Rmu_sc0000977.1_g000020

Pentatricopeptide repeat-containing protein At2g13420, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000977.1
OG0006370
Physical Location & Seq
Forward (+)
94145 .. 95350
1206 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1206 bp
atggcacaatgttgcttctcctcctctcttcttcgttttctccgttcagctcgccggttctcctcactccccaacccaacaacaacgacccaaaacgacgccgaactggtatcaaacatcctcctccaccaccacaacccctttcacgccatggagtcctctctccagctccacggcatcactctctcccccgacctcctccaccacactctcctccgcctcaagcacaattccaaaatcgccctcgccctcttccactacgccaagtccctcccctcccccccgcccctcacctcccacgccttccacctcctcatcgacatcgtcgccaaggtccgccagtacgacgtcgcttggcagctcatcctcgaaatggaccgccacaacctcactcccaccgccgccaccttcctcatcctcatccgccgcctcatcgcctccggcctcacccgccaggccgtccgggccttcgacgacatcgagaccttcgtcctgacccgccccaccgcccaagacttctgcttcttactcgacaccctctgtaaatacggctacgttaagctcgcggctgaagttttcaacaaaaggaagcatgactatgtggccgatgtgaaaatgtacactgttctggtttacgggtggtgtaagatcggccgtttcgatatggcggagaggttcttgagggatatgatcgagcgaggtgttgagcccaatgtggttacttacaatgtgtttctggatgggatttgtaggaaggcgagtttgcaccctcaggacaggtttgagagaaccataagaaatgcactgaaggtgttcgacgaaatgtccagcagaggcattgagccggacgtgacgagtttttcgatccttcttcatgtttatagccgggcgcataagcccgagttgtcgcttgataagctcaaggaaatgagggagagggggatttgccccaccgtggcgacttacacttcagttgttaagtgtctttgctcttgcgggaggcttgaggaagcggaggagttgcttggtgagatggtgaggaatggggttagtccctctgcggctacgtataattgtttcttcaaagagtacagggggagaaaggactcggaaagtgctttgaagttgtataggaagatgaaggaggatagattgtttgcgccgagtgtgcacacttataatatattggtggggatacacttataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

401

Amino Acids

45.94

Weight (kDa)

9.02

Isoelectric Point (pI)

46.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 101 - 144 5.9e-06 PPR repeat family
PPR_2 PF13041 203 - 251 6.5e-14 PPR repeat family
PPR_long PF17177 229 - 358 8.5e-12 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 265 - 294 4.1e-07 PPR repeat family
PPR_3 PF13812 270 - 327 8e-11 Pentatricopeptide repeat domain
PPR_2 PF13041 288 - 330 1.3e-07 PPR repeat family
TPR_24 PF23276 291 - 383 2.5e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 313 - 346 2.5e-11 PPR repeat
PPR_2 PF13041 317 - 363 1.4e-12 PPR repeat family
PPR PF01535 321 - 350 1.4e-08 PPR repeat
PPR_3 PF13812 341 - 399 1.7e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 356 - 397 2.2e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1179, 1204
AatII GACGTC 1 cut(s) 351
AccII CGCG 1 cut(s) 566
AclWI GGATC 1 cut(s) 859
AcoI YGGCCR 2 cut(s) 603, 652
AcuI CTGAAG 3 cut(s) 591, 827, 954
AcyI GRCGYC 2 cut(s) 99, 348
AfaI GTAC 3 cut(s) 344, 620, 1091
AfiI CCNNNNNNNGG 2 cut(s) 373, 955
AgsI TTSAA 3 cut(s) 580, 1084, 1123
AhdI GACNNNNNGTC 1 cut(s) 823
AjiI CACGTC 1 cut(s) 850
AjnI CCWGG 1 cut(s) 453
AluBI AGCT 5 cut(s) 50, 169, 361, 562, 919
AluI AGCT 5 cut(s) 50, 169, 361, 562, 919
Alw21I GWGCWC 1 cut(s) 1173
Alw26I GTCTC 1 cut(s) 476
Alw44I GTGCAC 1 cut(s) 1169
AlwI GGATC 1 cut(s) 859
Ama87I CYCGRG 1 cut(s) 899
AoxI GGCC 5 cut(s) 442, 456, 465, 603, 652
ApaLI GTGCAC 1 cut(s) 1169
ApeKI GCWGC 1 cut(s) 358
ArsI GACNNNNNNTTYG 2 cut(s) 844, 876
Asp700I GAANNNNTTC 1 cut(s) 812
AspLEI GCGC 2 cut(s) 892, 1162
AspS9I GGNCC 3 cut(s) 334, 376, 465
AsuC2I CCSGG 2 cut(s) 464, 887
AsuHPI GGTGA 4 cut(s) 283, 439, 1040, 1048
AvaI CYCGRG 1 cut(s) 899
AvaII GGWCC 2 cut(s) 334, 376
AxyI CCTNAGG 1 cut(s) 771
BaeGI GKGCMC 1 cut(s) 1173
BanII GRGCYC 1 cut(s) 711
Bbv12I GWGCWC 1 cut(s) 1173
BbvI GCAGC 1 cut(s) 370
BccI CCATC 2 cut(s) 734, 1027
BceAI ACGGC 4 cut(s) 190, 443, 565, 639
BciT130I CCWGG 1 cut(s) 455
BciVI GTATCC 1 cut(s) 1188
BcnI CCSGG 2 cut(s) 464, 887
BcoDI GTCTC 1 cut(s) 476
BfuI GTATCC 1 cut(s) 1188
BglI GCCNNNNNGGC 1 cut(s) 464
BisI GCNGC 5 cut(s) 359, 402, 427, 567, 1062
BlsI GCNGC 5 cut(s) 360, 403, 428, 568, 1063
Bme1390I CCNGG 3 cut(s) 455, 464, 887
Bme18I GGWCC 2 cut(s) 334, 376
BmeRI GACNNNNNGTC 1 cut(s) 823
BmeT110I CYCGRG 1 cut(s) 899
BmgBI CACGTC 1 cut(s) 850
BmgT120I GGNCC 3 cut(s) 334, 376, 465
BmrFI CCNGG 3 cut(s) 455, 464, 887
BmsI GCATC 1 cut(s) 186
BoxI GACNNNNGTC 1 cut(s) 488
BpmI CTGGAG 1 cut(s) 149
BpuEI CTTGAG 4 cut(s) 206, 700, 905, 1025
BpuMI CCSGG 2 cut(s) 464, 887
BsaAI YACGTR 1 cut(s) 1068
BsaHI GRCGYC 2 cut(s) 99, 348
BsaI GGTCTC 1 cut(s) 476
BsaJI CCNNGG 4 cut(s) 150, 172, 330, 954
Bsc4I CCNNNNNNNGG 2 cut(s) 373, 955
Bse118I RCCGGY 1 cut(s) 54
Bse1I ACTGG 2 cut(s) 111, 340
Bse21I CCTNAGG 1 cut(s) 771
BseBI CCWGG 1 cut(s) 455
BseDI CCNNGG 4 cut(s) 150, 172, 330, 954
BseGI GGATG 5 cut(s) 117, 363, 414, 420, 745
BseLI CCNNNNNNNGG 2 cut(s) 373, 955
BseMII CTCAG 1 cut(s) 785
BseNI ACTGG 2 cut(s) 111, 340
BseRI GAGGAG 8 cut(s) 10, 13, 52, 113, 188, 203, 302, 1031
BseSI GKGCMC 1 cut(s) 1173
BseX3I CGGCCG 1 cut(s) 652
BseXI GCAGC 1 cut(s) 370
Bsh1236I CGCG 1 cut(s) 566
Bsh1285I CGRYCG 1 cut(s) 655
BshFI GGCC 5 cut(s) 444, 458, 467, 605, 654
BsiEI CGRYCG 1 cut(s) 655
BsiHKAI GWGCWC 1 cut(s) 1173
BsiHKCI CYCGRG 1 cut(s) 899
BsiSI CCGG 5 cut(s) 55, 441, 463, 845, 886
BslFI GGGAC 2 cut(s) 253, 1038
BslI CCNNNNNNNGG 2 cut(s) 373, 955
BsmAI GTCTC 1 cut(s) 476
BsmFI GGGAC 2 cut(s) 253, 1038
BsnI GGCC 5 cut(s) 444, 458, 467, 605, 654
Bso31I GGTCTC 1 cut(s) 476
BsoBI CYCGRG 1 cut(s) 899
Bsp1286I GDGCHC 2 cut(s) 711, 1173
Bsp1407I TGTACA 1 cut(s) 618
Bsp143I GATC 3 cut(s) 648, 690, 864
Bsp19I CCATGG 1 cut(s) 150
BspANI GGCC 5 cut(s) 444, 458, 467, 605, 654
BspCNI CTCAG 1 cut(s) 784
BspFNI CGCG 1 cut(s) 566
BspPI GGATC 1 cut(s) 859
BspTNI GGTCTC 1 cut(s) 476
BsrFI RCCGGY 1 cut(s) 54
BsrGI TGTACA 1 cut(s) 618
BsrI ACTGG 2 cut(s) 111, 340
BssAI RCCGGY 1 cut(s) 54
BssECI CCNNGG 4 cut(s) 150, 172, 330, 954
BssMI GATC 3 cut(s) 648, 690, 864
BssNI GRCGYC 2 cut(s) 99, 348
BssT1I CCWWGG 2 cut(s) 150, 330
Bst2UI CCWGG 1 cut(s) 455
Bst4CI ACNGT 2 cut(s) 625, 955
Bst6I CTCTTC 2 cut(s) 33, 257
BstACI GRCGYC 2 cut(s) 99, 348
BstAUI TGTACA 1 cut(s) 618
BstBAI YACGTR 1 cut(s) 1068
BstC8I GCNNGC 2 cut(s) 52, 564
BstDEI CTNAG 1 cut(s) 771
BstDSI CCRYGG 3 cut(s) 150, 172, 954
BstF5I GGATG 5 cut(s) 117, 363, 414, 420, 745
BstFNI CGCG 1 cut(s) 566
BstHHI GCGC 2 cut(s) 892, 1162
BstKTI GATC 3 cut(s) 651, 693, 867
BstMAI GTCTC 1 cut(s) 476
BstMBI GATC 3 cut(s) 648, 690, 864
BstMCI CGRYCG 1 cut(s) 655
BstMWI GCNNNNNNNGC 4 cut(s) 450, 464, 916, 1168
BstNI CCWGG 1 cut(s) 455
BstPAI GACNNNNGTC 1 cut(s) 488
BstSCI CCNGG 3 cut(s) 453, 462, 885
BstSLI GKGCMC 1 cut(s) 1173
BstSNI TACGTA 1 cut(s) 1068
BstUI CGCG 1 cut(s) 566
BstV1I GCAGC 1 cut(s) 370
BstZI CGGCCG 1 cut(s) 652
Bsu36I CCTNAGG 1 cut(s) 771
BsuI GTATCC 1 cut(s) 1188
BsuRI GGCC 5 cut(s) 444, 458, 467, 605, 654
BtgI CCRYGG 3 cut(s) 150, 172, 954
BtgZI GCGATG 1 cut(s) 418
BtrI CACGTC 1 cut(s) 850
BtsCI GGATG 5 cut(s) 117, 363, 414, 420, 745
BtsIMutI CAGTG 2 cut(s) 621, 803
Cac8I GCNNGC 2 cut(s) 52, 564
CfoI GCGC 2 cut(s) 892, 1162
Cfr10I RCCGGY 1 cut(s) 54
Cfr13I GGNCC 3 cut(s) 334, 376, 465
CseI GACGC 1 cut(s) 107
Csp6I GTAC 3 cut(s) 343, 619, 1090
CviAII CATG 3 cut(s) 151, 593, 875
CviQI GTAC 3 cut(s) 343, 619, 1090
DdeI CTNAG 1 cut(s) 771
DpnI GATC 3 cut(s) 650, 692, 866
DpnII GATC 3 cut(s) 648, 690, 864
DriI GACNNNNNGTC 1 cut(s) 823
EaeI YGGCCR 2 cut(s) 603, 652
EagI CGGCCG 1 cut(s) 652
Eam1104I CTCTTC 2 cut(s) 33, 257
Eam1105I GACNNNNNGTC 1 cut(s) 823
EarI CTCTTC 2 cut(s) 33, 257
EciI GGCGGA 4 cut(s) 206, 326, 413, 683
EclXI CGGCCG 1 cut(s) 652
Eco105I TACGTA 1 cut(s) 1068
Eco130I CCWWGG 2 cut(s) 150, 330
Eco24I GRGCYC 1 cut(s) 711
Eco31I GGTCTC 1 cut(s) 476
Eco47I GGWCC 2 cut(s) 334, 376
Eco52I CGGCCG 1 cut(s) 652
Eco57I CTGAAG 3 cut(s) 591, 827, 954
Eco81I CCTNAGG 1 cut(s) 771
Eco88I CYCGRG 1 cut(s) 899
EcoRII CCWGG 1 cut(s) 453
EcoT14I CCWWGG 2 cut(s) 150, 330
EcoT38I GRGCYC 1 cut(s) 711
ErhI CCWWGG 2 cut(s) 150, 330
FaeI CATG 3 cut(s) 154, 596, 878
FaqI GGGAC 2 cut(s) 253, 1038
FatI CATG 3 cut(s) 150, 592, 874
FauI CCCGC 4 cut(s) 291, 458, 506, 989
Fnu4HI GCNGC 5 cut(s) 359, 402, 427, 567, 1062
FokI GGATG 5 cut(s) 104, 350, 401, 407, 752
FriOI GRGCYC 1 cut(s) 711
Fsp4HI GCNGC 5 cut(s) 359, 402, 427, 567, 1062
GlaI GCGC 2 cut(s) 891, 1161
GluI GCNGC 5 cut(s) 359, 402, 427, 567, 1062
GsuI CTGGAG 1 cut(s) 149
HaeIII GGCC 5 cut(s) 444, 458, 467, 605, 654
HapII CCGG 5 cut(s) 55, 441, 463, 845, 886
HgaI GACGC 1 cut(s) 107
HhaI GCGC 2 cut(s) 892, 1162
Hin1I GRCGYC 2 cut(s) 99, 348
Hin1II CATG 3 cut(s) 154, 596, 878
Hin6I GCGC 2 cut(s) 890, 1160
HinP1I GCGC 2 cut(s) 890, 1160
HinfI GANTC 2 cut(s) 155, 1106
HpaII CCGG 5 cut(s) 55, 441, 463, 845, 886
HphI GGTGA 4 cut(s) 283, 439, 1040, 1048
Hpy166II GTNNAC 3 cut(s) 621, 634, 1171
Hpy188I TCNGA 1 cut(s) 1111
Hpy188III TCNNGA 5 cut(s) 481, 493, 679, 737, 773
Hpy8I GTNNAC 3 cut(s) 621, 634, 1171
Hpy99I CGWCG 6 cut(s) 101, 329, 350, 353, 476, 821
HpyAV CCTTC 8 cut(s) 313, 418, 478, 496, 748, 802, 878, 1135
HpyCH4III ACNGT 2 cut(s) 625, 955
HpyCH4IV ACGT 4 cut(s) 348, 555, 849, 1067
HpyCH4V TGCA 3 cut(s) 766, 803, 1171
HpyF10VI GCNNNNNNNGC 4 cut(s) 450, 464, 916, 1168
HpyF3I CTNAG 1 cut(s) 771
HpySE526I ACGT 4 cut(s) 348, 555, 849, 1067
Hsp92I GRCGYC 2 cut(s) 99, 348
Hsp92II CATG 3 cut(s) 154, 596, 878
HspAI GCGC 2 cut(s) 890, 1160
Kzo9I GATC 3 cut(s) 648, 690, 864
LmnI GCTCC 1 cut(s) 174
Lsp1109I GCAGC 1 cut(s) 370
LweI GCATC 1 cut(s) 186
MaeII ACGT 4 cut(s) 348, 555, 849, 1067
MaeIII GTNAC 2 cut(s) 718, 850
MalI GATC 3 cut(s) 650, 692, 866
MboI GATC 3 cut(s) 648, 690, 864
MboII GAAGA 6 cut(s) 20, 23, 244, 863, 1072, 1147
MhlI GDGCHC 2 cut(s) 711, 1173
MluCI AATT 2 cut(s) 229, 1072
MlyI GAGTC 2 cut(s) 164, 1100
MroXI GAANNNNTTC 1 cut(s) 812
MseI TTAA 2 cut(s) 558, 978
MslI CAYNNNNRTG 1 cut(s) 597
MspI CCGG 5 cut(s) 55, 441, 463, 845, 886
MspR9I CCNGG 3 cut(s) 455, 464, 887
MvaI CCWGG 1 cut(s) 455
MvnI CGCG 1 cut(s) 566
MwoI GCNNNNNNNGC 4 cut(s) 450, 464, 916, 1168
NciI CCSGG 2 cut(s) 464, 887
NcoI CCATGG 1 cut(s) 150
NdeII GATC 3 cut(s) 648, 690, 864
NlaIII CATG 3 cut(s) 154, 596, 878
NmeAIII GCCGAG 1 cut(s) 1188
NmuCI GTSAC 1 cut(s) 850
PcsI WCGNNNNNNNCGW 5 cut(s) 40, 477, 486, 657, 860
PdmI GAANNNNTTC 1 cut(s) 812
PflFI GACNNNGTC 1 cut(s) 323
PkrI GCNGC 5 cut(s) 360, 403, 428, 568, 1063
PleI GAGTC 2 cut(s) 163, 1100
PpsI GAGTC 2 cut(s) 163, 1100
Ppu21I YACGTR 1 cut(s) 1068
PshAI GACNNNNGTC 1 cut(s) 488
PsiI TTATAA 2 cut(s) 1179, 1204
Psp6I CCWGG 1 cut(s) 453
PspGI CCWGG 1 cut(s) 453
PspPI GGNCC 3 cut(s) 334, 376, 465
PsyI GACNNNGTC 1 cut(s) 323
RsaI GTAC 3 cut(s) 344, 620, 1091
RsaNI GTAC 3 cut(s) 343, 619, 1090
RseI CAYNNNNRTG 1 cut(s) 597
SaqAI TTAA 2 cut(s) 558, 978
SatI GCNGC 5 cut(s) 359, 402, 427, 567, 1062
Sau3AI GATC 3 cut(s) 648, 690, 864
Sau96I GGNCC 3 cut(s) 334, 376, 465
SchI GAGTC 2 cut(s) 164, 1100
ScrFI CCNGG 3 cut(s) 455, 464, 887
SduI GDGCHC 2 cut(s) 711, 1173
SfaNI GCATC 1 cut(s) 186
SfiI GGCCNNNNNGGCC 1 cut(s) 464
SinI GGWCC 2 cut(s) 334, 376
SmiMI CAYNNNNRTG 1 cut(s) 597
SmlI CTYRAG 4 cut(s) 221, 679, 920, 1004
SmoI CTYRAG 4 cut(s) 221, 679, 920, 1004
SnaBI TACGTA 1 cut(s) 1068
Sse9I AATT 2 cut(s) 229, 1072
StyD4I CCNGG 3 cut(s) 453, 462, 885
StyI CCWWGG 2 cut(s) 150, 330
TaaI ACNGT 2 cut(s) 625, 955
TaiI ACGT 4 cut(s) 351, 558, 852, 1070
TaqI TCGA 9 cut(s) 318, 369, 471, 480, 531, 660, 693, 816, 863
TasI AATT 2 cut(s) 229, 1072
TatI WGTACW 2 cut(s) 618, 1089
TauI GCSGC 4 cut(s) 404, 429, 569, 1064
Tru1I TTAA 2 cut(s) 558, 978
Tru9I TTAA 2 cut(s) 558, 978
TscAI CASTG 2 cut(s) 628, 810
TseFI GTSAC 1 cut(s) 850
TseI GCWGC 1 cut(s) 358
Tsp45I GTSAC 1 cut(s) 850
TspDTI ATGAA 2 cut(s) 863, 1154
TspGWI ACGGA 1 cut(s) 32
TspRI CASTG 2 cut(s) 628, 810
Tth111I GACNNNGTC 1 cut(s) 323
VneI GTGCAC 1 cut(s) 1169
VpaK11BI GGWCC 2 cut(s) 334, 376
XmnI GAANNNNTTC 1 cut(s) 812
ZraI GACGTC 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.