Rmu_sc0000294.1_g000021

Protein of unknown function (DUF3054)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000294.1
OG0011949
Physical Location & Seq
Forward (+)
106248 .. 107958
1711 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 819 bp
atgctaattctgagccaagctcccaccggagccttatcggccagctgttctcgcaagccccctctcaaattcaccaacccgaacccgccaatcttctcgacccgaaaacccaaattccttcccggacccaacccgtccaagtccctcaacctcacgctcgctaaagccgtcgacggaggaggaggaggaggcgtcgattccacctcacccgccgccgccgtcaagccgctcccctctcctcctccgtcgccgttagacaatgaccagcccgtcttcgtcgccggcgagaatatgcccttggaaggcgtcattcagttcgacaagcccaggcccagctcctctgctcgaatcagaaaatggggtaaggtggcattgctcgccggcggggatgttctggctttgctgacgttcgctgcaattgggagattcagtcatggcttccctgttttggacttcgaaaccctccgcactgctgacccttttattgccggttggtttttgagtgcttatttcctcggaggctttggggaagacggacgtggaatcaatggtctgcctaaggctgttactgcttctgcaaagtcatgggccctcggaatcccacttgggatagtcataagggcatcaacagcaggtcacattccctcattcagttttgtgttggtatcaatggggagcactgctgttctacttttgggatggaggacgttattgtataaattccttcctggtgataagagcaaaaagagtgatgtatatcgccgtggtagcccgtttgagttgtttgagttgctcacatctctagtacgaagatggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3054

Protein Analysis

272

Amino Acids

28.81

Weight (kDa)

10.13

Isoelectric Point (pI)

41.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3054 PF11255 128 - 237 7.8e-30 Protein of unknown function (DUF3054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011949)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44920
fragaria_vesca FvH4_2g32650
malus_domestica MD08G1077300.v1.1 MD15G1064800.v1.1
prunus_persica Prupe.1G418700_v2.0.a1
pyrus_communis pycom08g06420 pycom15g06150
rosa_chinensis RchiOBHm_Chr6g0310081
rosa_laevigata RLG00000010513
rosa_multiflora Rmu_sc0000294.1_g000021 Rmu_sc0038452.1_g000001
rosa_roxburghii Rroxscaffold_7G00158900
rosa_rugosa Rorug06G0377200 Rorug06G0377200
rosa_samantha Rh6AG491100 Rh6BG500500 Rh6CG505400 Rh6DG491400
rosa_wichuraiana Rw6G042730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 269
Acc36I ACCTGC 1 cut(s) 623
AccBSI CCGCTC 1 cut(s) 229
AccI GTMKAC 1 cut(s) 171
AciI CCGC 7 cut(s) 86, 210, 213, 216, 227, 384, 466
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 3 cut(s) 68, 113, 719
AcyI GRCGYC 2 cut(s) 192, 306
AfaI GTAC 1 cut(s) 807
AfiI CCNNNNNNNGG 2 cut(s) 302, 448
AjiI CACGTC 1 cut(s) 539
AjnI CCWGG 2 cut(s) 326, 727
AjuI GAANNNNNNNTTGG 2 cut(s) 281, 313
AluBI AGCT 3 cut(s) 20, 45, 336
AluI AGCT 3 cut(s) 20, 45, 336
Alw21I GWGCWC 1 cut(s) 680
AoxI GGCC 3 cut(s) 39, 329, 588
ApaI GGGCCC 1 cut(s) 592
ApeKI GCWGC 1 cut(s) 413
ApoI RAATTY 3 cut(s) 68, 113, 719
AspS9I GGNCC 4 cut(s) 125, 330, 588, 589
AsuC2I CCSGG 1 cut(s) 123
AsuHPI GGTGA 3 cut(s) 64, 198, 743
AsuII TTCGAA 1 cut(s) 456
AvaII GGWCC 1 cut(s) 125
AxyI CCTNAGG 1 cut(s) 558
BaeGI GKGCMC 1 cut(s) 592
BanII GRGCYC 1 cut(s) 592
BbsI GAAGAC 2 cut(s) 265, 537
Bbv12I GWGCWC 1 cut(s) 680
BbvI GCAGC 1 cut(s) 400
BccI CCATC 2 cut(s) 693, 807
BceAI ACGGC 4 cut(s) 152, 203, 235, 747
BciT130I CCWGG 2 cut(s) 328, 729
BcnI CCSGG 1 cut(s) 123
BfaI CTAG 1 cut(s) 803
BfuAI ACCTGC 1 cut(s) 623
BglI GCCNNNNNGGC 1 cut(s) 38
BisI GCNGC 4 cut(s) 213, 216, 227, 414
BlsI GCNGC 4 cut(s) 214, 217, 228, 415
Bme1390I CCNGG 3 cut(s) 123, 328, 729
Bme18I GGWCC 1 cut(s) 125
BmgBI CACGTC 1 cut(s) 539
BmgT120I GGNCC 4 cut(s) 125, 330, 588, 589
BmiI GGNNCC 3 cut(s) 31, 127, 590
BmrFI CCNGG 3 cut(s) 123, 328, 729
BmsI GCATC 1 cut(s) 632
BpiI GAAGAC 2 cut(s) 265, 537
BplI GAGNNNNNCTC 1 cut(s) 36
Bpu14I TTCGAA 1 cut(s) 456
BpuMI CCSGG 1 cut(s) 123
BsaBI GATNNNNATC 1 cut(s) 756
BsaHI GRCGYC 2 cut(s) 192, 306
BsaJI CCNNGG 5 cut(s) 297, 326, 514, 592, 763
BsaWI WCCGGW 1 cut(s) 26
BsaXI ACNNNNNCTCC 4 cut(s) 177, 207, 415, 445
Bsc4I CCNNNNNNNGG 2 cut(s) 302, 448
Bse118I RCCGGY 3 cut(s) 281, 380, 488
Bse21I CCTNAGG 1 cut(s) 558
Bse3DI GCAATG 1 cut(s) 371
Bse8I GATNNNNATC 1 cut(s) 756
BseBI CCWGG 2 cut(s) 328, 729
BseDI CCNNGG 5 cut(s) 297, 326, 514, 592, 763
BseGI GGATG 2 cut(s) 394, 704
BseJI GATNNNNATC 1 cut(s) 756
BseLI CCNNNNNNNGG 2 cut(s) 302, 448
BseMI GCAATG 1 cut(s) 371
BseRI GAGGAG 7 cut(s) 192, 195, 198, 201, 228, 231, 328
BseSI GKGCMC 1 cut(s) 592
BseXI GCAGC 1 cut(s) 400
BseYI CCCAGC 1 cut(s) 332
BshFI GGCC 3 cut(s) 41, 331, 590
BsiHKAI GWGCWC 1 cut(s) 680
BsiSI CCGG 5 cut(s) 27, 123, 282, 381, 489
BslFI GGGAC 1 cut(s) 127
BslI CCNNNNNNNGG 2 cut(s) 302, 448
BsmFI GGGAC 1 cut(s) 127
BsnI GGCC 3 cut(s) 41, 331, 590
Bsp119I TTCGAA 1 cut(s) 456
Bsp120I GGGCCC 1 cut(s) 588
Bsp1286I GDGCHC 2 cut(s) 592, 680
BspACI CCGC 7 cut(s) 86, 210, 213, 216, 227, 384, 466
BspANI GGCC 3 cut(s) 41, 331, 590
BspLI GGNNCC 3 cut(s) 31, 127, 590
BspMI ACCTGC 1 cut(s) 623
BspT104I TTCGAA 1 cut(s) 456
BsrBI CCGCTC 1 cut(s) 229
BsrDI GCAATG 1 cut(s) 371
BsrFI RCCGGY 3 cut(s) 281, 380, 488
BssAI RCCGGY 3 cut(s) 281, 380, 488
BssECI CCNNGG 5 cut(s) 297, 326, 514, 592, 763
BssNI GRCGYC 2 cut(s) 192, 306
BssT1I CCWWGG 1 cut(s) 297
Bst2UI CCWGG 2 cut(s) 328, 729
BstACI GRCGYC 2 cut(s) 192, 306
BstBI TTCGAA 1 cut(s) 456
BstC8I GCNNGC 6 cut(s) 43, 56, 159, 283, 378, 382
BstDEI CTNAG 2 cut(s) 11, 558
BstDSI CCRYGG 1 cut(s) 763
BstF5I GGATG 2 cut(s) 394, 704
BstMWI GCNNNNNNNGC 6 cut(s) 38, 51, 377, 569, 629, 768
BstNI CCWGG 2 cut(s) 328, 729
BstSCI CCNGG 3 cut(s) 121, 326, 727
BstSLI GKGCMC 1 cut(s) 592
BstV1I GCAGC 1 cut(s) 400
BstV2I GAAGAC 2 cut(s) 265, 537
Bsu36I CCTNAGG 1 cut(s) 558
BsuRI GGCC 3 cut(s) 41, 331, 590
BtgI CCRYGG 1 cut(s) 763
BtrI CACGTC 1 cut(s) 539
BtsCI GGATG 2 cut(s) 394, 704
BtsI GCAGTG 2 cut(s) 468, 678
BtsIMutI CAGTG 2 cut(s) 468, 678
BveI ACCTGC 1 cut(s) 623
Cac8I GCNNGC 6 cut(s) 43, 56, 159, 283, 378, 382
Cfr10I RCCGGY 3 cut(s) 281, 380, 488
Cfr13I GGNCC 4 cut(s) 125, 330, 588, 589
CseI GACGC 2 cut(s) 181, 295
Csp6I GTAC 1 cut(s) 806
CviAII CATG 2 cut(s) 434, 585
CviQI GTAC 1 cut(s) 806
DdeI CTNAG 2 cut(s) 11, 558
DrdI GACNNNNNNGTC 1 cut(s) 269
DseDI GACNNNNNNGTC 1 cut(s) 269
EaeI YGGCCR 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 297
Eco24I GRGCYC 1 cut(s) 592
Eco47I GGWCC 1 cut(s) 125
Eco81I CCTNAGG 1 cut(s) 558
EcoO109I RGGNCCY 1 cut(s) 589
EcoRII CCWGG 2 cut(s) 326, 727
EcoT14I CCWWGG 1 cut(s) 297
EcoT38I GRGCYC 1 cut(s) 592
ErhI CCWWGG 1 cut(s) 297
FaeI CATG 2 cut(s) 437, 588
FaiI YATR 6 cut(s) 293, 435, 586, 617, 717, 757
FaqI GGGAC 1 cut(s) 127
FatI CATG 2 cut(s) 433, 584
FauI CCCGC 3 cut(s) 93, 217, 377
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 4 cut(s) 213, 216, 227, 414
FokI GGATG 2 cut(s) 401, 711
FriOI GRGCYC 1 cut(s) 592
Fsp4HI GCNGC 4 cut(s) 213, 216, 227, 414
FspBI CTAG 1 cut(s) 803
GluI GCNGC 4 cut(s) 213, 216, 227, 414
GsaI CCCAGC 1 cut(s) 336
HaeIII GGCC 3 cut(s) 41, 331, 590
HapII CCGG 5 cut(s) 27, 123, 282, 381, 489
HgaI GACGC 2 cut(s) 181, 295
Hin1I GRCGYC 2 cut(s) 192, 306
Hin1II CATG 2 cut(s) 437, 588
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 5 cut(s) 197, 348, 426, 543, 597
HpaII CCGG 5 cut(s) 27, 123, 282, 381, 489
HphI GGTGA 3 cut(s) 64, 198, 743
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 4 cut(s) 12, 353, 518, 596
Hpy188III TCNNGA 1 cut(s) 97
Hpy8I GTNNAC 1 cut(s) 172
Hpy99I CGWCG 5 cut(s) 173, 176, 197, 250, 281
HpyAV CCTTC 3 cut(s) 128, 296, 734
HpyCH4IV ACGT 3 cut(s) 407, 538, 707
HpyCH4V TGCA 2 cut(s) 416, 578
HpyF10VI GCNNNNNNNGC 6 cut(s) 38, 51, 377, 569, 629, 768
HpyF3I CTNAG 2 cut(s) 11, 558
HpySE526I ACGT 3 cut(s) 407, 538, 707
Hsp92I GRCGYC 2 cut(s) 192, 306
Hsp92II CATG 2 cut(s) 437, 588
KroI GCCGGC 2 cut(s) 281, 380
KroNI GCCGGC 2 cut(s) 283, 382
LmnI GCTCC 5 cut(s) 25, 29, 234, 341, 675
Lsp1109I GCAGC 1 cut(s) 400
LweI GCATC 1 cut(s) 632
MaeI CTAG 1 cut(s) 803
MaeII ACGT 3 cut(s) 407, 538, 707
MaeIII GTNAC 2 cut(s) 565, 635
MbiI CCGCTC 1 cut(s) 229
MboII GAAGA 3 cut(s) 85, 265, 542
MfeI CAATTG 1 cut(s) 417
MhlI GDGCHC 2 cut(s) 592, 680
MluCI AATT 5 cut(s) 6, 68, 113, 417, 719
MreI CGCCGGCG 2 cut(s) 281, 380
MroNI GCCGGC 2 cut(s) 281, 380
MspA1I CMGCKG 1 cut(s) 45
MspI CCGG 5 cut(s) 27, 123, 282, 381, 489
MspR9I CCNGG 3 cut(s) 123, 328, 729
MunI CAATTG 1 cut(s) 417
MvaI CCWGG 2 cut(s) 328, 729
MwoI GCNNNNNNNGC 6 cut(s) 38, 51, 377, 569, 629, 768
NaeI GCCGGC 2 cut(s) 283, 382
NciI CCSGG 1 cut(s) 123
NgoMIV GCCGGC 2 cut(s) 281, 380
NlaIII CATG 2 cut(s) 437, 588
NlaIV GGNNCC 3 cut(s) 31, 127, 590
NmuCI GTSAC 1 cut(s) 635
NspV TTCGAA 1 cut(s) 456
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 282
PdiI GCCGGC 2 cut(s) 283, 382
PfeI GAWTC 5 cut(s) 197, 348, 426, 543, 597
PfoI TCCNGGA 1 cut(s) 121
PkrI GCNGC 4 cut(s) 214, 217, 228, 415
Psp6I CCWGG 2 cut(s) 326, 727
PspFI CCCAGC 1 cut(s) 332
PspGI CCWGG 2 cut(s) 326, 727
PspN4I GGNNCC 3 cut(s) 31, 127, 590
PspOMI GGGCCC 1 cut(s) 588
PspPI GGNCC 4 cut(s) 125, 330, 588, 589
PvuII CAGCTG 1 cut(s) 45
RsaI GTAC 1 cut(s) 807
RsaNI GTAC 1 cut(s) 806
SalI GTCGAC 1 cut(s) 170
SatI GCNGC 4 cut(s) 213, 216, 227, 414
Sau96I GGNCC 4 cut(s) 125, 330, 588, 589
ScrFI CCNGG 3 cut(s) 123, 328, 729
SduI GDGCHC 2 cut(s) 592, 680
SfaNI GCATC 1 cut(s) 632
SfuI TTCGAA 1 cut(s) 456
SgrAI CRCCGGYG 2 cut(s) 281, 380
SgrDI CGTCGACG 1 cut(s) 170
SinI GGWCC 1 cut(s) 125
Sse9I AATT 5 cut(s) 6, 68, 113, 417, 719
SsiI CCGC 7 cut(s) 86, 210, 213, 216, 227, 384, 466
SspMI CTAG 1 cut(s) 803
StyD4I CCNGG 3 cut(s) 121, 326, 727
StyI CCWWGG 1 cut(s) 297
TaiI ACGT 3 cut(s) 410, 541, 710
TaqI TCGA 6 cut(s) 98, 171, 195, 318, 346, 456
TasI AATT 5 cut(s) 6, 68, 113, 417, 719
TauI GCSGC 3 cut(s) 215, 218, 229
TfiI GAWTC 5 cut(s) 197, 348, 426, 543, 597
TscAI CASTG 2 cut(s) 475, 685
TseFI GTSAC 1 cut(s) 635
TseI GCWGC 1 cut(s) 413
Tsp45I GTSAC 1 cut(s) 635
TspGWI ACGGA 3 cut(s) 189, 234, 549
TspRI CASTG 2 cut(s) 475, 685
VpaK11BI GGWCC 1 cut(s) 125
XapI RAATTY 3 cut(s) 68, 113, 719
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 1 cut(s) 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.