Rroxscaffold_6G00423640

Respiratory burst

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005589
Physical Location & Seq
Reverse (-)
44696710 .. 44701426
4717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00423640.1

Sequence Viewer

Length: 2526 bp
ATGTTCAAAGAAGACGATCAATGCTATGTGGAAGTGGCACTCCATGTTTGCGACGACAATACCTTTGGGCTTCGGAACATCAGAGGAGCTGAACCTGAGTCGACGTCAGCAAGGCTAGAGAGAAGACGATCCGTTGCTTCTCAACTCTCTTTTAAACTCAAGAAAGTCTCACGTGAACTGAAGCGCATGAAATCTTCCTTGAGCTTCAAGAAGGTCGACAGCCCCACCAAGCCCGGTATTGCTCGTGCCCTTAAGGGACTCAAGCTCATGACCAAAAATGTGTGCAGTGAAGGTTGGCCAGAGATCGAAATAAGGTTTGATGAATTGGCTGTCGATGGAACTCTTCCAAAATCAGTCTTTGCTCAATGCATAGGGATGAAAGAGTCGAGTGAGTTTGCTTGCGAAGTATTCGATGCATTGGCTCGTCGGAGCGGAATAACATCTTGTTGTGTGACCAAGGAAGAGTTGAGAGAATTTTGGGAACAAATTTCAGATCACAGCTTCAATGCCAGGCTTCAAACCTTCTTTGACATGGTGGATAAAAATGCAGATGGTCGAATCTCTGAAGAAGAGGTGAAAGAGATTATCACCTTAAGTGCCTCTGCTAATAAGCTGTCAAAAATTCTAGAACATGCTGAGGAATATGCAGCATTGATTATGGAAGAGCTTGATCATGACAACCTTGGATATATTGATTTGTACAACTTGAAAATGCTACTTCTTCCGGGCCCAACCCAATCTTCGAACCTGGAAACAGACAGCTACATTCAGAGCCGGTTGCTAAGTGAAAAGCTGGTTCCGACCAAAGAGCACAACCCTATAAGGCGGTGGTGCCGGCGGCTGTCCTACTTTGTTGAGGATAATTGGAGGCGGATTTGGGTCATTCTGCTATGGCTTTTTATATGCATGGGCCTCTTTGCTTGGAAATTCATTCAGTATAGAGATAGGGAGGTCTTCCATGTAATGGGCTATTGTGTGTGCATTGCCAAAGGTGGAGCTGAGACCCTAAAGTTTAACATGGCCCTGATTCTTCTTCCGGTGTGTAGAAATACAATTACTTGGCTAAGAAGCAAGACCAAGTTAGGAGGTATAGTTCCATTTGATGACAATATCAATTTTCATAAGGTGATCGCGTTTGGAATTGTAGTTGGGGTCAGTCTCCATGCCGGTTCCCATCTTACATGCGACTTCCCTAGGCTACTACACTCAACCAATGATCAATACGACAAGATGAAGCCATTTTTCGGCCAAAAACGACCCGACAACTACTGGTGGTTTCTGAAGGGAACCGAGGGCTGGACCGGGGTGGTTATGGTGGCACTAATGCTAGTTGCATACACATTGGCTCAACCTTGGTTCCGTCGCAATCGACTAAGAAACCTCCCTAAAACCTTAAAGAAAATTACTGGGTTCAATGCATTTTGGTACTCCCATCACCTCTTTATCTTTGTGTACATCCTCCTTATTATCCATGGATACTTCCTCTACCTCTCTAAGGACTGGGACAAGAAAACAACATGGATGTATCTGGCTTTCCCAGTAATAATATATGCATGTGAGCGTTTGCTTCGTGCTTTCAGGTCTGGATATGAATCAGTGAAGATCTTGAAGGTTACTGTTTACCCGGGAAACGTATTGGCACTTCATATGTCTAAACCACAGGGATTAAAATATACCAGTGGACAGTACATTTACATAAATTGTCCTGCTATTTCCTCATTTCAATGGCATCCATTCTCAATTACTTCAGCTCCTGGAGACGACTATCTAAGTGTTCATATTCGCACGTTGGGTGATTGGACATCTCAGCTCAAATACATTTTCTCTCAGGTTTGCCAAAGTCCTCCTCTAGAGCTCAGTGGCCTTCTAAGAGCTGATACTGAACAACCCGATATACAACTCAGGATGCCAAGACTTCTTATTGATGGTCCATATGGTGCACCAGCTCAAGACTACAAAAACTACGAAGTTCTTCTACTTGTTGGACTTGGAATTGGAGCAACTCCATTAATAAGCATACTCAAAGATGTGCTTAACAACATTAAGCGACAGAAAGAACTAGAAGCTCAAATGTTGGTGGAAAGTGGTGTTAAGAGAAAGTCTACTTTTGCAACAAAACAAGCTTATTTCTACTGGATCACAAGAGAGCAAGGCTCGTTCGAGTGGTTTAGGGGTGTGATGAATGAGGTCGCAGAAAATGACCTCGACGGCGTGATTGAGCTGCACAACTACTGCACCAGTGTCTATGAAGAAGGAGATGCTAGATCAGCTCTGATTACTATGCTTCAGTCTATAAACCATGCCAAAAATGGTGTGGATGTTGTATCAGGAACGAGGGTGAAGACGCATTTTGCAAGACCAAAATGGCGTAATGTTTTTAAGCATGTGGCAGTAAAGCATCCTGATCAAAAAGTTGGAGTGTTTTATTGTGGCGCACATGGATTAGTTGGGGAGTTAAAGAGGTTAGCTAAAGACTTTTCCAGAAAAACTGAGACCAAGTTTGATTTCCACAAAGAGAACTTTTAG

Protein Analysis

841

Amino Acids

96.85

Weight (kDa)

8.98

Isoelectric Point (pI)

43.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADPH_Ox PF08414 78 - 170 1.4e-31 Respiratory burst NADPH oxidase
EF-hand_7 PF13499 171 - 233 5.9e-06 EF-hand domain pair
Ferric_reduct PF01794 339 - 489 9.2e-12 Ferric reductase like transmembrane component
FAD_binding_8 PF08022 532 - 649 1.9e-35 FAD-binding domain
NAD_binding_6 PF08030 655 - 823 2.5e-50 Ferric reductase NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 107
AccB1I GGYRCC 1 cut(s) 831
AccB7I CCANNNNNTGG 1 cut(s) 964
AccBSI CCGCTC 1 cut(s) 432
AccI GTMKAC 3 cut(s) 101, 216, 2102
AccII CGCG 1 cut(s) 1133
AciI CCGC 4 cut(s) 432, 826, 838, 871
AclWI GGATC 2 cut(s) 123, 2144
AcoI YGGCCR 2 cut(s) 296, 1246
AcsI RAATTY 4 cut(s) 473, 486, 621, 926
AcuI CTGAAG 5 cut(s) 200, 585, 1301, 1731, 2270
AcvI CACGTG 1 cut(s) 173
AcyI GRCGYC 1 cut(s) 104
AfaI GTAC 4 cut(s) 701, 1427, 1454, 1688
AfiI CCNNNNNNNGG 4 cut(s) 964, 1244, 1296, 1495
AflII CTTAAG 2 cut(s) 251, 592
AgsI TTSAA 8 cut(s) 7, 208, 505, 518, 709, 1414, 1609, 1724
AjnI CCWGG 3 cut(s) 509, 747, 1753
AjuI GAANNNNNNNTTGG 4 cut(s) 724, 756, 2489, 2521
AloI GAACNNNNNNTCC 2 cut(s) 1077, 1109
Alw21I GWGCWC 3 cut(s) 813, 1857, 1942
Alw26I GTCTC 5 cut(s) 172, 995, 1163, 1752, 2486
Alw44I GTGCAC 1 cut(s) 1938
AlwI GGATC 2 cut(s) 123, 2144
AlwNI CAGNNNCTG 1 cut(s) 1754
Ama87I CYCGRG 1 cut(s) 1624
AoxI GGCC 6 cut(s) 296, 727, 910, 1020, 1246, 1861
ApaI GGGCCC 1 cut(s) 731
ApaLI GTGCAC 1 cut(s) 1938
ApeKI GCWGC 2 cut(s) 647, 2221
ApoI RAATTY 4 cut(s) 473, 486, 621, 926
ArsI GACNNNNNNTTYG 2 cut(s) 47, 79
AseI ATTAAT 1 cut(s) 2009
Asp700I GAANNNNTTC 1 cut(s) 1971
AspA2I CCTAGG 1 cut(s) 1193
AspLEI GCGC 2 cut(s) 186, 2435
AspS9I GGNCC 6 cut(s) 727, 728, 910, 1021, 1299, 1928
AsuC2I CCSGG 5 cut(s) 234, 726, 1303, 1625, 1626
AsuHPI GGTGA 6 cut(s) 580, 586, 1138, 1427, 1805, 2350
AsuII TTCGAA 1 cut(s) 743
AvaI CYCGRG 1 cut(s) 1624
AvaII GGWCC 2 cut(s) 1299, 1928
AvrII CCTAGG 1 cut(s) 1193
BaeGI GKGCMC 3 cut(s) 250, 731, 1942
BalI TGGCCA 1 cut(s) 298
BanI GGYRCC 1 cut(s) 831
BanII GRGCYC 2 cut(s) 731, 1857
BarI GAAGNNNNNNTAC 4 cut(s) 1626, 1658, 1959, 1991
BauI CACGAG 1 cut(s) 243
BbrPI CACGTG 1 cut(s) 173
BbsI GAAGAC 4 cut(s) 18, 130, 946, 2348
Bbv12I GWGCWC 3 cut(s) 813, 1857, 1942
BbvCI CCTCAGC 1 cut(s) 636
BbvI GCAGC 2 cut(s) 659, 2208
BccI CCATC 5 cut(s) 329, 545, 1182, 1440, 1919
BceAI ACGGC 1 cut(s) 2224
BciT130I CCWGG 3 cut(s) 511, 749, 1755
BciVI GTATCC 1 cut(s) 1469
BclI TGATCA 3 cut(s) 670, 1216, 2404
BcnI CCSGG 5 cut(s) 234, 726, 1303, 1625, 1626
BcoDI GTCTC 5 cut(s) 172, 995, 1163, 1752, 2486
BfaI CTAG 7 cut(s) 116, 626, 1194, 1328, 1850, 2060, 2262
BfrI CTTAAG 2 cut(s) 251, 592
BfuI GTATCC 1 cut(s) 1469
BglII AGATCT 1 cut(s) 1602
BisI GCNGC 3 cut(s) 648, 839, 2222
BlnI CCTAGG 1 cut(s) 1193
BlsI GCNGC 3 cut(s) 649, 840, 2223
Bme1390I CCNGG 8 cut(s) 234, 511, 726, 749, 1303, 1625, 1626, 1755
Bme18I GGWCC 2 cut(s) 1299, 1928
BmeT110I CYCGRG 1 cut(s) 1624
BmgT120I GGNCC 6 cut(s) 727, 728, 910, 1021, 1299, 1928
BmiI GGNNCC 6 cut(s) 729, 798, 833, 1171, 1288, 1358
BmrFI CCNGG 8 cut(s) 234, 511, 726, 749, 1303, 1625, 1626, 1755
BmrI ACTGGG 3 cut(s) 1416, 1510, 1532
BmsI GCATC 5 cut(s) 403, 1738, 1896, 2248, 2407
BmuI ACTGGG 3 cut(s) 1416, 1510, 1532
BpiI GAAGAC 4 cut(s) 18, 130, 946, 2348
BplI GAGNNNNNCTC 2 cut(s) 2138, 2170
BpmI CTGGAG 1 cut(s) 1776
Bpu10I CCTNAGC 1 cut(s) 636
Bpu14I TTCGAA 1 cut(s) 743
BpuEI CTTGAG 4 cut(s) 143, 220, 245, 1932
BpuMI CCSGG 5 cut(s) 234, 726, 1303, 1625, 1626
BsaAI YACGTR 1 cut(s) 173
BsaBI GATNNNNATC 1 cut(s) 1591
BsaHI GRCGYC 1 cut(s) 104
BsaI GGTCTC 2 cut(s) 995, 2486
BsaJI CCNNGG 8 cut(s) 456, 682, 1193, 1290, 1302, 1352, 1471, 1624
BsaWI WCCGGW 1 cut(s) 1036
BsaXI ACNNNNNCTCC 4 cut(s) 1077, 1107, 1735, 1765
Bsc4I CCNNNNNNNGG 4 cut(s) 964, 1244, 1296, 1495
Bse118I RCCGGY 3 cut(s) 774, 834, 1166
Bse1I ACTGG 7 cut(s) 1274, 1411, 1505, 1538, 1677, 2138, 2238
Bse3DI GCAATG 1 cut(s) 981
Bse8I GATNNNNATC 1 cut(s) 1591
BseBI CCWGG 3 cut(s) 511, 749, 1755
BseDI CCNNGG 8 cut(s) 456, 682, 1193, 1290, 1302, 1352, 1471, 1624
BseGI GGATG 7 cut(s) 381, 1455, 1527, 1729, 1911, 2323, 2398
BseJI GATNNNNATC 1 cut(s) 1591
BseLI CCNNNNNNNGG 4 cut(s) 964, 1244, 1296, 1495
BseMI GCAATG 1 cut(s) 981
BseMII CTCAG 8 cut(s) 87, 627, 990, 1820, 1841, 1870, 1915, 2481
BseNI ACTGG 7 cut(s) 1274, 1411, 1505, 1538, 1677, 2138, 2238
BseRI GAGGAG 2 cut(s) 99, 1836
BseSI GKGCMC 3 cut(s) 250, 731, 1942
BseXI GCAGC 2 cut(s) 659, 2208
BsgI GTGCAG 3 cut(s) 304, 2207, 2218
Bsh1236I CGCG 1 cut(s) 1133
BshFI GGCC 6 cut(s) 298, 729, 912, 1022, 1248, 1863
BshNI GGYRCC 1 cut(s) 831
BsiHKAI GWGCWC 3 cut(s) 813, 1857, 1942
BsiHKCI CYCGRG 1 cut(s) 1624
BsiSI CCGG 8 cut(s) 234, 725, 775, 835, 1037, 1167, 1302, 1625
BslFI GGGAC 2 cut(s) 270, 1517
BslI CCNNNNNNNGG 4 cut(s) 964, 1244, 1296, 1495
BsmAI GTCTC 5 cut(s) 172, 995, 1163, 1752, 2486
BsmBI CGTCTC 1 cut(s) 1752
BsmFI GGGAC 2 cut(s) 270, 1517
BsnI GGCC 6 cut(s) 298, 729, 912, 1022, 1248, 1863
Bso31I GGTCTC 2 cut(s) 995, 2486
BsoBI CYCGRG 1 cut(s) 1624
Bsp119I TTCGAA 1 cut(s) 743
Bsp120I GGGCCC 1 cut(s) 727
Bsp1286I GDGCHC 5 cut(s) 250, 731, 813, 1857, 1942
Bsp1407I TGTACA 2 cut(s) 699, 1452
Bsp19I CCATGG 1 cut(s) 1471
BspACI CCGC 4 cut(s) 432, 826, 838, 871
BspANI GGCC 6 cut(s) 298, 729, 912, 1022, 1248, 1863
BspCNI CTCAG 8 cut(s) 88, 628, 991, 1819, 1840, 1869, 1914, 2482
BspFNI CGCG 1 cut(s) 1133
BspHI TCATGA 2 cut(s) 267, 673
BspLI GGNNCC 6 cut(s) 729, 798, 833, 1171, 1288, 1358
BspPI GGATC 2 cut(s) 123, 2144
BspQI GCTCTTC 1 cut(s) 657
BspT104I TTCGAA 1 cut(s) 743
BspT107I GGYRCC 1 cut(s) 831
BspTI CTTAAG 2 cut(s) 251, 592
BspTNI GGTCTC 2 cut(s) 995, 2486
BsrBI CCGCTC 1 cut(s) 432
BsrDI GCAATG 1 cut(s) 981
BsrFI RCCGGY 3 cut(s) 774, 834, 1166
BsrGI TGTACA 2 cut(s) 699, 1452
BsrI ACTGG 7 cut(s) 1274, 1411, 1505, 1538, 1677, 2138, 2238
BssAI RCCGGY 3 cut(s) 774, 834, 1166
BssECI CCNNGG 8 cut(s) 456, 682, 1193, 1290, 1302, 1352, 1471, 1624
BssNI GRCGYC 1 cut(s) 104
BssSI CACGAG 1 cut(s) 243
BssT1I CCWWGG 5 cut(s) 456, 682, 1193, 1352, 1471
Bst2BI CACGAG 1 cut(s) 243
Bst2UI CCWGG 3 cut(s) 511, 749, 1755
Bst4CI ACNGT 2 cut(s) 1618, 1686
Bst6I CTCTTC 4 cut(s) 348, 456, 564, 657
BstACI GRCGYC 1 cut(s) 104
BstAFI CTTAAG 2 cut(s) 251, 592
BstAUI TGTACA 2 cut(s) 699, 1452
BstBAI YACGTR 1 cut(s) 173
BstBI TTCGAA 1 cut(s) 743
BstC8I GCNNGC 2 cut(s) 400, 836
BstDSI CCRYGG 1 cut(s) 1471
BstENI CCTNNNNNAGG 1 cut(s) 1493
BstF5I GGATG 7 cut(s) 381, 1455, 1527, 1729, 1911, 2323, 2398
BstFNI CGCG 1 cut(s) 1133
BstHHI GCGC 2 cut(s) 186, 2435
BstMAI GTCTC 5 cut(s) 172, 995, 1163, 1752, 2486
BstMWI GCNNNNNNNGC 1 cut(s) 2267
BstNI CCWGG 3 cut(s) 511, 749, 1755
BstNSI RCATGY 4 cut(s) 635, 1185, 1557, 2387
BstSCI CCNGG 8 cut(s) 232, 509, 724, 747, 1301, 1623, 1624, 1753
BstSLI GKGCMC 3 cut(s) 250, 731, 1942
BstUI CGCG 1 cut(s) 1133
BstV1I GCAGC 2 cut(s) 659, 2208
BstV2I GAAGAC 4 cut(s) 18, 130, 946, 2348
BstX2I RGATCY 1 cut(s) 1602
BstYI RGATCY 1 cut(s) 1602
BsuI GTATCC 1 cut(s) 1469
BsuRI GGCC 6 cut(s) 298, 729, 912, 1022, 1248, 1863
BtgI CCRYGG 1 cut(s) 1471
BtsCI GGATG 7 cut(s) 381, 1455, 1527, 1729, 1911, 2323, 2398
BtsI GCAGTG 1 cut(s) 292
BtsIMutI CAGTG 5 cut(s) 292, 1602, 1684, 1864, 2245
Cac8I GCNNGC 2 cut(s) 400, 836
CaiI CAGNNNCTG 1 cut(s) 1754
CciI TCATGA 2 cut(s) 267, 673
CfoI GCGC 2 cut(s) 186, 2435
Cfr10I RCCGGY 3 cut(s) 774, 834, 1166
Cfr13I GGNCC 6 cut(s) 727, 728, 910, 1021, 1299, 1928
Cfr9I CCCGGG 1 cut(s) 1624
CseI GACGC 1 cut(s) 2353
Csp6I GTAC 4 cut(s) 700, 1426, 1453, 1687
CviQI GTAC 4 cut(s) 700, 1426, 1453, 1687
DraI TTTAAA 1 cut(s) 154
EaeI YGGCCR 2 cut(s) 296, 1246
Eam1104I CTCTTC 4 cut(s) 348, 456, 564, 657
EarI CTCTTC 4 cut(s) 348, 456, 564, 657
EciI GGCGGA 1 cut(s) 886
Ecl136II GAGCTC 1 cut(s) 1855
Eco130I CCWWGG 5 cut(s) 456, 682, 1193, 1352, 1471
Eco24I GRGCYC 2 cut(s) 731, 1857
Eco31I GGTCTC 2 cut(s) 995, 2486
Eco47I GGWCC 2 cut(s) 1299, 1928
Eco53kI GAGCTC 1 cut(s) 1855
Eco57I CTGAAG 5 cut(s) 200, 585, 1301, 1731, 2270
Eco72I CACGTG 1 cut(s) 173
Eco88I CYCGRG 1 cut(s) 1624
EcoICRI GAGCTC 1 cut(s) 1855
EcoNI CCTNNNNNAGG 1 cut(s) 1493
EcoRII CCWGG 3 cut(s) 509, 747, 1753
EcoT14I CCWWGG 5 cut(s) 456, 682, 1193, 1352, 1471
EcoT22I ATGCAT 5 cut(s) 371, 418, 908, 1420, 1555
EcoT38I GRGCYC 2 cut(s) 731, 1857
ErhI CCWWGG 5 cut(s) 456, 682, 1193, 1352, 1471
Esp3I CGTCTC 1 cut(s) 1752
FalI AAGNNNNNCTT 2 cut(s) 2016, 2048
FaqI GGGAC 2 cut(s) 270, 1517
FauNDI CATATG 2 cut(s) 1647, 1933
FbaI TGATCA 3 cut(s) 670, 1216, 2404
FblI GTMKAC 3 cut(s) 101, 216, 2102
Fnu4HI GCNGC 3 cut(s) 648, 839, 2222
FokI GGATG 7 cut(s) 388, 1442, 1534, 1716, 1918, 2330, 2385
FriOI GRGCYC 2 cut(s) 731, 1857
Fsp4HI GCNGC 3 cut(s) 648, 839, 2222
FspBI CTAG 7 cut(s) 116, 626, 1194, 1328, 1850, 2060, 2262
GlaI GCGC 2 cut(s) 185, 2434
GluI GCNGC 3 cut(s) 648, 839, 2222
GsuI CTGGAG 1 cut(s) 1776
HaeIII GGCC 6 cut(s) 298, 729, 912, 1022, 1248, 1863
HapII CCGG 8 cut(s) 234, 725, 775, 835, 1037, 1167, 1302, 1625
HgaI GACGC 1 cut(s) 2353
HhaI GCGC 2 cut(s) 186, 2435
Hin1I GRCGYC 1 cut(s) 104
Hin6I GCGC 2 cut(s) 184, 2433
HinP1I GCGC 2 cut(s) 184, 2433
HincII GTYRAC 2 cut(s) 102, 217
HindII GTYRAC 2 cut(s) 102, 217
HindIII AAGCTT 1 cut(s) 2121
HinfI GANTC 6 cut(s) 98, 258, 383, 558, 1027, 1592
HpaII CCGG 8 cut(s) 234, 725, 775, 835, 1037, 1167, 1302, 1625
HphI GGTGA 6 cut(s) 580, 586, 1138, 1427, 1805, 2350
Hpy166II GTNNAC 8 cut(s) 102, 176, 217, 1453, 1621, 1682, 1940, 2103
Hpy188I TCNGA 9 cut(s) 75, 83, 429, 493, 565, 771, 801, 1281, 2274
Hpy8I GTNNAC 8 cut(s) 102, 176, 217, 1453, 1621, 1682, 1940, 2103
Hpy99I CGWCG 5 cut(s) 56, 106, 429, 1365, 2210
HpyAV CCTTC 7 cut(s) 205, 284, 532, 1276, 1603, 1874, 2246
HpyCH4III ACNGT 2 cut(s) 1618, 1686
HpyCH4IV ACGT 4 cut(s) 104, 172, 1632, 1787
HpyF10VI GCNNNNNNNGC 1 cut(s) 2267
HpySE526I ACGT 4 cut(s) 104, 172, 1632, 1787
Hsp92I GRCGYC 1 cut(s) 104
HspAI GCGC 2 cut(s) 184, 2433
KroI GCCGGC 1 cut(s) 834
KroNI GCCGGC 1 cut(s) 836
Ksp22I TGATCA 3 cut(s) 670, 1216, 2404
LguI GCTCTTC 1 cut(s) 657
LmnI GCTCC 5 cut(s) 86, 429, 995, 1756, 1997
Lsp1109I GCAGC 2 cut(s) 659, 2208
LweI GCATC 5 cut(s) 403, 1738, 1896, 2248, 2407
MaeI CTAG 7 cut(s) 116, 626, 1194, 1328, 1850, 2060, 2262
MaeII ACGT 4 cut(s) 104, 172, 1632, 1787
MaeIII GTNAC 2 cut(s) 451, 1612
MbiI CCGCTC 1 cut(s) 432
MflI RGATCY 1 cut(s) 1602
MhlI GDGCHC 5 cut(s) 250, 731, 813, 1857, 1942
MlsI TGGCCA 1 cut(s) 298
MluNI TGGCCA 1 cut(s) 298
MlyI GAGTC 3 cut(s) 107, 252, 392
MmeI TCCRAC 4 cut(s) 407, 824, 1963, 2395
Mox20I TGGCCA 1 cut(s) 298
Mph1103I ATGCAT 5 cut(s) 371, 418, 908, 1420, 1555
MroNI GCCGGC 1 cut(s) 834
MroXI GAANNNNTTC 1 cut(s) 1971
MscI TGGCCA 1 cut(s) 298
MslI CAYNNNNRTG 2 cut(s) 374, 1723
Msp20I TGGCCA 1 cut(s) 298
MspCI CTTAAG 2 cut(s) 251, 592
MspI CCGG 8 cut(s) 234, 725, 775, 835, 1037, 1167, 1302, 1625
MspR9I CCNGG 8 cut(s) 234, 511, 726, 749, 1303, 1625, 1626, 1755
MvaI CCWGG 3 cut(s) 511, 749, 1755
MvnI CGCG 1 cut(s) 1133
MwoI GCNNNNNNNGC 1 cut(s) 2267
NaeI GCCGGC 1 cut(s) 836
NciI CCSGG 5 cut(s) 234, 726, 1303, 1625, 1626
NcoI CCATGG 1 cut(s) 1471
NdeI CATATG 2 cut(s) 1647, 1933
NgoMIV GCCGGC 1 cut(s) 834
NlaIV GGNNCC 6 cut(s) 729, 798, 833, 1171, 1288, 1358
NmuCI GTSAC 1 cut(s) 451
NsiI ATGCAT 5 cut(s) 371, 418, 908, 1420, 1555
NspI RCATGY 4 cut(s) 635, 1185, 1557, 2387
NspV TTCGAA 1 cut(s) 743
PagI TCATGA 2 cut(s) 267, 673
PciSI GCTCTTC 1 cut(s) 657
PdiI GCCGGC 1 cut(s) 836
PdmI GAANNNNTTC 1 cut(s) 1971
PfeI GAWTC 3 cut(s) 558, 1027, 1592
PflMI CCANNNNNTGG 1 cut(s) 964
PfoI TCCNGGA 1 cut(s) 1753
PkrI GCNGC 3 cut(s) 649, 840, 2223
PleI GAGTC 3 cut(s) 106, 252, 391
PmaCI CACGTG 1 cut(s) 173
PmlI CACGTG 1 cut(s) 173
PpsI GAGTC 3 cut(s) 106, 252, 391
Ppu21I YACGTR 1 cut(s) 173
PshBI ATTAAT 1 cut(s) 2009
Psp124BI GAGCTC 1 cut(s) 1857
Psp6I CCWGG 3 cut(s) 509, 747, 1753
PspCI CACGTG 1 cut(s) 173
PspGI CCWGG 3 cut(s) 509, 747, 1753
PspN4I GGNNCC 6 cut(s) 729, 798, 833, 1171, 1288, 1358
PspOMI GGGCCC 1 cut(s) 727
PspPI GGNCC 6 cut(s) 727, 728, 910, 1021, 1299, 1928
PstNI CAGNNNCTG 1 cut(s) 1754
PsuI RGATCY 1 cut(s) 1602
RsaI GTAC 4 cut(s) 701, 1427, 1454, 1688
RsaNI GTAC 4 cut(s) 700, 1426, 1453, 1687
RseI CAYNNNNRTG 2 cut(s) 374, 1723
SacI GAGCTC 1 cut(s) 1857
SalI GTCGAC 2 cut(s) 100, 215
SapI GCTCTTC 1 cut(s) 657
SatI GCNGC 3 cut(s) 648, 839, 2222
Sau96I GGNCC 6 cut(s) 727, 728, 910, 1021, 1299, 1928
SchI GAGTC 3 cut(s) 107, 252, 392
ScrFI CCNGG 8 cut(s) 234, 511, 726, 749, 1303, 1625, 1626, 1755
SduI GDGCHC 5 cut(s) 250, 731, 813, 1857, 1942
SfaNI GCATC 5 cut(s) 403, 1738, 1896, 2248, 2407
SfuI TTCGAA 1 cut(s) 743
SinI GGWCC 2 cut(s) 1299, 1928
SmaI CCCGGG 1 cut(s) 1626
SmiMI CAYNNNNRTG 2 cut(s) 374, 1723
SmlI CTYRAG 6 cut(s) 158, 199, 251, 260, 592, 1947
SmoI CTYRAG 6 cut(s) 158, 199, 251, 260, 592, 1947
SsiI CCGC 4 cut(s) 432, 826, 838, 871
SspMI CTAG 7 cut(s) 116, 626, 1194, 1328, 1850, 2060, 2262
SstI GAGCTC 1 cut(s) 1857
StyD4I CCNGG 8 cut(s) 232, 509, 724, 747, 1301, 1623, 1624, 1753
StyI CCWWGG 5 cut(s) 456, 682, 1193, 1352, 1471
TaaI ACNGT 2 cut(s) 1618, 1686
TaiI ACGT 4 cut(s) 107, 175, 1635, 1790
TatI WGTACW 3 cut(s) 699, 1452, 1686
TauI GCSGC 1 cut(s) 841
TfiI GAWTC 3 cut(s) 558, 1027, 1592
TscAI CASTG 5 cut(s) 292, 1602, 1684, 1864, 2245
TseFI GTSAC 1 cut(s) 451
TseI GCWGC 2 cut(s) 647, 2221
Tsp45I GTSAC 1 cut(s) 451
TspGWI ACGGA 2 cut(s) 121, 1349
TspMI CCCGGG 1 cut(s) 1624
TspRI CASTG 5 cut(s) 292, 1602, 1684, 1864, 2245
Van91I CCANNNNNTGG 1 cut(s) 964
Vha464I CTTAAG 2 cut(s) 251, 592
VneI GTGCAC 1 cut(s) 1938
VpaK11BI GGWCC 2 cut(s) 1299, 1928
VspI ATTAAT 1 cut(s) 2009
XagI CCTNNNNNAGG 1 cut(s) 1493
XapI RAATTY 4 cut(s) 473, 486, 621, 926
XbaI TCTAGA 2 cut(s) 625, 1849
XceI RCATGY 4 cut(s) 635, 1185, 1557, 2387
XcmI CCANNNNNNNNNTGG 2 cut(s) 2306, 2311
XmaI CCCGGG 1 cut(s) 1624
XmaJI CCTAGG 1 cut(s) 1193
XmiI GTMKAC 3 cut(s) 101, 216, 2102
XmnI GAANNNNTTC 1 cut(s) 1971
XspI CTAG 7 cut(s) 116, 626, 1194, 1328, 1850, 2060, 2262
ZraI GACGTC 1 cut(s) 105
Zsp2I ATGCAT 5 cut(s) 371, 418, 908, 1420, 1555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.