Rroxscaffold_3G00261050

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014867
Physical Location & Seq
Forward (+)
55432555 .. 55440075
7521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00261050.1

Sequence Viewer

Length: 1077 bp
ATGGTGACGCGACGTTCTGGCTCAGAAGTGGGACGTGCAGGCTTGGGGCTCCAAGGGTGGAACGACCTCGGTTGGGGCTATAAGACTGGTGGTCTGGGTGGTGGCACTTGGAGTCATGTGGACGAAATAGGAACCTGTATTTTCCTGCGTTGCAGATCTGGCAGGGTGGTCGGATGGAGGGCTGAAGCCAAGGTGCCCAAGTGCTTGGGTGCCCAGATCAGATTGAGTTGGGCTTGGGCATTTGTCCAGGTTGACCCGTCAGCCTTGATTTTGGATTCGGGCCGGATTTGGATCCGCATTTGGGATCCGGTGAAATGCCGCCGGACGACTACCGAAGCTCGTGATTTCAACAGCCTTTGGCTCCGAGGGATCTTGACCGGGGTGTTCAATTGGCTTGGGATAAGCCTCGACGGTGGTTTTTGTGAGCACAATGGTCTTTTTTGTGGATCGATCCCGCCAAAAGTTCCAAGGTTCGATTCGGTGCATCTCTTTTTCTATAAGAACCGGATGGTAGGCATGGAAAGGCTTGGGGAGTCGTTCAAAAGGAAGGCAATCCAACCCCTCCCCGCCATCCTCCTCTCCCTCATCTACGACCTCTACCGCCGCTCAATCAACACCGCCGTCCGCATCCCCGAGATCTTCCGACCGAGCCGGCGCTCGCCGGACGGAATTGGCTCATGTGTCGTTCTAGAGCTCTTCCTCATCTACCTCCTCCGGTTCTCCGGTGGTACAATCGCCGGCGATCACAGATTCTCGCCGCCGCTGTCCCCAAATGTCGGCCTACCGAAACGACGACGTTTTGATCAACGACATGTCGGTGGCTTTTCCGGAGGGGTCGAATTCGCTGTCGAAGATAAGTGGGAACCGTACGGCGGGGTGAAGGAGATGGAGAGCAGCTATGAGAGGGTTTTGGATGAGAATTGTAGGGTTTTCAATGAGTATTTCGGGGAGGTTTTGGAGAATAAAGATGGAGGAAGAGTGATTGATCCATCGGCTGTGGTGTTGGACACTTCTACAAAAAATGCATCACACAACACTCATCACACAACAGAACAAAAATATGCTGTTGTGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box WD40

Protein Analysis

358

Amino Acids

40.17

Weight (kDa)

9.23

Isoelectric Point (pI)

49.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 193, 209
AccBSI CCGCTC 1 cut(s) 606
AccII CGCG 1 cut(s) 10
AccIII TCCGGA 1 cut(s) 827
AclWI GGATC 8 cut(s) 286, 299, 299, 312, 377, 445, 454, 980
AcsI RAATTY 1 cut(s) 839
AcuI CTGAAG 1 cut(s) 204
AfaI GTAC 2 cut(s) 730, 869
AfiI CCNNNNNNNGG 4 cut(s) 73, 301, 776, 872
AflIII ACRYGT 1 cut(s) 811
AgsI TTSAA 4 cut(s) 349, 388, 541, 934
AhdI GACNNNNNGTC 1 cut(s) 90
AjiI CACGTC 1 cut(s) 35
AjnI CCWGG 1 cut(s) 246
AjuI GAANNNNNNNTTGG 2 cut(s) 460, 492
AluBI AGCT 3 cut(s) 338, 694, 897
AluI AGCT 3 cut(s) 338, 694, 897
Alw21I GWGCWC 2 cut(s) 429, 696
AlwI GGATC 8 cut(s) 286, 299, 299, 312, 377, 445, 454, 980
Ama87I CYCGRG 1 cut(s) 632
Aor13HI TCCGGA 1 cut(s) 827
AoxI GGCC 2 cut(s) 280, 778
ApeKI GCWGC 1 cut(s) 894
ApoI RAATTY 1 cut(s) 839
ArsI GACNNNNNNTTYG 2 cut(s) 831, 863
AspLEI GCGC 1 cut(s) 657
AspS9I GGNCC 1 cut(s) 280
AsuC2I CCSGG 1 cut(s) 379
AsuHPI GGTGA 3 cut(s) 16, 322, 889
AvaI CYCGRG 1 cut(s) 632
BaeGI GKGCMC 2 cut(s) 198, 214
BamHI GGATCC 2 cut(s) 291, 304
BanI GGYRCC 2 cut(s) 193, 209
BanII GRGCYC 2 cut(s) 51, 696
BauI CACGAG 1 cut(s) 339
Bbv12I GWGCWC 2 cut(s) 429, 696
BbvI GCAGC 1 cut(s) 906
BccI CCATC 6 cut(s) 168, 502, 578, 880, 962, 997
BceAI ACGGC 2 cut(s) 605, 886
BcgI CGANNNNNNTGC 2 cut(s) 151, 185
BciT130I CCWGG 1 cut(s) 248
BclI TGATCA 1 cut(s) 802
BcnI CCSGG 1 cut(s) 379
BfaI CTAG 1 cut(s) 689
BfoI RGCGCY 1 cut(s) 658
BglII AGATCT 2 cut(s) 155, 636
BisI GCNGC 5 cut(s) 319, 604, 758, 761, 895
BlsI GCNGC 5 cut(s) 320, 605, 759, 762, 896
Bme1390I CCNGG 2 cut(s) 248, 379
BmeRI GACNNNNNGTC 1 cut(s) 90
BmeT110I CYCGRG 1 cut(s) 632
BmgBI CACGTC 1 cut(s) 35
BmgT120I GGNCC 1 cut(s) 280
BmiI GGNNCC 8 cut(s) 50, 133, 195, 211, 293, 306, 362, 864
BmrFI CCNGG 2 cut(s) 248, 379
BmsI GCATC 3 cut(s) 493, 636, 1034
BpuMI CCSGG 1 cut(s) 379
Bsa29I ATCGAT 1 cut(s) 449
BsaBI GATNNNNATC 1 cut(s) 290
BsaJI CCNNGG 6 cut(s) 52, 67, 189, 364, 378, 467
BsaWI WCCGGW 5 cut(s) 307, 504, 714, 722, 827
BsaXI ACNNNNNCTCC 2 cut(s) 963, 993
Bsc4I CCNNNNNNNGG 4 cut(s) 73, 301, 776, 872
Bse118I RCCGGY 2 cut(s) 651, 737
Bse1I ACTGG 1 cut(s) 91
Bse8I GATNNNNATC 1 cut(s) 290
BseAI TCCGGA 1 cut(s) 827
BseBI CCWGG 1 cut(s) 248
BseCI ATCGAT 1 cut(s) 449
BseDI CCNNGG 6 cut(s) 52, 67, 189, 364, 378, 467
BseGI GGATG 5 cut(s) 179, 513, 570, 627, 919
BseJI GATNNNNATC 1 cut(s) 290
BseLI CCNNNNNNNGG 4 cut(s) 73, 301, 776, 872
BseMII CTCAG 1 cut(s) 36
BseNI ACTGG 1 cut(s) 91
BseRI GAGGAG 2 cut(s) 566, 701
BseSI GKGCMC 2 cut(s) 198, 214
BseXI GCAGC 1 cut(s) 906
BsgI GTGCAG 1 cut(s) 57
Bsh1236I CGCG 1 cut(s) 10
Bsh1285I CGRYCG 1 cut(s) 647
BshFI GGCC 2 cut(s) 282, 780
BshNI GGYRCC 2 cut(s) 193, 209
BshVI ATCGAT 1 cut(s) 449
BsiEI CGRYCG 1 cut(s) 647
BsiHKAI GWGCWC 2 cut(s) 429, 696
BsiHKCI CYCGRG 1 cut(s) 632
BsiWI CGTACG 1 cut(s) 867
BslFI GGGAC 2 cut(s) 45, 751
BslI CCNNNNNNNGG 4 cut(s) 73, 301, 776, 872
BsmFI GGGAC 2 cut(s) 45, 751
BsnI GGCC 2 cut(s) 282, 780
BsoBI CYCGRG 1 cut(s) 632
Bsp1286I GDGCHC 5 cut(s) 51, 198, 214, 429, 696
Bsp13I TCCGGA 1 cut(s) 827
BspANI GGCC 2 cut(s) 282, 780
BspCNI CTCAG 1 cut(s) 35
BspDI ATCGAT 1 cut(s) 449
BspEI TCCGGA 1 cut(s) 827
BspFNI CGCG 1 cut(s) 10
BspLI GGNNCC 8 cut(s) 50, 133, 195, 211, 293, 306, 362, 864
BspPI GGATC 8 cut(s) 286, 299, 299, 312, 377, 445, 454, 980
BspQI GCTCTTC 1 cut(s) 701
BspT107I GGYRCC 2 cut(s) 193, 209
BsrBI CCGCTC 1 cut(s) 606
BsrFI RCCGGY 2 cut(s) 651, 737
BsrI ACTGG 1 cut(s) 91
BssAI RCCGGY 2 cut(s) 651, 737
BssECI CCNNGG 6 cut(s) 52, 67, 189, 364, 378, 467
BssSI CACGAG 1 cut(s) 339
BssT1I CCWWGG 3 cut(s) 52, 189, 467
Bst2BI CACGAG 1 cut(s) 339
Bst2UI CCWGG 1 cut(s) 248
Bst4CI ACNGT 2 cut(s) 413, 867
Bst6I CTCTTC 2 cut(s) 701, 970
BstC8I GCNNGC 4 cut(s) 40, 653, 659, 739
BstDEI CTNAG 1 cut(s) 22
BstF5I GGATG 5 cut(s) 179, 513, 570, 627, 919
BstFNI CGCG 1 cut(s) 10
BstH2I RGCGCY 1 cut(s) 658
BstHHI GCGC 1 cut(s) 657
BstMCI CGRYCG 1 cut(s) 647
BstMWI GCNNNNNNNGC 1 cut(s) 159
BstNI CCWGG 1 cut(s) 248
BstNSI RCATGY 1 cut(s) 815
BstSCI CCNGG 2 cut(s) 246, 377
BstSLI GKGCMC 2 cut(s) 198, 214
BstUI CGCG 1 cut(s) 10
BstV1I GCAGC 1 cut(s) 906
BstX2I RGATCY 5 cut(s) 155, 291, 304, 369, 636
BstXI CCANNNNNNTGG 1 cut(s) 205
BstYI RGATCY 5 cut(s) 155, 291, 304, 369, 636
Bsu15I ATCGAT 1 cut(s) 449
BsuRI GGCC 2 cut(s) 282, 780
BsuTUI ATCGAT 1 cut(s) 449
BtrI CACGTC 1 cut(s) 35
BtsCI GGATG 5 cut(s) 179, 513, 570, 627, 919
Cac8I GCNNGC 4 cut(s) 40, 653, 659, 739
CfoI GCGC 1 cut(s) 657
Cfr10I RCCGGY 2 cut(s) 651, 737
Cfr13I GGNCC 1 cut(s) 280
ClaI ATCGAT 1 cut(s) 449
CseI GACGC 1 cut(s) 16
Csp6I GTAC 2 cut(s) 729, 868
CviAII CATG 4 cut(s) 116, 517, 678, 812
CviQI GTAC 2 cut(s) 729, 868
DdeI CTNAG 1 cut(s) 22
DriI GACNNNNNGTC 1 cut(s) 90
Eam1104I CTCTTC 2 cut(s) 701, 970
Eam1105I GACNNNNNGTC 1 cut(s) 90
EarI CTCTTC 2 cut(s) 701, 970
Ecl136II GAGCTC 1 cut(s) 694
Eco130I CCWWGG 3 cut(s) 52, 189, 467
Eco24I GRGCYC 2 cut(s) 51, 696
Eco53kI GAGCTC 1 cut(s) 694
Eco57I CTGAAG 1 cut(s) 204
Eco88I CYCGRG 1 cut(s) 632
EcoICRI GAGCTC 1 cut(s) 694
EcoRI GAATTC 1 cut(s) 839
EcoRII CCWGG 1 cut(s) 246
EcoT14I CCWWGG 3 cut(s) 52, 189, 467
EcoT22I ATGCAT 1 cut(s) 1027
EcoT38I GRGCYC 2 cut(s) 51, 696
ErhI CCWWGG 3 cut(s) 52, 189, 467
FaeI CATG 4 cut(s) 119, 520, 681, 815
FaiI YATR 8 cut(s) 81, 117, 498, 518, 679, 813, 900, 1062
FaqI GGGAC 2 cut(s) 45, 751
FatI CATG 4 cut(s) 115, 516, 677, 811
FauI CCCGC 3 cut(s) 462, 574, 866
FbaI TGATCA 1 cut(s) 802
Fnu4HI GCNGC 5 cut(s) 319, 604, 758, 761, 895
FokI GGATG 5 cut(s) 186, 520, 557, 614, 926
FriOI GRGCYC 2 cut(s) 51, 696
Fsp4HI GCNGC 5 cut(s) 319, 604, 758, 761, 895
FspBI CTAG 1 cut(s) 689
GlaI GCGC 1 cut(s) 656
GluI GCNGC 5 cut(s) 319, 604, 758, 761, 895
HaeII RGCGCY 1 cut(s) 658
HaeIII GGCC 2 cut(s) 282, 780
HgaI GACGC 1 cut(s) 16
HhaI GCGC 1 cut(s) 657
Hin1II CATG 4 cut(s) 119, 520, 681, 815
Hin6I GCGC 1 cut(s) 655
HinP1I GCGC 1 cut(s) 655
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HinfI GANTC 5 cut(s) 112, 275, 476, 533, 750
HphI GGTGA 3 cut(s) 16, 322, 889
Hpy166II GTNNAC 2 cut(s) 121, 253
Hpy188I TCNGA 5 cut(s) 25, 173, 221, 365, 644
Hpy188III TCNNGA 4 cut(s) 341, 373, 689, 828
Hpy8I GTNNAC 2 cut(s) 121, 253
Hpy99I CGWCG 4 cut(s) 15, 413, 795, 798
HpyAV CCTTC 2 cut(s) 541, 874
HpyCH4III ACNGT 2 cut(s) 413, 867
HpyCH4IV ACGT 3 cut(s) 13, 34, 796
HpyCH4V TGCA 4 cut(s) 38, 153, 484, 1025
HpyF10VI GCNNNNNNNGC 1 cut(s) 159
HpyF3I CTNAG 1 cut(s) 22
HpySE526I ACGT 3 cut(s) 13, 34, 796
Hsp92II CATG 4 cut(s) 119, 520, 681, 815
HspAI GCGC 1 cut(s) 655
Kpn2I TCCGGA 1 cut(s) 827
KroI GCCGGC 2 cut(s) 651, 737
KroNI GCCGGC 2 cut(s) 653, 739
Ksp22I TGATCA 1 cut(s) 802
LguI GCTCTTC 1 cut(s) 701
LmnI GCTCC 2 cut(s) 54, 366
Lsp1109I GCAGC 1 cut(s) 906
LweI GCATC 3 cut(s) 493, 636, 1034
MaeI CTAG 1 cut(s) 689
MaeII ACGT 3 cut(s) 13, 34, 796
MaeIII GTNAC 1 cut(s) 4
MbiI CCGCTC 1 cut(s) 606
MboII GAAGA 4 cut(s) 631, 688, 863, 987
MfeI CAATTG 1 cut(s) 388
MflI RGATCY 5 cut(s) 155, 291, 304, 369, 636
MhlI GDGCHC 5 cut(s) 51, 198, 214, 429, 696
MluCI AATT 4 cut(s) 388, 669, 839, 919
MlyI GAGTC 2 cut(s) 121, 542
MmeI TCCRAC 4 cut(s) 151, 580, 667, 984
Mph1103I ATGCAT 1 cut(s) 1027
MreI CGCCGGCG 1 cut(s) 737
MroI TCCGGA 1 cut(s) 827
MroNI GCCGGC 2 cut(s) 651, 737
MseI TTAA 1 cut(s) 1075
MslI CAYNNNNRTG 1 cut(s) 816
MspA1I CMGCKG 1 cut(s) 763
MspR9I CCNGG 2 cut(s) 248, 379
MunI CAATTG 1 cut(s) 388
MvaI CCWGG 1 cut(s) 248
MvnI CGCG 1 cut(s) 10
MwoI GCNNNNNNNGC 1 cut(s) 159
NaeI GCCGGC 2 cut(s) 653, 739
NciI CCSGG 1 cut(s) 379
NgoMIV GCCGGC 2 cut(s) 651, 737
NlaIII CATG 4 cut(s) 119, 520, 681, 815
NlaIV GGNNCC 8 cut(s) 50, 133, 195, 211, 293, 306, 362, 864
NmuCI GTSAC 1 cut(s) 4
NsiI ATGCAT 1 cut(s) 1027
NspI RCATGY 1 cut(s) 815
PciI ACATGT 1 cut(s) 811
PciSI GCTCTTC 1 cut(s) 701
PdiI GCCGGC 2 cut(s) 653, 739
PfeI GAWTC 3 cut(s) 275, 476, 750
Pfl23II CGTACG 1 cut(s) 867
PkrI GCNGC 5 cut(s) 320, 605, 759, 762, 896
PleI GAGTC 2 cut(s) 120, 541
PpsI GAGTC 2 cut(s) 120, 541
PscI ACATGT 1 cut(s) 811
Psp124BI GAGCTC 1 cut(s) 696
Psp6I CCWGG 1 cut(s) 246
PspGI CCWGG 1 cut(s) 246
PspLI CGTACG 1 cut(s) 867
PspN4I GGNNCC 8 cut(s) 50, 133, 195, 211, 293, 306, 362, 864
PspPI GGNCC 1 cut(s) 280
PsuI RGATCY 5 cut(s) 155, 291, 304, 369, 636
RsaI GTAC 2 cut(s) 730, 869
RsaNI GTAC 2 cut(s) 729, 868
RseI CAYNNNNRTG 1 cut(s) 816
SacI GAGCTC 1 cut(s) 696
SapI GCTCTTC 1 cut(s) 701
SaqAI TTAA 1 cut(s) 1075
SatI GCNGC 5 cut(s) 319, 604, 758, 761, 895
Sau96I GGNCC 1 cut(s) 280
SchI GAGTC 2 cut(s) 121, 542
ScrFI CCNGG 2 cut(s) 248, 379
SduI GDGCHC 5 cut(s) 51, 198, 214, 429, 696
SfaNI GCATC 3 cut(s) 493, 636, 1034
SgrAI CRCCGGYG 1 cut(s) 737
SmiMI CAYNNNNRTG 1 cut(s) 816
Sse9I AATT 4 cut(s) 388, 669, 839, 919
SspMI CTAG 1 cut(s) 689
SstI GAGCTC 1 cut(s) 696
StyD4I CCNGG 2 cut(s) 246, 377
StyI CCWWGG 3 cut(s) 52, 189, 467
TaaI ACNGT 2 cut(s) 413, 867
TaiI ACGT 3 cut(s) 16, 37, 799
TaqI TCGA 5 cut(s) 408, 449, 474, 837, 849
TaqII GACCGA 1 cut(s) 661
TasI AATT 4 cut(s) 388, 669, 839, 919
TauI GCSGC 4 cut(s) 321, 606, 760, 763
TfiI GAWTC 3 cut(s) 275, 476, 750
Tru1I TTAA 1 cut(s) 1075
Tru9I TTAA 1 cut(s) 1075
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 1 cut(s) 894
Tsp45I GTSAC 1 cut(s) 4
TspGWI ACGGA 1 cut(s) 681
XapI RAATTY 1 cut(s) 839
XbaI TCTAGA 1 cut(s) 688
XceI RCATGY 1 cut(s) 815
XspI CTAG 1 cut(s) 689
Zsp2I ATGCAT 1 cut(s) 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.