RLG00000029666
ERF Family

Performs the first committed step in the biosynthesis of isoprene-containing compounds such as sterols and terpenoids

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0010613
Physical Location & Seq
Reverse (-)
42526806 .. 42529601
2796 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029666

Sequence Viewer

Length: 552 bp
ATGGATAAAGAATTCCAAGGGGACCGGATTCGTGTTCTTTATACCTCTCAAACTGGGTTTGTTTTAGTAGCAAATATGTTTCCATACTCGGTTTTTGCCCTCGGAAAGTTAATGAATGTAAAGGAAGATCAGAGCCAGCTTTCTGCTATAGCAAGGCAAGGTTCAGGCAGTGCTTGTCGCAGCTTACATGGGGGATTTGTCAAGTGGATCATGGGAAAAGATGACAAGGGAACCGACAGTCTTGCAGTTCAACTTGCAGACGAGAAGCACTGGGATGAGCTTGTCATTATTATTGCTGTGAACGTTGATTACAAAAGCTCCGAGTCCGACCAACCCAACCACGACGATCACTTCAAGTCCGATTCCGGCCTTCTCCCCTGCACTCATCCCGCTGTGATTACAGAAGCTCCGAGTCCGTTCAACTTGACTATATCTGCAGCAGAACCCGGCAGCGGTGATGGAGAATCGGACGTCGGAGGTGCCAGAGGTAAAGCGGAACTCAGTGAGTTTGTTGGCGCCCTAGCCACTCCTTTGGATCCTCTCACCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004163 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0006066 GO:0006084 GO:0006139 GO:0006163 GO:0006464 GO:0006486 GO:0006487 GO:0006488 GO:0006489 GO:0006490 GO:0006629 GO:0006637 GO:0006644 GO:0006694 GO:0006695 GO:0006720 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008202 GO:0008203 GO:0008284 GO:0008299 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009117 GO:0009150 GO:0009240 GO:0009259 GO:0009889 GO:0009987 GO:0016125 GO:0016126 GO:0016829 GO:0016830 GO:0016831 GO:0019216 GO:0019218 GO:0019222 GO:0019287 GO:0019538 GO:0019637 GO:0019693 GO:0030544 GO:0031072 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0034645 GO:0035383 GO:0036211 GO:0042127 GO:0042579 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043603 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0045540 GO:0046165 GO:0046465 GO:0046483 GO:0046490 GO:0046890 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050810 GO:0051186 GO:0055086 GO:0062012 GO:0065007 GO:0070085 GO:0071704 GO:0072521 GO:0080090 GO:0090181 GO:0090407 GO:0106118 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901615 GO:1901617 GO:1902652 GO:1902653 GO:1902930
KEGG Pathways
Metabolic & Signaling
ko00900 ko01100 ko01110 ko01130 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
GHMP_kinases_N

Protein Analysis

184

Amino Acids

19.64

Weight (kDa)

4.82

Isoelectric Point (pI)

31.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MVD-like_N PF22700 31 - 82 8.9e-16 Diphosphomevalonate decarboxylase-like N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 474
AccB1I GGYRCC 2 cut(s) 479, 515
AciI CCGC 3 cut(s) 390, 453, 494
AclI AACGTT 1 cut(s) 303
AclWI GGATC 3 cut(s) 215, 530, 543
AcsI RAATTY 1 cut(s) 11
AcyI GRCGYC 2 cut(s) 471, 516
AfiI CCNNNNNNNGG 1 cut(s) 452
AgsI TTSAA 3 cut(s) 251, 355, 421
AluBI AGCT 5 cut(s) 139, 183, 280, 318, 407
AluI AGCT 5 cut(s) 139, 183, 280, 318, 407
AlwI GGATC 3 cut(s) 215, 530, 543
AoxI GGCC 1 cut(s) 367
ApeKI GCWGC 3 cut(s) 180, 437, 450
ApoI RAATTY 1 cut(s) 11
AspLEI GCGC 1 cut(s) 518
AspS9I GGNCC 1 cut(s) 22
AsuC2I CCSGG 1 cut(s) 447
AsuHPI GGTGA 2 cut(s) 467, 535
AvaII GGWCC 1 cut(s) 22
BamHI GGATCC 1 cut(s) 535
BanI GGYRCC 2 cut(s) 479, 515
BbvI GCAGC 3 cut(s) 192, 449, 462
BccI CCATC 1 cut(s) 452
BcgI CGANNNNNNTGC 2 cut(s) 224, 258
BcnI CCSGG 1 cut(s) 447
BfaI CTAG 1 cut(s) 521
BfmI CTRYAG 2 cut(s) 147, 435
BfoI RGCGCY 1 cut(s) 519
BisI GCNGC 3 cut(s) 181, 438, 451
BlsI GCNGC 3 cut(s) 182, 439, 452
Bme1390I CCNGG 1 cut(s) 447
Bme18I GGWCC 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 22
BmiI GGNNCC 5 cut(s) 23, 232, 481, 517, 537
BmrFI CCNGG 1 cut(s) 447
BmrI ACTGGG 2 cut(s) 63, 280
BmuI ACTGGG 2 cut(s) 63, 280
BpuMI CCSGG 1 cut(s) 447
BsaHI GRCGYC 2 cut(s) 471, 516
BsaJI CCNNGG 2 cut(s) 16, 100
BsaWI WCCGGW 1 cut(s) 24
BsaXI ACNNNNNCTCC 4 cut(s) 302, 332, 391, 421
Bsc4I CCNNNNNNNGG 1 cut(s) 452
Bse1I ACTGG 2 cut(s) 58, 275
BseDI CCNNGG 2 cut(s) 16, 100
BseGI GGATG 2 cut(s) 280, 385
BseLI CCNNNNNNNGG 1 cut(s) 452
BseMII CTCAG 1 cut(s) 514
BseNI ACTGG 2 cut(s) 58, 275
BseXI GCAGC 3 cut(s) 192, 449, 462
BsgI GTGCAG 1 cut(s) 364
BshFI GGCC 1 cut(s) 369
BshNI GGYRCC 2 cut(s) 479, 515
BsiSI CCGG 3 cut(s) 25, 366, 447
BslFI GGGAC 1 cut(s) 35
BslI CCNNNNNNNGG 1 cut(s) 452
BsmFI GGGAC 1 cut(s) 35
BsnI GGCC 1 cut(s) 369
Bsp143I GATC 4 cut(s) 127, 207, 346, 535
BspACI CCGC 3 cut(s) 390, 453, 494
BspANI GGCC 1 cut(s) 369
BspCNI CTCAG 1 cut(s) 513
BspLI GGNNCC 5 cut(s) 23, 232, 481, 517, 537
BspMAI CTGCAG 1 cut(s) 439
BspPI GGATC 3 cut(s) 215, 530, 543
BspT107I GGYRCC 2 cut(s) 479, 515
BsrI ACTGG 2 cut(s) 58, 275
BssECI CCNNGG 2 cut(s) 16, 100
BssMI GATC 4 cut(s) 127, 207, 346, 535
BssNI GRCGYC 2 cut(s) 471, 516
BssT1I CCWWGG 1 cut(s) 16
Bst4CI ACNGT 1 cut(s) 239
BstACI GRCGYC 2 cut(s) 471, 516
BstC8I GCNNGC 1 cut(s) 137
BstDEI CTNAG 1 cut(s) 500
BstF5I GGATG 2 cut(s) 280, 385
BstH2I RGCGCY 1 cut(s) 519
BstHHI GCGC 1 cut(s) 518
BstKTI GATC 4 cut(s) 130, 210, 349, 538
BstMBI GATC 4 cut(s) 127, 207, 346, 535
BstSCI CCNGG 1 cut(s) 445
BstSFI CTRYAG 2 cut(s) 147, 435
BstV1I GCAGC 3 cut(s) 192, 449, 462
BstX2I RGATCY 1 cut(s) 535
BstXI CCANNNNNNTGG 1 cut(s) 532
BstYI RGATCY 1 cut(s) 535
BsuRI GGCC 1 cut(s) 369
BtsCI GGATG 2 cut(s) 280, 385
BtsI GCAGTG 1 cut(s) 175
BtsIMutI CAGTG 4 cut(s) 175, 268, 508, 547
Cac8I GCNNGC 1 cut(s) 137
CfoI GCGC 1 cut(s) 518
Cfr13I GGNCC 1 cut(s) 22
CviAII CATG 2 cut(s) 188, 211
CviJI RGCY 8 cut(s) 135, 139, 183, 280, 318, 369, 407, 524
CviKI_1 RGCY 8 cut(s) 135, 139, 183, 280, 318, 369, 407, 524
DdeI CTNAG 1 cut(s) 500
DinI GGCGCC 1 cut(s) 517
DpnI GATC 4 cut(s) 129, 209, 348, 537
DpnII GATC 4 cut(s) 127, 207, 346, 535
Eco130I CCWWGG 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 22
EcoRI GAATTC 1 cut(s) 11
EcoT14I CCWWGG 1 cut(s) 16
EgeI GGCGCC 1 cut(s) 517
EheI GGCGCC 1 cut(s) 517
ErhI CCWWGG 1 cut(s) 16
FaeI CATG 2 cut(s) 191, 214
FaiI YATR 7 cut(s) 42, 77, 85, 149, 189, 212, 431
FaqI GGGAC 1 cut(s) 35
FatI CATG 2 cut(s) 187, 210
FauI CCCGC 1 cut(s) 397
Fnu4HI GCNGC 3 cut(s) 181, 438, 451
FokI GGATG 2 cut(s) 287, 372
Fsp4HI GCNGC 3 cut(s) 181, 438, 451
FspBI CTAG 1 cut(s) 521
GlaI GCGC 1 cut(s) 517
GluI GCNGC 3 cut(s) 181, 438, 451
HaeII RGCGCY 1 cut(s) 519
HaeIII GGCC 1 cut(s) 369
HapII CCGG 3 cut(s) 25, 366, 447
HhaI GCGC 1 cut(s) 518
Hin1I GRCGYC 2 cut(s) 471, 516
Hin1II CATG 2 cut(s) 191, 214
Hin6I GCGC 1 cut(s) 516
HinP1I GCGC 1 cut(s) 516
HinfI GANTC 5 cut(s) 28, 323, 362, 412, 464
HpaII CCGG 3 cut(s) 25, 366, 447
HphI GGTGA 2 cut(s) 467, 535
Hpy166II GTNNAC 1 cut(s) 301
Hpy188I TCNGA 8 cut(s) 104, 132, 322, 328, 361, 411, 469, 476
Hpy8I GTNNAC 1 cut(s) 301
Hpy99I CGWCG 2 cut(s) 347, 476
HpyAV CCTTC 1 cut(s) 380
HpyCH4III ACNGT 1 cut(s) 239
HpyCH4IV ACGT 2 cut(s) 303, 471
HpyCH4V TGCA 4 cut(s) 245, 257, 381, 437
HpyF3I CTNAG 1 cut(s) 500
HpySE526I ACGT 2 cut(s) 303, 471
Hsp92I GRCGYC 2 cut(s) 471, 516
Hsp92II CATG 2 cut(s) 191, 214
HspAI GCGC 1 cut(s) 516
KasI GGCGCC 1 cut(s) 515
Kzo9I GATC 4 cut(s) 127, 207, 346, 535
LmnI GCTCC 2 cut(s) 323, 412
LpnPI CCDG 9 cut(s) 38, 39, 149, 150, 256, 379, 391, 460, 496
Lsp1109I GCAGC 3 cut(s) 192, 449, 462
MaeI CTAG 1 cut(s) 521
MaeII ACGT 2 cut(s) 303, 471
MalI GATC 4 cut(s) 129, 209, 348, 537
MboI GATC 4 cut(s) 127, 207, 346, 535
MboII GAAGA 1 cut(s) 137
MflI RGATCY 1 cut(s) 535
MluCI AATT 1 cut(s) 11
Mly113I GGCGCC 1 cut(s) 516
MlyI GAGTC 2 cut(s) 332, 421
MmeI TCCRAC 2 cut(s) 351, 454
MnlI CCTC 5 cut(s) 55, 110, 470, 479, 549
MseI TTAA 1 cut(s) 110
MslI CAYNNNNRTG 1 cut(s) 273
MspA1I CMGCKG 2 cut(s) 392, 453
MspI CCGG 3 cut(s) 25, 366, 447
MspR9I CCNGG 1 cut(s) 447
NarI GGCGCC 1 cut(s) 516
NciI CCSGG 1 cut(s) 447
NdeII GATC 4 cut(s) 127, 207, 346, 535
NlaIII CATG 2 cut(s) 191, 214
NlaIV GGNNCC 5 cut(s) 23, 232, 481, 517, 537
PfeI GAWTC 3 cut(s) 28, 362, 464
PkrI GCNGC 3 cut(s) 182, 439, 452
PleI GAGTC 2 cut(s) 331, 420
PluTI GGCGCC 1 cut(s) 519
PpsI GAGTC 2 cut(s) 331, 420
Psp1406I AACGTT 1 cut(s) 303
PspN4I GGNNCC 5 cut(s) 23, 232, 481, 517, 537
PspPI GGNCC 1 cut(s) 22
PsrI GAACNNNNNNTAC 2 cut(s) 293, 325
PstI CTGCAG 1 cut(s) 439
PsuI RGATCY 1 cut(s) 535
RseI CAYNNNNRTG 1 cut(s) 273
SaqAI TTAA 1 cut(s) 110
SatI GCNGC 3 cut(s) 181, 438, 451
Sau3AI GATC 4 cut(s) 127, 207, 346, 535
Sau96I GGNCC 1 cut(s) 22
SchI GAGTC 2 cut(s) 332, 421
ScrFI CCNGG 1 cut(s) 447
SfcI CTRYAG 2 cut(s) 147, 435
SfoI GGCGCC 1 cut(s) 517
SinI GGWCC 1 cut(s) 22
SmiMI CAYNNNNRTG 1 cut(s) 273
Sse9I AATT 1 cut(s) 11
SsiI CCGC 3 cut(s) 390, 453, 494
SspDI GGCGCC 1 cut(s) 515
SspMI CTAG 1 cut(s) 521
StyD4I CCNGG 1 cut(s) 445
StyI CCWWGG 1 cut(s) 16
TaaI ACNGT 1 cut(s) 239
TaiI ACGT 2 cut(s) 306, 474
TasI AATT 1 cut(s) 11
TfiI GAWTC 3 cut(s) 28, 362, 464
Tru1I TTAA 1 cut(s) 110
Tru9I TTAA 1 cut(s) 110
TscAI CASTG 3 cut(s) 175, 275, 508
TseI GCWGC 3 cut(s) 180, 437, 450
TspDTI ATGAA 1 cut(s) 128
TspGWI ACGGA 1 cut(s) 405
TspRI CASTG 3 cut(s) 175, 275, 508
VpaK11BI GGWCC 1 cut(s) 22
XapI RAATTY 1 cut(s) 11
XspI CTAG 1 cut(s) 521
ZraI GACGTC 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.