Rmu_sc0000494.1_g000014

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000494.1
OG0010627
Physical Location & Seq
Forward (+)
65726 .. 66388
663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000494.1_g000014.1.cds

Sequence Viewer

Length: 663 bp
atggtgaagcttaaggggggtggaggtgctgtaactcagcctcatagctccggtgaacttaccagtgtggaatttgacctccacgtccatagagatgaaacggcggccaagttttgcgctttgcaggactcggtctcggaaatcctagaagaactcagatccatgaaagcggaggctaagaacctacatatccagcaaggttcacctcagctgaacgttggatccatacaaatccctgttggttcagcaaggggtagctctatggggacatcggcctcgtcgactatggctacgtcggcttctattcaagttgaaggagggggtagcacatctaaactcaatcattctagctctagttttcctttgaacttaaatgataatgatgtgacatttgggtttgctagtggtaactatgctgaaatgattaaggctaccaatcttatgcaaacaactagtattgatgtgagtggatctgatacaactaataaattggatcgtaaagttgttggatgcatgggacctagcagcttgtcacatcatgaacaaaaccaattttcacaacatgctatttctggacatggtaacacccaaggttctaaatctctgcgatattgccgatatatcccccgatatctccttttttcgacggaccgatatatttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

220

Amino Acids

23.47

Weight (kDa)

6.75

Isoelectric Point (pI)

31.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 83
AccI GTMKAC 1 cut(s) 281
AciI CCGC 2 cut(s) 104, 170
AclI AACGTT 1 cut(s) 216
AclWI GGATC 5 cut(s) 153, 216, 229, 478, 501
AcoI YGGCCR 1 cut(s) 105
AcsI RAATTY 1 cut(s) 71
AflII CTTAAG 1 cut(s) 11
AgsI TTSAA 3 cut(s) 308, 314, 367
AhlI ACTAGT 1 cut(s) 452
AjiI CACGTC 1 cut(s) 85
AloI GAACNNNNNNTCC 2 cut(s) 206, 238
AluBI AGCT 6 cut(s) 10, 48, 211, 258, 351, 528
AluI AGCT 6 cut(s) 10, 48, 211, 258, 351, 528
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 5 cut(s) 153, 216, 229, 478, 501
AoxI GGCC 2 cut(s) 105, 273
ApeKI GCWGC 1 cut(s) 525
ApoI RAATTY 1 cut(s) 71
AspLEI GCGC 1 cut(s) 119
AspS9I GGNCC 2 cut(s) 518, 649
AsuHPI GGTGA 3 cut(s) 16, 65, 195
AvaII GGWCC 2 cut(s) 518, 649
BamHI GGATCC 1 cut(s) 221
BbvCI CCTCAGC 1 cut(s) 207
BbvI GCAGC 1 cut(s) 537
BceAI ACGGC 1 cut(s) 117
BcoDI GTCTC 1 cut(s) 139
BcuI ACTAGT 1 cut(s) 452
BfaI CTAG 6 cut(s) 146, 348, 354, 402, 453, 522
BfrI CTTAAG 1 cut(s) 11
BisI GCNGC 2 cut(s) 105, 526
BlsI GCNGC 2 cut(s) 106, 527
Bme18I GGWCC 2 cut(s) 518, 649
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 2 cut(s) 518, 649
BmiI GGNNCC 2 cut(s) 223, 519
BmsI GCATC 1 cut(s) 500
Bpu10I CCTNAGC 1 cut(s) 207
BsaI GGTCTC 1 cut(s) 139
BsaJI CCNNGG 1 cut(s) 589
BsaWI WCCGGW 1 cut(s) 50
BsaXI ACNNNNNCTCC 2 cut(s) 15, 45
Bse1I ACTGG 1 cut(s) 63
BseDI CCNNGG 1 cut(s) 589
BseGI GGATG 1 cut(s) 515
BseMII CTCAG 3 cut(s) 50, 169, 221
BseNI ACTGG 1 cut(s) 63
BseXI GCAGC 1 cut(s) 537
BshFI GGCC 2 cut(s) 107, 275
BsiSI CCGG 1 cut(s) 51
BslFI GGGAC 2 cut(s) 280, 531
BsmAI GTCTC 1 cut(s) 139
BsmFI GGGAC 2 cut(s) 280, 531
BsnI GGCC 2 cut(s) 107, 275
Bso31I GGTCTC 1 cut(s) 139
Bsp143I GATC 4 cut(s) 158, 221, 470, 493
BspACI CCGC 2 cut(s) 104, 170
BspANI GGCC 2 cut(s) 107, 275
BspCNI CTCAG 3 cut(s) 49, 168, 220
BspHI TCATGA 1 cut(s) 538
BspLI GGNNCC 2 cut(s) 223, 519
BspPI GGATC 5 cut(s) 153, 216, 229, 478, 501
BspTI CTTAAG 1 cut(s) 11
BspTNI GGTCTC 1 cut(s) 139
BsrI ACTGG 1 cut(s) 63
BssECI CCNNGG 1 cut(s) 589
BssMI GATC 4 cut(s) 158, 221, 470, 493
BssT1I CCWWGG 1 cut(s) 589
BstAFI CTTAAG 1 cut(s) 11
BstDEI CTNAG 4 cut(s) 36, 155, 177, 207
BstF5I GGATG 1 cut(s) 515
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 4 cut(s) 161, 224, 473, 496
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 4 cut(s) 158, 221, 470, 493
BstMWI GCNNNNNNNGC 1 cut(s) 296
BstNSI RCATGY 1 cut(s) 566
BstV1I GCAGC 1 cut(s) 537
BstX2I RGATCY 3 cut(s) 158, 221, 470
BstYI RGATCY 3 cut(s) 158, 221, 470
BsuRI GGCC 2 cut(s) 107, 275
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 1 cut(s) 515
BtsIMutI CAGTG 1 cut(s) 70
CciI TCATGA 1 cut(s) 538
CfoI GCGC 1 cut(s) 119
Cfr13I GGNCC 2 cut(s) 518, 649
CpoI CGGWCCG 1 cut(s) 649
CspI CGGWCCG 1 cut(s) 649
CviAII CATG 5 cut(s) 163, 514, 539, 563, 578
DdeI CTNAG 4 cut(s) 36, 155, 177, 207
DpnI GATC 4 cut(s) 160, 223, 472, 495
DpnII GATC 4 cut(s) 158, 221, 470, 493
DrdI GACNNNNNNGTC 1 cut(s) 83
DseDI GACNNNNNNGTC 1 cut(s) 83
EaeI YGGCCR 1 cut(s) 105
Eco130I CCWWGG 1 cut(s) 589
Eco31I GGTCTC 1 cut(s) 139
Eco32I GATATC 1 cut(s) 632
Eco47I GGWCC 2 cut(s) 518, 649
EcoO109I RGGNCCY 1 cut(s) 518
EcoRV GATATC 1 cut(s) 632
EcoT14I CCWWGG 1 cut(s) 589
EcoT22I ATGCAT 1 cut(s) 515
ErhI CCWWGG 1 cut(s) 589
FaeI CATG 5 cut(s) 166, 517, 542, 566, 581
FaqI GGGAC 2 cut(s) 280, 531
FatI CATG 5 cut(s) 162, 513, 538, 562, 577
FblI GTMKAC 1 cut(s) 281
Fnu4HI GCNGC 2 cut(s) 105, 526
FokI GGATG 1 cut(s) 522
Fsp4HI GCNGC 2 cut(s) 105, 526
FspBI CTAG 6 cut(s) 146, 348, 354, 402, 453, 522
GlaI GCGC 1 cut(s) 118
GluI GCNGC 2 cut(s) 105, 526
HaeIII GGCC 2 cut(s) 107, 275
HapII CCGG 1 cut(s) 51
HhaI GCGC 1 cut(s) 119
Hin1II CATG 5 cut(s) 166, 517, 542, 566, 581
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HincII GTYRAC 1 cut(s) 282
HindII GTYRAC 1 cut(s) 282
HindIII AAGCTT 1 cut(s) 8
HinfI GANTC 1 cut(s) 128
HpaII CCGG 1 cut(s) 51
HphI GGTGA 3 cut(s) 16, 65, 195
Hpy166II GTNNAC 3 cut(s) 56, 203, 282
Hpy188I TCNGA 3 cut(s) 139, 158, 475
Hpy188III TCNNGA 2 cut(s) 539, 573
Hpy8I GTNNAC 3 cut(s) 56, 203, 282
Hpy99I CGWCG 3 cut(s) 283, 298, 649
HpyAV CCTTC 1 cut(s) 308
HpyCH4IV ACGT 3 cut(s) 84, 216, 293
HpyCH4V TGCA 3 cut(s) 124, 445, 513
HpyF10VI GCNNNNNNNGC 1 cut(s) 296
HpyF3I CTNAG 4 cut(s) 36, 155, 177, 207
HpySE526I ACGT 3 cut(s) 84, 216, 293
Hsp92II CATG 5 cut(s) 166, 517, 542, 566, 581
HspAI GCGC 1 cut(s) 117
Kzo9I GATC 4 cut(s) 158, 221, 470, 493
LmnI GCTCC 1 cut(s) 53
LpnPI CCDG 6 cut(s) 64, 76, 110, 206, 249, 558
Lsp1109I GCAGC 1 cut(s) 537
LweI GCATC 1 cut(s) 500
MaeI CTAG 6 cut(s) 146, 348, 354, 402, 453, 522
MaeII ACGT 3 cut(s) 84, 216, 293
MaeIII GTNAC 5 cut(s) 31, 385, 407, 531, 581
MalI GATC 4 cut(s) 160, 223, 472, 495
MboI GATC 4 cut(s) 158, 221, 470, 493
MboII GAAGA 1 cut(s) 161
MflI RGATCY 3 cut(s) 158, 221, 470
MluCI AATT 3 cut(s) 71, 488, 551
MlyI GAGTC 1 cut(s) 122
MmeI TCCRAC 2 cut(s) 199, 487
MnlI CCTC 7 cut(s) 17, 51, 89, 166, 216, 286, 311
Mph1103I ATGCAT 1 cut(s) 515
MseI TTAA 4 cut(s) 12, 371, 426, 661
MslI CAYNNNNRTG 1 cut(s) 93
MspA1I CMGCKG 1 cut(s) 211
MspCI CTTAAG 1 cut(s) 11
MspI CCGG 1 cut(s) 51
MwoI GCNNNNNNNGC 1 cut(s) 296
NdeII GATC 4 cut(s) 158, 221, 470, 493
NlaIII CATG 5 cut(s) 166, 517, 542, 566, 581
NlaIV GGNNCC 2 cut(s) 223, 519
NmuCI GTSAC 2 cut(s) 385, 531
NsiI ATGCAT 1 cut(s) 515
NspI RCATGY 1 cut(s) 566
PagI TCATGA 1 cut(s) 538
PcsI WCGNNNNNNNCGW 1 cut(s) 278
PflFI GACNNNGTC 1 cut(s) 131
PkrI GCNGC 2 cut(s) 106, 527
PleI GAGTC 1 cut(s) 122
PpsI GAGTC 1 cut(s) 122
PpuMI RGGWCCY 1 cut(s) 518
Psp1406I AACGTT 1 cut(s) 216
Psp5II RGGWCCY 1 cut(s) 518
PspN4I GGNNCC 2 cut(s) 223, 519
PspPI GGNCC 2 cut(s) 518, 649
PspPPI RGGWCCY 1 cut(s) 518
PsuI RGATCY 3 cut(s) 158, 221, 470
PsyI GACNNNGTC 1 cut(s) 131
PvuII CAGCTG 1 cut(s) 211
RseI CAYNNNNRTG 1 cut(s) 93
Rsr2I CGGWCCG 1 cut(s) 649
RsrII CGGWCCG 1 cut(s) 649
SalI GTCGAC 1 cut(s) 280
SaqAI TTAA 4 cut(s) 12, 371, 426, 661
SatI GCNGC 2 cut(s) 105, 526
Sau3AI GATC 4 cut(s) 158, 221, 470, 493
Sau96I GGNCC 2 cut(s) 518, 649
SchI GAGTC 1 cut(s) 122
SfaNI GCATC 1 cut(s) 500
SinI GGWCC 2 cut(s) 518, 649
SmiMI CAYNNNNRTG 1 cut(s) 93
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
SpeI ACTAGT 1 cut(s) 452
Sse9I AATT 3 cut(s) 71, 488, 551
SsiI CCGC 2 cut(s) 104, 170
SspMI CTAG 6 cut(s) 146, 348, 354, 402, 453, 522
StyI CCWWGG 1 cut(s) 589
TaiI ACGT 3 cut(s) 87, 219, 296
TaqI TCGA 2 cut(s) 281, 644
TaqII GACCGA 1 cut(s) 121
TasI AATT 3 cut(s) 71, 488, 551
TauI GCSGC 1 cut(s) 107
Tru1I TTAA 4 cut(s) 12, 371, 426, 661
Tru9I TTAA 4 cut(s) 12, 371, 426, 661
TscAI CASTG 1 cut(s) 70
TseFI GTSAC 2 cut(s) 385, 531
TseI GCWGC 1 cut(s) 525
Tsp45I GTSAC 2 cut(s) 385, 531
TspDTI ATGAA 3 cut(s) 111, 179, 555
TspGWI ACGGA 1 cut(s) 662
TspRI CASTG 1 cut(s) 70
Tth111I GACNNNGTC 1 cut(s) 131
Vha464I CTTAAG 1 cut(s) 11
VpaK11BI GGWCC 2 cut(s) 518, 649
XapI RAATTY 1 cut(s) 71
XceI RCATGY 1 cut(s) 566
XmiI GTMKAC 1 cut(s) 281
XspI CTAG 6 cut(s) 146, 348, 354, 402, 453, 522
Zsp2I ATGCAT 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.