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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcyI | GRCGYC | 1 cut(s) | 7 |
| AfaI | GTAC | 1 cut(s) | 280 |
| AfeI | AGCGCT | 1 cut(s) | 48 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 183 |
| AgsI | TTSAA | 3 cut(s) | 97, 104, 133 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 45, 77 |
| AluBI | AGCT | 3 cut(s) | 37, 161, 217 |
| AluI | AGCT | 3 cut(s) | 37, 161, 217 |
| Aor51HI | AGCGCT | 1 cut(s) | 48 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 162 |
| AspLEI | GCGC | 2 cut(s) | 49, 120 |
| BbsI | GAAGAC | 1 cut(s) | 233 |
| BfaI | CTAG | 1 cut(s) | 50 |
| BfmI | CTRYAG | 1 cut(s) | 170 |
| BfoI | RGCGCY | 1 cut(s) | 50 |
| BmsI | GCATC | 1 cut(s) | 16 |
| BpiI | GAAGAC | 1 cut(s) | 233 |
| BsaHI | GRCGYC | 1 cut(s) | 7 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 183 |
| Bse3DI | GCAATG | 1 cut(s) | 73 |
| BseGI | GGATG | 1 cut(s) | 31 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 183 |
| BseMI | GCAATG | 1 cut(s) | 73 |
| BseRI | GAGGAG | 1 cut(s) | 74 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 183 |
| Bsp143I | GATC | 1 cut(s) | 18 |
| BspQI | GCTCTTC | 1 cut(s) | 168 |
| BsrDI | GCAATG | 1 cut(s) | 73 |
| BssMI | GATC | 1 cut(s) | 18 |
| BssNI | GRCGYC | 1 cut(s) | 7 |
| Bst4CI | ACNGT | 2 cut(s) | 174, 278 |
| Bst6I | CTCTTC | 2 cut(s) | 48, 168 |
| BstACI | GRCGYC | 1 cut(s) | 7 |
| BstF5I | GGATG | 1 cut(s) | 31 |
| BstH2I | RGCGCY | 1 cut(s) | 50 |
| BstHHI | GCGC | 2 cut(s) | 49, 120 |
| BstKTI | GATC | 1 cut(s) | 21 |
| BstMBI | GATC | 1 cut(s) | 18 |
| BstSFI | CTRYAG | 1 cut(s) | 170 |
| BstV2I | GAAGAC | 1 cut(s) | 233 |
| BtsCI | GGATG | 1 cut(s) | 31 |
| CfoI | GCGC | 2 cut(s) | 49, 120 |
| CseI | GACGC | 1 cut(s) | 15 |
| Csp6I | GTAC | 1 cut(s) | 279 |
| CviAII | CATG | 1 cut(s) | 178 |
| CviJI | RGCY | 4 cut(s) | 37, 161, 217, 254 |
| CviKI_1 | RGCY | 4 cut(s) | 37, 161, 217, 254 |
| CviQI | GTAC | 1 cut(s) | 279 |
| DpnI | GATC | 1 cut(s) | 20 |
| DpnII | GATC | 1 cut(s) | 18 |
| Eam1104I | CTCTTC | 2 cut(s) | 48, 168 |
| EarI | CTCTTC | 2 cut(s) | 48, 168 |
| Eco47III | AGCGCT | 1 cut(s) | 48 |
| FaeI | CATG | 1 cut(s) | 181 |
| FaiI | YATR | 6 cut(s) | 12, 14, 40, 150, 179, 206 |
| FatI | CATG | 1 cut(s) | 177 |
| FauNDI | CATATG | 1 cut(s) | 12 |
| FokI | GGATG | 1 cut(s) | 38 |
| FspBI | CTAG | 1 cut(s) | 50 |
| GlaI | GCGC | 2 cut(s) | 48, 119 |
| HaeII | RGCGCY | 1 cut(s) | 50 |
| HgaI | GACGC | 1 cut(s) | 15 |
| HhaI | GCGC | 2 cut(s) | 49, 120 |
| Hin1I | GRCGYC | 1 cut(s) | 7 |
| Hin1II | CATG | 1 cut(s) | 181 |
| Hin6I | GCGC | 2 cut(s) | 47, 118 |
| HinP1I | GCGC | 2 cut(s) | 47, 118 |
| HinfI | GANTC | 3 cut(s) | 56, 77, 229 |
| Hpy166II | GTNNAC | 1 cut(s) | 194 |
| Hpy188I | TCNGA | 1 cut(s) | 157 |
| Hpy8I | GTNNAC | 1 cut(s) | 194 |
| Hpy99I | CGWCG | 1 cut(s) | 9 |
| HpyAV | CCTTC | 2 cut(s) | 91, 250 |
| HpyCH4III | ACNGT | 2 cut(s) | 174, 278 |
| HpyCH4V | TGCA | 1 cut(s) | 29 |
| Hsp92I | GRCGYC | 1 cut(s) | 7 |
| Hsp92II | CATG | 1 cut(s) | 181 |
| HspAI | GCGC | 2 cut(s) | 47, 118 |
| Kzo9I | GATC | 1 cut(s) | 18 |
| LguI | GCTCTTC | 1 cut(s) | 168 |
| LpnPI | CCDG | 2 cut(s) | 102, 213 |
| LweI | GCATC | 1 cut(s) | 16 |
| MaeI | CTAG | 1 cut(s) | 50 |
| MalI | GATC | 1 cut(s) | 20 |
| MboI | GATC | 1 cut(s) | 18 |
| MboII | GAAGA | 3 cut(s) | 65, 155, 238 |
| MluCI | AATT | 2 cut(s) | 151, 219 |
| MlyI | GAGTC | 2 cut(s) | 65, 223 |
| MnlI | CCTC | 3 cut(s) | 92, 95, 177 |
| MroXI | GAANNNNTTC | 1 cut(s) | 162 |
| MseI | TTAA | 1 cut(s) | 288 |
| NdeI | CATATG | 1 cut(s) | 12 |
| NdeII | GATC | 1 cut(s) | 18 |
| NlaIII | CATG | 1 cut(s) | 181 |
| PciSI | GCTCTTC | 1 cut(s) | 168 |
| PdmI | GAANNNNTTC | 1 cut(s) | 162 |
| PfeI | GAWTC | 1 cut(s) | 77 |
| PleI | GAGTC | 2 cut(s) | 64, 223 |
| PpsI | GAGTC | 2 cut(s) | 64, 223 |
| RsaI | GTAC | 1 cut(s) | 280 |
| RsaNI | GTAC | 1 cut(s) | 279 |
| SapI | GCTCTTC | 1 cut(s) | 168 |
| SaqAI | TTAA | 1 cut(s) | 288 |
| Sau3AI | GATC | 1 cut(s) | 18 |
| SchI | GAGTC | 2 cut(s) | 65, 223 |
| SetI | ASST | 7 cut(s) | 39, 102, 163, 188, 202, 219, 242 |
| SfaNI | GCATC | 1 cut(s) | 16 |
| SfcI | CTRYAG | 1 cut(s) | 170 |
| Sse9I | AATT | 2 cut(s) | 151, 219 |
| SspMI | CTAG | 1 cut(s) | 50 |
| TaaI | ACNGT | 2 cut(s) | 174, 278 |
| TaqI | TCGA | 1 cut(s) | 17 |
| TasI | AATT | 2 cut(s) | 151, 219 |
| TfiI | GAWTC | 1 cut(s) | 77 |
| Tru1I | TTAA | 1 cut(s) | 288 |
| Tru9I | TTAA | 1 cut(s) | 288 |
| XmnI | GAANNNNTTC | 1 cut(s) | 162 |
| XspI | CTAG | 1 cut(s) | 50 |