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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 335 |
| AcsI | RAATTY | 1 cut(s) | 200 |
| AfaI | GTAC | 1 cut(s) | 38 |
| AgsI | TTSAA | 2 cut(s) | 124, 160 |
| AjiI | CACGTC | 1 cut(s) | 274 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 72, 104 |
| AluBI | AGCT | 2 cut(s) | 244, 350 |
| AluI | AGCT | 2 cut(s) | 244, 350 |
| Alw26I | GTCTC | 2 cut(s) | 172, 263 |
| AlwI | GGATC | 1 cut(s) | 335 |
| ApoI | RAATTY | 1 cut(s) | 200 |
| AsuHPI | GGTGA | 2 cut(s) | 142, 320 |
| BauI | CACGAG | 1 cut(s) | 335 |
| BccI | CCATC | 1 cut(s) | 236 |
| BcoDI | GTCTC | 2 cut(s) | 172, 263 |
| BfaI | CTAG | 2 cut(s) | 77, 287 |
| BmgBI | CACGTC | 1 cut(s) | 274 |
| BmsI | GCATC | 1 cut(s) | 43 |
| Bsa29I | ATCGAT | 1 cut(s) | 330 |
| BseCI | ATCGAT | 1 cut(s) | 330 |
| BseGI | GGATG | 2 cut(s) | 58, 96 |
| BseMII | CTCAG | 1 cut(s) | 40 |
| BseRI | GAGGAG | 1 cut(s) | 101 |
| BshVI | ATCGAT | 1 cut(s) | 330 |
| BsmAI | GTCTC | 2 cut(s) | 172, 263 |
| BsmI | GAATGC | 1 cut(s) | 89 |
| Bsp143I | GATC | 3 cut(s) | 45, 327, 331 |
| BspCNI | CTCAG | 1 cut(s) | 39 |
| BspDI | ATCGAT | 1 cut(s) | 330 |
| BspPI | GGATC | 1 cut(s) | 335 |
| BssMI | GATC | 3 cut(s) | 45, 327, 331 |
| BssSI | CACGAG | 1 cut(s) | 335 |
| Bst2BI | CACGAG | 1 cut(s) | 335 |
| Bst4CI | ACNGT | 1 cut(s) | 166 |
| Bst6I | CTCTTC | 1 cut(s) | 248 |
| BstDEI | CTNAG | 2 cut(s) | 26, 277 |
| BstF5I | GGATG | 2 cut(s) | 58, 96 |
| BstKTI | GATC | 3 cut(s) | 48, 330, 334 |
| BstMAI | GTCTC | 2 cut(s) | 172, 263 |
| BstMBI | GATC | 3 cut(s) | 45, 327, 331 |
| Bsu15I | ATCGAT | 1 cut(s) | 330 |
| BsuTUI | ATCGAT | 1 cut(s) | 330 |
| BtgZI | GCGATG | 1 cut(s) | 19 |
| BtrI | CACGTC | 1 cut(s) | 274 |
| BtsCI | GGATG | 2 cut(s) | 58, 96 |
| ClaI | ATCGAT | 1 cut(s) | 330 |
| Csp6I | GTAC | 1 cut(s) | 37 |
| CviAII | CATG | 2 cut(s) | 13, 40 |
| CviJI | RGCY | 3 cut(s) | 227, 244, 350 |
| CviKI_1 | RGCY | 3 cut(s) | 227, 244, 350 |
| CviQI | GTAC | 1 cut(s) | 37 |
| DdeI | CTNAG | 2 cut(s) | 26, 277 |
| DpnI | GATC | 3 cut(s) | 47, 329, 333 |
| DpnII | GATC | 3 cut(s) | 45, 327, 331 |
| Eam1104I | CTCTTC | 1 cut(s) | 248 |
| EarI | CTCTTC | 1 cut(s) | 248 |
| FaeI | CATG | 2 cut(s) | 16, 43 |
| FaiI | YATR | 5 cut(s) | 14, 41, 67, 233, 303 |
| FatI | CATG | 2 cut(s) | 12, 39 |
| FokI | GGATG | 2 cut(s) | 65, 83 |
| FspBI | CTAG | 2 cut(s) | 77, 287 |
| Hin1II | CATG | 2 cut(s) | 16, 43 |
| HinfI | GANTC | 3 cut(s) | 104, 185, 256 |
| HphI | GGTGA | 2 cut(s) | 142, 320 |
| Hpy166II | GTNNAC | 1 cut(s) | 221 |
| Hpy188I | TCNGA | 2 cut(s) | 29, 172 |
| Hpy188III | TCNNGA | 2 cut(s) | 160, 335 |
| Hpy8I | GTNNAC | 1 cut(s) | 221 |
| HpyAV | CCTTC | 2 cut(s) | 118, 277 |
| HpyCH4III | ACNGT | 1 cut(s) | 166 |
| HpyCH4IV | ACGT | 1 cut(s) | 273 |
| HpyCH4V | TGCA | 1 cut(s) | 56 |
| HpyF3I | CTNAG | 2 cut(s) | 26, 277 |
| HpySE526I | ACGT | 1 cut(s) | 273 |
| Hsp92II | CATG | 2 cut(s) | 16, 43 |
| Kzo9I | GATC | 3 cut(s) | 45, 327, 331 |
| LpnPI | CCDG | 2 cut(s) | 129, 306 |
| LweI | GCATC | 1 cut(s) | 43 |
| MaeI | CTAG | 2 cut(s) | 77, 287 |
| MaeII | ACGT | 1 cut(s) | 273 |
| MaeIII | GTNAC | 1 cut(s) | 92 |
| MalI | GATC | 3 cut(s) | 47, 329, 333 |
| MboI | GATC | 3 cut(s) | 45, 327, 331 |
| MboII | GAAGA | 2 cut(s) | 92, 265 |
| MluCI | AATT | 4 cut(s) | 178, 200, 246, 352 |
| MlyI | GAGTC | 1 cut(s) | 265 |
| MnlI | CCTC | 5 cut(s) | 119, 122, 204, 206, 331 |
| MseI | TTAA | 2 cut(s) | 129, 355 |
| Mva1269I | GAATGC | 1 cut(s) | 89 |
| NdeII | GATC | 3 cut(s) | 45, 327, 331 |
| NlaIII | CATG | 2 cut(s) | 16, 43 |
| PctI | GAATGC | 1 cut(s) | 89 |
| PfeI | GAWTC | 2 cut(s) | 104, 185 |
| PleI | GAGTC | 1 cut(s) | 264 |
| PpsI | GAGTC | 1 cut(s) | 264 |
| RsaI | GTAC | 1 cut(s) | 38 |
| RsaNI | GTAC | 1 cut(s) | 37 |
| SaqAI | TTAA | 2 cut(s) | 129, 355 |
| Sau3AI | GATC | 3 cut(s) | 45, 327, 331 |
| SchI | GAGTC | 1 cut(s) | 265 |
| SetI | ASST | 8 cut(s) | 129, 215, 246, 269, 276, 310, 342, 352 |
| SfaNI | GCATC | 1 cut(s) | 43 |
| Sse9I | AATT | 4 cut(s) | 178, 200, 246, 352 |
| SspMI | CTAG | 2 cut(s) | 77, 287 |
| TaaI | ACNGT | 1 cut(s) | 166 |
| TaiI | ACGT | 1 cut(s) | 276 |
| TaqI | TCGA | 1 cut(s) | 330 |
| TasI | AATT | 4 cut(s) | 178, 200, 246, 352 |
| TatI | WGTACW | 1 cut(s) | 36 |
| TfiI | GAWTC | 2 cut(s) | 104, 185 |
| Tru1I | TTAA | 2 cut(s) | 129, 355 |
| Tru9I | TTAA | 2 cut(s) | 129, 355 |
| TspGWI | ACGGA | 1 cut(s) | 340 |
| XapI | RAATTY | 1 cut(s) | 200 |
| XspI | CTAG | 2 cut(s) | 77, 287 |