Rmu_sc0000368.1_g000041

Domain of unknown function (DUF4535)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000368.1
OG0007078
Physical Location & Seq
Reverse (-)
182431 .. 184926
2496 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 471 bp
atgggaacctattggtgttttctgtttgccaaagatgaaactcatggcggtggattggaagaactcaagaagtcaccggcgatggcgaatcggcgtcactcagctcatggcgtcgctactcgaggagttgtggctatactcgacggtgccgatatagaggaagctcgaaggcgccgctctggaattgttggcgaaccagtgcgagctgctccctccgccattaacgagtgtctgagtctccgattggtgcttgactcgtttgggtttcacaaagttgagattgaagatggttgccaagttgtgggttttgattactttgaagaaattttggtcggaatctacgtctctcagaacgataacgtccccaacatcgataagtttgccaagaccttcctcctaatcggcaacctcgaagagacttaccggaagcccaagaaaagggagagagacgagatcgagatcaagatctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4535

Protein Analysis

156

Amino Acids

17.51

Weight (kDa)

5.85

Isoelectric Point (pI)

68.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4535 PF15054 110 - 144 1.5e-08 Domain of unknown function (DUF4535)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 146, 171
AccB7I CCANNNNNTGG 1 cut(s) 301
AccBSI CCGCTC 1 cut(s) 177
AciI CCGC 3 cut(s) 48, 175, 216
AcsI RAATTY 1 cut(s) 324
AcyI GRCGYC 3 cut(s) 94, 111, 172
AfiI CCNNNNNNNGG 2 cut(s) 301, 438
AgsI TTSAA 2 cut(s) 284, 320
AluBI AGCT 3 cut(s) 104, 164, 206
AluI AGCT 3 cut(s) 104, 164, 206
Alw26I GTCTC 4 cut(s) 242, 349, 410, 441
Ama87I CYCGRG 1 cut(s) 120
ApeKI GCWGC 1 cut(s) 206
ApoI RAATTY 1 cut(s) 324
AspLEI GCGC 1 cut(s) 174
AsuHPI GGTGA 1 cut(s) 66
AvaI CYCGRG 1 cut(s) 120
BanI GGYRCC 2 cut(s) 146, 171
BbvI GCAGC 1 cut(s) 193
BccI CCATC 2 cut(s) 76, 281
BcgI CGANNNNNNTGC 2 cut(s) 362, 396
BcoDI GTCTC 4 cut(s) 242, 349, 410, 441
BfaI CTAG 1 cut(s) 469
BfoI RGCGCY 1 cut(s) 175
BglII AGATCT 1 cut(s) 465
BisI GCNGC 2 cut(s) 175, 207
BlsI GCNGC 2 cut(s) 176, 208
BmeT110I CYCGRG 1 cut(s) 120
BmiI GGNNCC 3 cut(s) 7, 148, 173
BpuEI CTTGAG 1 cut(s) 50
Bsa29I ATCGAT 1 cut(s) 372
BsaBI GATNNNNATC 2 cut(s) 458, 464
BsaHI GRCGYC 3 cut(s) 94, 111, 172
BsaWI WCCGGW 1 cut(s) 423
Bsc4I CCNNNNNNNGG 2 cut(s) 301, 438
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 1 cut(s) 197
Bse8I GATNNNNATC 2 cut(s) 458, 464
BseCI ATCGAT 1 cut(s) 372
BseJI GATNNNNATC 2 cut(s) 458, 464
BseLI CCNNNNNNNGG 2 cut(s) 301, 438
BseMII CTCAG 3 cut(s) 114, 224, 362
BseNI ACTGG 1 cut(s) 197
BseRI GAGGAG 1 cut(s) 138
BseXI GCAGC 1 cut(s) 193
BshNI GGYRCC 2 cut(s) 146, 171
BshVI ATCGAT 1 cut(s) 372
BsiHKCI CYCGRG 1 cut(s) 120
BsiSI CCGG 2 cut(s) 77, 424
BslFI GGGAC 1 cut(s) 347
BslI CCNNNNNNNGG 2 cut(s) 301, 438
BsmAI GTCTC 4 cut(s) 242, 349, 410, 441
BsmBI CGTCTC 2 cut(s) 349, 441
BsmFI GGGAC 1 cut(s) 347
BsoBI CYCGRG 1 cut(s) 120
Bsp143I GATC 3 cut(s) 453, 459, 465
BspACI CCGC 3 cut(s) 48, 175, 216
BspCNI CTCAG 3 cut(s) 113, 225, 361
BspDI ATCGAT 1 cut(s) 372
BspLI GGNNCC 3 cut(s) 7, 148, 173
BspT107I GGYRCC 2 cut(s) 146, 171
BsrBI CCGCTC 1 cut(s) 177
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 1 cut(s) 197
BssAI RCCGGY 1 cut(s) 76
BssMI GATC 3 cut(s) 453, 459, 465
BssNI GRCGYC 3 cut(s) 94, 111, 172
Bst4CI ACNGT 1 cut(s) 146
Bst6I CTCTTC 1 cut(s) 408
BstACI GRCGYC 3 cut(s) 94, 111, 172
BstC8I GCNNGC 1 cut(s) 204
BstDEI CTNAG 3 cut(s) 100, 233, 348
BstH2I RGCGCY 1 cut(s) 175
BstHHI GCGC 1 cut(s) 174
BstKTI GATC 3 cut(s) 456, 462, 468
BstMAI GTCTC 4 cut(s) 242, 349, 410, 441
BstMBI GATC 3 cut(s) 453, 459, 465
BstMWI GCNNNNNNNGC 1 cut(s) 215
BstV1I GCAGC 1 cut(s) 193
BstX2I RGATCY 1 cut(s) 465
BstYI RGATCY 1 cut(s) 465
Bsu15I ATCGAT 1 cut(s) 372
BsuTUI ATCGAT 1 cut(s) 372
BtgZI GCGATG 1 cut(s) 95
BtsIMutI CAGTG 1 cut(s) 204
Cac8I GCNNGC 1 cut(s) 204
CfoI GCGC 1 cut(s) 174
Cfr10I RCCGGY 1 cut(s) 76
ClaI ATCGAT 1 cut(s) 372
CseI GACGC 2 cut(s) 83, 100
CviAII CATG 2 cut(s) 44, 107
CviJI RGCY 5 cut(s) 104, 134, 164, 206, 430
CviKI_1 RGCY 5 cut(s) 104, 134, 164, 206, 430
DdeI CTNAG 3 cut(s) 100, 233, 348
DinI GGCGCC 1 cut(s) 173
DpnI GATC 3 cut(s) 455, 461, 467
DpnII GATC 3 cut(s) 453, 459, 465
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
EciI GGCGGA 1 cut(s) 205
Eco88I CYCGRG 1 cut(s) 120
EgeI GGCGCC 1 cut(s) 173
EheI GGCGCC 1 cut(s) 173
Esp3I CGTCTC 2 cut(s) 349, 441
FaeI CATG 2 cut(s) 47, 110
FaiI YATR 4 cut(s) 45, 108, 137, 155
FaqI GGGAC 1 cut(s) 347
FatI CATG 2 cut(s) 43, 106
Fnu4HI GCNGC 2 cut(s) 175, 207
Fsp4HI GCNGC 2 cut(s) 175, 207
FspBI CTAG 1 cut(s) 469
GlaI GCGC 1 cut(s) 173
GluI GCNGC 2 cut(s) 175, 207
HaeII RGCGCY 1 cut(s) 175
HapII CCGG 2 cut(s) 77, 424
HgaI GACGC 2 cut(s) 83, 100
HhaI GCGC 1 cut(s) 174
Hin1I GRCGYC 3 cut(s) 94, 111, 172
Hin1II CATG 2 cut(s) 47, 110
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HinfI GANTC 4 cut(s) 88, 235, 254, 336
HpaII CCGG 2 cut(s) 77, 424
HphI GGTGA 1 cut(s) 66
Hpy188I TCNGA 4 cut(s) 234, 242, 335, 351
Hpy188III TCNNGA 4 cut(s) 67, 180, 457, 463
Hpy99I CGWCG 2 cut(s) 116, 146
HpyAV CCTTC 2 cut(s) 162, 400
HpyCH4III ACNGT 1 cut(s) 146
HpyCH4IV ACGT 2 cut(s) 342, 360
HpyF10VI GCNNNNNNNGC 1 cut(s) 215
HpyF3I CTNAG 3 cut(s) 100, 233, 348
HpySE526I ACGT 2 cut(s) 342, 360
Hsp92I GRCGYC 3 cut(s) 94, 111, 172
Hsp92II CATG 2 cut(s) 47, 110
HspAI GCGC 1 cut(s) 172
KasI GGCGCC 1 cut(s) 171
Kzo9I GATC 3 cut(s) 453, 459, 465
LmnI GCTCC 1 cut(s) 214
LpnPI CCDG 4 cut(s) 90, 165, 210, 437
Lsp1109I GCAGC 1 cut(s) 193
MaeI CTAG 1 cut(s) 469
MaeII ACGT 2 cut(s) 342, 360
MaeIII GTNAC 2 cut(s) 72, 95
MalI GATC 3 cut(s) 455, 461, 467
MbiI CCGCTC 1 cut(s) 177
MboI GATC 3 cut(s) 453, 459, 465
MboII GAAGA 4 cut(s) 71, 296, 332, 425
MflI RGATCY 1 cut(s) 465
MluCI AATT 2 cut(s) 183, 324
Mly113I GGCGCC 1 cut(s) 172
MlyI GAGTC 2 cut(s) 244, 248
MmeI TCCRAC 1 cut(s) 313
MnlI CCTC 5 cut(s) 116, 151, 223, 404, 419
MseI TTAA 1 cut(s) 222
MslI CAYNNNNRTG 1 cut(s) 48
MspI CCGG 2 cut(s) 77, 424
MwoI GCNNNNNNNGC 1 cut(s) 215
NarI GGCGCC 1 cut(s) 172
NdeII GATC 3 cut(s) 453, 459, 465
NlaIII CATG 2 cut(s) 47, 110
NlaIV GGNNCC 3 cut(s) 7, 148, 173
NmuCI GTSAC 2 cut(s) 72, 95
PaeR7I CTCGAG 1 cut(s) 120
PcsI WCGNNNNNNNCGW 3 cut(s) 147, 339, 408
PfeI GAWTC 2 cut(s) 88, 336
PflMI CCANNNNNTGG 1 cut(s) 301
PkrI GCNGC 2 cut(s) 176, 208
PleI GAGTC 2 cut(s) 243, 248
PluTI GGCGCC 1 cut(s) 175
PpsI GAGTC 2 cut(s) 243, 248
PspN4I GGNNCC 3 cut(s) 7, 148, 173
PspXI VCTCGAGB 1 cut(s) 120
PsuI RGATCY 1 cut(s) 465
RseI CAYNNNNRTG 1 cut(s) 48
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 2 cut(s) 175, 207
Sau3AI GATC 3 cut(s) 453, 459, 465
SchI GAGTC 2 cut(s) 244, 248
SetI ASST 8 cut(s) 11, 106, 166, 208, 345, 363, 392, 411
SfoI GGCGCC 1 cut(s) 173
Sfr274I CTCGAG 1 cut(s) 120
SgrAI CRCCGGYG 1 cut(s) 76
SlaI CTCGAG 1 cut(s) 120
SmiMI CAYNNNNRTG 1 cut(s) 48
SmlI CTYRAG 2 cut(s) 65, 120
SmoI CTYRAG 2 cut(s) 65, 120
Sse9I AATT 2 cut(s) 183, 324
SsiI CCGC 3 cut(s) 48, 175, 216
SspDI GGCGCC 1 cut(s) 171
SspMI CTAG 1 cut(s) 469
TaaI ACNGT 1 cut(s) 146
TaiI ACGT 2 cut(s) 345, 363
TaqI TCGA 6 cut(s) 121, 141, 166, 372, 411, 456
TasI AATT 2 cut(s) 183, 324
TauI GCSGC 1 cut(s) 177
TfiI GAWTC 2 cut(s) 88, 336
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 1 cut(s) 204
TseFI GTSAC 2 cut(s) 72, 95
TseI GCWGC 1 cut(s) 206
Tsp45I GTSAC 2 cut(s) 72, 95
TspDTI ATGAA 1 cut(s) 51
TspRI CASTG 1 cut(s) 204
Van91I CCANNNNNTGG 1 cut(s) 301
XapI RAATTY 1 cut(s) 324
XhoI CTCGAG 1 cut(s) 120
XspI CTAG 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.