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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 152 |
| AclWI | GGATC | 1 cut(s) | 335 |
| AfeI | AGCGCT | 1 cut(s) | 75 |
| AflIII | ACRYGT | 2 cut(s) | 39, 145 |
| AgsI | TTSAA | 1 cut(s) | 160 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 227 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 72, 104 |
| AluBI | AGCT | 1 cut(s) | 244 |
| AluI | AGCT | 1 cut(s) | 244 |
| AlwI | GGATC | 1 cut(s) | 335 |
| Aor51HI | AGCGCT | 1 cut(s) | 75 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 189 |
| AspLEI | GCGC | 1 cut(s) | 76 |
| AspS9I | GGNCC | 1 cut(s) | 127 |
| AsuHPI | GGTGA | 1 cut(s) | 256 |
| AvaII | GGWCC | 1 cut(s) | 127 |
| BauI | CACGAG | 1 cut(s) | 331 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 294, 299, 328, 333 |
| BfaI | CTAG | 1 cut(s) | 77 |
| BfoI | RGCGCY | 1 cut(s) | 77 |
| BglII | AGATCT | 1 cut(s) | 187 |
| Bme18I | GGWCC | 1 cut(s) | 127 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 227 |
| BmgT120I | GGNCC | 1 cut(s) | 127 |
| BmsI | GCATC | 1 cut(s) | 43 |
| BsaAI | YACGTR | 1 cut(s) | 146 |
| Bse3DI | GCAATG | 2 cut(s) | 100, 322 |
| BseMI | GCAATG | 2 cut(s) | 100, 322 |
| BseRI | GAGGAG | 1 cut(s) | 101 |
| BsmI | GAATGC | 1 cut(s) | 317 |
| Bsp143I | GATC | 2 cut(s) | 187, 327 |
| BspACI | CCGC | 1 cut(s) | 152 |
| BspPI | GGATC | 1 cut(s) | 335 |
| BsrDI | GCAATG | 2 cut(s) | 100, 322 |
| BssMI | GATC | 2 cut(s) | 187, 327 |
| BssSI | CACGAG | 1 cut(s) | 331 |
| Bst2BI | CACGAG | 1 cut(s) | 331 |
| Bst4CI | ACNGT | 1 cut(s) | 166 |
| Bst6I | CTCTTC | 2 cut(s) | 75, 266 |
| BstBAI | YACGTR | 1 cut(s) | 146 |
| BstDEI | CTNAG | 1 cut(s) | 277 |
| BstH2I | RGCGCY | 1 cut(s) | 77 |
| BstHHI | GCGC | 1 cut(s) | 76 |
| BstKTI | GATC | 2 cut(s) | 190, 330 |
| BstMBI | GATC | 2 cut(s) | 187, 327 |
| BstNSI | RCATGY | 1 cut(s) | 43 |
| BstX2I | RGATCY | 1 cut(s) | 187 |
| BstYI | RGATCY | 1 cut(s) | 187 |
| CfoI | GCGC | 1 cut(s) | 76 |
| Cfr13I | GGNCC | 1 cut(s) | 127 |
| CviAII | CATG | 3 cut(s) | 13, 40, 205 |
| CviJI | RGCY | 2 cut(s) | 226, 244 |
| CviKI_1 | RGCY | 2 cut(s) | 226, 244 |
| DdeI | CTNAG | 1 cut(s) | 277 |
| DpnI | GATC | 2 cut(s) | 189, 329 |
| DpnII | GATC | 2 cut(s) | 187, 327 |
| DraI | TTTAAA | 1 cut(s) | 123 |
| DriI | GACNNNNNGTC | 1 cut(s) | 227 |
| Eam1104I | CTCTTC | 2 cut(s) | 75, 266 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 227 |
| EarI | CTCTTC | 2 cut(s) | 75, 266 |
| Eco47I | GGWCC | 1 cut(s) | 127 |
| Eco47III | AGCGCT | 1 cut(s) | 75 |
| FaeI | CATG | 3 cut(s) | 16, 43, 208 |
| FaiI | YATR | 5 cut(s) | 14, 41, 67, 177, 206 |
| FatI | CATG | 3 cut(s) | 12, 39, 204 |
| FspBI | CTAG | 1 cut(s) | 77 |
| GlaI | GCGC | 1 cut(s) | 75 |
| HaeII | RGCGCY | 1 cut(s) | 77 |
| HhaI | GCGC | 1 cut(s) | 76 |
| Hin1II | CATG | 3 cut(s) | 16, 43, 208 |
| Hin6I | GCGC | 1 cut(s) | 74 |
| HinP1I | GCGC | 1 cut(s) | 74 |
| HinfI | GANTC | 1 cut(s) | 104 |
| HphI | GGTGA | 1 cut(s) | 256 |
| Hpy166II | GTNNAC | 1 cut(s) | 221 |
| Hpy188I | TCNGA | 1 cut(s) | 184 |
| Hpy188III | TCNNGA | 1 cut(s) | 331 |
| Hpy8I | GTNNAC | 1 cut(s) | 221 |
| Hpy99I | CGWCG | 1 cut(s) | 9 |
| HpyAV | CCTTC | 1 cut(s) | 277 |
| HpyCH4III | ACNGT | 1 cut(s) | 166 |
| HpyCH4IV | ACGT | 1 cut(s) | 145 |
| HpyCH4V | TGCA | 2 cut(s) | 56, 315 |
| HpyF3I | CTNAG | 1 cut(s) | 277 |
| HpySE526I | ACGT | 1 cut(s) | 145 |
| Hsp92II | CATG | 3 cut(s) | 16, 43, 208 |
| HspAI | GCGC | 1 cut(s) | 74 |
| Kzo9I | GATC | 2 cut(s) | 187, 327 |
| LmnI | GCTCC | 1 cut(s) | 135 |
| LpnPI | CCDG | 4 cut(s) | 129, 240, 285, 306 |
| LweI | GCATC | 1 cut(s) | 43 |
| MaeI | CTAG | 1 cut(s) | 77 |
| MaeII | ACGT | 1 cut(s) | 145 |
| MaeIII | GTNAC | 1 cut(s) | 229 |
| MalI | GATC | 2 cut(s) | 189, 329 |
| MboI | GATC | 2 cut(s) | 187, 327 |
| MboII | GAAGA | 4 cut(s) | 92, 182, 197, 253 |
| MflI | RGATCY | 1 cut(s) | 187 |
| MluCI | AATT | 4 cut(s) | 62, 178, 246, 340 |
| MnlI | CCTC | 3 cut(s) | 122, 206, 327 |
| MroXI | GAANNNNTTC | 1 cut(s) | 189 |
| MseI | TTAA | 2 cut(s) | 122, 339 |
| MslI | CAYNNNNRTG | 1 cut(s) | 209 |
| Mva1269I | GAATGC | 1 cut(s) | 317 |
| NdeII | GATC | 2 cut(s) | 187, 327 |
| NlaIII | CATG | 3 cut(s) | 16, 43, 208 |
| NmuCI | GTSAC | 1 cut(s) | 229 |
| NspI | RCATGY | 1 cut(s) | 43 |
| PciI | ACATGT | 1 cut(s) | 39 |
| PctI | GAATGC | 1 cut(s) | 317 |
| PdmI | GAANNNNTTC | 1 cut(s) | 189 |
| PfeI | GAWTC | 1 cut(s) | 104 |
| Ppu21I | YACGTR | 1 cut(s) | 146 |
| PscI | ACATGT | 1 cut(s) | 39 |
| PspPI | GGNCC | 1 cut(s) | 127 |
| PsuI | RGATCY | 1 cut(s) | 187 |
| RseI | CAYNNNNRTG | 1 cut(s) | 209 |
| SaqAI | TTAA | 2 cut(s) | 122, 339 |
| Sau3AI | GATC | 2 cut(s) | 187, 327 |
| Sau96I | GGNCC | 1 cut(s) | 127 |
| SetI | ASST | 5 cut(s) | 129, 148, 246, 269, 338 |
| SfaNI | GCATC | 1 cut(s) | 43 |
| SinI | GGWCC | 1 cut(s) | 127 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 209 |
| Sse9I | AATT | 4 cut(s) | 62, 178, 246, 340 |
| SsiI | CCGC | 1 cut(s) | 152 |
| SspMI | CTAG | 1 cut(s) | 77 |
| TaaI | ACNGT | 1 cut(s) | 166 |
| TaiI | ACGT | 1 cut(s) | 148 |
| TaqI | TCGA | 2 cut(s) | 44, 304 |
| TasI | AATT | 4 cut(s) | 62, 178, 246, 340 |
| TfiI | GAWTC | 1 cut(s) | 104 |
| Tru1I | TTAA | 2 cut(s) | 122, 339 |
| Tru9I | TTAA | 2 cut(s) | 122, 339 |
| TseFI | GTSAC | 1 cut(s) | 229 |
| Tsp45I | GTSAC | 1 cut(s) | 229 |
| TspGWI | ACGGA | 1 cut(s) | 340 |
| VpaK11BI | GGWCC | 1 cut(s) | 127 |
| XceI | RCATGY | 1 cut(s) | 43 |
| XmnI | GAANNNNTTC | 1 cut(s) | 189 |
| XspI | CTAG | 1 cut(s) | 77 |