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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 296 |
| AfaI | GTAC | 1 cut(s) | 268 |
| AfeI | AGCGCT | 2 cut(s) | 36, 47 |
| AgsI | TTSAA | 3 cut(s) | 85, 92, 121 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 121, 188 |
| AjnI | CCWGG | 1 cut(s) | 231 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 33, 65 |
| AluBI | AGCT | 4 cut(s) | 25, 205, 307, 329 |
| AluI | AGCT | 4 cut(s) | 25, 205, 307, 329 |
| AlwI | GGATC | 1 cut(s) | 296 |
| Aor51HI | AGCGCT | 2 cut(s) | 36, 47 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 150 |
| AspLEI | GCGC | 3 cut(s) | 37, 48, 108 |
| BauI | CACGAG | 1 cut(s) | 292 |
| BciT130I | CCWGG | 1 cut(s) | 233 |
| BfaI | CTAG | 1 cut(s) | 38 |
| BfmI | CTRYAG | 1 cut(s) | 324 |
| BfoI | RGCGCY | 2 cut(s) | 38, 49 |
| Bme1390I | CCNGG | 1 cut(s) | 233 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 121, 188 |
| BmrFI | CCNGG | 1 cut(s) | 233 |
| BmsI | GCATC | 1 cut(s) | 4 |
| Bse3DI | GCAATG | 1 cut(s) | 61 |
| BseBI | CCWGG | 1 cut(s) | 233 |
| BseGI | GGATG | 2 cut(s) | 72, 239 |
| BseMI | GCAATG | 1 cut(s) | 61 |
| BseMII | CTCAG | 2 cut(s) | 261, 321 |
| Bsp143I | GATC | 2 cut(s) | 6, 288 |
| BspCNI | CTCAG | 2 cut(s) | 262, 322 |
| BspPI | GGATC | 1 cut(s) | 296 |
| BsrDI | GCAATG | 1 cut(s) | 61 |
| BssMI | GATC | 2 cut(s) | 6, 288 |
| BssSI | CACGAG | 1 cut(s) | 292 |
| Bst2BI | CACGAG | 1 cut(s) | 292 |
| Bst2UI | CCWGG | 1 cut(s) | 233 |
| Bst6I | CTCTTC | 1 cut(s) | 156 |
| BstDEI | CTNAG | 2 cut(s) | 270, 330 |
| BstF5I | GGATG | 2 cut(s) | 72, 239 |
| BstH2I | RGCGCY | 2 cut(s) | 38, 49 |
| BstHHI | GCGC | 3 cut(s) | 37, 48, 108 |
| BstKTI | GATC | 2 cut(s) | 9, 291 |
| BstMBI | GATC | 2 cut(s) | 6, 288 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 43 |
| BstNI | CCWGG | 1 cut(s) | 233 |
| BstSCI | CCNGG | 1 cut(s) | 231 |
| BstSFI | CTRYAG | 1 cut(s) | 324 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 232 |
| BtsCI | GGATG | 2 cut(s) | 72, 239 |
| CfoI | GCGC | 3 cut(s) | 37, 48, 108 |
| Csp6I | GTAC | 1 cut(s) | 267 |
| CviAII | CATG | 1 cut(s) | 338 |
| CviJI | RGCY | 4 cut(s) | 25, 205, 307, 329 |
| CviKI_1 | RGCY | 4 cut(s) | 25, 205, 307, 329 |
| CviQI | GTAC | 1 cut(s) | 267 |
| DdeI | CTNAG | 2 cut(s) | 270, 330 |
| DpnI | GATC | 2 cut(s) | 8, 290 |
| DpnII | GATC | 2 cut(s) | 6, 288 |
| DriI | GACNNNNNGTC | 2 cut(s) | 121, 188 |
| Eam1104I | CTCTTC | 1 cut(s) | 156 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 121, 188 |
| EarI | CTCTTC | 1 cut(s) | 156 |
| Eco47III | AGCGCT | 2 cut(s) | 36, 47 |
| EcoRII | CCWGG | 1 cut(s) | 231 |
| FaeI | CATG | 1 cut(s) | 341 |
| FaiI | YATR | 4 cut(s) | 28, 138, 320, 339 |
| FatI | CATG | 1 cut(s) | 337 |
| FokI | GGATG | 2 cut(s) | 59, 226 |
| FspBI | CTAG | 1 cut(s) | 38 |
| GlaI | GCGC | 3 cut(s) | 36, 47, 107 |
| HaeII | RGCGCY | 2 cut(s) | 38, 49 |
| HhaI | GCGC | 3 cut(s) | 37, 48, 108 |
| Hin1II | CATG | 1 cut(s) | 341 |
| Hin6I | GCGC | 3 cut(s) | 35, 46, 106 |
| HinP1I | GCGC | 3 cut(s) | 35, 46, 106 |
| HinfI | GANTC | 2 cut(s) | 65, 146 |
| Hpy166II | GTNNAC | 1 cut(s) | 182 |
| Hpy188I | TCNGA | 1 cut(s) | 145 |
| Hpy188III | TCNNGA | 1 cut(s) | 292 |
| Hpy8I | GTNNAC | 1 cut(s) | 182 |
| HpyAV | CCTTC | 2 cut(s) | 79, 238 |
| HpyCH4V | TGCA | 1 cut(s) | 17 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 43 |
| HpyF3I | CTNAG | 2 cut(s) | 270, 330 |
| Hsp92II | CATG | 1 cut(s) | 341 |
| HspAI | GCGC | 3 cut(s) | 35, 46, 106 |
| Kzo9I | GATC | 2 cut(s) | 6, 288 |
| LpnPI | CCDG | 5 cut(s) | 90, 201, 218, 245, 267 |
| LweI | GCATC | 1 cut(s) | 4 |
| MaeI | CTAG | 1 cut(s) | 38 |
| MaeIII | GTNAC | 1 cut(s) | 190 |
| MalI | GATC | 2 cut(s) | 8, 290 |
| MboI | GATC | 2 cut(s) | 6, 288 |
| MboII | GAAGA | 1 cut(s) | 143 |
| MluCI | AATT | 2 cut(s) | 139, 207 |
| MnlI | CCTC | 3 cut(s) | 80, 265, 288 |
| MroXI | GAANNNNTTC | 1 cut(s) | 150 |
| MspR9I | CCNGG | 1 cut(s) | 233 |
| MvaI | CCWGG | 1 cut(s) | 233 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 43 |
| NdeII | GATC | 2 cut(s) | 6, 288 |
| NlaIII | CATG | 1 cut(s) | 341 |
| NmuCI | GTSAC | 1 cut(s) | 190 |
| PdmI | GAANNNNTTC | 1 cut(s) | 150 |
| PfeI | GAWTC | 2 cut(s) | 65, 146 |
| Psp6I | CCWGG | 1 cut(s) | 231 |
| PspGI | CCWGG | 1 cut(s) | 231 |
| RsaI | GTAC | 1 cut(s) | 268 |
| RsaNI | GTAC | 1 cut(s) | 267 |
| Sau3AI | GATC | 2 cut(s) | 6, 288 |
| ScrFI | CCNGG | 1 cut(s) | 233 |
| SetI | ASST | 9 cut(s) | 27, 90, 190, 207, 230, 276, 299, 309, 331 |
| SfaNI | GCATC | 1 cut(s) | 4 |
| SfcI | CTRYAG | 1 cut(s) | 324 |
| Sse9I | AATT | 2 cut(s) | 139, 207 |
| SspMI | CTAG | 1 cut(s) | 38 |
| StyD4I | CCNGG | 1 cut(s) | 231 |
| TaqI | TCGA | 1 cut(s) | 5 |
| TasI | AATT | 2 cut(s) | 139, 207 |
| TfiI | GAWTC | 2 cut(s) | 65, 146 |
| TseFI | GTSAC | 1 cut(s) | 190 |
| Tsp45I | GTSAC | 1 cut(s) | 190 |
| TspDTI | ATGAA | 2 cut(s) | 228, 290 |
| TspGWI | ACGGA | 1 cut(s) | 301 |
| XmnI | GAANNNNTTC | 1 cut(s) | 150 |
| XspI | CTAG | 1 cut(s) | 38 |