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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 397 |
| AccBSI | CCGCTC | 1 cut(s) | 85 |
| AciI | CCGC | 1 cut(s) | 85 |
| AclWI | GGATC | 1 cut(s) | 335 |
| AfaI | GTAC | 2 cut(s) | 38, 307 |
| AflIII | ACRYGT | 1 cut(s) | 39 |
| AgsI | TTSAA | 3 cut(s) | 124, 131, 160 |
| AluBI | AGCT | 5 cut(s) | 64, 188, 244, 346, 368 |
| AluI | AGCT | 5 cut(s) | 64, 188, 244, 346, 368 |
| Alw26I | GTCTC | 1 cut(s) | 295 |
| AlwI | GGATC | 1 cut(s) | 335 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 189 |
| AspLEI | GCGC | 1 cut(s) | 147 |
| BauI | CACGAG | 1 cut(s) | 331 |
| BccI | CCATC | 2 cut(s) | 236, 318 |
| BcoDI | GTCTC | 1 cut(s) | 295 |
| BfaI | CTAG | 1 cut(s) | 77 |
| BmsI | GCATC | 1 cut(s) | 43 |
| BsaI | GGTCTC | 1 cut(s) | 295 |
| BsaJI | CCNNGG | 1 cut(s) | 309 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 26, 56 |
| Bse3DI | GCAATG | 1 cut(s) | 100 |
| BseDI | CCNNGG | 1 cut(s) | 309 |
| BseGI | GGATG | 1 cut(s) | 329 |
| BseMI | GCAATG | 1 cut(s) | 100 |
| BseMII | CTCAG | 2 cut(s) | 40, 360 |
| BseRI | GAGGAG | 1 cut(s) | 101 |
| BsmAI | GTCTC | 1 cut(s) | 295 |
| Bso31I | GGTCTC | 1 cut(s) | 295 |
| Bsp143I | GATC | 1 cut(s) | 327 |
| BspACI | CCGC | 1 cut(s) | 85 |
| BspCNI | CTCAG | 2 cut(s) | 39, 361 |
| BspPI | GGATC | 1 cut(s) | 335 |
| BspQI | GCTCTTC | 2 cut(s) | 75, 195 |
| BspTNI | GGTCTC | 1 cut(s) | 295 |
| BsrBI | CCGCTC | 1 cut(s) | 85 |
| BsrDI | GCAATG | 1 cut(s) | 100 |
| BssECI | CCNNGG | 1 cut(s) | 309 |
| BssMI | GATC | 1 cut(s) | 327 |
| BssSI | CACGAG | 1 cut(s) | 331 |
| Bst2BI | CACGAG | 1 cut(s) | 331 |
| Bst6I | CTCTTC | 2 cut(s) | 75, 195 |
| BstDEI | CTNAG | 2 cut(s) | 26, 369 |
| BstF5I | GGATG | 1 cut(s) | 329 |
| BstHHI | GCGC | 1 cut(s) | 147 |
| BstKTI | GATC | 1 cut(s) | 330 |
| BstMAI | GTCTC | 1 cut(s) | 295 |
| BstMBI | GATC | 1 cut(s) | 327 |
| BstNSI | RCATGY | 1 cut(s) | 43 |
| BtsCI | GGATG | 1 cut(s) | 329 |
| CfoI | GCGC | 1 cut(s) | 147 |
| Csp6I | GTAC | 2 cut(s) | 37, 306 |
| CviAII | CATG | 2 cut(s) | 40, 377 |
| CviJI | RGCY | 5 cut(s) | 64, 188, 244, 346, 368 |
| CviKI_1 | RGCY | 5 cut(s) | 64, 188, 244, 346, 368 |
| CviQI | GTAC | 2 cut(s) | 37, 306 |
| DdeI | CTNAG | 2 cut(s) | 26, 369 |
| DpnI | GATC | 1 cut(s) | 329 |
| DpnII | GATC | 1 cut(s) | 327 |
| Eam1104I | CTCTTC | 2 cut(s) | 75, 195 |
| EarI | CTCTTC | 2 cut(s) | 75, 195 |
| Eco31I | GGTCTC | 1 cut(s) | 295 |
| FaeI | CATG | 2 cut(s) | 43, 380 |
| FatI | CATG | 2 cut(s) | 39, 376 |
| FokI | GGATG | 1 cut(s) | 336 |
| FspBI | CTAG | 1 cut(s) | 77 |
| GlaI | GCGC | 1 cut(s) | 146 |
| HhaI | GCGC | 1 cut(s) | 147 |
| Hin1II | CATG | 2 cut(s) | 43, 380 |
| Hin6I | GCGC | 1 cut(s) | 145 |
| HinP1I | GCGC | 1 cut(s) | 145 |
| HinfI | GANTC | 1 cut(s) | 104 |
| Hpy166II | GTNNAC | 2 cut(s) | 197, 221 |
| Hpy188I | TCNGA | 2 cut(s) | 29, 184 |
| Hpy188III | TCNNGA | 1 cut(s) | 331 |
| Hpy8I | GTNNAC | 2 cut(s) | 197, 221 |
| HpyAV | CCTTC | 2 cut(s) | 118, 277 |
| HpyCH4V | TGCA | 1 cut(s) | 56 |
| HpyF3I | CTNAG | 2 cut(s) | 26, 369 |
| Hsp92II | CATG | 2 cut(s) | 43, 380 |
| HspAI | GCGC | 1 cut(s) | 145 |
| Kzo9I | GATC | 1 cut(s) | 327 |
| LguI | GCTCTTC | 2 cut(s) | 75, 195 |
| LpnPI | CCDG | 1 cut(s) | 129 |
| LweI | GCATC | 1 cut(s) | 43 |
| MaeI | CTAG | 1 cut(s) | 77 |
| MalI | GATC | 1 cut(s) | 329 |
| MbiI | CCGCTC | 1 cut(s) | 85 |
| MboI | GATC | 1 cut(s) | 327 |
| MboII | GAAGA | 2 cut(s) | 92, 182 |
| MluCI | AATT | 4 cut(s) | 178, 246, 282, 392 |
| MmeI | TCCRAC | 1 cut(s) | 301 |
| MnlI | CCTC | 4 cut(s) | 119, 122, 304, 327 |
| MroXI | GAANNNNTTC | 1 cut(s) | 189 |
| NdeII | GATC | 1 cut(s) | 327 |
| NlaIII | CATG | 2 cut(s) | 43, 380 |
| NspI | RCATGY | 1 cut(s) | 43 |
| PciI | ACATGT | 1 cut(s) | 39 |
| PciSI | GCTCTTC | 2 cut(s) | 75, 195 |
| PdmI | GAANNNNTTC | 1 cut(s) | 189 |
| PfeI | GAWTC | 1 cut(s) | 104 |
| PscI | ACATGT | 1 cut(s) | 39 |
| PsiI | TTATAA | 1 cut(s) | 397 |
| RsaI | GTAC | 2 cut(s) | 38, 307 |
| RsaNI | GTAC | 2 cut(s) | 37, 306 |
| SapI | GCTCTTC | 2 cut(s) | 75, 195 |
| Sau3AI | GATC | 1 cut(s) | 327 |
| SetI | ASST | 9 cut(s) | 66, 129, 190, 246, 269, 315, 338, 348, 370 |
| SfaNI | GCATC | 1 cut(s) | 43 |
| Sse9I | AATT | 4 cut(s) | 178, 246, 282, 392 |
| SsiI | CCGC | 1 cut(s) | 85 |
| SspI | AATATT | 1 cut(s) | 302 |
| SspMI | CTAG | 1 cut(s) | 77 |
| TasI | AATT | 4 cut(s) | 178, 246, 282, 392 |
| TatI | WGTACW | 1 cut(s) | 36 |
| TfiI | GAWTC | 1 cut(s) | 104 |
| TspDTI | ATGAA | 1 cut(s) | 329 |
| TspGWI | ACGGA | 1 cut(s) | 154 |
| XceI | RCATGY | 1 cut(s) | 43 |
| XmnI | GAANNNNTTC | 1 cut(s) | 189 |
| XspI | CTAG | 1 cut(s) | 77 |