Rmu_sc0000526.1_g000014

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000526.1
OG0015539
Physical Location & Seq
Forward (+)
55139 .. 57656
2518 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1857 bp
atgtcaattgcacaaagagggtgtgattctgaaagagagatttggaagatggagaacggttggtccttaagtgtttctgataatgaagttgctggatcagttcgaacgagattggagtttgaggaaggcagagtgcagggaatggaggagatgaatgttgatactgaggatcgaaacttgacggaggtccctccaatccttcctccagattctaatctcgagattgtatctgctgggggttgtttctcaacctctcattctgattcaaaatctgtgaatcaaaagtcaaaacagaagaaagggatttttgaatttggggaaggcaaatctgtaggggtggaaggggatgatgttagagtttacaaaaggggaggtggaagaggtcggcgaagtgatggcagagagggttttgggggaggaggggggagtactaaggacaaggagaaagggaaggaagttgtctctgaaggtgtggagaatgatgggaagctaaagagaacgaaagatacggcagcattgggaggtcgaggtagaggtggaaaggggcgctatggctttccagtcagcggtcaggggcgtgggcactcctccaaccgagttcccccacgaaaatccaatcttgctgatgtctcaggagttagctggaatgtgcggggattggggaacccgcatacattccgagccttgaagaagtttcttcaagcgaaagaccccgacttggtggtcttaatggaaaccaagaaaaagaaggaggaaatggctgatatttgctttaatttgggttttggtgggtgtagtgtagtggggaaggtgggtcatagtagcggtggtttagccttgtgctggaagagtcagttggtcgtcaggcctttgggcttttcccaagggcatattgatgttgagatacagctgaatggggaaactatcagggtcactggtttttatggaaatccagaaactcatctctggcaattttcttgggaactcttgcgacgaatagctgcttcggtaaggggatcgtggattatctttggggattttaatgaattgctcagtgtttctgagaagattggtgggaatgagaggaagttgagttttcgagcccgatggttacttggagacgtggaaaattgggctgagagtaattctttgggaggcattcaaagaaatctatttactgtttcaaaggctttgaggcgttggaatgatgataatattggccatattccgcggaagataaggcagttgcagggggaggtatccaaatggccttttgataatctagatgacagggtacagcagatgagaaaggaggtattgggtgagtcgaacaggtttatggagcttgaggagtcattgtggaaacagaggtctcaggtgtcgtggttgaatgagggagatagtaacacaaaattctttcacgggtatgcaaagactcggggaagaaaaaacagcatacagggtattactaattcagagggagaatggcaggattctgataagggtattcaggatgctttcttagattacttctcaaagttgtttacttctgaagggtgtaactatatggagctggttctcgatgcggtgccacagaaagttaccaaggatatgaaccagaaacttgaacaaccttttacaaggactgatattgatgatgcattgaagcagatgggtccagacaagtcaccggatgtagatggtttctcagctcgattctatcagacttattgggaggtggttggaaatgatatcagtaagttctgtttgcaggttttgaatgatggagctgagttagctgggctcaatcacacattgttgcctttgattccgaaacttgataatcctatagaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

618

Amino Acids

68.89

Weight (kDa)

7.13

Isoelectric Point (pI)

36.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 213 - 349 8.2e-15 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 722
Acc36I ACCTGC 1 cut(s) 1762
AccB1I GGYRCC 1 cut(s) 1588
AccII CGCG 1 cut(s) 1231
AciI CCGC 7 cut(s) 567, 652, 668, 825, 1229, 1231, 1586
AclWI GGATC 3 cut(s) 103, 177, 1024
AcoI YGGCCR 1 cut(s) 1219
AcsI RAATTY 2 cut(s) 311, 1412
AcuI CTGAAG 2 cut(s) 486, 1572
AfaI GTAC 2 cut(s) 430, 1296
AfiI CCNNNNNNNGG 3 cut(s) 566, 718, 843
AflII CTTAAG 1 cut(s) 67
AjiI CACGTC 1 cut(s) 1123
AjuI GAANNNNNNNTTGG 2 cut(s) 839, 871
AloI GAACNNNNNNTCC 2 cut(s) 47, 79
AluBI AGCT 9 cut(s) 490, 642, 910, 1001, 1345, 1573, 1712, 1790, 1799
AluI AGCT 9 cut(s) 490, 642, 910, 1001, 1345, 1573, 1712, 1790, 1799
Alw26I GTCTC 4 cut(s) 466, 634, 1113, 1377
AlwI GGATC 3 cut(s) 103, 177, 1024
Ama87I CYCGRG 2 cut(s) 218, 1437
AoxI GGCC 3 cut(s) 866, 1219, 1268
ApeKI GCWGC 2 cut(s) 512, 1001
ApoI RAATTY 2 cut(s) 311, 1412
AspLEI GCGC 1 cut(s) 549
AspS9I GGNCC 3 cut(s) 63, 187, 1676
AsuHPI GGTGA 2 cut(s) 1334, 1680
AsuII TTCGAA 1 cut(s) 103
AvaI CYCGRG 2 cut(s) 218, 1437
AvaII GGWCC 3 cut(s) 63, 187, 1676
BaeGI GKGCMC 1 cut(s) 585
BalI TGGCCA 1 cut(s) 1221
BanI GGYRCC 1 cut(s) 1588
BanII GRGCYC 2 cut(s) 1105, 1806
BbvI GCAGC 2 cut(s) 524, 988
BccI CCATC 7 cut(s) 43, 389, 476, 1101, 1666, 1694, 1778
BceAI ACGGC 1 cut(s) 525
BciVI GTATCC 1 cut(s) 1270
BcoDI GTCTC 4 cut(s) 466, 634, 1113, 1377
BfaI CTAG 1 cut(s) 1283
BfmI CTRYAG 2 cut(s) 330, 1848
BfoI RGCGCY 1 cut(s) 550
BfrI CTTAAG 1 cut(s) 67
BfuAI ACCTGC 1 cut(s) 1762
BfuI GTATCC 1 cut(s) 1270
BisI GCNGC 2 cut(s) 513, 1002
BlsI GCNGC 2 cut(s) 514, 1003
BmcAI AGTACT 1 cut(s) 430
Bme18I GGWCC 3 cut(s) 63, 187, 1676
BmeT110I CYCGRG 2 cut(s) 218, 1437
BmgBI CACGTC 1 cut(s) 1123
BmgT120I GGNCC 3 cut(s) 63, 187, 1676
BmiI GGNNCC 4 cut(s) 189, 665, 1590, 1677
BmsI GCATC 3 cut(s) 1504, 1573, 1648
BoxI GACNNNNGTC 1 cut(s) 185
BpmI CTGGAG 1 cut(s) 189
Bpu14I TTCGAA 1 cut(s) 103
BpuEI CTTGAG 1 cut(s) 1367
BsaBI GATNNNNATC 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 1377
BsaJI CCNNGG 3 cut(s) 883, 1229, 1605
BsaWI WCCGGW 1 cut(s) 1690
BsaXI ACNNNNNCTCC 2 cut(s) 1343, 1373
Bsc4I CCNNNNNNNGG 3 cut(s) 566, 718, 843
Bse1I ACTGG 2 cut(s) 560, 940
Bse8I GATNNNNATC 1 cut(s) 213
BseDI CCNNGG 3 cut(s) 883, 1229, 1605
BseGI GGATG 3 cut(s) 352, 1519, 1699
BseJI GATNNNNATC 1 cut(s) 213
BseLI CCNNNNNNNGG 3 cut(s) 566, 718, 843
BseMII CTCAG 8 cut(s) 156, 645, 1053, 1066, 1128, 1388, 1722, 1782
BseNI ACTGG 2 cut(s) 560, 940
BseRI GAGGAG 4 cut(s) 161, 432, 577, 1364
BseSI GKGCMC 1 cut(s) 585
BseXI GCAGC 2 cut(s) 524, 988
BseYI CCCAGC 2 cut(s) 233, 1799
BsgI GTGCAG 1 cut(s) 155
Bsh1236I CGCG 1 cut(s) 1231
BshFI GGCC 3 cut(s) 868, 1221, 1270
BshNI GGYRCC 1 cut(s) 1588
BsiHKCI CYCGRG 2 cut(s) 218, 1437
BsiSI CCGG 1 cut(s) 1691
BslFI GGGAC 1 cut(s) 173
BslI CCNNNNNNNGG 3 cut(s) 566, 718, 843
BsmAI GTCTC 4 cut(s) 466, 634, 1113, 1377
BsmBI CGTCTC 1 cut(s) 1113
BsmFI GGGAC 1 cut(s) 173
BsmI GAATGC 1 cut(s) 1158
BsnI GGCC 3 cut(s) 868, 1221, 1270
Bso31I GGTCTC 1 cut(s) 1377
BsoBI CYCGRG 2 cut(s) 218, 1437
Bsp119I TTCGAA 1 cut(s) 103
Bsp1286I GDGCHC 3 cut(s) 585, 1105, 1806
Bsp143I GATC 3 cut(s) 95, 169, 1016
BspACI CCGC 7 cut(s) 567, 652, 668, 825, 1229, 1231, 1586
BspANI GGCC 3 cut(s) 868, 1221, 1270
BspCNI CTCAG 8 cut(s) 157, 644, 1054, 1065, 1129, 1387, 1721, 1783
BspFNI CGCG 1 cut(s) 1231
BspLI GGNNCC 4 cut(s) 189, 665, 1590, 1677
BspMI ACCTGC 1 cut(s) 1762
BspPI GGATC 3 cut(s) 103, 177, 1024
BspT104I TTCGAA 1 cut(s) 103
BspT107I GGYRCC 1 cut(s) 1588
BspTI CTTAAG 1 cut(s) 67
BspTNI GGTCTC 1 cut(s) 1377
BsrI ACTGG 2 cut(s) 560, 940
BssECI CCNNGG 3 cut(s) 883, 1229, 1605
BssMI GATC 3 cut(s) 95, 169, 1016
BssT1I CCWWGG 2 cut(s) 883, 1605
Bst4CI ACNGT 2 cut(s) 59, 1180
Bst6I CTCTTC 2 cut(s) 373, 842
BstAFI CTTAAG 1 cut(s) 67
BstBI TTCGAA 1 cut(s) 103
BstDSI CCRYGG 1 cut(s) 1229
BstF5I GGATG 3 cut(s) 352, 1519, 1699
BstFNI CGCG 1 cut(s) 1231
BstH2I RGCGCY 1 cut(s) 550
BstHHI GCGC 1 cut(s) 549
BstKTI GATC 3 cut(s) 98, 172, 1019
BstMAI GTCTC 4 cut(s) 466, 634, 1113, 1377
BstMBI GATC 3 cut(s) 95, 169, 1016
BstMWI GCNNNNNNNGC 1 cut(s) 1796
BstPAI GACNNNNGTC 1 cut(s) 185
BstSFI CTRYAG 2 cut(s) 330, 1848
BstSLI GKGCMC 1 cut(s) 585
BstUI CGCG 1 cut(s) 1231
BstV1I GCAGC 2 cut(s) 524, 988
BsuI GTATCC 1 cut(s) 1270
BsuRI GGCC 3 cut(s) 868, 1221, 1270
BtgI CCRYGG 1 cut(s) 1229
BtrI CACGTC 1 cut(s) 1123
BtsCI GGATG 3 cut(s) 352, 1519, 1699
BtsIMutI CAGTG 2 cut(s) 933, 1060
BveI ACCTGC 1 cut(s) 1762
CfoI GCGC 1 cut(s) 549
Cfr13I GGNCC 3 cut(s) 63, 187, 1676
Cfr42I CCGCGG 1 cut(s) 1232
Csp6I GTAC 2 cut(s) 429, 1295
CviQI GTAC 2 cut(s) 429, 1295
DpnI GATC 3 cut(s) 97, 171, 1018
DpnII GATC 3 cut(s) 95, 169, 1016
DrdI GACNNNNNNGTC 1 cut(s) 722
DseDI GACNNNNNNGTC 1 cut(s) 722
EaeI YGGCCR 1 cut(s) 1219
Eam1104I CTCTTC 2 cut(s) 373, 842
EarI CTCTTC 2 cut(s) 373, 842
Eco130I CCWWGG 2 cut(s) 883, 1605
Eco147I AGGCCT 1 cut(s) 868
Eco24I GRGCYC 2 cut(s) 1105, 1806
Eco31I GGTCTC 1 cut(s) 1377
Eco32I GATATC 1 cut(s) 1753
Eco47I GGWCC 3 cut(s) 63, 187, 1676
Eco57I CTGAAG 2 cut(s) 486, 1572
Eco88I CYCGRG 2 cut(s) 218, 1437
EcoO109I RGGNCCY 1 cut(s) 187
EcoRV GATATC 1 cut(s) 1753
EcoT14I CCWWGG 2 cut(s) 883, 1605
EcoT22I ATGCAT 1 cut(s) 1663
EcoT38I GRGCYC 2 cut(s) 1105, 1806
ErhI CCWWGG 2 cut(s) 883, 1605
Esp3I CGTCTC 1 cut(s) 1113
FaqI GGGAC 1 cut(s) 173
FauI CCCGC 2 cut(s) 645, 675
Fnu4HI GCNGC 2 cut(s) 513, 1002
FokI GGATG 3 cut(s) 359, 1526, 1706
FriOI GRGCYC 2 cut(s) 1105, 1806
Fsp4HI GCNGC 2 cut(s) 513, 1002
FspBI CTAG 1 cut(s) 1283
GlaI GCGC 1 cut(s) 548
GluI GCNGC 2 cut(s) 513, 1002
GsaI CCCAGC 2 cut(s) 237, 1803
GsuI CTGGAG 1 cut(s) 189
HaeII RGCGCY 1 cut(s) 550
HaeIII GGCC 3 cut(s) 868, 1221, 1270
HapII CCGG 1 cut(s) 1691
HhaI GCGC 1 cut(s) 549
Hin6I GCGC 1 cut(s) 547
HinP1I GCGC 1 cut(s) 547
HpaII CCGG 1 cut(s) 1691
HphI GGTGA 2 cut(s) 1334, 1680
Hpy166II GTNNAC 2 cut(s) 361, 1545
Hpy188III TCNNGA 9 cut(s) 206, 218, 220, 633, 953, 1283, 1511, 1580, 1679
Hpy8I GTNNAC 2 cut(s) 361, 1545
Hpy99I CGWCG 1 cut(s) 996
HpyAV CCTTC 9 cut(s) 119, 209, 314, 335, 445, 461, 742, 802, 1547
HpyCH4III ACNGT 2 cut(s) 59, 1180
HpyCH4IV ACGT 1 cut(s) 1122
HpyCH4V TGCA 6 cut(s) 11, 136, 1249, 1430, 1661, 1771
HpyF10VI GCNNNNNNNGC 1 cut(s) 1796
HpySE526I ACGT 1 cut(s) 1122
HspAI GCGC 1 cut(s) 547
KspI CCGCGG 1 cut(s) 1232
Kzo9I GATC 3 cut(s) 95, 169, 1016
LmnI GCTCC 3 cut(s) 1342, 1570, 1787
Lsp1109I GCAGC 2 cut(s) 524, 988
LweI GCATC 3 cut(s) 1504, 1573, 1648
MaeI CTAG 1 cut(s) 1283
MaeII ACGT 1 cut(s) 1122
MaeIII GTNAC 6 cut(s) 931, 1110, 1403, 1559, 1600, 1686
MalI GATC 3 cut(s) 97, 171, 1018
MboI GATC 3 cut(s) 95, 169, 1016
MboII GAAGA 9 cut(s) 58, 307, 390, 689, 700, 859, 1078, 1246, 1455
MfeI CAATTG 1 cut(s) 6
MhlI GDGCHC 3 cut(s) 585, 1105, 1806
MlsI TGGCCA 1 cut(s) 1221
MluCI AATT 9 cut(s) 6, 311, 775, 971, 1046, 1129, 1144, 1412, 1471
MluNI TGGCCA 1 cut(s) 1221
MlyI GAGTC 4 cut(s) 859, 1334, 1361, 1429
MmeI TCCRAC 3 cut(s) 615, 1181, 1723
Mox20I TGGCCA 1 cut(s) 1221
Mph1103I ATGCAT 1 cut(s) 1663
MscI TGGCCA 1 cut(s) 1221
MseI TTAA 4 cut(s) 68, 728, 774, 1041
MslI CAYNNNNRTG 2 cut(s) 894, 1425
Msp20I TGGCCA 1 cut(s) 1221
MspA1I CMGCKG 3 cut(s) 567, 910, 1231
MspCI CTTAAG 1 cut(s) 67
MspI CCGG 1 cut(s) 1691
MunI CAATTG 1 cut(s) 6
Mva1269I GAATGC 1 cut(s) 1158
MvnI CGCG 1 cut(s) 1231
MwoI GCNNNNNNNGC 1 cut(s) 1796
NdeII GATC 3 cut(s) 95, 169, 1016
NlaIV GGNNCC 4 cut(s) 189, 665, 1590, 1677
NmuCI GTSAC 2 cut(s) 931, 1686
NsiI ATGCAT 1 cut(s) 1663
NspV TTCGAA 1 cut(s) 103
PaeR7I CTCGAG 1 cut(s) 218
PceI AGGCCT 1 cut(s) 868
PctI GAATGC 1 cut(s) 1158
PfeI GAWTC 7 cut(s) 26, 209, 263, 277, 1493, 1716, 1828
PkrI GCNGC 2 cut(s) 514, 1003
PleI GAGTC 4 cut(s) 858, 1333, 1360, 1429
PpsI GAGTC 4 cut(s) 858, 1333, 1360, 1429
PpuMI RGGWCCY 1 cut(s) 187
PshAI GACNNNNGTC 1 cut(s) 185
Psp5II RGGWCCY 1 cut(s) 187
PspFI CCCAGC 2 cut(s) 233, 1799
PspN4I GGNNCC 4 cut(s) 189, 665, 1590, 1677
PspPI GGNCC 3 cut(s) 63, 187, 1676
PspPPI RGGWCCY 1 cut(s) 187
PsrI GAACNNNNNNTAC 2 cut(s) 490, 522
PvuII CAGCTG 1 cut(s) 910
RsaI GTAC 2 cut(s) 430, 1296
RsaNI GTAC 2 cut(s) 429, 1295
RseI CAYNNNNRTG 2 cut(s) 894, 1425
SacII CCGCGG 1 cut(s) 1232
SaqAI TTAA 4 cut(s) 68, 728, 774, 1041
SatI GCNGC 2 cut(s) 513, 1002
Sau3AI GATC 3 cut(s) 95, 169, 1016
Sau96I GGNCC 3 cut(s) 63, 187, 1676
ScaI AGTACT 1 cut(s) 430
SchI GAGTC 4 cut(s) 859, 1334, 1361, 1429
SduI GDGCHC 3 cut(s) 585, 1105, 1806
SfaNI GCATC 3 cut(s) 1504, 1573, 1648
SfcI CTRYAG 2 cut(s) 330, 1848
Sfr274I CTCGAG 1 cut(s) 218
Sfr303I CCGCGG 1 cut(s) 1232
SfuI TTCGAA 1 cut(s) 103
SgrBI CCGCGG 1 cut(s) 1232
SinI GGWCC 3 cut(s) 63, 187, 1676
SlaI CTCGAG 1 cut(s) 218
SmiMI CAYNNNNRTG 2 cut(s) 894, 1425
SmlI CTYRAG 3 cut(s) 67, 218, 1346
SmoI CTYRAG 3 cut(s) 67, 218, 1346
Sse9I AATT 9 cut(s) 6, 311, 775, 971, 1046, 1129, 1144, 1412, 1471
SseBI AGGCCT 1 cut(s) 868
SsiI CCGC 7 cut(s) 567, 652, 668, 825, 1229, 1231, 1586
SspI AATATT 1 cut(s) 1216
SspMI CTAG 1 cut(s) 1283
StuI AGGCCT 1 cut(s) 868
StyI CCWWGG 2 cut(s) 883, 1605
TaaI ACNGT 2 cut(s) 59, 1180
TaiI ACGT 1 cut(s) 1125
TaqI TCGA 8 cut(s) 103, 172, 219, 526, 1099, 1328, 1581, 1714
TasI AATT 9 cut(s) 6, 311, 775, 971, 1046, 1129, 1144, 1412, 1471
TatI WGTACW 1 cut(s) 428
TfiI GAWTC 7 cut(s) 26, 209, 263, 277, 1493, 1716, 1828
Tru1I TTAA 4 cut(s) 68, 728, 774, 1041
Tru9I TTAA 4 cut(s) 68, 728, 774, 1041
TscAI CASTG 2 cut(s) 940, 1060
TseFI GTSAC 2 cut(s) 931, 1686
TseI GCWGC 2 cut(s) 512, 1001
Tsp45I GTSAC 2 cut(s) 931, 1686
TspDTI ATGAA 4 cut(s) 99, 167, 1059, 1628
TspGWI ACGGA 1 cut(s) 197
TspRI CASTG 2 cut(s) 940, 1060
Vha464I CTTAAG 1 cut(s) 67
VpaK11BI GGWCC 3 cut(s) 63, 187, 1676
XapI RAATTY 2 cut(s) 311, 1412
XbaI TCTAGA 1 cut(s) 1282
XhoI CTCGAG 1 cut(s) 218
XspI CTAG 1 cut(s) 1283
ZrmI AGTACT 1 cut(s) 430
Zsp2I ATGCAT 1 cut(s) 1663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.