG 2 cut(s) 350, 691
BspTNI GGTCTC 2 cut(s) 1094, 2585
BsrDI GCAATG 1 cut(s) 1080
BsrFI RCCGGY 3 cut(s) 873, 933, 1265
BsrGI TGTACA 2 cut(s) 798, 1551
BsrI ACTGG 6 cut(s) 1373, 1604, 1637, 1776, 2237, 2337
BssAI RCCGGY 3 cut(s) 873, 933, 1265
BssECI CCNNGG 7 cut(s) 781, 1292, 1389, 1401, 1451, 1570, 1593
BssMI GATC 8 cut(s) 121, 592, 769, 1227, 1315, 1701, 2235, 2363
BssSI CACGAG 1 cut(s) 342
BssT1I CCWWGG 4 cut(s) 781, 1451, 1570, 1593
Bst2BI CACGAG 1 cut(s) 342
Bst2UI CCWGG 4 cut(s) 610, 848, 1294, 1854
Bst4CI ACNGT 3 cut(s) 927, 1717, 1785
Bst6I CTCTTC 4 cut(s) 447, 555, 663, 756
BstAFI CTTAAG 2 cut(s) 350, 691
BstAUI TGTACA 2 cut(s) 798, 1551
BstBAI YACGTR 1 cut(s) 272
BstBI TTCGAA 1 cut(s) 842
BstC8I GCNNGC 3 cut(s) 65, 499, 935
BstDSI CCRYGG 1 cut(s) 1570
BstENI CCTNNNNNAGG 1 cut(s) 1592
BstF5I GGATG 7 cut(s) 480, 1554, 1626, 1828, 2010, 2422, 2497
BstFNI CGCG 1 cut(s) 1232
BstHHI GCGC 1 cut(s) 2534
BstKTI GATC 8 cut(s) 124, 595, 772, 1230, 1318, 1704, 2238, 2366
BstMAI GTCTC 5 cut(s) 271, 1094, 1262, 1851, 2585
BstMBI GATC 8 cut(s) 121, 592, 769, 1227, 1315, 1701, 2235, 2363
BstMWI GCNNNNNNNGC 2 cut(s) 99, 2366
BstNI CCWGG 4 cut(s) 610, 848, 1294, 1854
BstNSI RCATGY 4 cut(s) 734, 1284, 1656, 2486
BstSCI CCNGG 8 cut(s) 331, 608, 823, 846, 1292, 1400, 1723, 1852
BstSLI GKGCMC 3 cut(s) 349, 830, 2041
BstUI CGCG 1 cut(s) 1232
BstV1I GCAGC 5 cut(s) 12, 72, 90, 758, 2307
BstV2I GAAGAC 3 cut(s) 235, 1045, 2447
BstX2I RGATCY 2 cut(s) 1701, 2363
BstYI RGATCY 2 cut(s) 1701, 2363
BsuI GTATCC 1 cut(s) 1568
BsuRI GGCC 6 cut(s) 397, 828, 1011, 1121, 1347, 1962
BtgI CCRYGG 1 cut(s) 1570
BtsCI GGATG 7 cut(s) 480, 1554, 1626, 1828, 2010, 2422, 2497
BtsI GCAGTG 1 cut(s) 391
BtsIMutI CAGTG 5 cut(s) 391, 1701, 1783, 1963, 2344
Cac8I GCNNGC 3 cut(s) 65, 499, 935
CaiI CAGNNNCTG 1 cut(s) 1853
CciI TCATGA 2 cut(s) 366, 772
CfoI GCGC 1 cut(s) 2534
Cfr10I RCCGGY 3 cut(s) 873, 933, 1265
Cfr13I GGNCC 7 cut(s) 72, 826, 827, 1009, 1120, 1398, 2027
CseI GACGC 1 cut(s) 2452
Csp6I GTAC 5 cut(s) 799, 1525, 1552, 1585, 1786
CviQI GTAC 5 cut(s) 799, 1525, 1552, 1585, 1786
DpnI GATC 8 cut(s) 123, 594, 771, 1229, 1317, 1703, 2237, 2365
DpnII GATC 8 cut(s) 121, 592, 769, 1227, 1315, 1701, 2235, 2363
DraI TTTAAA 1 cut(s) 253
EaeI YGGCCR 2 cut(s) 395, 1345
Eam1104I CTCTTC 4 cut(s) 447, 555, 663, 756
EarI CTCTTC 4 cut(s) 447, 555, 663, 756
EciI GGCGGA 1 cut(s) 985
Ecl136II GAGCTC 1 cut(s) 1954
Eco130I CCWWGG 4 cut(s) 781, 1451, 1570, 1593
Eco24I GRGCYC 2 cut(s) 830, 1956
Eco31I GGTCTC 2 cut(s) 1094, 2585
Eco47I GGWCC 3 cut(s) 72, 1398, 2027
Eco53kI GAGCTC 1 cut(s) 1954
Eco57I CTGAAG 4 cut(s) 161, 684, 1830, 2369
Eco72I CACGTG 1 cut(s) 272
EcoICRI GAGCTC 1 cut(s) 1954
EcoNI CCTNNNNNAGG 1 cut(s) 1592
EcoRII CCWGG 4 cut(s) 608, 846, 1292, 1852
EcoT14I CCWWGG 4 cut(s) 781, 1451, 1570, 1593
EcoT22I ATGCAT 5 cut(s) 470, 517, 1007, 1519, 1654
EcoT38I GRGCYC 2 cut(s) 830, 1956
ErhI CCWWGG 4 cut(s) 781, 1451, 1570, 1593
Esp3I CGTCTC 1 cut(s) 1851
FalI AAGNNNNNCTT 4 cut(s) 220, 252, 2115, 2147
FaqI GGGAC 3 cut(s) 85, 369, 1616
FauI CCCGC 3 cut(s) 30, 48, 72
FauNDI CATATG 2 cut(s) 1746, 2032
FbaI TGATCA 2 cut(s) 769, 1315
FblI GTMKAC 2 cut(s) 315, 2201
Fnu4HI GCNGC 6 cut(s) 26, 61, 79, 747, 938, 2321
FokI GGATG 7 cut(s) 487, 1541, 1633, 1815, 2017, 2429, 2484
FriOI GRGCYC 2 cut(s) 830, 1956
Fsp4HI GCNGC 6 cut(s) 26, 61, 79, 747, 938, 2321
FspBI CTAG 7 cut(s) 68, 221, 725, 1427, 1949, 2159, 2361
GlaI GCGC 1 cut(s) 2533
GluI GCNGC 6 cut(s) 26, 61, 79, 747, 938, 2321
GsuI CTGGAG 1 cut(s) 1875
HaeIII GGCC 6 cut(s) 397, 828, 1011, 1121, 1347, 1962
HapII CCGG 9 cut(s) 29, 333, 824, 874, 934, 1136, 1266, 1401, 1724
HgaI GACGC 1 cut(s) 2452
HhaI GCGC 1 cut(s) 2534
Hin6I GCGC 1 cut(s) 2532
HinP1I GCGC 1 cut(s) 2532
HincII GTYRAC 2 cut(s) 207, 316
HindII GTYRAC 2 cut(s) 207, 316
HinfI GANTC 7 cut(s) 7, 203, 357, 482, 657, 1126, 1691
HpaII CCGG 9 cut(s) 29, 333, 824, 874, 934, 1136, 1266, 1401, 1724
HphI GGTGA 5 cut(s) 685, 1237, 1526, 1904, 2449
Hpy166II GTNNAC 7 cut(s) 207, 275, 316, 1552, 1781, 2039, 2202
Hpy188I TCNGA 8 cut(s) 180, 188, 528, 592, 664, 870, 900, 2373
Hpy8I GTNNAC 7 cut(s) 207, 275, 316, 1552, 1781, 2039, 2202
Hpy99I CGWCG 3 cut(s) 528, 1464, 2309
HpyAV CCTTC 7 cut(s) 304, 383, 550, 631, 1375, 1973, 2345
HpyCH4III ACNGT 3 cut(s) 927, 1717, 1785
HpyCH4IV ACGT 5 cut(s) 209, 271, 1731, 1886, 1939
HpyF10VI GCNNNNNNNGC 2 cut(s) 99, 2366
HpySE526I ACGT 5 cut(s) 209, 271, 1731, 1886, 1939
HspAI GCGC 1 cut(s) 2532
KroI GCCGGC 1 cut(s) 933
KroNI GCCGGC 1 cut(s) 935
Ksp22I TGATCA 2 cut(s) 769, 1315
Kzo9I GATC 8 cut(s) 121, 592, 769, 1227, 1315, 1701, 2235, 2363
LguI GCTCTTC 1 cut(s) 756
LmnI GCTCC 6 cut(s) 90, 191, 1094, 1167, 1855, 2096
Lsp1109I GCAGC 5 cut(s) 12, 72, 90, 758, 2307
LweI GCATC 5 cut(s) 502, 1837, 1995, 2347, 2506
MaeI CTAG 7 cut(s) 68, 221, 725, 1427, 1949, 2159, 2361
MaeII ACGT 5 cut(s) 209, 271, 1731, 1886, 1939
MaeIII GTNAC 2 cut(s) 550, 1711
MalI GATC 8 cut(s) 123, 594, 771, 1229, 1317, 1703, 2237, 2365
MboI GATC 8 cut(s) 121, 592, 769, 1227, 1315, 1701, 2235, 2363
MflI RGATCY 2 cut(s) 1701, 2363
MhlI GDGCHC 5 cut(s) 349, 830, 912, 1956, 2041
MlsI TGGCCA 1 cut(s) 397
MluNI TGGCCA 1 cut(s) 397
MlyI GAGTC 3 cut(s) 212, 351, 491
MmeI TCCRAC 4 cut(s) 506, 923, 2062, 2494
Mox20I TGGCCA 1 cut(s) 397
Mph1103I ATGCAT 5 cut(s) 470, 517, 1007, 1519, 1654
MroNI GCCGGC 1 cut(s) 933
MroXI GAANNNNTTC 1 cut(s) 2070
MscI TGGCCA 1 cut(s) 397
MslI CAYNNNNRTG 2 cut(s) 473, 1822
Msp20I TGGCCA 1 cut(s) 397
MspA1I CMGCKG 1 cut(s) 25
MspCI CTTAAG 2 cut(s) 350, 691
MspI CCGG 9 cut(s) 29, 333, 824, 874, 934, 1136, 1266, 1401, 1724
MspR9I CCNGG 8 cut(s) 333, 610, 825, 848, 1294, 1402, 1725, 1854
MvaI CCWGG 4 cut(s) 610, 848, 1294, 1854
MvnI CGCG 1 cut(s) 1232
MwoI GCNNNNNNNGC 2 cut(s) 99, 2366
NaeI GCCGGC 1 cut(s) 935
NciI CCSGG 4 cut(s) 333, 825, 1402, 1725
NcoI CCATGG 1 cut(s) 1570
NdeI CATATG 2 cut(s) 1746, 2032
NdeII GATC 8 cut(s) 121, 592, 769, 1227, 1315, 1701, 2235, 2363
NgoMIV GCCGGC 1 cut(s) 933
NlaIV GGNNCC 7 cut(s) 73, 828, 897, 932, 1270, 1387, 1457
NmuCI GTSAC 1 cut(s) 550
NsiI ATGCAT 5 cut(s) 470, 517, 1007, 1519, 1654
NspI RCATGY 4 cut(s) 734, 1284, 1656, 2486
NspV TTCGAA 1 cut(s) 842
PagI TCATGA 2 cut(s) 366, 772
PciSI GCTCTTC 1 cut(s) 756
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PdiI GCCGGC 1 cut(s) 935
PdmI GAANNNNTTC 1 cut(s) 2070
PfeI GAWTC 4 cut(s) 7, 657, 1126, 1691
PflMI CCANNNNNTGG 2 cut(s) 1063, 1599
PfoI TCCNGGA 2 cut(s) 1723, 1852
PkrI GCNGC 6 cut(s) 27, 62, 80, 748, 939, 2322
PleI GAGTC 3 cut(s) 211, 351, 490
PmaCI CACGTG 1 cut(s) 272
PmlI CACGTG 1 cut(s) 272
PpsI GAGTC 3 cut(s) 211, 351, 490
Ppu21I YACGTR 1 cut(s) 272
PshBI ATTAAT 1 cut(s) 2108
Psp124BI GAGCTC 1 cut(s) 1956
Psp6I CCWGG 4 cut(s) 608, 846, 1292, 1852
PspCI CACGTG 1 cut(s) 272
PspGI CCWGG 4 cut(s) 608, 846, 1292, 1852
PspN4I GGNNCC 7 cut(s) 73, 828, 897, 932, 1270, 1387, 1457
PspOMI GGGCCC 1 cut(s) 826
PspPI GGNCC 7 cut(s) 72, 826, 827, 1009, 1120, 1398, 2027
PstNI CAGNNNCTG 1 cut(s) 1853
PsuI RGATCY 2 cut(s) 1701, 2363
RsaI GTAC 5 cut(s) 800, 1526, 1553, 1586, 1787
RsaNI GTAC 5 cut(s) 799, 1525, 1552, 1585, 1786
RseI CAYNNNNRTG 2 cut(s) 473, 1822
SacI GAGCTC 1 cut(s) 1956
SalI GTCGAC 1 cut(s) 314
SapI GCTCTTC 1 cut(s) 756
SatI GCNGC 6 cut(s) 26, 61, 79, 747, 938, 2321
Sau3AI GATC 8 cut(s) 121, 592, 769, 1227, 1315, 1701, 2235, 2363
Sau96I GGNCC 7 cut(s) 72, 826, 827, 1009, 1120, 1398, 2027
SchI GAGTC 3 cut(s) 212, 351, 491
ScrFI CCNGG 8 cut(s) 333, 610, 825, 848, 1294, 1402, 1725, 1854
SduI GDGCHC 5 cut(s) 349, 830, 912, 1956, 2041
SfaNI GCATC 5 cut(s) 502, 1837, 1995, 2347, 2506
SfuI TTCGAA 1 cut(s) 842
SinI GGWCC 3 cut(s) 72, 1398, 2027
SmiMI CAYNNNNRTG 2 cut(s) 473, 1822
SmlI CTYRAG 6 cut(s) 257, 298, 350, 359, 691, 2046
SmoI CTYRAG 6 cut(s) 257, 298, 350, 359, 691, 2046
SsiI CCGC 5 cut(s) 23, 55, 65, 937, 970
SspMI CTAG 7 cut(s) 68, 221, 725, 1427, 1949, 2159, 2361
SstI GAGCTC 1 cut(s) 1956
StyD4I CCNGG 8 cut(s) 331, 608, 823, 846, 1292, 1400, 1723, 1852
StyI CCWWGG 4 cut(s) 781, 1451, 1570, 1593
TaaI ACNGT 3 cut(s) 927, 1717, 1785
TaiI ACGT 5 cut(s) 212, 274, 1734, 1889, 1942
TaqII GACCGA 1 cut(s) 2609
TatI WGTACW 3 cut(s) 798, 1551, 1785
TauI GCSGC 1 cut(s) 940
TfiI GAWTC 4 cut(s) 7, 657, 1126, 1691
TscAI CASTG 5 cut(s) 391, 1701, 1783, 1963, 2344
TseFI GTSAC 1 cut(s) 550
TseI GCWGC 5 cut(s) 25, 60, 78, 746, 2320
Tsp45I GTSAC 1 cut(s) 550
TspGWI ACGGA 1 cut(s) 1448
TspRI CASTG 5 cut(s) 391, 1701, 1783, 1963, 2344
Van91I CCANNNNNTGG 2 cut(s) 1063, 1599
Vha464I CTTAAG 2 cut(s) 350, 691
VneI GTGCAC 1 cut(s) 2037
VpaK11BI GGWCC 3 cut(s) 72, 1398, 2027
VspI ATTAAT 1 cut(s) 2108
XagI CCTNNNNNAGG 1 cut(s) 1592
XapI RAATTY 4 cut(s) 572, 585, 720, 1025
XbaI TCTAGA 2 cut(s) 724, 1948
XceI RCATGY 4 cut(s) 734, 1284, 1656, 2486
XcmI CCANNNNNNNNNTGG 2 cut(s) 2405, 2410
XmiI GTMKAC 2 cut(s) 315, 2201
XmnI GAANNNNTTC 1 cut(s) 2070
XspI CTAG 7 cut(s) 68, 221, 725, 1427, 1949, 2159, 2361
Zsp2I ATGCAT 5 cut(s) 470, 517, 1007, 1519, 1654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.