Rmu_sc0000898.1_g000092

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000898.1
OG0014867
Physical Location & Seq
Forward (+)
423498 .. 424082
585 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 585 bp
atgactattatactttctctcccccccccctctctctctctctctctaccccaagcggaggcaatccaatccctctctgccatcctcctctccctcatctacggcctctgccgccgctcgatcgacgcagccgtctgcatccccaagatcttccgatctgagtcggcgcttgcccagacggaattggctccagtgctgttcgaggagctcttcctcatccacctcctcctggttctccaatggtacaatcaccggcgatcggagattctgccatcgctgtccccaaatgtcagctatgaaaacgacgacgtttcaatcaacgacatgtcggtggcttttgcggcggggtcgaattcgctgtcgaagatgagtgggaaccagatggcggggttgaaggagatggacagcagctacgagagggttttggatgagaattgtagggttttcagtgagtatttcagggaggttttggagaataaagatggaggaaaagtgattgatccaccggccgtggtgttggaattgagtgaaggaagtgatgagttccggtatgatgatcggagcaccgaagaagatgaagcgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box WD40

Protein Analysis

194

Amino Acids

21.58

Weight (kDa)

4.39

Isoelectric Point (pI)

65.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 24 - 161 1.7e-14 E3 ubiquitin-protein ligase LIN-like, ARM repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 131
AccBSI CCGCTC 1 cut(s) 117
AciI CCGC 6 cut(s) 56, 112, 115, 341, 344, 386
AclWI GGATC 1 cut(s) 494
AcoI YGGCCR 1 cut(s) 507
AcsI RAATTY 1 cut(s) 352
AfaI GTAC 1 cut(s) 245
AfiI CCNNNNNNNGG 4 cut(s) 58, 229, 259, 385
AflIII ACRYGT 1 cut(s) 324
AgsI TTSAA 2 cut(s) 315, 394
AjnI CCWGG 1 cut(s) 228
AluBI AGCT 3 cut(s) 208, 294, 411
AluI AGCT 3 cut(s) 208, 294, 411
Alw21I GWGCWC 2 cut(s) 210, 566
AlwI GGATC 1 cut(s) 494
AoxI GGCC 2 cut(s) 103, 507
ApeKI GCWGC 2 cut(s) 128, 408
ApoI RAATTY 1 cut(s) 352
ArsI GACNNNNNNTTYG 2 cut(s) 344, 376
AspLEI GCGC 1 cut(s) 169
AsuHPI GGTGA 1 cut(s) 242
BanII GRGCYC 1 cut(s) 210
Bbv12I GWGCWC 2 cut(s) 210, 566
BbvI GCAGC 2 cut(s) 140, 420
BccI CCATC 5 cut(s) 89, 280, 376, 394, 476
BceAI ACGGC 3 cut(s) 116, 118, 494
BciT130I CCWGG 1 cut(s) 230
BfoI RGCGCY 1 cut(s) 170
BglII AGATCT 1 cut(s) 147
BisI GCNGC 5 cut(s) 112, 115, 129, 342, 409
BlsI GCNGC 5 cut(s) 113, 116, 130, 343, 410
Bme1390I CCNGG 1 cut(s) 230
BmiI GGNNCC 2 cut(s) 189, 377
BmrFI CCNGG 1 cut(s) 230
BmsI GCATC 1 cut(s) 147
BpmI CTGGAG 1 cut(s) 174
BsaJI CCNNGG 1 cut(s) 510
BsaWI WCCGGW 1 cut(s) 546
BsaXI ACNNNNNCTCC 3 cut(s) 33, 477, 507
Bsc4I CCNNNNNNNGG 4 cut(s) 58, 229, 259, 385
Bse118I RCCGGY 2 cut(s) 252, 505
Bse1I ACTGG 1 cut(s) 191
BseBI CCWGG 1 cut(s) 230
BseDI CCNNGG 1 cut(s) 510
BseGI GGATG 4 cut(s) 81, 138, 216, 433
BseLI CCNNNNNNNGG 4 cut(s) 58, 229, 259, 385
BseMII CTCAG 1 cut(s) 150
BseNI ACTGG 1 cut(s) 191
BseRI GAGGAG 3 cut(s) 77, 215, 218
BseX3I CGGCCG 1 cut(s) 507
BseXI GCAGC 2 cut(s) 140, 420
Bsh1285I CGRYCG 3 cut(s) 123, 260, 510
BshFI GGCC 2 cut(s) 105, 509
BsiEI CGRYCG 3 cut(s) 123, 260, 510
BsiHKAI GWGCWC 2 cut(s) 210, 566
BsiSI CCGG 3 cut(s) 253, 506, 547
BslFI GGGAC 1 cut(s) 265
BslI CCNNNNNNNGG 4 cut(s) 58, 229, 259, 385
BsmFI GGGAC 1 cut(s) 265
BsnI GGCC 2 cut(s) 105, 509
Bsp1286I GDGCHC 2 cut(s) 210, 566
Bsp143I GATC 6 cut(s) 120, 147, 155, 257, 499, 556
BspACI CCGC 6 cut(s) 56, 112, 115, 341, 344, 386
BspANI GGCC 2 cut(s) 105, 509
BspCNI CTCAG 1 cut(s) 151
BspLI GGNNCC 2 cut(s) 189, 377
BspPI GGATC 1 cut(s) 494
BspQI GCTCTTC 1 cut(s) 215
BsrBI CCGCTC 1 cut(s) 117
BsrFI RCCGGY 2 cut(s) 252, 505
BsrI ACTGG 1 cut(s) 191
BssAI RCCGGY 2 cut(s) 252, 505
BssECI CCNNGG 1 cut(s) 510
BssMI GATC 6 cut(s) 120, 147, 155, 257, 499, 556
Bst2UI CCWGG 1 cut(s) 230
Bst6I CTCTTC 1 cut(s) 215
BstC8I GCNNGC 1 cut(s) 171
BstDEI CTNAG 1 cut(s) 159
BstDSI CCRYGG 1 cut(s) 510
BstF5I GGATG 4 cut(s) 81, 138, 216, 433
BstH2I RGCGCY 1 cut(s) 170
BstHHI GCGC 1 cut(s) 169
BstKTI GATC 6 cut(s) 123, 150, 158, 260, 502, 559
BstMBI GATC 6 cut(s) 120, 147, 155, 257, 499, 556
BstMCI CGRYCG 3 cut(s) 123, 260, 510
BstMWI GCNNNNNNNGC 2 cut(s) 111, 341
BstNI CCWGG 1 cut(s) 230
BstNSI RCATGY 1 cut(s) 328
BstSCI CCNGG 1 cut(s) 228
BstV1I GCAGC 2 cut(s) 140, 420
BstX2I RGATCY 1 cut(s) 147
BstYI RGATCY 1 cut(s) 147
BstZI CGGCCG 1 cut(s) 507
BsuRI GGCC 2 cut(s) 105, 509
BtgI CCRYGG 1 cut(s) 510
BtgZI GCGATG 1 cut(s) 258
BtsCI GGATG 4 cut(s) 81, 138, 216, 433
BtsIMutI CAGTG 2 cut(s) 198, 454
Cac8I GCNNGC 1 cut(s) 171
CfoI GCGC 1 cut(s) 169
Cfr10I RCCGGY 2 cut(s) 252, 505
CseI GACGC 1 cut(s) 134
Csp6I GTAC 1 cut(s) 244
CviAII CATG 1 cut(s) 325
CviJI RGCY 8 cut(s) 105, 131, 188, 208, 294, 335, 411, 509
CviKI_1 RGCY 8 cut(s) 105, 131, 188, 208, 294, 335, 411, 509
CviQI GTAC 1 cut(s) 244
DdeI CTNAG 1 cut(s) 159
DpnI GATC 6 cut(s) 122, 149, 157, 259, 501, 558
DpnII GATC 6 cut(s) 120, 147, 155, 257, 499, 556
DrdI GACNNNNNNGTC 1 cut(s) 131
DseDI GACNNNNNNGTC 1 cut(s) 131
EaeI YGGCCR 1 cut(s) 507
EagI CGGCCG 1 cut(s) 507
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
Ecl136II GAGCTC 1 cut(s) 208
EclXI CGGCCG 1 cut(s) 507
Eco24I GRGCYC 1 cut(s) 210
Eco52I CGGCCG 1 cut(s) 507
Eco53kI GAGCTC 1 cut(s) 208
EcoICRI GAGCTC 1 cut(s) 208
EcoRI GAATTC 1 cut(s) 352
EcoRII CCWGG 1 cut(s) 228
EcoT38I GRGCYC 1 cut(s) 210
FaeI CATG 1 cut(s) 328
FaiI YATR 4 cut(s) 11, 297, 326, 552
FaqI GGGAC 1 cut(s) 265
FatI CATG 1 cut(s) 324
FauI CCCGC 2 cut(s) 337, 379
Fnu4HI GCNGC 5 cut(s) 112, 115, 129, 342, 409
FokI GGATG 4 cut(s) 68, 125, 203, 440
FriOI GRGCYC 1 cut(s) 210
Fsp4HI GCNGC 5 cut(s) 112, 115, 129, 342, 409
GlaI GCGC 1 cut(s) 168
GluI GCNGC 5 cut(s) 112, 115, 129, 342, 409
GsuI CTGGAG 1 cut(s) 174
HaeII RGCGCY 1 cut(s) 170
HaeIII GGCC 2 cut(s) 105, 509
HapII CCGG 3 cut(s) 253, 506, 547
HgaI GACGC 1 cut(s) 134
HhaI GCGC 1 cut(s) 169
Hin1II CATG 1 cut(s) 328
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HinfI GANTC 2 cut(s) 161, 265
HpaII CCGG 3 cut(s) 253, 506, 547
HphI GGTGA 1 cut(s) 242
Hpy188I TCNGA 4 cut(s) 155, 160, 262, 561
Hpy99I CGWCG 3 cut(s) 128, 308, 311
HpyAV CCTTC 2 cut(s) 388, 524
HpyCH4IV ACGT 1 cut(s) 309
HpyCH4V TGCA 1 cut(s) 138
HpyF10VI GCNNNNNNNGC 2 cut(s) 111, 341
HpyF3I CTNAG 1 cut(s) 159
HpySE526I ACGT 1 cut(s) 309
Hsp92II CATG 1 cut(s) 328
HspAI GCGC 1 cut(s) 167
Kzo9I GATC 6 cut(s) 120, 147, 155, 257, 499, 556
LguI GCTCTTC 1 cut(s) 215
LmnI GCTCC 3 cut(s) 193, 205, 561
LpnPI CCDG 9 cut(s) 188, 204, 215, 242, 266, 392, 445, 519, 560
Lsp1109I GCAGC 2 cut(s) 140, 420
LweI GCATC 1 cut(s) 147
MaeII ACGT 1 cut(s) 309
MalI GATC 6 cut(s) 122, 149, 157, 259, 501, 558
MbiI CCGCTC 1 cut(s) 117
MboI GATC 6 cut(s) 120, 147, 155, 257, 499, 556
MboII GAAGA 5 cut(s) 142, 202, 376, 581, 584
MflI RGATCY 1 cut(s) 147
MhlI GDGCHC 2 cut(s) 210, 566
MluCI AATT 4 cut(s) 182, 352, 433, 521
MlyI GAGTC 1 cut(s) 170
MmeI TCCRAC 1 cut(s) 498
MslI CAYNNNNRTG 1 cut(s) 329
MspI CCGG 3 cut(s) 253, 506, 547
MspR9I CCNGG 1 cut(s) 230
MvaI CCWGG 1 cut(s) 230
MwoI GCNNNNNNNGC 2 cut(s) 111, 341
NdeII GATC 6 cut(s) 120, 147, 155, 257, 499, 556
NlaIII CATG 1 cut(s) 328
NlaIV GGNNCC 2 cut(s) 189, 377
NspI RCATGY 1 cut(s) 328
PciI ACATGT 1 cut(s) 324
PciSI GCTCTTC 1 cut(s) 215
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PfeI GAWTC 1 cut(s) 265
PkrI GCNGC 5 cut(s) 113, 116, 130, 343, 410
Ple19I CGATCG 2 cut(s) 123, 260
PleI GAGTC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 169
PscI ACATGT 1 cut(s) 324
Psp124BI GAGCTC 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 228
PspGI CCWGG 1 cut(s) 228
PspN4I GGNNCC 2 cut(s) 189, 377
PsuI RGATCY 1 cut(s) 147
PvuI CGATCG 2 cut(s) 123, 260
RsaI GTAC 1 cut(s) 245
RsaNI GTAC 1 cut(s) 244
RseI CAYNNNNRTG 1 cut(s) 329
SacI GAGCTC 1 cut(s) 210
SapI GCTCTTC 1 cut(s) 215
SatI GCNGC 5 cut(s) 112, 115, 129, 342, 409
Sau3AI GATC 6 cut(s) 120, 147, 155, 257, 499, 556
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 1 cut(s) 230
SduI GDGCHC 2 cut(s) 210, 566
SetI ASST 6 cut(s) 210, 225, 296, 312, 413, 468
SfaNI GCATC 1 cut(s) 147
SgrAI CRCCGGYG 1 cut(s) 252
SmiMI CAYNNNNRTG 1 cut(s) 329
Sse9I AATT 4 cut(s) 182, 352, 433, 521
SsiI CCGC 6 cut(s) 56, 112, 115, 341, 344, 386
SstI GAGCTC 1 cut(s) 210
StyD4I CCNGG 1 cut(s) 228
TaiI ACGT 1 cut(s) 312
TaqI TCGA 5 cut(s) 119, 123, 201, 350, 362
TasI AATT 4 cut(s) 182, 352, 433, 521
TauI GCSGC 3 cut(s) 114, 117, 344
TfiI GAWTC 1 cut(s) 265
TscAI CASTG 2 cut(s) 198, 454
TseI GCWGC 2 cut(s) 128, 408
TspDTI ATGAA 1 cut(s) 312
TspGWI ACGGA 1 cut(s) 194
TspRI CASTG 2 cut(s) 198, 454
XapI RAATTY 1 cut(s) 352
XceI RCATGY 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.