Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0000435.1

Orthogroup ID

OG0010845

Physical Location & Seq

Genomic Coordinates Reverse (-)

171675 .. 182094

Gene Structure 10420 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3876 bp

atggagtttttaggtgtcggaattcggaaaagctatggaggtttgtgggctaaactggcaattttactgtttctgtgtactattgaacgagcggtgtgcgattccgactatttgattgggctcgggagctatgacatcaccggaccggcggctgatgtcaatatgatggggtatgctaacacggagcagatcgctgccggagttcatttcaggctgcgagctcgctcgttcattgtggcggagccgaagggcaaccgagtggtgtttgtgaatcttgatgcttgcatggcctcgcagcttgtgaaattgaaagtgattgagaggttgaaggcaaggtatggggatctatacactgagaagaatgtagctattagtggaattcacacccatgctggccctgggggttatctacagtatattgtgtatattgtaacatctctcgggtttgtgcgtcagtcatttgatgcccttgttgatggcattgagcaaagcgttattcaagcccatgaaaatcttgcgccaggatcagtttttgttaataagggagaaatcttagatgctggagtaaaccgtagtcctagtgcttatctcaataatcctgcagcagagcgcagtaaatacaaatatgatgtcgataaagaaatgactcttctgaagtttgtcgataatcaatggggtccagtgggtagcttcaattggtttgcaactcatggaacttctatgagtcggacaaactcattaattagtggggataacaagggtgctgctgcacgatttatggaagactggtttgaggagaatggtggtagaagtgcatattctgatgatattgatgctgccgatataccgcgaagagtgtcaaacgtagtttctgaccatcatgataatcaccatgagttactggagcttgctgcatcctttcagtctcctcctggtaagccagcaaccaggtccttgagtgttgcaagacgtgtcaggggtgtactaaggcaggctaacaagcctagatttgtttctgccttttgtcaatcaaactgtggtgatgtgagcccaaatgttctaggagctttctgcacagatactgggctaccttgtgatttcaatcacagcacctgtggtgggaagaatgagttgtgctatggccgaggaccgggttacccagatgaatttgaaagtacacgtattattgctgagaggcaactgagaaaagctgtggatcttttcaacaaagcatctgaacagttgaccgggaagattgaatatcgccacacttacctcaacttctcccagcttgaagtaacccttcccaagaaggggggaggttctgaggtggtaaagacatgtcctgctgcaatgggatttggatttgctgctggaaccactgatggacctggagcttttgatttcaagcaaggggatgataagggaaatcctttctggagattagtgcgcaatgtactcaaaacaccaggccaggaacaaattgattgtcagagtccaaagccaatcttgcttgatactggtgaaatgaagcaaccatatgactgggcgccttcaatacttccaattcagatcttccggatagggcagcttgtcattctcagtgtacctggagaatttacaacaatggctggtaggcggctccgtgatgctgtgaaaaccgtactgactagtggtggtcatggtggaaatattcacgttgtgctagcaggattgactaatacttattctcagtatattacgacctttgaagagtacgaagtgcagagatatgagggtgcatccactctctatggaccacacacgctaagtgcctacattcaggagttcaagaagcttgctacagctctcatcagtaaccaacctgtcgcatcaggtccacaacccccagatctccttgataagcagataagcttgcttactccagttgttatggatggaacccctgttggtgtcagttttggggattgcagttcggatgtgcctcaaaactccacctttaagagaggtcatgacatggtaacagttaccttctggtcggcttgtcctcggaatgacctattgacggaaggtacatttgcccttgtggagattctccatggaaaggatacttgggttccagcttacgatgacgatgatttctgtctgcgctttaagtggtcaagaccttccaaactcagtactagaagtcaggcgaccatggaatggagaattcctcagtccgcaactcccggcgtatacagaataaggcattttggtgcctctaagagcctggttggatcgattcgccactttacggctgagctccgactgcttcagactttccgacacggagcgaagaatattcttaaaagaatattcgagaagccaggcaggcttgagttaggagagttcaaactgaaaacgaagaagaagaagaaagaaagaaagaagaaaatgtcggtccaagagtatctggataatcacttgctctctcgcaaaatcgaagacgccgtcaatgccgccgtcagggccaaagccgccgatcccgtcctcttcatctcgaatcatatgaggaaggcgattccctcagtgataacgaaggtcaaagctcggcagatcctcgatagcagaggaattcctactgttgaagttgatcttcatactaacaagggcatgtttcgtgcttcagctccgagttccgatcttactggatttggtgaaactaggtatgaggccgttgagttgcgagatggggataagggatcgtatctcggaagtagtgtgaataaagctgttaagaatgtgaatgagaagatatgtgaagcattggttgggatggatcctacgcttcaatcgcagattgatcaggcaatgatagacttggataagacagaaaagaagggtgaacttggagtgaatgccatattggctgtgtcgatggctgcttgcaaagctggggctgctcagaaagaggttccactgtacaaacacattgctgacctttctggaagaaacaccctgaccctgcctgttcctgctttcactcttataagtggaggaaagcgtgctgggaatagtctagccattcaggaaattatgattctcccaattggagctaacaggtttgcggaggcactacaaatgggatcagaaacttatcatcacttgaaggctgttattgtagacaagtatggcgcacatggatgtaatgtcggtgaagatggtggttttgctcccaacatctccagttttagagaaggcttggatcttgtaaaagaggctatcagcagaacaggctataatgaccggataaaaatagcaattgatgttgctgctacgaacttctgcatgggtacaaagtatgacctggactacaaatcaccgaataagtcagggcaaaactttaagtcggcagaagatatgattaagttgtatagagaactttgtactgagtacccaattgtatcaattgaagatccgtttgataaggaggcctgggaacacaccaaacggttttctaatcttgcaatttgtcaggtagttggagatgacttgttaatgtcaaattcacaacgtatgaagaaagcaatagaggaatctacgtgtgatgctcttcttctgaagattaaccagattgggacagtgactgaagccattgaagtcgttaaactggcaaaggatgcccactggggagttgtggcatctcatagatgtggtgaaactgaagagtcctttatagctgacttctctgttggtctctctactggtcaaatcaaagctggtgctccttgcagaagcgagcgactggccaagtataaccagttgcttcgaattgaggaagagcttggggacgatgcattttatgccggtgaagagtggagggaatcaccctga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005794 GO:0006950 GO:0006979 GO:0008150 GO:0009987 GO:0012505 GO:0016020 GO:0019725 GO:0031090 GO:0033554 GO:0034599 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050896 GO:0051716 GO:0055082 GO:0055088 GO:0065007 GO:0065008 GO:0070887 GO:0090156 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko00600 ko01100 ko04071 map00600 map01100 map04071

Pfam Domains

Protein Families

Ceramidase_alk Ceramidse_alk_C

Protein Analysis

1291

Amino Acids

142.09

Weight (kDa)

7.71

Isoelectric Point (pI)

39.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ceramidase_alk	PF04734	37 - 608	1.4e-228	Neutral/alkaline non-lysosomal ceramidase, N-terminal
Ceramidse_alk_C	PF17048	610 - 767	4.1e-50	Neutral/alkaline non-lysosomal ceramidase, C-terminal
Enolase_N	PF03952	859 - 992	6.7e-49	Enolase, N-terminal domain
Enolase_C	PF00113	1001 - 1288	2.9e-117	Enolase, C-terminal TIM barrel domain
MR_MLE_C	PF13378	1131 - 1252	2.6e-07	Enolase C-terminal domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0010845)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07380 AT1G07380 AT5G58980
fragaria_vesca	FvH4_3g37680
malus_domestica	MD03G1079000.v1.1
prunus_persica	Prupe.6G063600_v2.0.a1
pyrus_communis	pycom03g06300
rosa_chinensis	RchiOBHm_Chr5g0067951
rosa_laevigata	RLG00000035946
rosa_multiflora	Rmu_sc0000435.1_g000039
rosa_roxburghii	Rroxscaffold_1G00012650 Rroxscaffold_1G00013360
rosa_rugosa	Rorug05G0386600 Rorug05G0386700
rosa_samantha	Rh5AG445600 Rh5BG463100 Rh5CG485000 Rh5DG477100
rosa_wichuraiana	Rw0G015370 Rw5G041610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3032
Acc16I	TGCGCA	1 cut(s)	1451
AccB1I	GGYRCC	2 cut(s)	1549, 2258
AccB7I	CCANNNNNTGG	1 cut(s)	2205
AccBSI	CCGCTC	1 cut(s)	92
AccI	GTMKAC	2 cut(s)	2238, 3165
AccII	CGCG	1 cut(s)	850
AccIII	TCCGGA	1 cut(s)	1578
AciI	CCGC	9 cut(s)	92, 149, 239, 848, 1639, 2223, 2502, 2520, 3110
AcoI	YGGCCR	2 cut(s)	1141, 3789
AcsI	RAATTY	7 cut(s)	21, 378, 1166, 1616, 2211, 2616, 3547
AcuI	CTGAAG	6 cut(s)	674, 2300, 2652, 3623, 3651, 3726
AcyI	GRCGYC	2 cut(s)	1550, 2490
AdeI	CACNNNGTG	1 cut(s)	1702
AfiI	CCNNNNNNNGG	9 cut(s)	1119, 1150, 1313, 1314, 2065, 2104, 2205, 2296, 2508
AflIII	ACRYGT	4 cut(s)	970, 1178, 1340, 3584
AhlI	ACTAGT	1 cut(s)	1670
AjiI	CACGTC	1 cut(s)	971
AjuI	GAANNNNNNNTTGG	6 cut(s)	2095, 2127, 2796, 2828, 2891, 2923
Alw21I	GWGCWC	3 cut(s)	223, 2307, 3769
Alw26I	GTCTC	2 cut(s)	930, 3743
AlwNI	CAGNNNCTG	3 cut(s)	1082, 1113, 3629
Ama87I	CYCGRG	2 cut(s)	122, 440
Aor13HI	TCCGGA	1 cut(s)	1578
AoxI	GGCC	8 cut(s)	288, 394, 1141, 1471, 2511, 2715, 3474, 3789
ApoI	RAATTY	7 cut(s)	21, 378, 1166, 1616, 2211, 2616, 3547
ArsI	GACNNNNNNTTYG	6 cut(s)	615, 647, 1474, 1506, 2478, 2510
AseI	ATTAAT	1 cut(s)	740
AspLEI	GCGC	6 cut(s)	520, 612, 1452, 1552, 2151, 3179
AsuC2I	CCSGG	3 cut(s)	1152, 1249, 2232
AsuII	TTCGAA	1 cut(s)	3811
AsuNHI	GCTAGC	1 cut(s)	1705
AvaI	CYCGRG	2 cut(s)	122, 440
AvaII	GGWCC	8 cut(s)	143, 677, 951, 1148, 1388, 1796, 1877, 2443
BaeI	ACNNNNGTAYC	2 cut(s)	2100, 2133
BalI	TGGCCA	1 cut(s)	3791
BamHI	GGATCC	1 cut(s)	2821
BanI	GGYRCC	2 cut(s)	1549, 2258
BanII	GRGCYC	4 cut(s)	123, 223, 1052, 2307
BbsI	GAAGAC	2 cut(s)	789, 2493
Bbv12I	GWGCWC	3 cut(s)	223, 2307, 3769
BccI	CCATC	9 cut(s)	160, 470, 885, 1379, 1931, 2726, 2812, 2914, 3197
BceAI	ACGGC	4 cut(s)	2313, 2477, 2489, 2702
BcgI	CGANNNNNNTGC	2 cut(s)	78, 112
BciVI	GTATCC	1 cut(s)	2101
BclI	TGATCA	1 cut(s)	2845
BcnI	CCSGG	3 cut(s)	1152, 1249, 2232
BcoDI	GTCTC	2 cut(s)	930, 3743
BcuI	ACTAGT	1 cut(s)	1670
BfaI	CTAG	8 cut(s)	579, 1005, 1061, 1671, 1706, 2184, 2706, 3062
BfmI	CTRYAG	3 cut(s)	410, 600, 1842
BfoI	RGCGCY	1 cut(s)	1553
BfuI	GTATCC	1 cut(s)	2101
BglI	GCCNNNNNGGC	3 cut(s)	2374, 2510, 2909
BglII	AGATCT	2 cut(s)	1572, 1891
BlpI	GCTNAGC	1 cut(s)	2301
BmcAI	AGTACT	1 cut(s)	2182
Bme18I	GGWCC	8 cut(s)	143, 677, 951, 1148, 1388, 1796, 1877, 2443
BmeT110I	CYCGRG	2 cut(s)	122, 440
BmgBI	CACGTC	1 cut(s)	971
BmrI	ACTGGG	3 cut(s)	1092, 1555, 3679
BmtI	GCTAGC	1 cut(s)	1709
BmuI	ACTGGG	3 cut(s)	1092, 1555, 3679
BpiI	GAAGAC	2 cut(s)	789, 2493
BplI	GAGNNNNNCTC	2 cut(s)	2200, 2232
BpmI	CTGGAG	7 cut(s)	582, 923, 1413, 1459, 1632, 1908, 3211
Bpu1102I	GCTNAGC	1 cut(s)	2301
Bpu14I	TTCGAA	1 cut(s)	3811
BpuEI	CTTGAG	2 cut(s)	976, 2399
BpuMI	CCSGG	3 cut(s)	1152, 1249, 2232
Bsa29I	ATCGAT	1 cut(s)	2282
BsaAI	YACGTR	2 cut(s)	1181, 3585
BsaBI	GATNNNNATC	1 cut(s)	1101
BsaHI	GRCGYC	2 cut(s)	1550, 2490
BsaI	GGTCTC	1 cut(s)	3743
BsaJI	CCNNGG	7 cut(s)	397, 398, 1144, 2048, 2098, 2199, 3477
BsaWI	WCCGGW	3 cut(s)	140, 1578, 3288
Bsc4I	CCNNNNNNNGG	9 cut(s)	1119, 1150, 1313, 1314, 2065, 2104, 2205, 2296, 2508
Bse118I	RCCGGY	2 cut(s)	145, 3848
Bse3DI	GCAATG	4 cut(s)	1359, 1459, 2859, 2973
Bse8I	GATNNNNATC	1 cut(s)	1101
BseAI	TCCGGA	1 cut(s)	1578
BseCI	ATCGAT	1 cut(s)	2282
BseDI	CCNNGG	7 cut(s)	397, 398, 1144, 2048, 2098, 2199, 3477
BseGI	GGATG	8 cut(s)	914, 1423, 1781, 1942, 1984, 2823, 3191, 3667
BseJI	GATNNNNATC	1 cut(s)	1101
BseLI	CCNNNNNNNGG	9 cut(s)	1119, 1150, 1313, 1314, 2065, 2104, 2205, 2296, 2508
BseMI	GCAATG	4 cut(s)	1359, 1459, 2859, 2973
BseRI	GAGGAG	2 cut(s)	809, 918
BseYI	CCCAGC	3 cut(s)	1287, 2936, 3050
BsgI	GTGCAG	3 cut(s)	753, 1057, 1784
Bsh1236I	CGCG	1 cut(s)	850
BshFI	GGCC	8 cut(s)	290, 396, 1143, 1473, 2513, 2717, 3476, 3791
BshNI	GGYRCC	2 cut(s)	1549, 2258
BshVI	ATCGAT	1 cut(s)	2282
BsiHKAI	GWGCWC	3 cut(s)	223, 2307, 3769
BsiHKCI	CYCGRG	2 cut(s)	122, 440
BsiSI	CCGG	9 cut(s)	141, 146, 198, 1151, 1248, 1579, 2232, 3289, 3849
BslFI	GGGAC	2 cut(s)	3634, 3845
BslI	CCNNNNNNNGG	9 cut(s)	1119, 1150, 1313, 1314, 2065, 2104, 2205, 2296, 2508
BsmAI	GTCTC	2 cut(s)	930, 3743
BsmFI	GGGAC	2 cut(s)	3634, 3845
BsmI	GAATGC	1 cut(s)	2905
BsnI	GGCC	8 cut(s)	290, 396, 1143, 1473, 2513, 2717, 3476, 3791
Bso31I	GGTCTC	1 cut(s)	3743
BsoBI	CYCGRG	2 cut(s)	122, 440
Bsp119I	TTCGAA	1 cut(s)	3811
Bsp1286I	GDGCHC	5 cut(s)	123, 223, 1052, 2307, 3769
Bsp13I	TCCGGA	1 cut(s)	1578
Bsp1407I	TGTACA	1 cut(s)	2964
Bsp1720I	GCTNAGC	1 cut(s)	2301
Bsp19I	CCATGG	2 cut(s)	2098, 2199
BspACI	CCGC	9 cut(s)	92, 149, 239, 848, 1639, 2223, 2502, 2520, 3110
BspANI	GGCC	8 cut(s)	290, 396, 1143, 1473, 2513, 2717, 3476, 3791
BspDI	ATCGAT	1 cut(s)	2282
BspEI	TCCGGA	1 cut(s)	1578
BspFNI	CGCG	1 cut(s)	850
BspHI	TCATGA	2 cut(s)	880, 2011
BspMAI	CTGCAG	1 cut(s)	604
BspOI	GCTAGC	1 cut(s)	1709
BspQI	GCTCTTC	2 cut(s)	3600, 3816
BspT104I	TTCGAA	1 cut(s)	3811
BspT107I	GGYRCC	2 cut(s)	1549, 2258
BspTNI	GGTCTC	1 cut(s)	3743
BsrBI	CCGCTC	1 cut(s)	92
BsrDI	GCAATG	4 cut(s)	1359, 1459, 2859, 2973
BsrFI	RCCGGY	2 cut(s)	145, 3848
BsrGI	TGTACA	1 cut(s)	2964
BssAI	RCCGGY	2 cut(s)	145, 3848
BssECI	CCNNGG	7 cut(s)	397, 398, 1144, 2048, 2098, 2199, 3477
BssNAI	GTATAC	1 cut(s)	2239
BssNI	GRCGYC	2 cut(s)	1550, 2490
BssT1I	CCWWGG	2 cut(s)	2098, 2199
Bst1107I	GTATAC	1 cut(s)	2239
Bst6I	CTCTTC	8 cut(s)	654, 847, 1746, 2540, 3600, 3702, 3816, 3849
BstACI	GRCGYC	2 cut(s)	1550, 2490
BstAPI	GCANNNNNTGC	2 cut(s)	3662, 3845
BstAUI	TGTACA	1 cut(s)	2964
BstBAI	YACGTR	2 cut(s)	1181, 3585
BstBI	TTCGAA	1 cut(s)	3811
BstDSI	CCRYGG	2 cut(s)	2098, 2199
BstEII	GGTNACC	1 cut(s)	1154
BstF5I	GGATG	8 cut(s)	914, 1423, 1781, 1942, 1984, 2823, 3191, 3667
BstFNI	CGCG	1 cut(s)	850
BstH2I	RGCGCY	1 cut(s)	1553
BstHHI	GCGC	6 cut(s)	520, 612, 1452, 1552, 2151, 3179
BstMAI	GTCTC	2 cut(s)	930, 3743
BstNSI	RCATGY	2 cut(s)	1344, 2659
BstPI	GGTNACC	1 cut(s)	1154
BstSFI	CTRYAG	3 cut(s)	410, 600, 1842
BstUI	CGCG	1 cut(s)	850
BstV2I	GAAGAC	2 cut(s)	789, 2493
BstX2I	RGATCY	8 cut(s)	343, 1216, 1572, 1891, 2598, 2821, 3247, 3457
BstXI	CCANNNNNNTGG	1 cut(s)	1545
BstYI	RGATCY	8 cut(s)	343, 1216, 1572, 1891, 2598, 2821, 3247, 3457
BstZ17I	GTATAC	1 cut(s)	2239
Bsu15I	ATCGAT	1 cut(s)	2282
BsuI	GTATCC	1 cut(s)	2101
BsuRI	GGCC	8 cut(s)	290, 396, 1143, 1473, 2513, 2717, 3476, 3791
BsuTUI	ATCGAT	1 cut(s)	2282
BtgI	CCRYGG	2 cut(s)	2098, 2199
BtrI	CACGTC	1 cut(s)	971
BtsCI	GGATG	8 cut(s)	914, 1423, 1781, 1942, 1984, 2823, 3191, 3667
BtsIMutI	CAGTG	8 cut(s)	351, 687, 1380, 1609, 2577, 2960, 3630, 3667
CaiI	CAGNNNCTG	3 cut(s)	1082, 1113, 3629
CciI	TCATGA	2 cut(s)	880, 2011
CfoI	GCGC	6 cut(s)	520, 612, 1452, 1552, 2151, 3179
Cfr10I	RCCGGY	2 cut(s)	145, 3848
ClaI	ATCGAT	1 cut(s)	2282
CpoI	CGGWCCG	1 cut(s)	143
CseI	GACGC	2 cut(s)	440, 2498
CsiI	ACCWGGT	1 cut(s)	947
CspI	CGGWCCG	1 cut(s)	143
DinI	GGCGCC	1 cut(s)	1551
DraIII	CACNNNGTG	1 cut(s)	1702
EaeI	YGGCCR	2 cut(s)	1141, 3789
Eam1104I	CTCTTC	8 cut(s)	654, 847, 1746, 2540, 3600, 3702, 3816, 3849
EarI	CTCTTC	8 cut(s)	654, 847, 1746, 2540, 3600, 3702, 3816, 3849
EciI	GGCGGA	1 cut(s)	254
Ecl136II	GAGCTC	2 cut(s)	221, 2305
Eco130I	CCWWGG	2 cut(s)	2098, 2199
Eco147I	AGGCCT	1 cut(s)	3476
Eco24I	GRGCYC	4 cut(s)	123, 223, 1052, 2307
Eco31I	GGTCTC	1 cut(s)	3743
Eco47I	GGWCC	8 cut(s)	143, 677, 951, 1148, 1388, 1796, 1877, 2443
Eco53kI	GAGCTC	2 cut(s)	221, 2305
Eco57I	CTGAAG	6 cut(s)	674, 2300, 2652, 3623, 3651, 3726
Eco88I	CYCGRG	2 cut(s)	122, 440
Eco91I	GGTNACC	1 cut(s)	1154
EcoICRI	GAGCTC	2 cut(s)	221, 2305
EcoO109I	RGGNCCY	1 cut(s)	951
EcoO65I	GGTNACC	1 cut(s)	1154
EcoRI	GAATTC	4 cut(s)	21, 378, 2211, 2616
EcoT14I	CCWWGG	2 cut(s)	2098, 2199
EcoT22I	ATGCAT	1 cut(s)	3841
EcoT38I	GRGCYC	4 cut(s)	123, 223, 1052, 2307
EgeI	GGCGCC	1 cut(s)	1551
EheI	GGCGCC	1 cut(s)	1551
ErhI	CCWWGG	2 cut(s)	2098, 2199
FalI	AAGNNNNNCTT	6 cut(s)	1287, 1319, 1493, 1525, 2622, 2654
FaqI	GGGAC	2 cut(s)	3634, 3845
FauNDI	CATATG	2 cut(s)	1540, 2550
FbaI	TGATCA	1 cut(s)	2845
FblI	GTMKAC	2 cut(s)	2238, 3165
FokI	GGATG	8 cut(s)	901, 1430, 1768, 1949, 1991, 2830, 3198, 3674
FriOI	GRGCYC	4 cut(s)	123, 223, 1052, 2307
FspBI	CTAG	8 cut(s)	579, 1005, 1061, 1671, 1706, 2184, 2706, 3062
FspI	TGCGCA	1 cut(s)	1451
GlaI	GCGC	6 cut(s)	519, 611, 1451, 1551, 2150, 3178
GsaI	CCCAGC	3 cut(s)	1291, 2940, 3054
GsuI	CTGGAG	7 cut(s)	582, 923, 1413, 1459, 1632, 1908, 3211
HaeII	RGCGCY	1 cut(s)	1553
HaeIII	GGCC	8 cut(s)	290, 396, 1143, 1473, 2513, 2717, 3476, 3791
HapII	CCGG	9 cut(s)	141, 146, 198, 1151, 1248, 1579, 2232, 3289, 3849
HgaI	GACGC	2 cut(s)	440, 2498
HhaI	GCGC	6 cut(s)	520, 612, 1452, 1552, 2151, 3179
Hin1I	GRCGYC	2 cut(s)	1550, 2490
Hin6I	GCGC	6 cut(s)	518, 610, 1450, 1550, 2149, 3177
HinP1I	GCGC	6 cut(s)	518, 610, 1450, 1550, 2149, 3177
HincII	GTYRAC	1 cut(s)	1245
HindII	GTYRAC	1 cut(s)	1245
HindIII	AAGCTT	2 cut(s)	1835, 1912
HpaII	CCGG	9 cut(s)	141, 146, 198, 1151, 1248, 1579, 2232, 3289, 3849
HpyCH4IV	ACGT	6 cut(s)	864, 970, 1180, 1698, 3556, 3584
HpySE526I	ACGT	6 cut(s)	864, 970, 1180, 1698, 3556, 3584
Hsp92I	GRCGYC	2 cut(s)	1550, 2490
HspAI	GCGC	6 cut(s)	518, 610, 1450, 1550, 2149, 3177
KasI	GGCGCC	1 cut(s)	1549
Kpn2I	TCCGGA	1 cut(s)	1578
Ksp22I	TGATCA	1 cut(s)	2845
LguI	GCTCTTC	2 cut(s)	3600, 3816
MabI	ACCWGGT	1 cut(s)	947
MaeI	CTAG	8 cut(s)	579, 1005, 1061, 1671, 1706, 2184, 2706, 3062
MaeII	ACGT	6 cut(s)	864, 970, 1180, 1698, 3556, 3584
MaeIII	GTNAC	8 cut(s)	430, 897, 1154, 1297, 1856, 2020, 2026, 3625
MbiI	CCGCTC	1 cut(s)	92
MfeI	CAATTG	5 cut(s)	694, 3090, 3303, 3441, 3450
MflI	RGATCY	8 cut(s)	343, 1216, 1572, 1891, 2598, 2821, 3247, 3457
MhlI	GDGCHC	5 cut(s)	123, 223, 1052, 2307, 3769
MlsI	TGGCCA	1 cut(s)	3791
MluNI	TGGCCA	1 cut(s)	3791
Mly113I	GGCGCC	1 cut(s)	1550
MlyI	GAGTC	4 cut(s)	640, 733, 1504, 3719
MmeI	TCCRAC	6 cut(s)	129, 707, 2257, 2332, 2350, 3505
Mox20I	TGGCCA	1 cut(s)	3791
Mph1103I	ATGCAT	1 cut(s)	3841
MroI	TCCGGA	1 cut(s)	1578
MscI	TGGCCA	1 cut(s)	3791
MslI	CAYNNNNRTG	4 cut(s)	387, 2256, 3184, 3693
Msp20I	TGGCCA	1 cut(s)	3791
MspI	CCGG	9 cut(s)	141, 146, 198, 1151, 1248, 1579, 2232, 3289, 3849
MunI	CAATTG	5 cut(s)	694, 3090, 3303, 3441, 3450
Mva1269I	GAATGC	1 cut(s)	2905
MvnI	CGCG	1 cut(s)	850
NarI	GGCGCC	1 cut(s)	1550
NciI	CCSGG	3 cut(s)	1152, 1249, 2232
NcoI	CCATGG	2 cut(s)	2098, 2199
NdeI	CATATG	2 cut(s)	1540, 2550
NheI	GCTAGC	1 cut(s)	1705
NmeAIII	GCCGAG	2 cut(s)	1169, 2572
NmuCI	GTSAC	1 cut(s)	3625
NsbI	TGCGCA	1 cut(s)	1451
NsiI	ATGCAT	1 cut(s)	3841
NspI	RCATGY	2 cut(s)	1344, 2659
NspV	TTCGAA	1 cut(s)	3811
PagI	TCATGA	2 cut(s)	880, 2011
PasI	CCCWGGG	1 cut(s)	398
PceI	AGGCCT	1 cut(s)	3476
PciI	ACATGT	1 cut(s)	1340
PciSI	GCTCTTC	2 cut(s)	3600, 3816
PcsI	WCGNNNNNNNCGW	1 cut(s)	2490
PctI	GAATGC	1 cut(s)	2905
PfeI	GAWTC	9 cut(s)	101, 271, 2092, 2284, 2545, 2563, 3082, 3578, 3866
PflFI	GACNNNGTC	1 cut(s)	2492
PflMI	CCANNNNNTGG	1 cut(s)	2205
PleI	GAGTC	4 cut(s)	640, 732, 1503, 3718
PluTI	GGCGCC	1 cut(s)	1553
PpsI	GAGTC	4 cut(s)	640, 732, 1503, 3718
Ppu21I	YACGTR	2 cut(s)	1181, 3585
PpuMI	RGGWCCY	1 cut(s)	951
PscI	ACATGT	1 cut(s)	1340
PshBI	ATTAAT	1 cut(s)	740
PsiI	TTATAA	1 cut(s)	3032
Psp124BI	GAGCTC	2 cut(s)	223, 2307
Psp5II	RGGWCCY	1 cut(s)	951
PspEI	GGTNACC	1 cut(s)	1154
PspFI	CCCAGC	3 cut(s)	1287, 2936, 3050
PspPPI	RGGWCCY	1 cut(s)	951
PstI	CTGCAG	1 cut(s)	604
PstNI	CAGNNNCTG	3 cut(s)	1082, 1113, 3629
PsuI	RGATCY	8 cut(s)	343, 1216, 1572, 1891, 2598, 2821, 3247, 3457
PsyI	GACNNNGTC	1 cut(s)	2492
RseI	CAYNNNNRTG	4 cut(s)	387, 2256, 3184, 3693
Rsr2I	CGGWCCG	1 cut(s)	143
RsrII	CGGWCCG	1 cut(s)	143
SacI	GAGCTC	2 cut(s)	223, 2307
SapI	GCTCTTC	2 cut(s)	3600, 3816
ScaI	AGTACT	1 cut(s)	2182
SchI	GAGTC	4 cut(s)	640, 733, 1504, 3719
SduI	GDGCHC	5 cut(s)	123, 223, 1052, 2307, 3769
SexAI	ACCWGGT	1 cut(s)	947
SfcI	CTRYAG	3 cut(s)	410, 600, 1842
SfoI	GGCGCC	1 cut(s)	1551
SfuI	TTCGAA	1 cut(s)	3811
SinI	GGWCC	8 cut(s)	143, 677, 951, 1148, 1388, 1796, 1877, 2443
SmiMI	CAYNNNNRTG	4 cut(s)	387, 2256, 3184, 3693
SmlI	CTYRAG	2 cut(s)	955, 2378
SmoI	CTYRAG	2 cut(s)	955, 2378
SpeI	ACTAGT	1 cut(s)	1670
SseBI	AGGCCT	1 cut(s)	3476
SsiI	CCGC	9 cut(s)	92, 149, 239, 848, 1639, 2223, 2502, 2520, 3110
SspDI	GGCGCC	1 cut(s)	1549
SspI	AATATT	3 cut(s)	1693, 2344, 2358
SspMI	CTAG	8 cut(s)	579, 1005, 1061, 1671, 1706, 2184, 2706, 3062
SstI	GAGCTC	2 cut(s)	223, 2307
StuI	AGGCCT	1 cut(s)	3476
StyI	CCWWGG	2 cut(s)	2098, 2199
TaiI	ACGT	6 cut(s)	867, 973, 1183, 1701, 3559, 3587
TaqI	TCGA	9 cut(s)	633, 663, 2282, 2361, 2484, 2543, 2604, 2918, 3811
TaqII	GACCGA	1 cut(s)	2431
TatI	WGTACW	7 cut(s)	77, 982, 1175, 1456, 2180, 2964, 3428
TauI	GCSGC	4 cut(s)	152, 1642, 2504, 2522
TfiI	GAWTC	9 cut(s)	101, 271, 2092, 2284, 2545, 2563, 3082, 3578, 3866
TscAI	CASTG	8 cut(s)	358, 687, 1387, 1609, 2577, 2967, 3630, 3674
TseFI	GTSAC	1 cut(s)	3625
Tsp45I	GTSAC	1 cut(s)	3625
TspDTI	ATGAA	8 cut(s)	194, 220, 522, 1179, 1544, 2527, 2630, 3575
TspGWI	ACGGA	5 cut(s)	197, 1634, 2081, 2346, 3450
TspRI	CASTG	8 cut(s)	358, 687, 1387, 1609, 2577, 2967, 3630, 3674
Tth111I	GACNNNGTC	1 cut(s)	2492
Van91I	CCANNNNNTGG	1 cut(s)	2205
VpaK11BI	GGWCC	8 cut(s)	143, 677, 951, 1148, 1388, 1796, 1877, 2443
VspI	ATTAAT	1 cut(s)	740
XapI	RAATTY	7 cut(s)	21, 378, 1166, 1616, 2211, 2616, 3547
XceI	RCATGY	2 cut(s)	1344, 2659
XcmI	CCANNNNNNNNNTGG	1 cut(s)	395
XmiI	GTMKAC	2 cut(s)	2238, 3165
XspI	CTAG	8 cut(s)	579, 1005, 1061, 1671, 1706, 2184, 2706, 3062
ZrmI	AGTACT	1 cut(s)	2182
Zsp2I	ATGCAT	1 cut(s)	3841

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.