Rmu_sc0000800.1_g000029

formamidase C869.04 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000800.1
OG0003336
Physical Location & Seq
Forward (+)
95510 .. 98618
3109 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1473 bp
atggctgtcaaacttcaatccttagatgaaagctgcatatcatcacttcaaccaaaacaaggaatcttcaatccatcatcttaccattgctcatcttcaacaagaccaaaaatggcttcttcttatggagcaagactggttgtgccgatagatgtgaaaaagaagccatgggagcagaaacttccacttcacaaccggtggcacccggatataccacctgtggcagaagctacggtgggagaggtttttcgagttgaaatggttgatttcagcgggggaggtatcacaaaggaatacactgccgaggacatcaagaatgccaatccttcaattgttcattatctcagcggaccgattaggatttcagacaaggatggaattccagcacagccaggtgatcttctggtagttgagatatgcaacttgggtcctcttccaggagatgaatggggtttcacagcaacattcgacagagaaaatggagggggatttctgactgaccattttccttgtgcaaccaaagctatttggtattttgaagggatatatgcctactctcctcaaataccaggagtgagattcccagggttaacacaccctggaatagttggaacagcaccatcaacggaactcttgaacatatggaataagagggagagagaacttgaggaaaatggagtcaagtctctgaaactatgtgaggttgtgcaccaacggcccttggctagcctaccatcaacaaaaggttgcgtccttggagggatcaaagagggcactcccgaatgggaaaagatagcagtggaggccgcaagaacaattccaggaagagaaaatggtggcaattgtgacatcaagaatcttagtagaggttcaaaggtataccttccagtattcattgaaggagcaaatcttagcactggtgacatgcacttttcacagggagatggcgaaatcgcattctgtggtgcgattgagatgagtggtttcctggagctcaagtgtgaaattataagggatggaatgaaagaatacctaacaccgatggggccaactcctcttcatgtgaatccaatctttgaaataggtcccgtcgagccaagattctcggaatggttggtatttgagggcattagtgttgatgagagtgggaagcagcactacctagatgcaactgttgcttacaagcgtgcagtactgaatgccatcgactacctctctaaatttggatactcaaaagaacagagctaccttctgctgtcatgctgtccatgcgagggaagaatttctggaatagtcgacgctcccaatgctgttgcaaccctagcaatcccaacagctatctttgaccaggatattcgaccaaaaacgaacaaggtcccggttggtccccggatagtgaggacaccagatgtcctaaaatgtacttacgatggaaatttgccaacgacaaggaaccctagctcagcaacataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00630 ko00910 ko01200 map00460 map00630 map00910 map01200
Pfam Domains
Protein Families
FmdA_AmdA

Protein Analysis

490

Amino Acids

53.55

Weight (kDa)

5.69

Isoelectric Point (pI)

40.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FmdA_AmdA PF03069 50 - 450 1.5e-164 Acetamidase/Formamidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1010
AccB1I GGYRCC 1 cut(s) 201
AccI GTMKAC 2 cut(s) 879, 1296
AciI CCGC 3 cut(s) 273, 348, 807
AclWI GGATC 1 cut(s) 770
AcsI RAATTY 4 cut(s) 378, 1220, 1281, 1435
AfaI GTAC 2 cut(s) 1194, 1423
AfiI CCNNNNNNNGG 3 cut(s) 59, 437, 1274
AgeI ACCGGT 1 cut(s) 195
AjnI CCWGG 8 cut(s) 391, 436, 568, 583, 598, 820, 987, 1347
AjuI GAANNNNNNNTTGG 2 cut(s) 100, 132
AluBI AGCT 7 cut(s) 33, 230, 524, 994, 1245, 1337, 1461
AluI AGCT 7 cut(s) 33, 230, 524, 994, 1245, 1337, 1461
Alw21I GWGCWC 2 cut(s) 711, 996
Alw26I GTCTC 1 cut(s) 690
Alw44I GTGCAC 1 cut(s) 707
AlwI GGATC 1 cut(s) 770
AoxI GGCC 3 cut(s) 716, 804, 1046
ApaLI GTGCAC 1 cut(s) 707
ApeKI GCWGC 2 cut(s) 33, 1153
ApoI RAATTY 4 cut(s) 378, 1220, 1281, 1435
AsiGI ACCGGT 1 cut(s) 195
Asp700I GAANNNNTTC 1 cut(s) 1282
AspS9I GGNCC 7 cut(s) 350, 428, 717, 1046, 1085, 1375, 1385
AsuC2I CCSGG 3 cut(s) 206, 1379, 1390
AsuHPI GGTGA 2 cut(s) 407, 932
AsuNHI GCTAGC 1 cut(s) 725
AvaII GGWCC 5 cut(s) 350, 428, 1085, 1375, 1385
BaeGI GKGCMC 2 cut(s) 711, 776
BanI GGYRCC 1 cut(s) 201
BanII GRGCYC 1 cut(s) 996
BarI GAAGNNNNNNTAC 2 cut(s) 1142, 1174
Bbv12I GWGCWC 2 cut(s) 711, 996
BbvI GCAGC 2 cut(s) 20, 1165
BccI CCATC 9 cut(s) 82, 368, 628, 742, 938, 1010, 1036, 1211, 1424
BceAI ACGGC 1 cut(s) 731
BciT130I CCWGG 8 cut(s) 393, 438, 570, 585, 600, 822, 989, 1349
BciVI GTATCC 1 cut(s) 1220
BcnI CCSGG 3 cut(s) 206, 1379, 1390
BcoDI GTCTC 1 cut(s) 690
BfaI CTAG 4 cut(s) 726, 1163, 1322, 1458
BfuI GTATCC 1 cut(s) 1220
BisI GCNGC 3 cut(s) 34, 807, 1154
BlpI GCTNAGC 1 cut(s) 1462
BlsI GCNGC 3 cut(s) 35, 808, 1155
BmcAI AGTACT 1 cut(s) 1194
Bme18I GGWCC 5 cut(s) 350, 428, 1085, 1375, 1385
BmgT120I GGNCC 7 cut(s) 350, 428, 717, 1046, 1085, 1375, 1385
BmiI GGNNCC 7 cut(s) 203, 429, 1047, 1087, 1377, 1387, 1454
BmsI GCATC 1 cut(s) 1156
BmtI GCTAGC 1 cut(s) 729
BpmI CTGGAG 1 cut(s) 1010
Bpu1102I GCTNAGC 1 cut(s) 1462
BpuEI CTTGAG 2 cut(s) 686, 980
BpuMI CCSGG 3 cut(s) 206, 1379, 1390
BsaJI CCNNGG 8 cut(s) 167, 303, 583, 584, 598, 720, 754, 1388
BsaWI WCCGGW 1 cut(s) 195
BsaXI ACNNNNNCTCC 2 cut(s) 983, 1013
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 437, 1274
Bse118I RCCGGY 1 cut(s) 195
Bse1I ACTGG 3 cut(s) 141, 887, 922
Bse3DI GCAATG 1 cut(s) 85
BseBI CCWGG 8 cut(s) 393, 438, 570, 585, 600, 822, 989, 1349
BseDI CCNNGG 8 cut(s) 167, 303, 583, 584, 598, 720, 754, 1388
BseGI GGATG 2 cut(s) 379, 1021
BseLI CCNNNNNNNGG 3 cut(s) 59, 437, 1274
BseMI GCAATG 1 cut(s) 85
BseMII CTCAG 1 cut(s) 358
BseNI ACTGG 3 cut(s) 141, 887, 922
BseRI GAGGAG 2 cut(s) 549, 1044
BseSI GKGCMC 2 cut(s) 711, 776
BseXI GCAGC 2 cut(s) 20, 1165
BsgI GTGCAG 1 cut(s) 1209
BshFI GGCC 3 cut(s) 718, 806, 1048
BshNI GGYRCC 1 cut(s) 201
BshTI ACCGGT 1 cut(s) 195
BsiHKAI GWGCWC 2 cut(s) 711, 996
BsiSI CCGG 4 cut(s) 196, 206, 1379, 1390
BslFI GGGAC 3 cut(s) 1071, 1361, 1371
BslI CCNNNNNNNGG 3 cut(s) 59, 437, 1274
BsmAI GTCTC 1 cut(s) 690
BsmFI GGGAC 3 cut(s) 1071, 1361, 1371
BsmI GAATGC 3 cut(s) 322, 956, 1204
BsnI GGCC 3 cut(s) 718, 806, 1048
Bsp1286I GDGCHC 3 cut(s) 711, 776, 996
Bsp143I GATC 2 cut(s) 397, 762
Bsp1720I GCTNAGC 1 cut(s) 1462
Bsp19I CCATGG 1 cut(s) 167
BspACI CCGC 3 cut(s) 273, 348, 807
BspANI GGCC 3 cut(s) 718, 806, 1048
BspCNI CTCAG 1 cut(s) 357
BspLI GGNNCC 7 cut(s) 203, 429, 1047, 1087, 1377, 1387, 1454
BspOI GCTAGC 1 cut(s) 729
BspPI GGATC 1 cut(s) 770
BspT107I GGYRCC 1 cut(s) 201
BsrDI GCAATG 1 cut(s) 85
BsrFI RCCGGY 1 cut(s) 195
BsrI ACTGG 3 cut(s) 141, 887, 922
BssAI RCCGGY 1 cut(s) 195
BssECI CCNNGG 8 cut(s) 167, 303, 583, 584, 598, 720, 754, 1388
BssMI GATC 2 cut(s) 397, 762
BssNAI GTATAC 1 cut(s) 880
BssT1I CCWWGG 3 cut(s) 167, 720, 754
Bst1107I GTATAC 1 cut(s) 880
Bst2UI CCWGG 8 cut(s) 393, 438, 570, 585, 600, 822, 989, 1349
Bst4CI ACNGT 2 cut(s) 235, 1174
Bst6I CTCTTC 3 cut(s) 438, 820, 1062
BstAPI GCANNNNNTGC 1 cut(s) 1175
BstC8I GCNNGC 2 cut(s) 727, 1188
BstDEI CTNAG 5 cut(s) 22, 344, 860, 911, 1462
BstDSI CCRYGG 1 cut(s) 167
BstENI CCTNNNNNAGG 1 cut(s) 435
BstF5I GGATG 2 cut(s) 379, 1021
BstKTI GATC 2 cut(s) 400, 765
BstMAI GTCTC 1 cut(s) 690
BstMBI GATC 2 cut(s) 397, 762
BstMWI GCNNNNNNNGC 8 cut(s) 172, 521, 715, 803, 1175, 1269, 1307, 1322
BstNI CCWGG 8 cut(s) 393, 438, 570, 585, 600, 822, 989, 1349
BstNSI RCATGY 1 cut(s) 928
BstSLI GKGCMC 2 cut(s) 711, 776
BstV1I GCAGC 2 cut(s) 20, 1165
BstZ17I GTATAC 1 cut(s) 880
BsuI GTATCC 1 cut(s) 1220
BsuRI GGCC 3 cut(s) 718, 806, 1048
BtgI CCRYGG 1 cut(s) 167
BtsCI GGATG 2 cut(s) 379, 1021
BtsI GCAGTG 2 cut(s) 297, 804
BtsIMutI CAGTG 3 cut(s) 297, 804, 915
Cac8I GCNNGC 2 cut(s) 727, 1188
Cfr10I RCCGGY 1 cut(s) 195
Cfr13I GGNCC 7 cut(s) 350, 428, 717, 1046, 1085, 1375, 1385
CpoI CGGWCCG 1 cut(s) 350
CseI GACGC 2 cut(s) 739, 1307
Csp6I GTAC 2 cut(s) 1193, 1422
CspAI ACCGGT 1 cut(s) 195
CspI CGGWCCG 1 cut(s) 350
CviAII CATG 5 cut(s) 168, 925, 1061, 1260, 1269
CviQI GTAC 2 cut(s) 1193, 1422
DdeI CTNAG 5 cut(s) 22, 344, 860, 911, 1462
DpnI GATC 2 cut(s) 399, 764
DpnII GATC 2 cut(s) 397, 762
Eam1104I CTCTTC 3 cut(s) 438, 820, 1062
EarI CTCTTC 3 cut(s) 438, 820, 1062
Ecl136II GAGCTC 1 cut(s) 994
Eco130I CCWWGG 3 cut(s) 167, 720, 754
Eco24I GRGCYC 1 cut(s) 996
Eco47I GGWCC 5 cut(s) 350, 428, 1085, 1375, 1385
Eco53kI GAGCTC 1 cut(s) 994
EcoICRI GAGCTC 1 cut(s) 994
EcoNI CCTNNNNNAGG 1 cut(s) 435
EcoO109I RGGNCCY 3 cut(s) 428, 1085, 1375
EcoRI GAATTC 1 cut(s) 378
EcoRII CCWGG 8 cut(s) 391, 436, 568, 583, 598, 820, 987, 1347
EcoT14I CCWWGG 3 cut(s) 167, 720, 754
EcoT38I GRGCYC 1 cut(s) 996
ErhI CCWWGG 3 cut(s) 167, 720, 754
FaeI CATG 5 cut(s) 171, 928, 1064, 1263, 1272
FaqI GGGAC 3 cut(s) 1071, 1361, 1371
FatI CATG 5 cut(s) 167, 924, 1060, 1259, 1268
FauI CCCGC 1 cut(s) 266
FauNDI CATATG 1 cut(s) 641
FblI GTMKAC 2 cut(s) 879, 1296
Fnu4HI GCNGC 3 cut(s) 34, 807, 1154
FokI GGATG 2 cut(s) 386, 1028
FriOI GRGCYC 1 cut(s) 996
Fsp4HI GCNGC 3 cut(s) 34, 807, 1154
FspBI CTAG 4 cut(s) 726, 1163, 1322, 1458
GluI GCNGC 3 cut(s) 34, 807, 1154
GsuI CTGGAG 1 cut(s) 1010
HaeIII GGCC 3 cut(s) 718, 806, 1048
HapII CCGG 4 cut(s) 196, 206, 1379, 1390
HgaI GACGC 2 cut(s) 739, 1307
Hin1II CATG 5 cut(s) 171, 928, 1064, 1263, 1272
HincII GTYRAC 2 cut(s) 591, 1297
HindII GTYRAC 2 cut(s) 591, 1297
HinfI GANTC 6 cut(s) 63, 579, 678, 856, 1066, 1101
HpaI GTTAAC 1 cut(s) 591
HpaII CCGG 4 cut(s) 196, 206, 1379, 1390
HphI GGTGA 2 cut(s) 407, 932
Hpy166II GTNNAC 4 cut(s) 591, 709, 880, 1297
Hpy188I TCNGA 4 cut(s) 367, 495, 690, 1108
Hpy188III TCNNGA 5 cut(s) 313, 634, 779, 853, 1287
Hpy8I GTNNAC 4 cut(s) 591, 709, 880, 1297
Hpy99I CGWCG 2 cut(s) 1094, 1301
HpyAV CCTTC 5 cut(s) 336, 533, 893, 893, 1259
HpyCH4III ACNGT 2 cut(s) 235, 1174
HpyCH4V TGCA 8 cut(s) 36, 420, 515, 709, 928, 1169, 1190, 1316
HpyF10VI GCNNNNNNNGC 8 cut(s) 172, 521, 715, 803, 1175, 1269, 1307, 1322
HpyF3I CTNAG 5 cut(s) 22, 344, 860, 911, 1462
Hsp92II CATG 5 cut(s) 171, 928, 1064, 1263, 1272
KspAI GTTAAC 1 cut(s) 591
Kzo9I GATC 2 cut(s) 397, 762
LmnI GCTCC 5 cut(s) 128, 172, 902, 991, 1306
Lsp1109I GCAGC 2 cut(s) 20, 1165
LweI GCATC 1 cut(s) 1156
MaeI CTAG 4 cut(s) 726, 1163, 1322, 1458
MaeIII GTNAC 2 cut(s) 845, 920
MalI GATC 2 cut(s) 399, 764
MboI GATC 2 cut(s) 397, 762
MboII GAAGA 8 cut(s) 58, 87, 111, 392, 425, 837, 1049, 1290
MfeI CAATTG 2 cut(s) 330, 841
MhlI GDGCHC 3 cut(s) 711, 776, 996
MluCI AATT 8 cut(s) 330, 378, 816, 841, 1005, 1220, 1281, 1435
MlyI GAGTC 1 cut(s) 687
MmeI TCCRAC 1 cut(s) 589
MroXI GAANNNNTTC 1 cut(s) 1282
MseI TTAA 1 cut(s) 590
MspA1I CMGCKG 2 cut(s) 273, 348
MspI CCGG 4 cut(s) 196, 206, 1379, 1390
MunI CAATTG 2 cut(s) 330, 841
Mva1269I GAATGC 3 cut(s) 322, 956, 1204
MvaI CCWGG 8 cut(s) 393, 438, 570, 585, 600, 822, 989, 1349
MwoI GCNNNNNNNGC 8 cut(s) 172, 521, 715, 803, 1175, 1269, 1307, 1322
NciI CCSGG 3 cut(s) 206, 1379, 1390
NcoI CCATGG 1 cut(s) 167
NdeI CATATG 1 cut(s) 641
NdeII GATC 2 cut(s) 397, 762
NheI GCTAGC 1 cut(s) 725
NlaIII CATG 5 cut(s) 171, 928, 1064, 1263, 1272
NlaIV GGNNCC 7 cut(s) 203, 429, 1047, 1087, 1377, 1387, 1454
NmeAIII GCCGAG 1 cut(s) 328
NmuCI GTSAC 2 cut(s) 845, 920
NspI RCATGY 1 cut(s) 928
PasI CCCWGGG 1 cut(s) 584
PctI GAATGC 3 cut(s) 322, 956, 1204
PdmI GAANNNNTTC 1 cut(s) 1282
PfeI GAWTC 5 cut(s) 63, 579, 856, 1066, 1101
PfoI TCCNGGA 3 cut(s) 436, 820, 987
PinAI ACCGGT 1 cut(s) 195
PkrI GCNGC 3 cut(s) 35, 808, 1155
PleI GAGTC 1 cut(s) 686
PpsI GAGTC 1 cut(s) 686
PpuMI RGGWCCY 3 cut(s) 428, 1085, 1375
PsiI TTATAA 1 cut(s) 1010
Psp124BI GAGCTC 1 cut(s) 996
Psp5II RGGWCCY 3 cut(s) 428, 1085, 1375
Psp6I CCWGG 8 cut(s) 391, 436, 568, 583, 598, 820, 987, 1347
PspGI CCWGG 8 cut(s) 391, 436, 568, 583, 598, 820, 987, 1347
PspN4I GGNNCC 7 cut(s) 203, 429, 1047, 1087, 1377, 1387, 1454
PspPI GGNCC 7 cut(s) 350, 428, 717, 1046, 1085, 1375, 1385
PspPPI RGGWCCY 3 cut(s) 428, 1085, 1375
RsaI GTAC 2 cut(s) 1194, 1423
RsaNI GTAC 2 cut(s) 1193, 1422
Rsr2I CGGWCCG 1 cut(s) 350
RsrII CGGWCCG 1 cut(s) 350
SacI GAGCTC 1 cut(s) 996
SalI GTCGAC 1 cut(s) 1295
SaqAI TTAA 1 cut(s) 590
SatI GCNGC 3 cut(s) 34, 807, 1154
Sau3AI GATC 2 cut(s) 397, 762
Sau96I GGNCC 7 cut(s) 350, 428, 717, 1046, 1085, 1375, 1385
ScaI AGTACT 1 cut(s) 1194
SchI GAGTC 1 cut(s) 687
SduI GDGCHC 3 cut(s) 711, 776, 996
SfaNI GCATC 1 cut(s) 1156
SinI GGWCC 5 cut(s) 350, 428, 1085, 1375, 1385
SmlI CTYRAG 2 cut(s) 665, 995
SmoI CTYRAG 2 cut(s) 665, 995
Sse9I AATT 8 cut(s) 330, 378, 816, 841, 1005, 1220, 1281, 1435
SsiI CCGC 3 cut(s) 273, 348, 807
SspMI CTAG 4 cut(s) 726, 1163, 1322, 1458
SstI GAGCTC 1 cut(s) 996
StyI CCWWGG 3 cut(s) 167, 720, 754
TaaI ACNGT 2 cut(s) 235, 1174
TaqI TCGA 6 cut(s) 250, 468, 1092, 1206, 1296, 1357
TaqII GACCGA 1 cut(s) 367
TasI AATT 8 cut(s) 330, 378, 816, 841, 1005, 1220, 1281, 1435
TatI WGTACW 2 cut(s) 1192, 1421
TauI GCSGC 1 cut(s) 809
TfiI GAWTC 5 cut(s) 63, 579, 856, 1066, 1101
Tru1I TTAA 1 cut(s) 590
Tru9I TTAA 1 cut(s) 590
TscAI CASTG 3 cut(s) 304, 804, 922
TseFI GTSAC 2 cut(s) 845, 920
TseI GCWGC 2 cut(s) 33, 1153
Tsp45I GTSAC 2 cut(s) 845, 920
TspDTI ATGAA 6 cut(s) 42, 326, 459, 883, 1037, 1049
TspGWI ACGGA 1 cut(s) 641
TspRI CASTG 3 cut(s) 304, 804, 922
VneI GTGCAC 1 cut(s) 707
VpaK11BI GGWCC 5 cut(s) 350, 428, 1085, 1375, 1385
XagI CCTNNNNNAGG 1 cut(s) 435
XapI RAATTY 4 cut(s) 378, 1220, 1281, 1435
XceI RCATGY 1 cut(s) 928
XcmI CCANNNNNNNNNTGG 1 cut(s) 444
XmiI GTMKAC 2 cut(s) 879, 1296
XmnI GAANNNNTTC 1 cut(s) 1282
XspI CTAG 4 cut(s) 726, 1163, 1322, 1458
ZrmI AGTACT 1 cut(s) 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.