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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfaI | GTAC | 1 cut(s) | 268 |
| AfeI | AGCGCT | 2 cut(s) | 36, 47 |
| AgsI | TTSAA | 3 cut(s) | 85, 92, 121 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 121, 188 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 33, 65 |
| AluBI | AGCT | 3 cut(s) | 25, 149, 205 |
| AluI | AGCT | 3 cut(s) | 25, 149, 205 |
| Aor51HI | AGCGCT | 2 cut(s) | 36, 47 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 150 |
| AspLEI | GCGC | 2 cut(s) | 37, 48 |
| BfaI | CTAG | 1 cut(s) | 38 |
| BfoI | RGCGCY | 2 cut(s) | 38, 49 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 121, 188 |
| BmsI | GCATC | 1 cut(s) | 4 |
| Bse3DI | GCAATG | 1 cut(s) | 61 |
| BseGI | GGATG | 1 cut(s) | 19 |
| BseMI | GCAATG | 1 cut(s) | 61 |
| BseRI | GAGGAG | 1 cut(s) | 62 |
| Bsp143I | GATC | 1 cut(s) | 6 |
| BspQI | GCTCTTC | 2 cut(s) | 36, 156 |
| BsrDI | GCAATG | 1 cut(s) | 61 |
| BssMI | GATC | 1 cut(s) | 6 |
| Bst6I | CTCTTC | 2 cut(s) | 36, 156 |
| BstDEI | CTNAG | 1 cut(s) | 270 |
| BstF5I | GGATG | 1 cut(s) | 19 |
| BstH2I | RGCGCY | 2 cut(s) | 38, 49 |
| BstHHI | GCGC | 2 cut(s) | 37, 48 |
| BstKTI | GATC | 1 cut(s) | 9 |
| BstMBI | GATC | 1 cut(s) | 6 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 43 |
| BtsCI | GGATG | 1 cut(s) | 19 |
| CfoI | GCGC | 2 cut(s) | 37, 48 |
| Csp6I | GTAC | 1 cut(s) | 267 |
| CviAII | CATG | 1 cut(s) | 166 |
| CviJI | RGCY | 4 cut(s) | 25, 149, 205, 242 |
| CviKI_1 | RGCY | 4 cut(s) | 25, 149, 205, 242 |
| CviQI | GTAC | 1 cut(s) | 267 |
| DdeI | CTNAG | 1 cut(s) | 270 |
| DpnI | GATC | 1 cut(s) | 8 |
| DpnII | GATC | 1 cut(s) | 6 |
| DriI | GACNNNNNGTC | 2 cut(s) | 121, 188 |
| Eam1104I | CTCTTC | 2 cut(s) | 36, 156 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 121, 188 |
| EarI | CTCTTC | 2 cut(s) | 36, 156 |
| Eco47III | AGCGCT | 2 cut(s) | 36, 47 |
| FaeI | CATG | 1 cut(s) | 169 |
| FaiI | YATR | 4 cut(s) | 28, 138, 167, 196 |
| FatI | CATG | 1 cut(s) | 165 |
| FokI | GGATG | 1 cut(s) | 26 |
| FspBI | CTAG | 1 cut(s) | 38 |
| GlaI | GCGC | 2 cut(s) | 36, 47 |
| HaeII | RGCGCY | 2 cut(s) | 38, 49 |
| HhaI | GCGC | 2 cut(s) | 37, 48 |
| Hin1II | CATG | 1 cut(s) | 169 |
| Hin6I | GCGC | 2 cut(s) | 35, 46 |
| HinP1I | GCGC | 2 cut(s) | 35, 46 |
| HinfI | GANTC | 1 cut(s) | 65 |
| Hpy166II | GTNNAC | 1 cut(s) | 182 |
| Hpy188III | TCNNGA | 1 cut(s) | 200 |
| Hpy8I | GTNNAC | 1 cut(s) | 182 |
| HpyAV | CCTTC | 2 cut(s) | 79, 238 |
| HpyCH4V | TGCA | 1 cut(s) | 17 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 43 |
| HpyF3I | CTNAG | 1 cut(s) | 270 |
| Hsp92II | CATG | 1 cut(s) | 169 |
| HspAI | GCGC | 2 cut(s) | 35, 46 |
| Kzo9I | GATC | 1 cut(s) | 6 |
| LguI | GCTCTTC | 2 cut(s) | 36, 156 |
| LpnPI | CCDG | 2 cut(s) | 90, 267 |
| LweI | GCATC | 1 cut(s) | 4 |
| MaeI | CTAG | 1 cut(s) | 38 |
| MaeIII | GTNAC | 2 cut(s) | 127, 190 |
| MalI | GATC | 1 cut(s) | 8 |
| MboI | GATC | 1 cut(s) | 6 |
| MboII | GAAGA | 2 cut(s) | 53, 143 |
| MluCI | AATT | 2 cut(s) | 139, 207 |
| MnlI | CCTC | 2 cut(s) | 80, 83 |
| MroXI | GAANNNNTTC | 1 cut(s) | 150 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 43 |
| NdeII | GATC | 1 cut(s) | 6 |
| NlaIII | CATG | 1 cut(s) | 169 |
| NmuCI | GTSAC | 1 cut(s) | 190 |
| PciSI | GCTCTTC | 2 cut(s) | 36, 156 |
| PdmI | GAANNNNTTC | 1 cut(s) | 150 |
| PfeI | GAWTC | 1 cut(s) | 65 |
| RsaI | GTAC | 1 cut(s) | 268 |
| RsaNI | GTAC | 1 cut(s) | 267 |
| SapI | GCTCTTC | 2 cut(s) | 36, 156 |
| Sau3AI | GATC | 1 cut(s) | 6 |
| SetI | ASST | 6 cut(s) | 27, 90, 151, 207, 230, 276 |
| SfaNI | GCATC | 1 cut(s) | 4 |
| Sse9I | AATT | 2 cut(s) | 139, 207 |
| SspMI | CTAG | 1 cut(s) | 38 |
| TaqI | TCGA | 1 cut(s) | 5 |
| TasI | AATT | 2 cut(s) | 139, 207 |
| TfiI | GAWTC | 1 cut(s) | 65 |
| TseFI | GTSAC | 1 cut(s) | 190 |
| Tsp45I | GTSAC | 1 cut(s) | 190 |
| XmnI | GAANNNNTTC | 1 cut(s) | 150 |
| XspI | CTAG | 1 cut(s) | 38 |