Rmu_sc0000666.1_g000028

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000666.1
OG0003313
Physical Location & Seq
Reverse (-)
147098 .. 148904
1807 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1614 bp
atgttaaggaaagaacttcatccagtattactacagaagctcttatacaaccagaacatgggaagtgttcgagaaagagattcatctggtattactactgaagcttttataaaaccagtccataataaggtaaatgctgtgtcacagtcagagggaaatgttaaggaaagagcttcatccagtattactacagaagctcttatacaaccagaacatggtaaggtaaatcctctgtctcagtcagacagaaatgttaaggaaagagactcttctggtgtttctaaagaacagccagcagtgaaggaacccgatagagttgatgaggaaatggaagtgctaccttcacaggactcttctgctcgggagactaactcatcagcaaagaatacaagttcaacagtagatggttgtgtgggtgaaaaacagacaactgatgcctccagctctaatgcaaagccgatgcttcacacgtcagcatcagtagaatctggtcttgaatcactggatggaggctatgtcagtgaaattgaaggtgaaagtatagttgatcagttaaaacgacagattgattatgacaggaaatgcataaaggagttgtataaggaattggaagaagaaaggagtgccgctgccattgctgcaaatcaggcaatggccatgatcacaaagctccaagaagagaaagctgcattccatatggaggcccttcactgcttaagaatgatggaagagcaggctgagtttgacgtagaggcactggagaaagccaatgatctcctggctgagaaggagaaagagatacaggacttggaagcagagctagaattttctaagttagacttccaagatgaatcgataatggagaatccacttgacaaaagttgtgatttgatgggggggattgacccggtggagaatactgttatgccctgtgtaaaaaacgatttcaatgttacttgcaactcaatggctactgaggtatccataggcagtaaccaacctcctttttctgaaactactcttttagagtttgaagatgaaaaattgtacattacaaattgtttgaagagcttggagaggaagctttctgaactttccagtagaggggcttcctccaaaatgcttaatggtgggcattcccaaaagcatatggatgatggacaaaatcaagcagagactcctagaaaagagaggagtctactaaatgatggacaaaatcaagaagagactcctaggaaagggaggagtctattaaatggtcagcttgaagagaatgttctgcatgggcaaaaagatttacatatgtctaatggtagctctgctgctcaagacgggccaactggttgtggtggtggtgatgaattagaatgtgaagacaataatgctgattcccgtggggaaaaagatatcatggatcctggagaaatggatttggttattctggaaaatgaaatctcagaccttaatgataggttggaggcacttgagactgatcatgattttcttgagcacatgctctactctcttcaaaatggagcggaaggactacaatttgttcgagagatagcttatcagctgcaagagttgcggaagatagggacgggtttcagatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

537

Amino Acids

59.56

Weight (kDa)

4.6

Isoelectric Point (pI)

42.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 184 - 274 1.2e-28 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 110
AccB7I CCANNNNNTGG 2 cut(s) 58, 215
AccBSI CCGCTC 1 cut(s) 1538
AccI GTMKAC 1 cut(s) 1198
AciI CCGC 3 cut(s) 627, 1538, 1588
AclWI GGATC 2 cut(s) 1409, 1422
AcoI YGGCCR 1 cut(s) 654
AcsI RAATTY 1 cut(s) 824
AcuI CTGAAG 1 cut(s) 120
AfaI GTAC 1 cut(s) 1049
AfiI CCNNNNNNNGG 6 cut(s) 58, 127, 215, 700, 1104, 1238
AflII CTTAAG 1 cut(s) 715
AflIII ACRYGT 1 cut(s) 468
AgsI TTSAA 8 cut(s) 396, 497, 530, 949, 1034, 1066, 1268, 1529
AjiI CACGTC 1 cut(s) 471
AjnI CCWGG 2 cut(s) 777, 1417
Alw21I GWGCWC 1 cut(s) 1512
Alw26I GTCTC 6 cut(s) 240, 258, 359, 1169, 1220, 1481
AlwI GGATC 2 cut(s) 1409, 1422
Ama87I CYCGRG 1 cut(s) 360
AoxI GGCC 3 cut(s) 654, 702, 1334
ApeKI GCWGC 5 cut(s) 629, 638, 686, 1322, 1576
ApoI RAATTY 1 cut(s) 824
AspA2I CCTAGG 1 cut(s) 1232
AspS9I GGNCC 2 cut(s) 703, 1334
AsuC2I CCSGG 1 cut(s) 908
AsuHPI GGTGA 3 cut(s) 428, 545, 1367
AvaI CYCGRG 1 cut(s) 360
AvrII CCTAGG 1 cut(s) 1232
BaeI ACNNNNGTAYC 2 cut(s) 963, 996
BalI TGGCCA 1 cut(s) 656
BamHI GGATCC 1 cut(s) 1414
BarI GAAGNNNNNNTAC 6 cut(s) 29, 61, 186, 218, 1093, 1125
BbsI GAAGAC 1 cut(s) 1380
Bbv12I GWGCWC 1 cut(s) 1512
BbvI GCAGC 5 cut(s) 616, 625, 673, 1309, 1563
BccI CCATC 6 cut(s) 398, 500, 718, 886, 1151, 1202
BciT130I CCWGG 2 cut(s) 779, 1419
BciVI GTATCC 1 cut(s) 991
BclI TGATCA 3 cut(s) 547, 660, 1492
BcnI CCSGG 1 cut(s) 908
BcoDI GTCTC 6 cut(s) 240, 258, 359, 1169, 1220, 1481
BfaI CTAG 3 cut(s) 821, 1182, 1233
BfmI CTRYAG 2 cut(s) 32, 189
BfrI CTTAAG 1 cut(s) 715
BfuI GTATCC 1 cut(s) 991
BisI GCNGC 6 cut(s) 627, 630, 639, 687, 1323, 1577
BlnI CCTAGG 1 cut(s) 1232
BlsI GCNGC 6 cut(s) 628, 631, 640, 688, 1324, 1578
Bme1390I CCNGG 3 cut(s) 779, 908, 1419
BmeT110I CYCGRG 1 cut(s) 360
BmgBI CACGTC 1 cut(s) 471
BmgT120I GGNCC 2 cut(s) 703, 1334
BmiI GGNNCC 2 cut(s) 306, 1416
BmrFI CCNGG 3 cut(s) 779, 908, 1419
BmsI GCATC 3 cut(s) 424, 450, 485
BpiI GAAGAC 1 cut(s) 1380
BplI GAGNNNNNCTC 2 cut(s) 356, 388
BpmI CTGGAG 3 cut(s) 424, 779, 1440
BpuEI CTTGAG 3 cut(s) 1311, 1505, 1526
BpuMI CCSGG 1 cut(s) 908
Bsa29I ATCGAT 1 cut(s) 854
BsaJI CCNNGG 2 cut(s) 1232, 1393
BsaXI ACNNNNNCTCC 2 cut(s) 501, 531
Bsc4I CCNNNNNNNGG 6 cut(s) 58, 127, 215, 700, 1104, 1238
Bse1I ACTGG 7 cut(s) 23, 116, 180, 507, 762, 1098, 1345
Bse3DI GCAATG 2 cut(s) 633, 657
BseBI CCWGG 2 cut(s) 779, 1419
BseCI ATCGAT 1 cut(s) 854
BseDI CCNNGG 2 cut(s) 1232, 1393
BseGI GGATG 4 cut(s) 19, 176, 511, 1159
BseLI CCNNNNNNNGG 6 cut(s) 58, 127, 215, 700, 1104, 1238
BseMI GCAATG 2 cut(s) 633, 657
BseMII CTCAG 5 cut(s) 251, 729, 774, 966, 1470
BseNI ACTGG 7 cut(s) 23, 116, 180, 507, 762, 1098, 1345
BseRI GAGGAG 2 cut(s) 1207, 1258
BseXI GCAGC 5 cut(s) 616, 625, 673, 1309, 1563
BshFI GGCC 3 cut(s) 656, 704, 1336
BshVI ATCGAT 1 cut(s) 854
BsiHKAI GWGCWC 1 cut(s) 1512
BsiHKCI CYCGRG 1 cut(s) 360
BsiSI CCGG 1 cut(s) 908
BslI CCNNNNNNNGG 6 cut(s) 58, 127, 215, 700, 1104, 1238
BsmAI GTCTC 6 cut(s) 240, 258, 359, 1169, 1220, 1481
BsmI GAATGC 2 cut(s) 689, 1135
BsnI GGCC 3 cut(s) 656, 704, 1336
BsoBI CYCGRG 1 cut(s) 360
Bsp1286I GDGCHC 1 cut(s) 1512
Bsp1407I TGTACA 1 cut(s) 1047
Bsp143I GATC 5 cut(s) 547, 660, 772, 1414, 1492
BspACI CCGC 3 cut(s) 627, 1538, 1588
BspANI GGCC 3 cut(s) 656, 704, 1336
BspCNI CTCAG 5 cut(s) 250, 730, 775, 967, 1469
BspDI ATCGAT 1 cut(s) 854
BspHI TCATGA 1 cut(s) 1495
BspLI GGNNCC 2 cut(s) 306, 1416
BspPI GGATC 2 cut(s) 1409, 1422
BspQI GCTCTTC 2 cut(s) 723, 1061
BspTI CTTAAG 1 cut(s) 715
BsrBI CCGCTC 1 cut(s) 1538
BsrDI GCAATG 2 cut(s) 633, 657
BsrGI TGTACA 1 cut(s) 1047
BsrI ACTGG 7 cut(s) 23, 116, 180, 507, 762, 1098, 1345
BssECI CCNNGG 2 cut(s) 1232, 1393
BssMI GATC 5 cut(s) 547, 660, 772, 1414, 1492
BssT1I CCWWGG 1 cut(s) 1232
Bst2UI CCWGG 2 cut(s) 779, 1419
Bst4CI ACNGT 3 cut(s) 147, 400, 922
Bst6I CTCTTC 8 cut(s) 274, 358, 672, 723, 1061, 1218, 1263, 1530
BstAFI CTTAAG 1 cut(s) 715
BstAPI GCANNNNNTGC 1 cut(s) 1585
BstAUI TGTACA 1 cut(s) 1047
BstC8I GCNNGC 2 cut(s) 294, 735
BstDEI CTNAG 6 cut(s) 237, 738, 783, 831, 975, 1456
BstDSI CCRYGG 1 cut(s) 1393
BstENI CCTNNNNNAGG 1 cut(s) 1236
BstF5I GGATG 4 cut(s) 19, 176, 511, 1159
BstKTI GATC 5 cut(s) 550, 663, 775, 1417, 1495
BstMAI GTCTC 6 cut(s) 240, 258, 359, 1169, 1220, 1481
BstMBI GATC 5 cut(s) 547, 660, 772, 1414, 1492
BstMWI GCNNNNNNNGC 4 cut(s) 635, 638, 647, 1585
BstNI CCWGG 2 cut(s) 779, 1419
BstNSI RCATGY 1 cut(s) 1516
BstSCI CCNGG 3 cut(s) 777, 906, 1417
BstSFI CTRYAG 2 cut(s) 32, 189
BstV1I GCAGC 5 cut(s) 616, 625, 673, 1309, 1563
BstV2I GAAGAC 1 cut(s) 1380
BstX2I RGATCY 1 cut(s) 1414
BstYI RGATCY 1 cut(s) 1414
Bsu15I ATCGAT 1 cut(s) 854
BsuI GTATCC 1 cut(s) 991
BsuRI GGCC 3 cut(s) 656, 704, 1336
BsuTUI ATCGAT 1 cut(s) 854
BtgI CCRYGG 1 cut(s) 1393
BtrI CACGTC 1 cut(s) 471
BtsCI GGATG 4 cut(s) 19, 176, 511, 1159
BtsI GCAGTG 2 cut(s) 303, 709
BtsIMutI CAGTG 5 cut(s) 303, 500, 526, 709, 755
Cac8I GCNNGC 2 cut(s) 294, 735
CciI TCATGA 1 cut(s) 1495
Cfr13I GGNCC 2 cut(s) 703, 1334
ClaI ATCGAT 1 cut(s) 854
Csp6I GTAC 1 cut(s) 1048
CviAII CATG 7 cut(s) 58, 215, 658, 1283, 1411, 1496, 1513
CviQI GTAC 1 cut(s) 1048
DdeI CTNAG 6 cut(s) 237, 738, 783, 831, 975, 1456
DpnI GATC 5 cut(s) 549, 662, 774, 1416, 1494
DpnII GATC 5 cut(s) 547, 660, 772, 1414, 1492
EaeI YGGCCR 1 cut(s) 654
Eam1104I CTCTTC 8 cut(s) 274, 358, 672, 723, 1061, 1218, 1263, 1530
EarI CTCTTC 8 cut(s) 274, 358, 672, 723, 1061, 1218, 1263, 1530
Eco130I CCWWGG 1 cut(s) 1232
Eco32I GATATC 1 cut(s) 1408
Eco57I CTGAAG 1 cut(s) 120
Eco88I CYCGRG 1 cut(s) 360
EcoNI CCTNNNNNAGG 1 cut(s) 1236
EcoO109I RGGNCCY 1 cut(s) 703
EcoRII CCWGG 2 cut(s) 777, 1417
EcoRV GATATC 1 cut(s) 1408
EcoT14I CCWWGG 1 cut(s) 1232
EcoT22I ATGCAT 1 cut(s) 587
ErhI CCWWGG 1 cut(s) 1232
FaeI CATG 7 cut(s) 61, 218, 661, 1286, 1414, 1499, 1516
FalI AAGNNNNNCTT 4 cut(s) 253, 285, 824, 856
FatI CATG 7 cut(s) 57, 214, 657, 1282, 1410, 1495, 1512
FauNDI CATATG 3 cut(s) 696, 1149, 1302
FbaI TGATCA 3 cut(s) 547, 660, 1492
FblI GTMKAC 1 cut(s) 1198
Fnu4HI GCNGC 6 cut(s) 627, 630, 639, 687, 1323, 1577
FokI GGATG 4 cut(s) 6, 163, 518, 1166
Fsp4HI GCNGC 6 cut(s) 627, 630, 639, 687, 1323, 1577
FspBI CTAG 3 cut(s) 821, 1182, 1233
GluI GCNGC 6 cut(s) 627, 630, 639, 687, 1323, 1577
GsuI CTGGAG 3 cut(s) 424, 779, 1440
HaeIII GGCC 3 cut(s) 656, 704, 1336
HapII CCGG 1 cut(s) 908
Hin1II CATG 7 cut(s) 61, 218, 661, 1286, 1414, 1499, 1516
HindIII AAGCTT 2 cut(s) 102, 1082
HpaII CCGG 1 cut(s) 908
HphI GGTGA 3 cut(s) 428, 545, 1367
Hpy166II GTNNAC 1 cut(s) 1199
Hpy188I TCNGA 6 cut(s) 151, 244, 1012, 1090, 1459, 1610
Hpy188III TCNNGA 9 cut(s) 71, 362, 494, 1220, 1328, 1442, 1496, 1505, 1559
Hpy8I GTNNAC 1 cut(s) 1199
HpyAV CCTTC 6 cut(s) 295, 351, 524, 716, 781, 1535
HpyCH4III ACNGT 3 cut(s) 147, 400, 922
HpyCH4IV ACGT 2 cut(s) 470, 747
HpyCH4V TGCA 7 cut(s) 452, 585, 641, 689, 960, 1282, 1579
HpyF10VI GCNNNNNNNGC 4 cut(s) 635, 638, 647, 1585
HpyF3I CTNAG 6 cut(s) 237, 738, 783, 831, 975, 1456
HpySE526I ACGT 2 cut(s) 470, 747
Hsp92II CATG 7 cut(s) 61, 218, 661, 1286, 1414, 1499, 1516
Ksp22I TGATCA 3 cut(s) 547, 660, 1492
Kzo9I GATC 5 cut(s) 547, 660, 772, 1414, 1492
LguI GCTCTTC 2 cut(s) 723, 1061
LmnI GCTCC 2 cut(s) 675, 1535
Lsp1109I GCAGC 5 cut(s) 616, 625, 673, 1309, 1563
LweI GCATC 3 cut(s) 424, 450, 485
MaeI CTAG 3 cut(s) 821, 1182, 1233
MaeII ACGT 2 cut(s) 470, 747
MaeIII GTNAC 3 cut(s) 141, 952, 992
MalI GATC 5 cut(s) 549, 662, 774, 1416, 1494
MbiI CCGCTC 1 cut(s) 1538
MboI GATC 5 cut(s) 547, 660, 772, 1414, 1492
MflI RGATCY 1 cut(s) 1414
MhlI GDGCHC 1 cut(s) 1512
MlsI TGGCCA 1 cut(s) 656
MluCI AATT 7 cut(s) 525, 605, 824, 1043, 1057, 1361, 1550
MluNI TGGCCA 1 cut(s) 656
MlyI GAGTC 6 cut(s) 260, 344, 1171, 1204, 1222, 1255
MmeI TCCRAC 1 cut(s) 1455
Mox20I TGGCCA 1 cut(s) 656
Mph1103I ATGCAT 1 cut(s) 587
MscI TGGCCA 1 cut(s) 656
MseI TTAA 8 cut(s) 5, 162, 255, 554, 716, 1125, 1253, 1464
MslI CAYNNNNRTG 1 cut(s) 1152
Msp20I TGGCCA 1 cut(s) 656
MspA1I CMGCKG 2 cut(s) 629, 1576
MspCI CTTAAG 1 cut(s) 715
MspI CCGG 1 cut(s) 908
MspR9I CCNGG 3 cut(s) 779, 908, 1419
Mva1269I GAATGC 2 cut(s) 689, 1135
MvaI CCWGG 2 cut(s) 779, 1419
MwoI GCNNNNNNNGC 4 cut(s) 635, 638, 647, 1585
NciI CCSGG 1 cut(s) 908
NdeI CATATG 3 cut(s) 696, 1149, 1302
NdeII GATC 5 cut(s) 547, 660, 772, 1414, 1492
NlaIII CATG 7 cut(s) 61, 218, 661, 1286, 1414, 1499, 1516
NlaIV GGNNCC 2 cut(s) 306, 1416
NmuCI GTSAC 1 cut(s) 141
NsiI ATGCAT 1 cut(s) 587
NspI RCATGY 1 cut(s) 1516
PagI TCATGA 1 cut(s) 1495
PciSI GCTCTTC 2 cut(s) 723, 1061
PctI GAATGC 2 cut(s) 689, 1135
PfeI GAWTC 6 cut(s) 80, 485, 497, 851, 865, 1388
PflMI CCANNNNNTGG 2 cut(s) 58, 215
PfoI TCCNGGA 1 cut(s) 1417
PkrI GCNGC 6 cut(s) 628, 631, 640, 688, 1324, 1578
PleI GAGTC 6 cut(s) 260, 344, 1171, 1203, 1222, 1254
PpsI GAGTC 6 cut(s) 260, 344, 1171, 1203, 1222, 1254
PsiI TTATAA 1 cut(s) 110
Psp6I CCWGG 2 cut(s) 777, 1417
PspGI CCWGG 2 cut(s) 777, 1417
PspN4I GGNNCC 2 cut(s) 306, 1416
PspPI GGNCC 2 cut(s) 703, 1334
PsuI RGATCY 1 cut(s) 1414
PvuII CAGCTG 1 cut(s) 1576
RsaI GTAC 1 cut(s) 1049
RsaNI GTAC 1 cut(s) 1048
RseI CAYNNNNRTG 1 cut(s) 1152
SapI GCTCTTC 2 cut(s) 723, 1061
SaqAI TTAA 8 cut(s) 5, 162, 255, 554, 716, 1125, 1253, 1464
SatI GCNGC 6 cut(s) 627, 630, 639, 687, 1323, 1577
Sau3AI GATC 5 cut(s) 547, 660, 772, 1414, 1492
Sau96I GGNCC 2 cut(s) 703, 1334
SchI GAGTC 6 cut(s) 260, 344, 1171, 1204, 1222, 1255
ScrFI CCNGG 3 cut(s) 779, 908, 1419
SduI GDGCHC 1 cut(s) 1512
SfaNI GCATC 3 cut(s) 424, 450, 485
SfcI CTRYAG 2 cut(s) 32, 189
SmiMI CAYNNNNRTG 1 cut(s) 1152
SmlI CTYRAG 4 cut(s) 715, 1326, 1484, 1505
SmoI CTYRAG 4 cut(s) 715, 1326, 1484, 1505
Sse9I AATT 7 cut(s) 525, 605, 824, 1043, 1057, 1361, 1550
SsiI CCGC 3 cut(s) 627, 1538, 1588
SspMI CTAG 3 cut(s) 821, 1182, 1233
StyD4I CCNGG 3 cut(s) 777, 906, 1417
StyI CCWWGG 1 cut(s) 1232
TaaI ACNGT 3 cut(s) 147, 400, 922
TaiI ACGT 2 cut(s) 473, 750
TaqI TCGA 3 cut(s) 70, 854, 1558
TasI AATT 7 cut(s) 525, 605, 824, 1043, 1057, 1361, 1550
TatI WGTACW 1 cut(s) 1047
TauI GCSGC 1 cut(s) 629
TfiI GAWTC 6 cut(s) 80, 485, 497, 851, 865, 1388
Tru1I TTAA 8 cut(s) 5, 162, 255, 554, 716, 1125, 1253, 1464
Tru9I TTAA 8 cut(s) 5, 162, 255, 554, 716, 1125, 1253, 1464
TscAI CASTG 5 cut(s) 303, 507, 526, 716, 762
TseFI GTSAC 1 cut(s) 141
TseI GCWGC 5 cut(s) 629, 638, 686, 1322, 1576
Tsp45I GTSAC 1 cut(s) 141
TspDTI ATGAA 7 cut(s) 8, 72, 165, 864, 1053, 1374, 1464
TspRI CASTG 5 cut(s) 303, 507, 526, 716, 762
Van91I CCANNNNNTGG 2 cut(s) 58, 215
Vha464I CTTAAG 1 cut(s) 715
XagI CCTNNNNNAGG 1 cut(s) 1236
XapI RAATTY 1 cut(s) 824
XceI RCATGY 1 cut(s) 1516
XcmI CCANNNNNNNNNTGG 1 cut(s) 775
XmaJI CCTAGG 1 cut(s) 1232
XmiI GTMKAC 1 cut(s) 1198
XspI CTAG 3 cut(s) 821, 1182, 1233
Zsp2I ATGCAT 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.