Rmu_sc0000071.1_g000001

Amino-transferase class IV

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000071.1
OG0004670
Physical Location & Seq
Forward (+)
1205 .. 6140
4936 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1713 bp
atgacgactactcctctcaggttcttgctcgccaacggcgtcgtctccgaccctgccaacactcctcccgtcaccaccttcctcgagacccacccaggggcttacacgacgactcgcactcacaacaacgcctcgttcgtgttgttttgggataggcacttgaaaaggctcgcggagtccgtgagaatcctgtacaatgcggatcctcagcttctgttcggacccaacaagacttcgcttccattaccgtctcttgcatccaattctcttttgctccacgatctcgtgaatgattcgatgaaaaaagtgctgccaattgctttggaggggaggagtgacggggaggaactgtcgattactactttggtcagtggcaatttggggaaattggagctagaaaaaattggcggggctcttgatgtgagcatttacgttgggatttatgtgccttctgtgtttggtgtcagaggaaacggtgcgcagttggctgtggtgggtcgtgggagagatgttgctgctgctaagtactcggattgggtgaggattaggaagtctttggagaggttgaggccgccctcggggaccgagctcttgttgtccaatgatggggatcggattcttgaaggcagcctgtcaaacttctttgtggtccgccgaaagctgttcgacgaaatgacgactactcctctcaggttcttgctcgccaacggcgtcgtctccgaccctgccaacactcctcccgtcaccaccttcctcgagacccacccaggggcttacacgacgactcgcactcacaacaacgcctcgttcgtgttgttttgggataggcacttgaaaaggctcgcggagtccgtgagaatcctgtacaatgcggatcctcagcttctgttcggacccaacaagacttcgcttccattaccgtctcttgcatccaattctcttttgctccacgatctcgtgaatgattcgatgaaaaaagtgctgccaattgctttggaggggaggagtgacggggaggaactgtcgattactactttggtcagtggcaatttggggaaattggagctagaaaaaattggcggggctcttgatgtgagcatttacgttgggatttatgtgccttctgtgtttggtgtcagaggaaacggtgcgcagttggctgtggtgggtcgtgggagagatgttgctgctgctaagtactcggattgggtgaggattaggaagtctttggagaggttgaggccgccctcggggaccgagctcttgttgtccaatgatggggatcggattcttgaaggcagcctgtcaaacttctttgtggtccgccgaaaggatgacaatgaagctaaagagcaaactatccactgttttgaagtacagacagctcctattagcgagggtgtcctaccaggaattatacgccaactagtcattgaagtatgcttgagcaaaggaatcccttttcgagaagttgcaccactgtggtcagagcatgaattttgggaagaggcgttcattacaaatagcttaagacttatgcagcatgcggagattgtttgtgctcctagttcatgggaatcactgcatggtaattcatggaaggacatatcttggaaagagaagcagtttgaggagggtcctggaatgattactacaatactccagaaggagatcatggaaaaagcagtttcagaaggatactctttgagttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_4

Protein Analysis

570

Amino Acids

62.83

Weight (kDa)

6.23

Isoelectric Point (pI)

31.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 420 - 514 2.8e-10 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 480, 1152
AccII CGCG 2 cut(s) 173, 845
AclWI GGATC 6 cut(s) 197, 210, 618, 869, 882, 1290
AcsI RAATTY 1 cut(s) 1496
AcyI GRCGYC 2 cut(s) 39, 711
AfaI GTAC 5 cut(s) 194, 527, 866, 1199, 1377
AfiI CCNNNNNNNGG 9 cut(s) 96, 97, 578, 606, 768, 769, 1250, 1278, 1330
AflII CTTAAG 1 cut(s) 1528
AgsI TTSAA 6 cut(s) 163, 623, 835, 1295, 1373, 1436
AhlI ACTAGT 1 cut(s) 1426
AjnI CCWGG 4 cut(s) 94, 766, 1408, 1639
AleI CACNNNNGTG 1 cut(s) 1480
AloI GAACNNNNNNTCC 2 cut(s) 1496, 1528
Alw21I GWGCWC 3 cut(s) 591, 1263, 1564
Alw26I GTCTC 6 cut(s) 49, 80, 255, 721, 752, 927
AlwI GGATC 6 cut(s) 197, 210, 618, 869, 882, 1290
AlwNI CAGNNNCTG 2 cut(s) 214, 886
Ama87I CYCGRG 4 cut(s) 83, 577, 755, 1249
AoxI GGCC 2 cut(s) 569, 1241
ApeKI GCWGC 9 cut(s) 310, 515, 518, 627, 982, 1187, 1190, 1299, 1540
ApoI RAATTY 1 cut(s) 1496
AspLEI GCGC 2 cut(s) 481, 1153
AspS9I GGNCC 7 cut(s) 221, 582, 649, 893, 1254, 1321, 1637
AsuHPI GGTGA 4 cut(s) 64, 550, 736, 1222
AvaI CYCGRG 4 cut(s) 83, 577, 755, 1249
AvaII GGWCC 7 cut(s) 221, 582, 649, 893, 1254, 1321, 1637
BamHI GGATCC 2 cut(s) 202, 874
BanII GRGCYC 4 cut(s) 415, 591, 1087, 1263
BauI CACGAG 2 cut(s) 284, 956
Bbv12I GWGCWC 3 cut(s) 591, 1263, 1564
BbvCI CCTCAGC 2 cut(s) 207, 879
BbvI GCAGC 9 cut(s) 297, 502, 505, 639, 969, 1174, 1177, 1311, 1552
BccI CCATC 2 cut(s) 599, 1271
BceAI ACGGC 2 cut(s) 52, 724
BciT130I CCWGG 4 cut(s) 96, 768, 1410, 1641
BciVI GTATCC 1 cut(s) 1691
BcoDI GTCTC 6 cut(s) 49, 80, 255, 721, 752, 927
BcuI ACTAGT 1 cut(s) 1426
BfaI CTAG 4 cut(s) 395, 1067, 1427, 1566
BfrI CTTAAG 1 cut(s) 1528
BfuI GTATCC 1 cut(s) 1691
BmcAI AGTACT 2 cut(s) 527, 1199
Bme1390I CCNGG 4 cut(s) 96, 768, 1410, 1641
Bme18I GGWCC 7 cut(s) 221, 582, 649, 893, 1254, 1321, 1637
BmeT110I CYCGRG 4 cut(s) 83, 577, 755, 1249
BmgT120I GGNCC 7 cut(s) 221, 582, 649, 893, 1254, 1321, 1637
BmiI GGNNCC 7 cut(s) 204, 223, 583, 876, 895, 1255, 1638
BmrFI CCNGG 4 cut(s) 96, 768, 1410, 1641
BmsI GCATC 2 cut(s) 266, 938
BpmI CTGGAG 1 cut(s) 1646
Bpu10I CCTNAGC 2 cut(s) 207, 879
BpuEI CTTGAG 1 cut(s) 1465
BsaBI GATNNNNATC 2 cut(s) 609, 1281
BsaHI GRCGYC 2 cut(s) 39, 711
BsaI GGTCTC 2 cut(s) 80, 752
BsaJI CCNNGG 6 cut(s) 94, 95, 576, 766, 767, 1248
BsaXI ACNNNNNCTCC 7 cut(s) 25, 335, 365, 667, 697, 1007, 1037
Bsc4I CCNNNNNNNGG 9 cut(s) 96, 97, 578, 606, 768, 769, 1250, 1278, 1330
Bse8I GATNNNNATC 2 cut(s) 609, 1281
BseBI CCWGG 4 cut(s) 96, 768, 1410, 1641
BseDI CCNNGG 6 cut(s) 94, 95, 576, 766, 767, 1248
BseGI GGATG 3 cut(s) 257, 929, 1339
BseJI GATNNNNATC 2 cut(s) 609, 1281
BseLI CCNNNNNNNGG 9 cut(s) 96, 97, 578, 606, 768, 769, 1250, 1278, 1330
BseMII CTCAG 4 cut(s) 31, 221, 703, 893
BseRI GAGGAG 6 cut(s) 54, 346, 675, 726, 1018, 1646
BseXI GCAGC 9 cut(s) 297, 502, 505, 639, 969, 1174, 1177, 1311, 1552
Bsh1236I CGCG 2 cut(s) 173, 845
BshFI GGCC 2 cut(s) 571, 1243
BsiHKAI GWGCWC 3 cut(s) 591, 1263, 1564
BsiHKCI CYCGRG 4 cut(s) 83, 577, 755, 1249
BslFI GGGAC 2 cut(s) 595, 1267
BslI CCNNNNNNNGG 9 cut(s) 96, 97, 578, 606, 768, 769, 1250, 1278, 1330
BsmAI GTCTC 6 cut(s) 49, 80, 255, 721, 752, 927
BsmBI CGTCTC 4 cut(s) 49, 255, 721, 927
BsmFI GGGAC 2 cut(s) 595, 1267
BsnI GGCC 2 cut(s) 571, 1243
Bso31I GGTCTC 2 cut(s) 80, 752
BsoBI CYCGRG 4 cut(s) 83, 577, 755, 1249
Bsp1286I GDGCHC 5 cut(s) 415, 591, 1087, 1263, 1564
Bsp1407I TGTACA 2 cut(s) 192, 864
Bsp143I GATC 7 cut(s) 202, 280, 610, 874, 952, 1282, 1671
BspANI GGCC 2 cut(s) 571, 1243
BspCNI CTCAG 4 cut(s) 30, 220, 702, 892
BspFNI CGCG 2 cut(s) 173, 845
BspLI GGNNCC 7 cut(s) 204, 223, 583, 876, 895, 1255, 1638
BspPI GGATC 6 cut(s) 197, 210, 618, 869, 882, 1290
BspTI CTTAAG 1 cut(s) 1528
BspTNI GGTCTC 2 cut(s) 80, 752
BsrGI TGTACA 2 cut(s) 192, 864
BssECI CCNNGG 6 cut(s) 94, 95, 576, 766, 767, 1248
BssMI GATC 7 cut(s) 202, 280, 610, 874, 952, 1282, 1671
BssNI GRCGYC 2 cut(s) 39, 711
BssSI CACGAG 2 cut(s) 284, 956
Bst2BI CACGAG 2 cut(s) 284, 956
Bst2UI CCWGG 4 cut(s) 96, 768, 1410, 1641
Bst4CI ACNGT 8 cut(s) 249, 351, 476, 921, 1023, 1148, 1367, 1482
Bst6I CTCTTC 1 cut(s) 1500
BstACI GRCGYC 2 cut(s) 39, 711
BstAFI CTTAAG 1 cut(s) 1528
BstAUI TGTACA 2 cut(s) 192, 864
BstC8I GCNNGC 5 cut(s) 30, 171, 702, 843, 1545
BstDEI CTNAG 6 cut(s) 17, 207, 522, 689, 879, 1194
BstF5I GGATG 3 cut(s) 257, 929, 1339
BstFNI CGCG 2 cut(s) 173, 845
BstHHI GCGC 2 cut(s) 481, 1153
BstKTI GATC 7 cut(s) 205, 283, 613, 877, 955, 1285, 1674
BstMAI GTCTC 6 cut(s) 49, 80, 255, 721, 752, 927
BstMBI GATC 7 cut(s) 202, 280, 610, 874, 952, 1282, 1671
BstMWI GCNNNNNNNGC 2 cut(s) 485, 1157
BstNI CCWGG 4 cut(s) 96, 768, 1410, 1641
BstNSI RCATGY 1 cut(s) 1547
BstSCI CCNGG 4 cut(s) 94, 766, 1408, 1639
BstUI CGCG 2 cut(s) 173, 845
BstV1I GCAGC 9 cut(s) 297, 502, 505, 639, 969, 1174, 1177, 1311, 1552
BstX2I RGATCY 2 cut(s) 202, 874
BstYI RGATCY 2 cut(s) 202, 874
BsuI GTATCC 1 cut(s) 1691
BsuRI GGCC 2 cut(s) 571, 1243
BtsCI GGATG 3 cut(s) 257, 929, 1339
BtsI GCAGTG 1 cut(s) 1580
BtsIMutI CAGTG 5 cut(s) 376, 1048, 1363, 1478, 1580
Cac8I GCNNGC 5 cut(s) 30, 171, 702, 843, 1545
CaiI CAGNNNCTG 2 cut(s) 214, 886
CfoI GCGC 2 cut(s) 481, 1153
Cfr13I GGNCC 7 cut(s) 221, 582, 649, 893, 1254, 1321, 1637
CseI GACGC 2 cut(s) 28, 700
Csp6I GTAC 5 cut(s) 193, 526, 865, 1198, 1376
CviAII CATG 6 cut(s) 1493, 1544, 1572, 1586, 1596, 1675
CviQI GTAC 5 cut(s) 193, 526, 865, 1198, 1376
DdeI CTNAG 6 cut(s) 17, 207, 522, 689, 879, 1194
DpnI GATC 7 cut(s) 204, 282, 612, 876, 954, 1284, 1673
DpnII GATC 7 cut(s) 202, 280, 610, 874, 952, 1282, 1671
Eam1104I CTCTTC 1 cut(s) 1500
EarI CTCTTC 1 cut(s) 1500
EciI GGCGGA 2 cut(s) 641, 1313
Ecl136II GAGCTC 2 cut(s) 589, 1261
Eco24I GRGCYC 4 cut(s) 415, 591, 1087, 1263
Eco31I GGTCTC 2 cut(s) 80, 752
Eco47I GGWCC 7 cut(s) 221, 582, 649, 893, 1254, 1321, 1637
Eco53kI GAGCTC 2 cut(s) 589, 1261
Eco88I CYCGRG 4 cut(s) 83, 577, 755, 1249
EcoICRI GAGCTC 2 cut(s) 589, 1261
EcoO109I RGGNCCY 1 cut(s) 1637
EcoRII CCWGG 4 cut(s) 94, 766, 1408, 1639
EcoT38I GRGCYC 4 cut(s) 415, 591, 1087, 1263
Esp3I CGTCTC 4 cut(s) 49, 255, 721, 927
FaeI CATG 6 cut(s) 1496, 1547, 1575, 1589, 1599, 1678
FaqI GGGAC 2 cut(s) 595, 1267
FatI CATG 6 cut(s) 1492, 1543, 1571, 1585, 1595, 1674
FauI CCCGC 2 cut(s) 401, 1073
FokI GGATG 3 cut(s) 244, 916, 1346
FriOI GRGCYC 4 cut(s) 415, 591, 1087, 1263
FspBI CTAG 4 cut(s) 395, 1067, 1427, 1566
FspI TGCGCA 2 cut(s) 480, 1152
GlaI GCGC 2 cut(s) 480, 1152
GsuI CTGGAG 1 cut(s) 1646
HaeIII GGCC 2 cut(s) 571, 1243
HgaI GACGC 2 cut(s) 28, 700
HhaI GCGC 2 cut(s) 481, 1153
Hin1I GRCGYC 2 cut(s) 39, 711
Hin1II CATG 6 cut(s) 1496, 1547, 1575, 1589, 1599, 1678
Hin6I GCGC 2 cut(s) 479, 1151
HinP1I GCGC 2 cut(s) 479, 1151
HphI GGTGA 4 cut(s) 64, 550, 736, 1222
Hpy99I CGWCG 5 cut(s) 44, 112, 671, 716, 784
HpyAV CCTTC 9 cut(s) 88, 459, 617, 760, 1131, 1289, 1594, 1660, 1688
HpyCH4III ACNGT 8 cut(s) 249, 351, 476, 921, 1023, 1148, 1367, 1482
HpyCH4IV ACGT 2 cut(s) 432, 1104
HpyCH4V TGCA 5 cut(s) 257, 929, 1475, 1540, 1585
HpyF10VI GCNNNNNNNGC 2 cut(s) 485, 1157
HpyF3I CTNAG 6 cut(s) 17, 207, 522, 689, 879, 1194
HpySE526I ACGT 2 cut(s) 432, 1104
Hsp92I GRCGYC 2 cut(s) 39, 711
Hsp92II CATG 6 cut(s) 1496, 1547, 1575, 1589, 1599, 1678
HspAI GCGC 2 cut(s) 479, 1151
Kzo9I GATC 7 cut(s) 202, 280, 610, 874, 952, 1282, 1671
LmnI GCTCC 6 cut(s) 279, 391, 951, 1063, 1390, 1567
Lsp1109I GCAGC 9 cut(s) 297, 502, 505, 639, 969, 1174, 1177, 1311, 1552
LweI GCATC 2 cut(s) 266, 938
MaeI CTAG 4 cut(s) 395, 1067, 1427, 1566
MaeII ACGT 2 cut(s) 432, 1104
MaeIII GTNAC 4 cut(s) 70, 335, 742, 1007
MalI GATC 7 cut(s) 204, 282, 612, 876, 954, 1284, 1673
MboI GATC 7 cut(s) 202, 280, 610, 874, 952, 1282, 1671
MboII GAAGA 1 cut(s) 1517
MfeI CAATTG 2 cut(s) 315, 987
MflI RGATCY 2 cut(s) 202, 874
MhlI GDGCHC 5 cut(s) 415, 591, 1087, 1263, 1564
MlyI GAGTC 4 cut(s) 106, 185, 778, 857
MmeI TCCRAC 2 cut(s) 72, 744
MseI TTAA 1 cut(s) 1529
MslI CAYNNNNRTG 1 cut(s) 1480
MspCI CTTAAG 1 cut(s) 1528
MspR9I CCNGG 4 cut(s) 96, 768, 1410, 1641
MunI CAATTG 2 cut(s) 315, 987
MvaI CCWGG 4 cut(s) 96, 768, 1410, 1641
MvnI CGCG 2 cut(s) 173, 845
MwoI GCNNNNNNNGC 2 cut(s) 485, 1157
NdeII GATC 7 cut(s) 202, 280, 610, 874, 952, 1282, 1671
NlaIII CATG 6 cut(s) 1496, 1547, 1575, 1589, 1599, 1678
NlaIV GGNNCC 7 cut(s) 204, 223, 583, 876, 895, 1255, 1638
NmuCI GTSAC 4 cut(s) 70, 335, 742, 1007
NsbI TGCGCA 2 cut(s) 480, 1152
NspI RCATGY 1 cut(s) 1547
OliI CACNNNNGTG 1 cut(s) 1480
PaeI GCATGC 1 cut(s) 1547
PaeR7I CTCGAG 2 cut(s) 83, 755
PasI CCCWGGG 2 cut(s) 95, 767
PcsI WCGNNNNNNNCGW 6 cut(s) 36, 135, 177, 708, 807, 849
PfeI GAWTC 8 cut(s) 186, 293, 616, 858, 965, 1288, 1455, 1577
PfoI TCCNGGA 1 cut(s) 1639
PleI GAGTC 4 cut(s) 106, 184, 778, 856
PpsI GAGTC 4 cut(s) 106, 184, 778, 856
PpuMI RGGWCCY 1 cut(s) 1637
Psp124BI GAGCTC 2 cut(s) 591, 1263
Psp5II RGGWCCY 1 cut(s) 1637
Psp6I CCWGG 4 cut(s) 94, 766, 1408, 1639
PspGI CCWGG 4 cut(s) 94, 766, 1408, 1639
PspN4I GGNNCC 7 cut(s) 204, 223, 583, 876, 895, 1255, 1638
PspPI GGNCC 7 cut(s) 221, 582, 649, 893, 1254, 1321, 1637
PspPPI RGGWCCY 1 cut(s) 1637
PstNI CAGNNNCTG 2 cut(s) 214, 886
PsuI RGATCY 2 cut(s) 202, 874
RsaI GTAC 5 cut(s) 194, 527, 866, 1199, 1377
RsaNI GTAC 5 cut(s) 193, 526, 865, 1198, 1376
RseI CAYNNNNRTG 1 cut(s) 1480
SacI GAGCTC 2 cut(s) 591, 1263
SaqAI TTAA 1 cut(s) 1529
Sau3AI GATC 7 cut(s) 202, 280, 610, 874, 952, 1282, 1671
Sau96I GGNCC 7 cut(s) 221, 582, 649, 893, 1254, 1321, 1637
ScaI AGTACT 2 cut(s) 527, 1199
SchI GAGTC 4 cut(s) 106, 185, 778, 857
ScrFI CCNGG 4 cut(s) 96, 768, 1410, 1641
SduI GDGCHC 5 cut(s) 415, 591, 1087, 1263, 1564
SfaNI GCATC 2 cut(s) 266, 938
Sfr274I CTCGAG 2 cut(s) 83, 755
SinI GGWCC 7 cut(s) 221, 582, 649, 893, 1254, 1321, 1637
SlaI CTCGAG 2 cut(s) 83, 755
SmiMI CAYNNNNRTG 1 cut(s) 1480
SmlI CTYRAG 4 cut(s) 83, 755, 1444, 1528
SmoI CTYRAG 4 cut(s) 83, 755, 1444, 1528
SpeI ACTAGT 1 cut(s) 1426
SphI GCATGC 1 cut(s) 1547
SspMI CTAG 4 cut(s) 395, 1067, 1427, 1566
SstI GAGCTC 2 cut(s) 591, 1263
StyD4I CCNGG 4 cut(s) 94, 766, 1408, 1639
TaaI ACNGT 8 cut(s) 249, 351, 476, 921, 1023, 1148, 1367, 1482
TaiI ACGT 2 cut(s) 435, 1107
TaqI TCGA 8 cut(s) 84, 296, 353, 666, 756, 968, 1025, 1465
TaqII GACCGA 2 cut(s) 599, 1271
TatI WGTACW 5 cut(s) 192, 525, 864, 1197, 1375
TauI GCSGC 2 cut(s) 574, 1246
TfiI GAWTC 8 cut(s) 186, 293, 616, 858, 965, 1288, 1455, 1577
Tru1I TTAA 1 cut(s) 1529
Tru9I TTAA 1 cut(s) 1529
TscAI CASTG 5 cut(s) 376, 1048, 1370, 1485, 1587
TseFI GTSAC 4 cut(s) 70, 335, 742, 1007
TseI GCWGC 9 cut(s) 310, 515, 518, 627, 982, 1187, 1190, 1299, 1540
Tsp45I GTSAC 4 cut(s) 70, 335, 742, 1007
TspDTI ATGAA 7 cut(s) 314, 986, 1356, 1504, 1509, 1560, 1584
TspGWI ACGGA 2 cut(s) 169, 841
TspRI CASTG 5 cut(s) 376, 1048, 1370, 1485, 1587
Vha464I CTTAAG 1 cut(s) 1528
VpaK11BI GGWCC 7 cut(s) 221, 582, 649, 893, 1254, 1321, 1637
XapI RAATTY 1 cut(s) 1496
XceI RCATGY 1 cut(s) 1547
XhoI CTCGAG 2 cut(s) 83, 755
XspI CTAG 4 cut(s) 395, 1067, 1427, 1566
ZrmI AGTACT 2 cut(s) 527, 1199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.